Caracterização dos tipos celulares presentes na hemolinfa de larvas e ninfas de Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) em diferentes estados nutricionais

Carneiro, Maria Elisa; Daemon, Erik

doi:10.1590/S0101-81751996000300010

Resumo

With the purpose of characterize morphologically the hemocytes of larvae and nymphs of Rhipicephalus sanguineus on different nutritional phases, it was obtained samples of haemolymph were obtained by sectioning the forelegs and collecting the drop there formed. After dried, the samples were fixed by methanol and stained by Giemsa. Microscopical observation resulted in the characterization of five basic cellular types: prohemocytes, plasmatocytes, granulocytes, spherulocytes and oenocytoids. Moreover, undefined cell types, whose morphological patterns didn't have correlation with the characteristics cited for hemocytes, were found in low frequency. The change in the relative composition of the haemolimph was characterized by decrease of the number of granulocytes and greater variabilily of the cell types present in the hemolymph as the tick evolved. This fact may be linked to the alterations that these cells suffer along the development of the tick.

Ixodid; Rhipicephalus sanguineus; hemocytes; morphology

Caracterização dos tipos celulares presentes na hemolinfa de larvas e ninfas de Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) em diferentes estados nutricionais

Characterization of the celular types present in the Haemolymph of larvae and nimphs of Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) in different nutritional stages

Maria Elisa Carneiro; Erik Daemon

Departamento de Parasitologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro. Antiga Rodovia Rio-São Paulo Km 47,23851-970 Itaguaí, Rio de Janeiro, Brasil

ABSTRACT

With the purpose of characterize morphologically the hemocytes of larvae and nymphs of Rhipicephalus sanguineus on different nutritional phases, it was obtained samples of haemolymph were obtained by sectioning the forelegs and collecting the drop there formed. After dried, the samples were fixed by methanol and stained by Giemsa. Microscopical observation resulted in the characterization of five basic cellular types: prohemocytes, plasmatocytes, granulocytes, spherulocytes and oenocytoids. Moreover, undefined cell types, whose morphological patterns didn't have correlation with the characteristics cited for hemocytes, were found in low frequency. The change in the relative composition of the haemolimph was characterized by decrease of the number of granulocytes and greater variabilily of the cell types present in the hemolymph as the tick evolved. This fact may be linked to the alterations that these cells suffer along the development of the tick.

Key words: Ixodid, Rhipicephalus sanguineus, hemocytes, morphology

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Recebido em 26.II.1996; aceito em 07.XI.1996

Amosova, L.I. 1983. Ultrastructural characters of haemocytes of the ixodid tick Hyalomma asiaticum (Ixodidae). Parazitologiya 17: 126-133.
Arnold, J.W. 1970. Hamocytes of the pacific beetle cockroach, Diploptera punctata. Can. Entomol. 102: 830-835.
______. 1972. A comparative study of the haemocytes (blood cells) of cockroaches (Insecta: Dictyoptera: Blattaria), with a view of their significance in taxonomy. Can. Entomol. 104: 309-348.
______. 1974. The hemocytes of insects, p. 201-254. In: M. Rockstein (Ed.). The Physiology of Insecta. New York, Academic press, vol. 5.
Azambuja, P.; E.S. Garcia & N.A. Ratcliffe. 1991. Aspects of classification of hemiptera hemocytes from six triatomine species. Mem. Inst. Oswaldo Cruz 86: 1-10.
Brinton, L.P. & W. Burgdorfer. 1971. Fine structure of normal hemocytes in Dermacentor andersoni Stiles (Acari: Ixodidae). J. Parasitol. 57: 1110-1127.
Costa Leonardo, A.M. & C. Cruz-Landim. 1978. Estudo das células do sangue em Camponotus rufipes (Fabricius) Hymenoptera: Formicidae. Stud. Entomol. 20: 235-251.
Cruz-Landim, C. & M.A. Staurengo-Cunha. 1971. Transformações pós-embrionárias em Melipona quadrifasciata anthidioides Lep. V: Hemócitos das fases imaturas. Rev. Bras. Biol. 31: 471-483.
Dannhorn, D.R. & K.A. Seitz. 1987. Hemocytes of Leiobunum limbatum and two other species of harvestmen (Aracnida, Opiliones): Morphological classification and functional aspects. J. Morphol. 193: 185-196.
Dolp, R.M. 1970. Biochemical and physiological studies of certain ticks (Ixodoidea). Qualitative and quantitative studies of hemocytes. J. Med. Entomol. 7: 277-288.
Douglas, J.R. 1943. The internal anatomy of Dermacentor andersoni Stiles. Univ. Calif. Publ. Entomol. 7: 207-272.
el Shoura, S.M. 1986. Fine structure of the hemocytes and nephrocytes of Argas (Persicargas) arboreus (Ixodoidea: Argasidae). J. Morphol. 189: 17-24.
______. 1989. Ultrastructure of the larval haemocytes and nephrocytes in the tick Ornithodoros (Pavlovskyella) erraticus (Ixodoidea: Argasidae). J. Acarologia 30: 35-40.
Eggenberger, L.R.; W.J. Lamoreaux & L.S. Coons. 1990. Hemocytic encapsulation of implants in the tick Dermacentor variabilis. Exp. Appl. Acarol. 9: 279-287.
Giannotti, E. & F.H. Caetano. 1985. A comparative study of the hemocytes of Atta laevigata adults (Formicidae: Myrmicinae). Rev. Bras. Genet. 8: 37-45.
______. 1990. Caracterização morfológica dos hemócitos de Polistes lanio lanio (Hymenoptera, Vespidae) durante o desenvolvimento pós-embrionário. Rev. Bras. Biol. 51: 179-184.
Gupta, A.P. 1979. Hemocyte types: their structure, synonymies, interrelationships and taxonomic significance, p. 85-127. In: A.P. Gupta (Ed.). Insect Hemocytes. Cambridge, Cambridge University Press.
Jones, J.C. 1962. Current concepts concerning insect hemocytes. Am. Zool. 2: 209-246.
Kaaya, G.P. & N.A. Ratcliffe. 1982. Comparative study of hemocytes and associated cells of some medically important dipterans. J. Morphol. 173: 351-365.
Luckhart, S.; M.S. Cupp & E.W. Cupp. 1992. Morphological and functional classification of the hemocytes of adult female Simulium vittatum (Diptera: Simuliidae). J. Med. Entomol. 29: 457-466.
Neitz, W.O.; F. Boughton & H.S. Walters. 1971. Laboratory investigation on the life cycle of karoo paralysis tick (Ixodes rubicundus Neumann, 1904). Onderst. J. Vet. Res. 38: 215-224.
Nordenskiöld, E. 1905. Zur anatomie und histologie von Ixodes reduvius. Zool. Anz. 28: 478-485.
Ravindranath, M.H. 1974. The hemocytes of a scorpion Palamnaeus swammerdami. J. Morphol. 144: 1-10.
Sherman, R.G. 1973. Ultrastructurally different hemocytes in a spider. Can. J. Zool. 51: 1155-1159.
Tsileneva, V.A. 1959. Formed elements of the hemolymph of ixodid ticks. Dokl. Akad. Nauk. Tadzh. SSR 2 (1): 45-51.
Yeager, J.F. 1945. The blood picture of the southern armyworm (Prodenia eridania). J. Agric. Res. 71: 1-40.

Datas de Publicação

Publicação nesta coleção
13 Jul 2009
Data do Fascículo
1996

Histórico

Aceito
07 Nov 1996
Recebido
26 Fev 1996

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Amosova, L.I. 1983. Ultrastructural characters of haemocytes of the ixodid tick Hyalomma asiaticum (Ixodidae). Parazitologiya 17: 126-133.

[2] Arnold, J.W. 1970. Hamocytes of the pacific beetle cockroach, Diploptera punctata. Can. Entomol. 102: 830-835.

[3] ______. 1972. A comparative study of the haemocytes (blood cells) of cockroaches (Insecta: Dictyoptera: Blattaria), with a view of their significance in taxonomy. Can. Entomol. 104: 309-348.

[4] ______. 1974. The hemocytes of insects, p. 201-254. In: M. Rockstein (Ed.). The Physiology of Insecta. New York, Academic press, vol. 5.

[5] Azambuja, P.; E.S. Garcia & N.A. Ratcliffe. 1991. Aspects of classification of hemiptera hemocytes from six triatomine species. Mem. Inst. Oswaldo Cruz 86: 1-10.

[6] Brinton, L.P. & W. Burgdorfer. 1971. Fine structure of normal hemocytes in Dermacentor andersoni Stiles (Acari: Ixodidae). J. Parasitol. 57: 1110-1127.

[7] Costa Leonardo, A.M. & C. Cruz-Landim. 1978. Estudo das células do sangue em Camponotus rufipes (Fabricius) Hymenoptera: Formicidae. Stud. Entomol. 20: 235-251.

[8] Cruz-Landim, C. & M.A. Staurengo-Cunha. 1971. Transformações pós-embrionárias em Melipona quadrifasciata anthidioides Lep. V: Hemócitos das fases imaturas. Rev. Bras. Biol. 31: 471-483.

[9] Dannhorn, D.R. & K.A. Seitz. 1987. Hemocytes of Leiobunum limbatum and two other species of harvestmen (Aracnida, Opiliones): Morphological classification and functional aspects. J. Morphol. 193: 185-196.

[10] Dolp, R.M. 1970. Biochemical and physiological studies of certain ticks (Ixodoidea). Qualitative and quantitative studies of hemocytes. J. Med. Entomol. 7: 277-288.

[11] Douglas, J.R. 1943. The internal anatomy of Dermacentor andersoni Stiles. Univ. Calif. Publ. Entomol. 7: 207-272.

[12] el Shoura, S.M. 1986. Fine structure of the hemocytes and nephrocytes of Argas (Persicargas) arboreus (Ixodoidea: Argasidae). J. Morphol. 189: 17-24.

[13] ______. 1989. Ultrastructure of the larval haemocytes and nephrocytes in the tick Ornithodoros (Pavlovskyella) erraticus (Ixodoidea: Argasidae). J. Acarologia 30: 35-40.

[14] Eggenberger, L.R.; W.J. Lamoreaux & L.S. Coons. 1990. Hemocytic encapsulation of implants in the tick Dermacentor variabilis. Exp. Appl. Acarol. 9: 279-287.

[15] Giannotti, E. & F.H. Caetano. 1985. A comparative study of the hemocytes of Atta laevigata adults (Formicidae: Myrmicinae). Rev. Bras. Genet. 8: 37-45.

[16] ______. 1990. Caracterização morfológica dos hemócitos de Polistes lanio lanio (Hymenoptera, Vespidae) durante o desenvolvimento pós-embrionário. Rev. Bras. Biol. 51: 179-184.

[17] Gupta, A.P. 1979. Hemocyte types: their structure, synonymies, interrelationships and taxonomic significance, p. 85-127. In: A.P. Gupta (Ed.). Insect Hemocytes. Cambridge, Cambridge University Press.

[18] Jones, J.C. 1962. Current concepts concerning insect hemocytes. Am. Zool. 2: 209-246.

[19] Kaaya, G.P. & N.A. Ratcliffe. 1982. Comparative study of hemocytes and associated cells of some medically important dipterans. J. Morphol. 173: 351-365.

[20] Luckhart, S.; M.S. Cupp & E.W. Cupp. 1992. Morphological and functional classification of the hemocytes of adult female Simulium vittatum (Diptera: Simuliidae). J. Med. Entomol. 29: 457-466.

[21] Neitz, W.O.; F. Boughton & H.S. Walters. 1971. Laboratory investigation on the life cycle of karoo paralysis tick (Ixodes rubicundus Neumann, 1904). Onderst. J. Vet. Res. 38: 215-224.

[22] Nordenskiöld, E. 1905. Zur anatomie und histologie von Ixodes reduvius. Zool. Anz. 28: 478-485.

[23] Ravindranath, M.H. 1974. The hemocytes of a scorpion Palamnaeus swammerdami. J. Morphol. 144: 1-10.

[24] Sherman, R.G. 1973. Ultrastructurally different hemocytes in a spider. Can. J. Zool. 51: 1155-1159.

[25] Tsileneva, V.A. 1959. Formed elements of the hemolymph of ixodid ticks. Dokl. Akad. Nauk. Tadzh. SSR 2 (1): 45-51.

[26] Yeager, J.F. 1945. The blood picture of the southern armyworm (Prodenia eridania). J. Agric. Res. 71: 1-40.

Brasil

Brasil

Caracterização dos tipos celulares presentes na hemolinfa de larvas e ninfas de Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) em diferentes estados nutricionais

Characterization of the celular types present in the Haemolymph of larvae and nimphs of Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) in different nutritional stages

Resumo

Datas de Publicação

Histórico