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Archives of Endocrinology and Metabolism

versão impressa ISSN 2359-3997versão On-line ISSN 2359-4292


MAGALHAES, Patrícia Künzle Ribeiro et al. Effects of drying and storage conditions on the stability of TSH in blood spots. Arch. Endocrinol. Metab. [online]. 2018, vol.62, n.2, pp.201-204.  Epub 05-Abr-2018. ISSN 2359-3997.


To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening.

Subjects and methods

Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage.


The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer.


Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account.

Palavras-chave : Neonatal screening; TSH; congenital hypothyroidism; filter paper storage.

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