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Hoehnea

versão On-line ISSN 2236-8906

Resumo

AMARAL, Lourdes Isabel Velho do et al. A new rapid and sensitive enzymatic method for extraction and quantification of starch in plant material. Hoehnea [online]. 2007, vol.34, n.4, pp.425-431. ISSN 2236-8906.  http://dx.doi.org/10.1590/S2236-89062007000400001.

In this work we compare methods normally used for starch determination in plant materials. The comparison between chemical (McCready's method) and an enzymatic method proposed here showed that although McCready's method is appropriate for most plant materials, in certain cases where cell wall polysaccharides (pectins and hemicelluloses) are present, the results may be significantly altered. However, using the enzymatic method described here afforded accurate estimation of starch content in such tissues. The enzymatic method proposed in this work is an affordable option for precise determination of starch contents in several plant tissues.

Palavras-chave : hydrolases; starch; storage carbohydrates.

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