Effect of ionizing radiation on rat parotid gland

Effect of ionizing radiation on rat parotid gland

George Boraks; Flávio Silva Tampelini; Kleber Fernando Pereira; Renato Paulo Chopard

Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil

^{Correspondence}

ABSTRACT

A common side effect of radiotherapy used in the treatment of oral cancer is the occurrence of structural and physiological alterations of the salivary glands due to exposure to ionizing radiation, as demonstrated by conditions such as decreased salivary flow. The present study evaluated ultrastructural alterations in the parotid glands of rats receiving a fractionated dose (1,500-cGy) of radiation emitted by a Cesium-137 source and rats that were not subjected to ionizing radiation. After sacrifice, the parotid glands were removed and examined by transmission electron microscopy. Damage such as cytoplasmic vacuolization, dilatation of the endoplasmic reticulum and destruction of mitochondria, as well as damage to the cellular membrane of acinar cells, were observed. These findings lead to the conclusion that ionizing radiation promotes alterations in the glandular parenchyma, and that these alterations are directly related to the dose level of absorbed radiation. Certain phenomena that appear in the cytoplasm and nuclear material indicate that ionizing radiation causes acinar cell death (apoptosis).

Key Words: irradiation, parotid gland, transmission electron microscopy.

RESUMO

Um efeito colateral comum da radioterapia usada no tratamento de câncer na cavidade oral é a ocorrência de alterações estruturais e fisiológicas sobre as glândulas salivares por exposição à radiação ionizante, como demonstrada em situações com decréscimo do fluxo salivar. O presente estudo teve por objetivo avaliar as alterações ultra-estruturais de glândulas parótidas de ratos que receberam uma dose fracionada (1500 - cGy) de radiação emitida por uma fonte de Césio 137 e ratos que não receberam a radiação ionizante. Após o sacrifício, as glândulas parótidas foram removidas e examinadas por microscopia eletrônica de transmissão. Lesões das organelas citoplasmáticas, como dilatação do retículo endoplasmático, destruição das mitocôndrias e formação das vacuolizações citoplasmáticas, além de lesão da membrana celular das células acinares foram observadas. Portanto, a radiação ionizante promove alterações no parênquima glandular, o que está diretamente relacionado com a dose de radiação absorvida. Determinados fenômenos que surgem no citoplasma e material nuclear são indicadores de que a radiação ionizante leva a célula acinar a morte programada (apoptose).

INTRODUCTION

The use of ionizing radiation as a therapeutic measure leads to significant morphofunctional alterations in the stomatognathic system, in both hard and soft tissues (1,2). Ionizing radiation can produce detrimental changes in several classes of macromolecules found in cells.

In the case of proteins, the damage is the loss of lateral chains and alterations in secondary and tertiary structures, resulting in non-conformity of the protein molecules. In lipids, exposure to such radiation stimulates peroxidation of unsaturated fatty acids. Changes to nucleic acids may include damage to the base, base loss and breaks in one or both filaments of the DNA double helix. The effects of ionizing radiation are directly related not only to the type of the tissue receiving the radiation, but also to the absorbed radiation dose. Doses higher than 1000 cGy have been shown to promote alterations in the glandular parenchyma of irradiated rats as early as 6 h after irradiation (3).

The aim of this study was to evaluate the ultrastructural alterations that occur in parotid gland acinar cells of Wistar rats submitted to ionizing radiation.

MATERIAL AND METHODS

Ten adult male Wistar rats (Rattus novergicus) weighing between 250 and 350 g were allocated to 2 groups. Animals in GI (control; n=5) were not subjected to ionizing radiation, whereas those in GII (experimental; n=5) received a dose of 1,500-cGy fractionated in 750 cGy/week. All animals were housed in a temperature-controlled environment (21-23ºC), maintained on a 12-h light/12-h dark cycle and given free access to food and water.

All animals were anesthetized with an intramuscular injection of a combination of ketamine hydrochloride (Ketalar^®; Parke Davis, Morris Plains, NJ, USA; 35 mg/kg), xylazine hydrochloride (Rompum^®; Bayer do Brasil S/A, São Paulo, SP, Brazil; 5.0 mg/kg) and acepromazine (Acepran^®; Univet, São Paulo, SP, Brazil; 0.75 mg/kg). Subsequently, study animals were exposed to gamma radiation emitted from a Cesium-137 radioactive source positioned at a distance of 20 cm from skin surface. A total dose of 1,500-cGy was used fractionated in 750 cGy/week. During the procedure, the animals received radioprotection by means of a rectangular lead shelter (30-cm long, 20-cm deep and 5-cm thick), leaving only the head and neck exposed and allowing approximately 3% of the total radiation dose to be directed to that region.

Immediately after exposure to the second fractioned dose of radiation, study and control animals were sacrificed by ketamine overdose and had the parotid glands removed. In preparation for examination by transmission electron microscopy (TEM), the glands were fixed in glutaraldehyde solution (2.5%), fixed again in uranyl acetate (0.5%), dehydrated, embedded in resin, cut into semi-thin and ultra-thin longitudinal sections, and examined with a transmission electron microscope (JEOL 1010; Jeol, Tokyo, Japan).

RESULTS

Figures 1A-^C and ^2A-^C present TEM micrographs of GI and GII animals, respectively.

In the ultrastructural analysis, the acinar cells of the parotid glands from GII animals showed various alterations in the cytoplasmic organelles. Similarly, the granular endoplasmic reticula in the parotid glands from GI animals were rectangular and dense (Fig. 1A), whereas those in GII samples presented some expanded portions (Fig. 2B).

The mitochondrias in GI samples were of various sizes and morphology, but maintained the parallel aspect of the outer membrane, and dense areas of chromatin was observed at the periphery of the nucleus (Fig. 1B). In GII samples, in addition to intramitochondrial vacuolization, there was complete disruption of the outer mitochondrial membrane, suggesting degeneration of the organelle (^{Fig. 2C}).

In the acinar cells of GII animals, widely dispersed cytoplasmic vacuolization was observed. In some cases, this vacuolization displaced the nucleus from its original position (^{Fig. 2A}).

Intercellular unions, characterized by desmosomes and interdigitation, were also evaluated. In GI samples, the integrity of the desmosomes was preserved (^{Fig. 1C}), in the same way as it was in samples from GII animals (^{Fig. 2C}).

DISCUSSION

This study evaluated comparatively the effects resulting from exposing Wistar rats to a radiation dose of 1,500 cGy, fractionated to 750 cGy/week.

The cytoplasmic vacuolization seen in samples from irradiated animals confirms data from previous studies (4-8). Some authors have observed that the expansion of the endoplasmic reticulum is associated to the cellular status that precedes apoptosis (4,9-15). In accordance with these findings, areas of circular or concentric expansion in the endoplasmic reticula of irradiated animals were observed in this study. In general, this expansion is related to compression of the nuclear material. These widely dispersed intranuclear alterations may be attributed to the effect of the ionizing radiation. This explains the decreased expression of PCNA in the nuclei of irradiated acinar cells, as found by Takahashi et al. (12).

The cell membrane also presented signs of disruption. Based on the findings of Sesso et al. (14), this disruption is characteristic of the cellular stage that precedes apoptosis, since the loss of the outer nuclear membrane occurs right before apoptosis.

In samples from irradiated animals (GII), there was a disruption of the internal mitochondrial membrane and presence of vacuolization, as well as displacement of the outer mitochondrial membrane. Electron-dense granules were also observed in the mitochondrial interior, representing the effective loss of integrity of the mitochondrial membrane and leading to degeneration of the organelle and cell death. These findings support those from previous studies (5,10,11,14,16-19).

Based on the employed methodology and the obtained results, it may be concluded that 1,500-cGy fractionated doses of ionizing radiation significantly altered acinar cells in rat parotid glands, as well as the serous acinar cells found at the interface, eventually leading to apoptosis.

Accepted July 2, 2004

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Publication Dates

• Publication in this collection
  22 Apr 2008
• Date of issue
  2008

History

• Accepted
  02 July 2004
• Received
  02 July 2004