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MedicalExpress

Print version ISSN 2318-8111On-line version ISSN 2358-0429

Abstract

MORETTI, Isabele Fattori et al. The impact of interleukin-13 receptor expressions in cell migration of astrocytomas. MedicalExpress (São Paulo, online) [online]. 2015, vol.2, n.5, M150505. ISSN 2358-0429.  http://dx.doi.org/10.5935/MedicalExpress.2015.05.05.

INTRODUCTION:

Astrocytomas are common brain tumors. Increased expression levels of Interleukin-13 Receptor α2 (IL-13RA2) have been reported in astrocytomas. The Interleukin-13 signaling pathway may be associated with cell migration when binding to Interleukin-13 Receptor α1.

OBJECTIVE:

To investigate Interleukin-13 Receptor α1 (IL-13RA1) and IL13RA2 expression levels in human diffusely infiltrative astrocytomas and test the involvement of Interleukin-13 levels in cell migration in two glioblastoma cell lines.

METHODS:

IL13RA expression levels were accessed by quantitative real time PCR in 128 samples of astrocytomas and 18 samples of non-neoplastic brain tissues from temporal lobe epilepsy surgery. The impact of IL-13 levels (10 and 20 ng/mL) on cell migration was analyzed by the wound assay in U87MG and A172 cells.

RESULTS:

Glioblastoma presented higher IL13RA1 and IL13RA2 expression levels compared to lower grades astrocytomas and to non-neoplastic cases. U87MG and A172 cells presented higher expression levels of IL-13RA1 vs. IL-13RA2. A significant difference in migration rate was observed in A172 cells treated with 10 ng/mL of IL-13 vs. control: treated cells presented slower migration than non-treated cells. U87MG cells treated with IL-13 20ng/mL presented slower migration than non-treated cells. This indicates that the IL13Rα1 signaling pathway was not activated, indeed inhibited by the decoy IL-13Rα2, slowing cell migration. This impact occurred with a lesser concentration of IL-13 on the A172 than on the U87MG cell line, because A172 cells have a higher IL-13RA2/A1 ratio.

CONCLUSION:

The present results suggest IL-13 receptors as possible targets to decrease tumor cell migration.

Keywords : Astrocytoma; Interleukin-13; Interleukin-13 Receptors.

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