Brazilian Journal of Pharmaceutical Sciences
versão On-line ISSN 2175-9790
MANCINI, Dalva Assunção Portari; TORRES, Rosângela Pavan; PINTO, José Ricardo e MANCINI-FILHO, Jorge. Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice. Braz. J. Pharm. Sci. [online]. 2009, vol.45, n.1, pp.127-133. ISSN 2175-9790. http://dx.doi.org/10.1590/S1984-82502009000100016.
This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID50/mL of the HSV-1 was kept for 3 h at 4º C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37º C in CO2-5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The HSV-1 replication inhibition percentage (IP) measured the antiviral action from antioxidants, showing viral reductions of the 82.0, 82.5%, in the presence of rosemary and rosemary + BHT, respectively. As an extension, cell test corresponded to the similar viral decrease (IP = 85.0 and 86.3%) in both aforementioned situations. Results lead to conclude that phenolic compounds from rosemary revealed an antiviral action on herpesvirus-1.
Palavras-chave : Natural antioxidants; Virus replication; Phenolic compounds; Herpesvirus; Rosemary.