ABSTRACT
The aim of this study was to evaluate the effect of Ceratocystis fimbriata isolates, obtained from Actinidia deliciosa (kiwifruit), on black wattle (Acacia mearnsii De Wild.) seedlings, the potential biological control exerted in vitro by Bacillus subtilis on C. fimbriata, and the in vivo effect of B. subtilis against C. fimbriata on black wattle seedlings. Isolates of C. fimbriata obtained and identified from kiwi plantations were used in pathogenicity tests on A. mearnsii seedlings. In vitro, the antagonistic potential of B. subtilis on the pathogen was analyzed by direct confrontation tests. To evaluate the effect of B. subtilis in vivo, black wattle seedlings were inoculated with Rhizolyptus® seven days before and after pathogen inoculation. On black wattle seedlings, C. fimbriata isolates caused 44,15% to 100% severity. Direct confrontation tests revealed that the strains were effective in the biological control of C. fimbriata isolates in vitro. In vivo, Rizolyptus® was not efficient in controlling C. fimbriata.
Keywords:
fungus; wilt; bioprotector
