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Brazilian Journal of Pharmaceutical Sciences
On-line version ISSN 2175-9790
COLLI, Cristiane Maria; ELEFANT, Guita Rubinsky; PALUDO, Marcia Liz and GUILHERME, Ana Lúcia Falavigna. An alternate technique for isolation of Toxocara canis excretory-secretory antigens. Braz. J. Pharm. Sci. [online]. 2011, vol.47, n.1, pp.119-123. ISSN 2175-9790. http://dx.doi.org/10.1590/S1984-82502011000100015.
The aim of the present study was to test the effectiveness of a sausage-casing membrane for dialysis of Toxocara excretory-secretory antigens (TES). The protein concentrated by the tested membrane was compared with that obtained using a Sigma commercial membrane, as were the protein fractions found by polyacrylamide gel electrophoresis. Standard positive and negative serum samples were evaluated in an ELISA immunoassay, and equivalent data were obtained in all steps, indicating that the sausage-casing membrane is efficient, besides being less expensive to process.
Keywords : Toxocara canis [excretory-secretory antigens]; Antigens [isolation]; Dialysis.