Determination of the chemical composition, amino acid levels and energy values of different poultry offal meals for broilers

Determination of the chemical composition, amino acid levels and energy values of different poultry offal meals for broilers

Silva EP da^I; Rabello CBV^I; Lima MB de^I; Arruda EMF de^I; Ludke JV^II; Ludke MCMM^I

^IUniversidade Federal Rural de Pernambuco, Brazil, Dois Irmãos, CEP: 51.191-900, Recife, Pernambuco, Brazil, E-mail: [e-mail]

^IIEMBRAPA Suínos e Aves

ABSTRACT

The aim of this study was to determine the chemical composition, amino acid content and energy values, as well as to quantify the passage rate in the digestive tract of three different poultry offal meals (POM), in two experiments carried out with broilers. In the first experiment, metabolizable energy values were determined (AMEn and TMEn) using the method of total excreta collection. In this experiment, 150 15-d-old male and female broilers were distributed according to a completely randomized experimental design with 5 treatments of 5 replicates of 6 birds each. The following treatments were applied: a reference diet, three test diets consisting of 250 g/kg of the tested ingredient (POM) + 750 g/kg of the reference diet, and one group of birds fasted for excreta collection to determine endogenous and metabolic losses. In the second experiment, POM passage rate results were obtained using 90 26-d-old broilers distributed according a completely randomized design with three treatments of 5 replicates of 6 birds each. POM passage rate was correlated (r = -99.86%) with its EE content. The evaluated POMs presented the following ME values: 16,727; 15,781 and 17,443 MJ AMEn /kg and 17,877; 15,882 and 17,527 MJ TMEn /kg for samples A, B and C, respectively.

Keywords: Alternative feedstuffs, poultry by-products, passage rate.

INTRODUCTION

Feed formulas based on traditional feedstuffs are becoming increasingly expensive because the prices of the commonly used conventional sources of protein (soybean meal) and the phosphorus (dicalcium phosphate) are becoming incompatible with chicken prices. For this reason, the use of animal ingredients may be an alternative for the nutritionists to formulate more economical feeds, despite of restrictions of the foreign markets.

In the specific case of poultry offal meals, the different ratios of the parts included in the meal, such as head, feet, back, and other rejected parts derived from slaughter and deboning may result in significant variations in their composition, and consequently, in the utilization in their energy content.

The success of the formulation depends on the adequate supply of animal nutritional requirements, which requires constant updating of the nutritional matrix with the composition of the feedstuffs that will be included in feed formulation. However, it is not always possible to obtain this information, making nutritionist resort to the composition of feedstuffs published in recommendation tables, which not always accurately reflect the chemical and energy composition of each ingredient.

This situation is shown in the study of Nascimento et al. (2002). The authors evaluated five different poultry offal meals and determined a difference of 3,939 MJ/kg metabolizable energy between the evaluated meals, with ranges between 11,859 and 15,798 MJ/kg.

Differences in the proportion of carcass parts included and in the manufacturing process result in different compositions, particularly relative to their metabolizable energy values. So, when using feedstuff composition tables for feed formulation, dietary energy values may be under- or overestimated, affecting feed calorie content, as well as the efficiency and the cost of energy intake per weight gain.

The objective of the present study was to evaluate the chemical composition and amino acid values, to estimate gut passage rate, and the energy values of different poultry offal meal in an experiment carried out with broilers.

MATERIALS AND METHODS

The experiment was carried out at the Department of Animal Science of Universidade Federal Rural de Pernambuco, Brazil. Three different poultry offal meals were obtained from three different processing plants in the state of Pernambuco, Brazil. Samples from each meal were collected and submitted to the Animal Nutrition Laboratory (LAN) of the Department of Animal Science of that university, and analyzed for moisture, dry matter, crude protein, ether extract, gross energy, ashes, calcium, phosphorus, sodium, chlorine, and potassium contents, as well as for density and geometric mean diameter. Samples of about 100 g of each meal were collected, duly identified, and analyzed for their essential amino acid contents.

The chemical analyses were carried out at the Animal Nutrition Laboratory (LAN) of the Department of Animal Science of the Universidade Federal Rural de Pernambuco, except for gross energy, calcium, phosphorus, sodium, chlorine and potassium analyses. Minerals were prepared at LAN and sample reading and standard curve determination were performed by flame photometry and atomic absorption at the Soil Fertility and Chemistry laboratory of that university.

Feedstuff, feed, and excreta gross energy was determined in a calorimetric bomb (IKA WERKE, model 2000) at the Animal Nutrition Laboratory of the Department of Animal Science Nutrition of Universidade Federal Rural do Semiárido. Feedstuff amino acid contentes were determined by near-infrared reflectance (NIR) by the company Degussa.

Chemical analyses were carried out using the methodologies described by Silva & Queiroz (2002). The geometric mean diameter (GMD) of each meal was determined according the procedure described by Zanotto & Bellaver (1996), using a vibrator assembly consisting of the device VIATEST 76773 KUHARDT and seven sieves (4, 2, 1.20, 0.60, 0.30, 0.15, and 0 mm).

A metabolic trial was carried out, using the method of total excreta collection, to determine the apparent and true coefficients of dry matter metabolizability (DMMC – A and DMMC – T, respectively), apparent and true coefficients of gross energy metabolizability (GEMC - A and GEMC – T, respectively), and apparent metabolizable energy (AME), apparent metabolizable energy corrected for nitrogen balance (AMEn), true metabolizable energy (TME) and true metabolizable energy corrected for nitrogen balance (TMEn) values of the three poultry offal meal for broilers.

A flock of 540 straight-run broiler chicks were reared on floor pens, and were offered a diet based on corn and soybean meal formulated to supply their nutritional requirements. Birds were then selected by weight in order to obtain maximal uniformity during the experimental period. A number of 150 straight run Cobb broilers, with initial age of 15 days, were used in the metabolism assay carried out at the Non-Ruminant Digestibility Laboratory. These birds were housed in battery cages measuring 1.00 x 0.50 x 0.50 m in an environmentally-controlled room. A lighting program of 24 hours of light (natural + artificial) was adopted. During the experimental period, feed intake and excretion data were recorded. Environmental temperature was maintained at 24 ºC ± 1.5 ºC.

Birds were distributed according to a a completely randomized experimental design with five treatments of five replicates of six birds each, being three males and three females. The following treatments were applied: a reference diet (T1) based on corn and soybean meal (Table 1) and three test diets (T2, T3, T4) consisting of 250 g/kg of the tested ingredient + 750 g/kg of the reference diet (T1). The fifth treatment (T5) consisted of a group of birds fasted to quantify fecal metabolic dry matter + endogenous urinary dry matter, fecal metabolic nitrogen + endogenous urinary nitrogen, and fecal metabolic energy + endogenous urinary energy in order to estimate true metabolizability of dry matter and of energy of the tested feedstuffs.

The diets offered during the rearing phase and during the experimental period were formulated based on feedstuff composition and on the specific nutritional requirements of broilers for each rearing phase, as recommended by Rostagno et al. (2005).

Under the floor of the metabolic cages (experimental units), trays covered with canvas were place for total excreta collection to determine metabolic and endogenous losses. The first five days were used for bird adaptation to the cages and to the experimental diets, and the next five days for total excreta collection. Endogenous and metabolic losses were determined after a period of 24 hours of fasting for cleaning of total digestive tract, and then 48 hours for the collection of the total material excreted. The calculation of DMMC – V, GEMC – V, TME, and TMEn considered the endogenous and metabolic losses corrected for five experimental days. During the experiment, excreta and endogenous and metabolic losses were collected twice daily with an interval of 12 hours between collections. The collected material was placed in duly identified bags, weighed in a 1-g precision scale, subtracting bag weight, and subsequently frozen at -20 ºC.

The mash diets were offered ad libitum during the entire experimental period. Ferric oxide in powder was added at a level of 10g/kg to the experimental diets as fecal marker in the beginning and end of the period of total excreta collection. At the end of the experiment, excreta and endogenous and metabolic losses were thawed and homogenized per experimental unit. An aliquot of 500 g of excreta was sampled, whereas for endogenous and metabolic losses, all the collected material was used.

Samples were pre-dried in a forced-ventilation oven at 55 ºC for 72 hours. Samples were then ground in grinder with 1-mm sieve and analyzed for dry matter, nitrogen, and gross energy contents, according to the previously described methods.

The coefficients of DMMC – A, DMMC – V, GEMC – A, GEMC – V and AME, AMEn, TME and TMEn values of the three tested poultry offal meals were calculated using the formulas proposed by Matterson et al. (1965). Considering the gross energy (GE) and AMEn values of the feedstuffs, the coefficient of gross energy metabolizability (GEMC) was calculated according the equation of the ARC (1980).

In order to determine feed passage rate, 90 Cobb broilers (45 males and 45 females), with an initial age of 26 days, were distributed according to a completely randomized experimental design into three treatments of five replicates of six birds per experimental unit, with three males and three females each. Treatments were based on the feeding of the pure tested feedstuffs, i.e., the poultry offal meals obtained from the three different processing plants: Processing Plant A (T1), Processing Plant B (T2) and Processing Plant C (T3).

Birds were fed ad libitum only the tested poultry offal meal for a limited time. In order to facilitate excreta separation and collection, 1% of a blue dye was added to the total amount of feed in the feeder. At the end of 12 hours, precisely, of ad libitum feeding, residues were removed, and the same feedstuff was offered, but with the addition of a red dye (ferric oxide) used as marker. The first collection was made six hours after the first marked excreta appeared, and the second collection after 12 hours of intake, as determined by the excretion of red-marked excreta. Average temperature during the experiment was 25 ºC.

The total amount of excreta was weighed and placed in plastic bags identified per experimental unit and frozen at -22 ºC. Excreta samples were prepared as described above. Only dry matter content was determined. Passage rate was interpreted using the information on dry matter excreted per hour (DM/h), was calculated according to the following equation:

where; % ADS = air-dried sample at 55 ºC for 72 h, and % ODS = oven-dried sample at 105 ºC for 16 h.

The coefficients of metabolizability and feedstuff passage rate were submitted to analysis of variance, and when differences were detected by the F test, the test of Student-Newman-Keuls was applied, at 5% probability level. Feedstuff passage rates were tested by Pearson's parametric correlations at 5% significance level. Statistical analyses were carried out by the statistical package SAEG – UFV, version 9.1 (2006).

RESULTS

Table 2 shows the chemical composition results of the three tested poultry offal meals. Crude protein, ether extract, gross energy and mineral (calcium, phosphorus, sodium, chlorine and potassium) contents widely varied among meals. It was determined that sodium, chlorine and potassium content were negatively correlated with ash content (Table 3).

Density varied between 425 and 533 g/L according to ether extract content. These two parameters presented a highly significant correlation of 0.999 (p<0.001).

More than 90% of the particles of meals A and B were classified as medium (54%) and fine (42%), whereas medium particles (67%) predominated in meal C, where the remaining 35% consisted of fine (19%) and coarse (17%) particles.

Table 4 presents amino acid contents and the correlation coefficients with crude protein of the three poultry offal meals. The analyzed amino acids presented high correlation with crude protein content, except for cystine, which correlation value was low and not significant.

Metabolism assay

The gastrointestinal passage rates of the tested poultry offal meals and their correlation with fat content of the respective meals are shown Table 5. The results demonstrate that meal C presented the lowest dry matter excretion per hour, i.e., the slowest passage rate, and therefore, it remained longer in the gut, followed by meal A, and whereas meal B presented the fastest passage rate.

The AME, AMEn, TME and TMEn values presented in Table 6 show the differences among the evaluated meals.

The apparent and true coefficients of metabolizability of dry matter and gross energy are in Table 7. The differences determined are negligible when the standard deviations of the means are considered.

The results of the F test revealed that the apparent metabolizability of dry matter was not different from the true metabolizability (Table 7).

The ratio between ashes and ether extract influenced the metabolizable energy values of the meals, and the following ratios between ashes and ether extract found were: 0.66; 0.82 and 0.49, for meals A, B and C, and metabolizable energy values were 16,723, 15,781 and 17,439 MJ/kg, respectively. These ratios imply that the high fat content in meals will not necessarily mean that their energy will be well utilized.

DISCUSSION

Dry matter and moisture contents may indicate over- or under-heating of meals during processing. Bellaver & Zanotto (2004) mentioned that the desirable moisture levels are between 40 and 100g/kg, and therefore, the three tested poultry offal meals are within this optimal range, suggesting that processing was adequate, taking only moisture into consideration.

The predominance of determined body parts used when manufacturing poultry meals have a strong influence of their crude protein, ether extract, and ashes content. Analyzing 22 poultry offal meals, Dale et al. (1993) found mean values of 478, 296 and 66 g/kg for those parameters respectively. The significant differences detected among the evaluated meals in ether extract and ashes contents may be attributed to the proportions of raw materials used during manufacturing, particularly in the proportions of head, back, and feet.

Literature information (Nascimento et al., 2002; Paula et al., 2002; Tucci et al., 2003; D'Agostini et al., 2004; Nunes et al., 2005; Nery, 2005; Brumano et al., 2006a; Nunes et al., 2006; Melo, 2007; Ramalho, 2008) on the composition of poultry offal meals produced in Brazil show that 47% of the meals presented crude protein (CP) contents between 577 and 614 g/kg, and ashes between 118 and 162 g/kg, and that about 290 g/kg of the samples contained between 101 and 161 g/kg of ether extract (EE). However, the 53, 36 and 71% CP, ashes and EE contents obtained in the tested meals were not within that range, confirming the heterogeneity of processing methods, particularly relative to fat content.

The variation observed in EE content may be related to how digesters are fed, as well as to how meals are fried. Offal may be transported to the digester by gravity with the use of water, by augers or by belts. The two last methods directly feed the digester, maintaining the integrity of the raw materials, which are still raw. The water used to transport the offal by gravity is separated using a sieve. Part of the water returns to the feather-plucking area, and aids transporting the feathers. However, this water also transports part of the visceral fat, which binds to the feathers during transport (Maffi, 1993); therefore, the chemical components of the poultry offal meal are not fully utilized. According to Maffi (1993), this practice in rendering plants allows saving water in the system.

Another explanation of the high variability in the fat content of poultry offal meals may be located to where the raw material is cooked in the digester. In order to improve this process, some rendering plants add to the mass approximately 35.7 g/kg of fat (Sabino & Finzer, 2006), and consequently this fat is added to that present in the raw material, influencing poultry offal meal fat content.

The average levels of calcium and phosphorus of the tested poultry offal meals were different from those reported in some research studies (Nascimento et al., 2002; Soares et al., 2005; Brumano et al., 2006a; Nunes et al., 2006), which presentd mean values of 37.1 and 27.3, respectively, and a calcium:phosphorus ratio of 1.36:1. Calcium and phosphorus levels obtained in the present study were also different from those in the ten poultry offal meals analyzed by Najafabadi et al. (2007), but the calcium:phosphorus ratio of 1.8:1 obtained here was consistent with that study. The variation in the proportion of parts that compose the residue sent to the rendering plant may justify these results, when considering carcass processing and trading. Some companies debone breasts and legs, and therefore breast cartilage and leg bones are included in the residue commonly sent to the rendering plant (Roque, 1996), contributing to the variation in the levels of calcium and phosphorus in poultry offal meals. The technological level of the processing plants may also influence those levels relative to the recovery of meat adhered to the parts, particularly in the breast and neck (Roque, 1996), that is, a better recovery of meat increases the proportion of bones in the meal, and consequently, increases its calcium and phosphorus content.

There are few studies in literature relative to sodium, chlorine and potassium levels in poultry offal meals. In a sample of poultry offal meal with 246.3 g/kg of ashes, analyzed by Nunes et al. (2005), levels of 6.2 g/kg of sodium and 4.8 g/kg potassium were determined. The 13 poultry offal meals evaluated by Robbins & Firman (2006), with ashes contents between 10 and 28g/kg, sodium and potassium values of 5.6 and 6.8 g/kg, respectively, were found. The authors found that the levels of those minerals were negatively correlated with ash content, with the highest Na value (8.8g/kg) obtained from a meal with 100 g ashes /kg, and the lowest K concentration in the meal containing 280 g ashes /kg. Najafabadi et al. (2007) analyzed 10 poultry offal meals and obtained average values of ashes (93.4g/kg), sodium (5.2 g/kg), potassium (3.1 g/kg) and chlorine (7.4 g/kg) which varied 22.2, 11.5, 16.1, and 9.5%, respectively among meals. To date, literature does not provide clear explanations for these variations.

The significant negative correlation between these three minerals and ashes content found in the present study may be used as a tool for nutritionists to correct the variations in sodium, chlorine and potassium contents, using the ratios of 45.6, 57.4 and 74.4 g/kg ashes, respectively.

In summary, differences in the chemical composition of poultry offal meals are a result of the criteria and methods applied in the processing plants as well as for processing this by-product in the rendering plants. These differences are very common, because, according to Lopez (2005), there is no legal standardization of the chemical composition of poultry offal meal in Brazil, and the composition is usually determined by the feed manufacturing companies.

Information in raw material density is important and should be considered when designing feeding strategies, particularly when least cost formulations are used. The density of alternative feedstuffs should be similar to those of corn (724 - 757 g/L) and soybean meal (639 - 675 g/L), according to Rodrigues et al. (2001) and Rodrigues et al. (2002). It is well known that, among factors regulating feed intake, physical limitations, i.e., the distension of the gut muscles, are imposed when high levels of light feedstuffs are included in the feed, because the stomach is quickly filled (Gonzales et al., 2002). Poultry offal meals, however, present higher energy density than soybean meal, as shown by the density and metabolizable energy values obtained bt Rodrigues et al. (2002). Comparatively, at the same energy supply, one cubic centimeter of poultry offal meal corresponds to 1.6 cm³ of soybean meal, which may attenuate the gut-filling effects caused by the low density of poultry offal meals.

Particle geometric mean diameter is correlated to the development of the gizzard muscles (Ribeiro et al., 2002) and to digesta passage rate in the digestive tract (Nir et al., 1994). The average geometric mean diameter obtained in the present study was close to the value of 619 µm recommended by Ribeiro et al. (2002) to improve dietary energy metabolizability.

The determination of the amino acid composition of feedstuffs is currently required in feed formulation to precisely supply the amino acid requirements of animals. This is particularly important for poultry offal meal, which may present widely different protein levels. In addition of the absolute values, the ratio among amino acids must also be considered. In general, amino acid absolute values varied according to the crude protein content of the meals, except for sulfur amino acids, particularly cystine. The low correlation coefficient between crude protein and this amino acid may be attributed to the different cooking times and temperatures used for processing methods of poultry offal meals. According to Moritz & Latshaw (2001) cystine can be considered heat sensitive. Therefore, it may be inferred that poultry offal meals B and C may have been submitted to conditions that compromised cystine stability. Leucine, lysine and valine presented the widest variation among amino acids in the tested poultry offal meals, which was also observed by Nascimento (2000) and Brumano et al. (2006b). The ratio among valine, leucine and isoleucine has been discussed in literature, as they present specificity for the membrane transport system and use the same enzymes for this end (Peganova & Eder, 2003). However, in practical diets, these amino acids are not included to correct possible unbalances, reinforcing the need of having detailed information of the ratio among these amino acids in the feedstuffs.

According to Swenson & Reece (1996), longer permanence times of feed in the gut allow better enzymatic action on the macromolecules and, consequently, higher release of monomers on the surface of the brush border of the enterocytes, and this is considered one of the extra-caloric effects of dietary fats. This phenomenon is allowed by action of fats, which stimulate the secretion of cholecystokinin (CCK) and motilin, according to Martínez et al. (1993;1995) and Rodríhuez-Sinovas et al. (1997). However, this effect is not constant, as there are reports that high fat content, together with high ashes content, may result in high fatty acid and calcium levels in the digesta, causing successive saponification reactions with the formation of insoluble soaps during digestion, with consequent reduction of absorption, particularly of unsaturated fatty acids (Lessire et al., 1985; Pesti et al., 1986; Martosiswoyo & Jensen, 1988). Therefore, ashes to ether extract ratios (A/EE) higher or equal to 1.0 may reduce the energy content of poultry offal meals, according to Nunes et al. (2006), who evaluated poultry offal meals with 1.17 and 1.02 A/EE ratios and observed final energy values of 11.512 and 12.847 MJ/kg, respectively.

Metabolism assay

Considering the influence of ashes to ether extract ratios on the metabolizable energy values of poultry offal meals, 0.66, 0.82 and 0.49 ratios and metabolizable energy values of 16.723, 15.781 and 17.439 MJ/kg for meals meal A, B and C, respectively, were obtained in the present study. These ratios indicate that the energy utilization of meals with high fat content will not necessarily be adequate.

The energy values of three tested poultry offal meals were higher than those determined by Brumano et al. (2006), who obtained 59% gross energy metabolizability, corresponding to 12.516 MJ/kg apparent metabolizable energy corrected for nitrogen (AMEn) for a poultry offal meal with high fat content (200g/kg EE). On the other hand, the calcium level of that meal was, in average, 60% higher than the poultry offal meals tested in the present experiment.

Other factors may also influence energy utilization in broilers, including primarily feedstuff quality and secondly the methodology applied for energy determination, replacement levels, and Bird age at the time of collection. These are only the factors affecting the method of total excreta collection.

As to feedstuff quality, Racanicci et al. (2004) stressed that the fatty acid profile of the fat in poultry offal meals influences the development of lipid oxidation. According to those authors, for each gram of oxidized fat in the feedstuff, 17% fat utilization is lost compared with a gram of non-oxidized fat.

The effects of the method of determination on metabolizable energy values were studied by Pesti et al. (1986). The methods of Matterson et al. (1965), Hill & Anderson (1958) and Sibbald (1976) were tested by Pesti et al. (1986), when evaluating poultry offal meals, and the values of 12,516, 11,449 and 16.409 MJ/kg were obtained for the respective methods, thereby proving those effects.

The determination of apparent metabolizable energy corrected for nitrogen in broilers proposed by Rostagno et al. (2005) uses the method of total excreta collection. The value presented by those authors for high-fat poultry offal meal was lower than those obtained for the tested poultry offal meals A, B, and C in the present experiment of 0.368, 1.310 and 2.026 MJ/kg, respectively. These results substantiate the hypothesis of Nunes et al. (2001), who stressed the importance of developing studies to improve the recommendation tables, making them more complete and precise.

The level of feedstuff replacement used in that method for the determination of metabolizable energy in the tested poultry offal meals was questioned by the authors. The energy values were obtained using 25% poultry offal meal; for this level, according to the equations of Paula et al. (2002) and Nascimento et al. (2005), AMEn values were 15,019 and 12,688 MJ/kg, respectively. Despite the different calculated values, these differences may be justified by the chemical composition of the feedstuffs, and there is consensus among authors that increasing inclusion levels reduce the metabolizable energy values of poultry offal meals.

The effect of bird age during the period of collection is also important. The positive visceral allometric growth in broiler chicks results in the development of the digestive tract and in an increase in enzyme secretion and absorption area (Sklan, 2001). These physiological event affect feed passage rate and, consequently, metabolizable energy values. Nascimento et al. (2005) determined poultry offal meal AMEn at two ages (16 - 23 and 30 - 38 days of age) and found a 0.435 MJ/kg increase in metabolizability. Similarly, Brumano et al. (2006a), using broilers with average ages of 25 and 45 days, found AMEn values of 12,516 and 13,278 MJ/kg for poultry offal meal, confirming the additive effect of age on energy utilization. However, these values are lower than those obtained in the present study, stressing the importance of standardizing the chemical composition of poultry offal meals, preserving their quality during processing and storage, and showing that low ratios between ashes and fat should be avoided.

According to Oliveira (1996), the heat treatment used during poultry offal meal processing may eliminate Salmonella present in the viscera, but Nunes et al. (2006) stress that the temperature applied may break the bonds among amino acids that maintain the tridimensional structure of protein, as well as promoting the formation of complexes among nutrients, rendering them indigestible and, as their absorption is reduced, the gross energy metabolizability of the feedstuff may be affected. The magnitude of these effects varies according to the processing methodology employed by the rendering companies (Albino et al., 1982; Albino et al., 1986). This means that fecal metabolic and endogenous urinary dry matter losses had little influence on the amounts excreted by the birds. This also applies to gross energy metabolizability, as GEMC-T considers fecal metabolic and endogenous urinary dry matter, fecal metabolic and endogenous urinary nitrogen, and fecal metabolic and endogenous urinary energy. In terms of methodology, feed intake has an important influence on endogenous and metabolic losses in metabolism assays (Silva et al., 2006). Coelho et al. (1983) and Lima et al. (1989) reported that, at normal intake levels, endogenous and metabolic losses are small relative to the excretion of energy derived from feed and have little influence on metabolizable energy values.

CONCLUSIONS

The tested poultry offal meals presented variable physical-chemical composition, leading to changes in passage rate and in energy utilization, particularly due to their fat and mineral contents.

ACKNOWLEDGEMENTS

The authors thank the companies Mauricéa Alimentos do Nordeste Ltda, Notaro Alimentos and Agropecuária Serrote Redondo for donating the poultry offal meals, as well as the Soil Fertility and Chemistry Laboratory of Universidade Federal Rural de Pernambuco (UFRPE), Animal Nutrition Laboratory of Universidade Federal Rural do Semi-árido (UFERSA) and the company empresa DEGUSSA, which kindly performed the required analysis.

Submitted: April/2011

Approved: November/2011

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Publication Dates

• Publication in this collection
  15 Aug 2012
• Date of issue
  June 2012

History

• Received
  Apr 2011
• Accepted
  Nov 2011