Brazilian Journal of Pharmaceutical Sciences
On-line version ISSN 2175-9790
CHENG, Zhongzhe et al. HPLC method for the simultaneous determination of four compounds in rat plasma after intravenous administration of Portulaca oleracea L. extract. Braz. J. Pharm. Sci. [online]. 2012, vol.48, n.1, pp.163-170. ISSN 2175-9790. http://dx.doi.org/10.1590/S1984-82502012000100018.
The objective of the present study was to develop a simple and selective HPLC method for the simultaneous determination of hesperidin (HP), caffeic acid (CA), ferulic acid (FA) and p-coumaric acid (p-CA) in rat plasma after intravenous administration of Portulaca oleracea L. extract (POE). With the hyperoside as the internal standard, the sample pretreatment procedure involved simple single-step extraction with methanol of 0.2 mL plasma. The mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-0.5% glacial acetic acid (5:3:18:74, v/v/v/v). The calibration curves were linear over the range of 0.1-25 µg mL-1, 0.1-25 µg mL-1, 0.1-25 µg mL-1and 0.015-3 µg mL-1 for HP, CA, FA and p-CA, respectively. The method developed was suitable for the pharmacokinetic study of HP, CA, FA and p-CA in rats after intravenous administration of POE.
Keywords : Portulaca oleracea L. [pharmacognosy]; High Performance Liquid Chromatography [rat plasma]; Vegetal extract [pharmacokinetic study]; Medicinal plants.