Estudo dos linfócitos circulantes por anticorpos monoclonais na miastenia grave

Marchiori, Paulo E.; Duarte, Alberto J.; Birolli, M. Izabel A. M.; Figueiredo, Cristina A.; Scaff, Milberto; Assis, J. Lamartine de

doi:10.1590/S0004-282X1988000300003

Resumos

Os autores avaliam os linfócitos T (CD3, CD4, CD8, CD4/8) por anticorpos monoclonais e rosácea em 20 pacientes e linfócitos B por Fab' por imunofluorescência em 9 pacientes com miastenia grave. Observam elevação significante na população de linfócito B e redução nos linfócitos T totais CD3+ por rosáceas. Não foram observadas modificações nas subpopulações celulares com timectomia e corticosteróides.

A significant decline of CD3 cell detected by rosettes and a significant increased of B cell populations were observed. The total CD3+, helper CD4+ and suppressor CD8+ T-cell subsets showed no significant variation em relation to sex, age thymectomy and corticotherapy by monoclonal antibodies.

Paulo E. Marchiori^I; Alberto J. Duarte^II; M. Izabel A. M. Birolli^III; Cristina A. Figueiredo^III; Milberto Scaff^IV; J. Lamartine de Assis^IV

^IMédico Assistente da DNC - Disciplina de Neurologia Clínica (DNC) do Departamento de Neurologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Laboratório de Imunogenética e Transplante Experimental (LIM 73) do Instituto dos Laboratórios de Investigação Médica (LIM) do Hospital das Clínicas (HC) da FMUSP

^IIProfessor Livre Docente da Disciplina de Alergia e Imunologia da FMUSP - Disciplina de Neurologia Clínica (DNC) do Departamento de Neurologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Laboratório de Imunogenética e Transplante Experimental (LIM 73) do Instituto dos Laboratórios de Investigação Médica (LIM) do Hospital das Clínicas (HC) da FMUSP

^IIIBiologista do LIM 73 - Disciplina de Neurologia Clínica (DNC) do Departamento de Neurologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Laboratório de Imunogenética e Transplante Experimental (LIM 73) do Instituto dos Laboratórios de Investigação Médica (LIM) do Hospital das Clínicas (HC) da FMUSP

^IVProfessor Adjunto da DNC - Disciplina de Neurologia Clínica (DNC) do Departamento de Neurologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Laboratório de Imunogenética e Transplante Experimental (LIM 73) do Instituto dos Laboratórios de Investigação Médica (LIM) do Hospital das Clínicas (HC) da FMUSP

RESUMO

Os autores avaliam os linfócitos T (CD3, CD4, CD8, CD4/8) por anticorpos monoclonais e rosácea em 20 pacientes e linfócitos B por Fab' por imunofluorescência em 9 pacientes com miastenia grave. Observam elevação significante na população de linfócito B e redução nos linfócitos T totais CD3+ por rosáceas. Não foram observadas modificações nas subpopulações celulares com timectomia e corticosteróides.

SUMMARY

A significant decline of CD3 cell detected by rosettes and a significant increased of B cell populations were observed. The total CD3+, helper CD4+ and suppressor CD8+ T-cell subsets showed no significant variation em relation to sex, age thymectomy and corticotherapy by monoclonal antibodies.

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Trabalho financiado pelo LIM, HC, FMUSP

Divisão de Neurologia, Hospital das Clínicas, FMUSP - Caixa Postal 3461 - 01051 São Paulo SP - Brasil.

1. Abdou NI, Lisak RP, Zweiman B, Abrahamsohn I, Perm A - The thymus in myasthenia gravis: evidence for altered cell populations. N Engl J Med 291: 1271, 1971.
2. Adner MM, Sherman JD, Isé C, Schwab RS, Dameshek W - An immunologic survey of forty-eight patients with myasthenia gravis. N Engl J Med 271: 1327, 1964.
3. Berrih S, Gaud G, Bach MA, Le Brigand H, Binct JP, Bach JF - Evaluation of T cell subsets in myasthenia gravis using anti-T cell monoclonal antibodies. Clin Exper Immunol 45: 1, 1981.
4. Biesecker G, Koffler D - Immunology of myasthenia gravis. Hum Path 14: 419, 1983.
5. Christadoss P, Dauphine N, Lindstrom J, Dang H, Fernandes G, Talai N - Deficient T cell mitogen response in murine experimental auto immune myasthenia gravis: a defect in the adherent cell population. Cell Immunol 79: 358, 1983.
6. Dosch HM, Shore A - Hypothesis: the role of interleukins in lymphopoiesis are important in autoimmune diseases? J Rheumatol 9: 353, 1982.
7. Dropcho EJ, Richman DP, Antel JP, Arnason BGW - Defective mitogenic response in myasthenia gravis and multiple sclerosis. Ann Neurol 11: 456, 1982.
8. Duarte AJS, Carpenter CB, Strom TB - Expression of T cell differentiation antigens and Ia on rat cytotoxic T lymphocytes. J Immunol 128: 580, 1982.
9. Haynes BF, Harden EA, Orlanow CW, Eisenbarth GS, Wechsler AS, Hensley LL, Roses AD - Effect of thymectomy on peripheral lymphocyte subsets in myasthenia gravis: selective effect on T-cell in patients with thymic atrophy. J Immunol 131: 773, 1983.
10. Jerne JK - Towards a network theory of the immune system. Ann Immunol 125: 373, 1974.
11. Kamo ID, Furukawa S, Tada A, Mano Y, Iwasaki Y, Furuse T - Monoclonal antibody to acetylcholine receptor: cell line established from thymus of patients with myasthenia gravis. Science 215: 995, 1982.
12. Kuroda Y, Oda K, Neshige R, Shibasaki H - Exacerbation of myasthenia gravis after removal of a thymoma having a membrane phenotype of suppressor T cells. Ann Neurol 15: 400. 1984.
13. Landi G, Galli M, Ciboddo G, Colombo R, Scarlato G - Lymphocyte subpopulations and surface membrane immunoglobulins in myasthenia gravis. J Neurol Neurosurg Psychiat 45: 158, 1982.
14. Lisak RP, Zweiman B, Skolnik P, Levinson A, Moskovitz AR, Guerrero F - Thymic lymphocyte subpopulations in myasthenia gravis . Neurology 33: 868, 1983.
15. Mendes N, Kopersztych S, Saraiva S, Assis JL - Imunidade celular na miastenia grave. Rev Hosp Clin Fac Med São Paulo 33: 272, 1978.
16. Miller AE, Hudson J, Tindall RSA - Immune regulation in myasthenia gravis: evidence for in increased suppressor T-cell population. Ann Neurol 12: 348, 1982.
17. Newsom-Davis J, Willcox N, Scadding G, Calder L, Vicent A - Anti-acetylcholine receptors antibody synthesis by cultured lymphocytes in myasthenia gravis: thymic and peripheral blood cell interations. Ann NY Acad Sci 377: 393, 1981.
18. Scadding GK - The role of the thymus in myasthenia gravis. In Rose FC (ed): Clinical Neuroimmunology, Blackwell, Oxford, 1979, pg 128.
19. Shore A, Limatibul S, Dosch HM, Celfand WW - Identification of two serum components regulating the expression of T-lymphocytes function in childhood myasthenia gravis. N Engl J Med 201: 625, 1979.
20. Skolnik PR, Lisak RP, Zweiman B - Monoclonal antibodty analysis of blood T-cell subsets in myasthenia gravis. Ann Neurol 11: 170, 1982.

Estudo dos linfócitos circulantes por anticorpos monoclonais na miastenia grave

Evaluation of circulant lymphocytes in myasthenia gravis by monoclonal antibodies

Datas de Publicação

Publicação nesta coleção
17 Jun 2011
Data do Fascículo
Set 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Abdou NI, Lisak RP, Zweiman B, Abrahamsohn I, Perm A - The thymus in myasthenia gravis: evidence for altered cell populations. N Engl J Med 291: 1271, 1971.

[2] 2. Adner MM, Sherman JD, Isé C, Schwab RS, Dameshek W - An immunologic survey of forty-eight patients with myasthenia gravis. N Engl J Med 271: 1327, 1964.

[3] 3. Berrih S, Gaud G, Bach MA, Le Brigand H, Binct JP, Bach JF - Evaluation of T cell subsets in myasthenia gravis using anti-T cell monoclonal antibodies. Clin Exper Immunol 45: 1, 1981.

[4] 4. Biesecker G, Koffler D - Immunology of myasthenia gravis. Hum Path 14: 419, 1983.

[5] 5. Christadoss P, Dauphine N, Lindstrom J, Dang H, Fernandes G, Talai N - Deficient T cell mitogen response in murine experimental auto immune myasthenia gravis: a defect in the adherent cell population. Cell Immunol 79: 358, 1983.

[6] 6. Dosch HM, Shore A - Hypothesis: the role of interleukins in lymphopoiesis are important in autoimmune diseases? J Rheumatol 9: 353, 1982.

[7] 7. Dropcho EJ, Richman DP, Antel JP, Arnason BGW - Defective mitogenic response in myasthenia gravis and multiple sclerosis. Ann Neurol 11: 456, 1982.

[8] 8. Duarte AJS, Carpenter CB, Strom TB - Expression of T cell differentiation antigens and Ia on rat cytotoxic T lymphocytes. J Immunol 128: 580, 1982.

[9] 9. Haynes BF, Harden EA, Orlanow CW, Eisenbarth GS, Wechsler AS, Hensley LL, Roses AD - Effect of thymectomy on peripheral lymphocyte subsets in myasthenia gravis: selective effect on T-cell in patients with thymic atrophy. J Immunol 131: 773, 1983.

[10] 10. Jerne JK - Towards a network theory of the immune system. Ann Immunol 125: 373, 1974.

[11] 11. Kamo ID, Furukawa S, Tada A, Mano Y, Iwasaki Y, Furuse T - Monoclonal antibody to acetylcholine receptor: cell line established from thymus of patients with myasthenia gravis. Science 215: 995, 1982.

[12] 12. Kuroda Y, Oda K, Neshige R, Shibasaki H - Exacerbation of myasthenia gravis after removal of a thymoma having a membrane phenotype of suppressor T cells. Ann Neurol 15: 400. 1984.

[13] 13. Landi G, Galli M, Ciboddo G, Colombo R, Scarlato G - Lymphocyte subpopulations and surface membrane immunoglobulins in myasthenia gravis. J Neurol Neurosurg Psychiat 45: 158, 1982.

[14] 14. Lisak RP, Zweiman B, Skolnik P, Levinson A, Moskovitz AR, Guerrero F - Thymic lymphocyte subpopulations in myasthenia gravis . Neurology 33: 868, 1983.

[15] 15. Mendes N, Kopersztych S, Saraiva S, Assis JL - Imunidade celular na miastenia grave. Rev Hosp Clin Fac Med São Paulo 33: 272, 1978.

[16] 16. Miller AE, Hudson J, Tindall RSA - Immune regulation in myasthenia gravis: evidence for in increased suppressor T-cell population. Ann Neurol 12: 348, 1982.

[17] 17. Newsom-Davis J, Willcox N, Scadding G, Calder L, Vicent A - Anti-acetylcholine receptors antibody synthesis by cultured lymphocytes in myasthenia gravis: thymic and peripheral blood cell interations. Ann NY Acad Sci 377: 393, 1981.

[18] 18. Scadding GK - The role of the thymus in myasthenia gravis. In Rose FC (ed): Clinical Neuroimmunology, Blackwell, Oxford, 1979, pg 128.

[19] 19. Shore A, Limatibul S, Dosch HM, Celfand WW - Identification of two serum components regulating the expression of T-lymphocytes function in childhood myasthenia gravis. N Engl J Med 201: 625, 1979.

[20] 20. Skolnik PR, Lisak RP, Zweiman B - Monoclonal antibodty analysis of blood T-cell subsets in myasthenia gravis. Ann Neurol 11: 170, 1982.