Brazilian Journal of Pharmaceutical Sciences
Print version ISSN 1984-8250
SARAIVA, Antonio Marcos et al. Antimicrobial activity and bioautographic study of antistaphylococcal components from Caesalpinia pyramidalis Tull.. Braz. J. Pharm. Sci. [online]. 2012, vol.48, n.1, pp. 147-154. ISSN 1984-8250. http://dx.doi.org/10.1590/S1984-82502012000100016.
The antimicrobial activity of dry methanol and ethyl acetate extracts for the leaves, bark of the stem, peel of the root, flower, fruit and seed of Caesalpinia pyramidalis Tull. (catingueira) was performed against seventeen isolates of Staphylococcus aureus MRSA multiresistant strains, which included two isolates of S. aureus MSSA and two ATCC strains. The antimicrobial activity was tested by the agar diffusion method and the Minimum Inhibitory Concentration (MIC) was determined. The dry methanol extract of the root showed good antimicrobial activity with a MIC of less than 0.5 mg.mL-1. The dry ethyl acetate extracts exhibited lower antimicrobial activity, which might be explained by solubility problems and less diffusion in the agar medium. Results of the bioautographies also confirmed inhibition halos corresponding to the active substances present in the leaves, as well as in the flower of C. pyramidalis. The phytochemical study of the leaves, bark of the stem, peel of the root, flower and fruit of extracts from C. pyramidalis confirmed the presence of a number of known antimicrobial agents including ursolic acid, quercetin, catechin, ellagic acid, sitosterol, flavonoids, proanthocyanidins and gallic acid.
Keywords : Caesalpinia pyramidalis [antimicrobial activity]; Caesalpinia pyramidalis [bioautographic study]; Caesalpinia pyramidalis [antistaphylococcal components]; Staphylococcus aureus [multiresistant]; Brazilian Epidemic Clone.