SciELO - Scientific Electronic Library Online

vol.50 número3Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studiesByproducts of orange extraction: influence of different treatments in fiber composition and physical and chemical parameters índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Brazilian Journal of Pharmaceutical Sciences

versão impressa ISSN 1984-8250versão On-line ISSN 2175-9790


LEITE, Gisela Myrian de Lima et al. Lack of effect of cell-wall targeted antibacterials on biofilm formation and antifungal susceptibility of Candidaspecies. Braz. J. Pharm. Sci. [online]. 2014, vol.50, n.3, pp.467-472. ISSN 1984-8250.

The use of central venous catheters (CVC) and broad-spectrum antibacterials are among the main risk factors for the development of candidemia in patients admitted to intensive care units (ICU). It is known that some antibacterials increase the resistance of these yeasts to azole antifungals. Thus, the aim of this research was to determine whether yeast present in CVC colonizations previously exposed to cell-wall targeted antibacterials benefit from a reduction in susceptibility to fluconazole and voriconazole, facilitating their ability to form biofilms. Candida albicans, C. tropicalis, C. glabrata, C. parapsilosis and C. guilhermondii were seeded into antibacterial (cefepime, meropenem, vancomycin, and piperacillin-tazobactam) gradient plates produced in Mueller-Hinton Agar. The susceptibility to fluconazole and voriconazole and the biofilm formation of the yeasts were tested before and after exposure to the antibacterials. None of the antibacterials exerted a significant effect on the in vitro susceptibility of the yeasts to the antifungal agents or on their ability to form biofilms. These results suggest that increased candidemia in ICU patients is not attributable to possible alterations in the yeasts, but is more likely caused by a weakening of the patient's general condition after long exposure to infection.

Palavras-chave : Central venous cateter/use; Candidemia/control; Biofilm/formation; Antibacterials; Antifungals; Candida/species/control.

        · resumo em Português     · texto em Inglês     · Inglês ( pdf )