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Comparison between calcium hydroxide mixtures and mineral trioxide aggregate in primary teeth pulpotomy: a randomized controlled trial

Abstract

Objectives:

To evaluate the effect of calcium hydroxide (CH) associated with two different vehicles as a capping material for pulp tissue in primary molars, compared with mineral trioxide aggregate (MTA).

Methodology:

Forty-five primary mandibular molars with dental caries were treated by conventional pulpotomy using one of the following materials: MTA only (MTA group), CH with saline (CH+saline group) and CH with polyethylene glycol (CH+PEG group) (15 teeth/group). Clinical and periapical radiographic examinations of the pulpotomized teeth were performed 3, 6, and 12 months after treatment. Data were tested by chi-squared analysis and a multiple comparison post-test.

Results:

The MTA group showed both clinical and radiographic treatment success in 14/14 teeth (100%), at all follow-up appointments. By clinical evaluation, no teeth in the CH+saline and CH+PEG groups had signs of mobility, fistula, swelling or inflammation of the surrounding gingival tissue. However, in the CH+saline group, radiographic analysis detected internal resorption in up to 9/15 teeth (67%), and inter-radicular bone resorption and furcation radiolucency in up to 5/15 teeth (36%), from 3 to 12 months of follow-up. In the CH+PEG group, 2/11 teeth (18%) had internal resorption and 1/11 teeth (9%) presented bone resorption and furcation radiolucency at all follow-up appointments.

Conclusion:

CH with PEG performed better than CH with saline as capping material for pulpotomy of primary teeth. However, both combinations yielded clinical and radiographic results inferior to those of MTA alone.

Keywords:
Calcium hydroxide; Deciduous tooth; Mineral trioxide aggregate; Pulpotomy; Randomized trial

Introduction

Pulpotomy is a vital pulp therapy for deciduous teeth in which the coronal pulp tissue is surgically removed and a suitable material is placed on the remaining radicular pulp to protect it from further injury.11- Havale R, Anegundi RT, Indushekar K, Sudha P. Clinical and radiographic evaluation of pulpotomies in primary molars with formocresol, glutaraldehyde and ferric sulfate. Oral Health Dent Manag. 2013;12(1):24-31.,22- Junqueira MA, Cunha NN, Caixeta FF, Marques NC, Oliveira TM, Moretti AB, et al. Clinical, radiographic and histological evaluation of primary teeth pulpotomy using MTA and ferric sulfate. Braz Dent J. 2018;29(2):159-65.,33- Yildirim C, Basak F, Akgun OM, Polat GG, Altun C. Clinical and radiographic evaluation of the effectiveness of formocresol, mineral trioxide aggregate, Portland cement, and enamel matrix derivative in primary teeth pulpotomies: a two year follow-up. J Clin Pediatr Dent. 2016;40(1):14-20.,44- Araújo LB, Cosme-Silva L, Fernandes AP, Oliveira TM, Cavalcanti BD, Gomes Filho JE, et al. Effects of mineral trioxide aggregate, BiodentineTM and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth. J Appl Oral Sci. 2018;26:e20160629,55- Cosme-Silva L, Gomes-Filho JE, Benetti F, Dal-Fabbro R, Sakai VT, Cintra LTA, et al. Biocompatibility and immunohistochemical evaluation of a new calcium silicate-based cement, Bio-C Pulpo. Int Endod J. Forthcoming 2018. doi: 10.1111/iej.13052.
https://doi.org/10.1111/iej.13052...
Pulpotomy aims to retain a functional tooth with a vital radicular pulp in the oral cavity until its exfoliation.11- Havale R, Anegundi RT, Indushekar K, Sudha P. Clinical and radiographic evaluation of pulpotomies in primary molars with formocresol, glutaraldehyde and ferric sulfate. Oral Health Dent Manag. 2013;12(1):24-31.,22- Junqueira MA, Cunha NN, Caixeta FF, Marques NC, Oliveira TM, Moretti AB, et al. Clinical, radiographic and histological evaluation of primary teeth pulpotomy using MTA and ferric sulfate. Braz Dent J. 2018;29(2):159-65.,66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,77- Musale PK, Soni AS. Clinical pulpotomy trial of Copaifera langsdorffii oil resin versus formocresol and white mineral trioxide aggregate in primary teeth. Pediatr Dent. 2016;38(2):5-12.,88- Rajasekharan S, Martens LC, Vandenbulcke J, Jacquet W, Bottenberg P, Cauwels RG. Efficacy of three different pulpotomy agents in primary molars: a randomized control trial. Int Endod J. 2017;50(3):215-28.

Although formocresol was considered the gold standard for pulpotomies in primary teeth, its toxicity and carcinogenic potential have emerged as serious limitations of this capping material.99- Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod. 2014;40(11):1746-51. Numerous studies have investigated the mechanism of action, clinical indication, advantages and disadvantages of different capping materials, but no consensus has yet been reached on the ideal pulpotomy material for the treatment of primary teeth.11- Havale R, Anegundi RT, Indushekar K, Sudha P. Clinical and radiographic evaluation of pulpotomies in primary molars with formocresol, glutaraldehyde and ferric sulfate. Oral Health Dent Manag. 2013;12(1):24-31.,44- Araújo LB, Cosme-Silva L, Fernandes AP, Oliveira TM, Cavalcanti BD, Gomes Filho JE, et al. Effects of mineral trioxide aggregate, BiodentineTM and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth. J Appl Oral Sci. 2018;26:e20160629,66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,88- Rajasekharan S, Martens LC, Vandenbulcke J, Jacquet W, Bottenberg P, Cauwels RG. Efficacy of three different pulpotomy agents in primary molars: a randomized control trial. Int Endod J. 2017;50(3):215-28.,1010- Fernandes AP, Lourenço Neto N, Teixeira Marques NC, Silveira-Moretti AB, Sakai VT, Cruvinel-Silva T, et al. Clinical and radiographic outcomes of the use of Low-Level Laser Therapy in vital pulp of primary teeth. Int J Paediatr Dent. 2015;25(2):144-50.,1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9.

Calcium hidroxide (CH) has been indicated as the most appropriate material in many clinical situations aiming to promote healing. However, the results obtained from pulpotomy using CH-based materials were inconclusive, as long-term clinical trials revealed an increase in failure rates along the follow-up appointments.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,1212- Ozório JE, Carvalho LF, Oliveira DA, Sousa-Neto MD, Perez DE. Standardized propolis extract and calcium hydroxide as pulpotomy agents in primary pig teeth. J Dent Child (Chic). 2012;79(2):53-8. The success rate of CH as a pulpotomy material in primary teeth is poor in comparison to that observed in permanent teeth. The caustic action of the high-pH formulations of CH reduces the size of the underlying dental pulp by up to 0.7 mm.1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50. In addition to the widespread clinical use of CH, studies have tested various CH formulations and mixtures of CH powder with different substances in an attempt to improve CH performance.1212- Ozório JE, Carvalho LF, Oliveira DA, Sousa-Neto MD, Perez DE. Standardized propolis extract and calcium hydroxide as pulpotomy agents in primary pig teeth. J Dent Child (Chic). 2012;79(2):53-8.,1414- Fava LR, Saunders WP. Calcium hydroxide pastes: classification and clinical indications. Int Endod J. 1999;32(4):257-82.

Typically, CH dressing is prepared with either an aqueous (distilled water, saline, anesthetic solution) or viscous (polyethylene glycol, propylene glycol, methylcellulose, glycerin) vehicle.1414- Fava LR, Saunders WP. Calcium hydroxide pastes: classification and clinical indications. Int Endod J. 1999;32(4):257-82.,1515- Oliveira TM, Sakai VT, Silva TC, Santos CF, Machado MA, Abdo RC. Repair of furcal perforation treated with MTA in a primary molar tooth: 20-month follow-up. J Dent Child. 2008;75(2):188-91. When in direct contact with tissues, CH paste prepared with an aqueous vehicle rapidly dissociates into calcium (Ca+2) and hydroxyl (OH) ions, promoting high solubility and becoming easily resorbed by macrophages. In contrast, when CH is mixed with a viscous vehicle, its dissociation is slower, probably because of the high molecular weight of the vehicle, thus minimizing the dispersion of CH into the underlying tissue and maintaining the paste in the desired place for longer.1414- Fava LR, Saunders WP. Calcium hydroxide pastes: classification and clinical indications. Int Endod J. 1999;32(4):257-82.

MTA is considered the gold standard material for pulpotomy of primary teeth.33- Yildirim C, Basak F, Akgun OM, Polat GG, Altun C. Clinical and radiographic evaluation of the effectiveness of formocresol, mineral trioxide aggregate, Portland cement, and enamel matrix derivative in primary teeth pulpotomies: a two year follow-up. J Clin Pediatr Dent. 2016;40(1):14-20.,55- Cosme-Silva L, Gomes-Filho JE, Benetti F, Dal-Fabbro R, Sakai VT, Cintra LTA, et al. Biocompatibility and immunohistochemical evaluation of a new calcium silicate-based cement, Bio-C Pulpo. Int Endod J. Forthcoming 2018. doi: 10.1111/iej.13052.
https://doi.org/10.1111/iej.13052...
,77- Musale PK, Soni AS. Clinical pulpotomy trial of Copaifera langsdorffii oil resin versus formocresol and white mineral trioxide aggregate in primary teeth. Pediatr Dent. 2016;38(2):5-12.,99- Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod. 2014;40(11):1746-51. Its indication is based on its adequate physiochemical and biological properties such as good sealing ability, hydroxyapatite formation and favorable biocompatibility.88- Rajasekharan S, Martens LC, Vandenbulcke J, Jacquet W, Bottenberg P, Cauwels RG. Efficacy of three different pulpotomy agents in primary molars: a randomized control trial. Int Endod J. 2017;50(3):215-28.,1616- Accorinte ML, Loguercio AD, Reis A, Carneiro E, Grande RH, Murata SS, et al. Response of human dental pulp capped with MTA and calcium hydroxide powder. Oper Dent. 2008;33(5):488-95.,1717- Waterhouse PJ, Nunn JH, Whitworth JM, Soames JV. Primary molar pulp therapy - histological evaluation of failure. Int J Paediatr Dent. 2000;10(4):313-21. MTA induces hard tissue formation when used for pulp capping, pulpotomy, perforated furcations, root canal filling and root-end fillings in animals;1212- Ozório JE, Carvalho LF, Oliveira DA, Sousa-Neto MD, Perez DE. Standardized propolis extract and calcium hydroxide as pulpotomy agents in primary pig teeth. J Dent Child (Chic). 2012;79(2):53-8.,1515- Oliveira TM, Sakai VT, Silva TC, Santos CF, Machado MA, Abdo RC. Repair of furcal perforation treated with MTA in a primary molar tooth: 20-month follow-up. J Dent Child. 2008;75(2):188-91.,1717- Waterhouse PJ, Nunn JH, Whitworth JM, Soames JV. Primary molar pulp therapy - histological evaluation of failure. Int J Paediatr Dent. 2000;10(4):313-21. however, it is costly and causes dental pigmentation.1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9.,1818- Cosme-Silva L, Carnevalli B, Sakai VT, Viola NV, Franco de Carvalho L, Franco de Carvalho EM. Radicular perforation repair with mineral trioxide aggregate: a case report with 10-year follow-up. Open Dent J. 2016;30:733-8.,1919- Torabinejad M, Chivian N. Clinical applications of mineral trioxide aggregate. J Endod. 1999;25(3):197-205.

Considering the scarcity of randomized controlled trials testing CH associated with different vehicles as a dressing agent for primary teeth, this study aimed to evaluate the effect of CH prepared with either an aqueous (saline) or a viscous (polyethylene glycol) vehicle as a capping material for the pulpotomy of primary molars. Of note, the effects of CH mixtures were compared with those of MTA, which represents the current standard of treatment for primary teeth pulpotomy.

Methodology

Participants and ethics

This prospective and randomized clinical trial was registered in the ISRCTN (registration number: ISRCTN51317677) and approved by the Ethics Committee of the School of Dentistry (Federal University of Alfenas; protocol #15304613.1.0000.5142). The trial was conducted from July 2013 to July 2015 at the School of Dentistry from the Federal University of Alfenas, involving 39 children on whom 45 pulpotomies were performed. The parents or guardians of the children involved received pertinent information regarding the study and provided written informed consent for their children's participation in the trial. No monetary compensation for their participation was provided. All participants were blinded to the treatment group to which they were allocated.

The inclusion criteria used in this study were: mandibular primary first or second molar of children aged between 5 and 8 years and belonging to the American Society of Anesthesiologists (ASA) physical status category I; no history of pain; adequate clinical macroscopic appearance of the dental pulp (normal consistency, bright red color and cut resistance); no clinical or radiographic evidence of pulp degeneration, such as excessive bleeding, internal root resorption, inter-radicular and/or furcal bone destruction; and no more than one third of physiological root resorption detected in periapical radiographies. The following exclusion criteria were used: excessive pulp bleeding until 5 minutes after removal; dark red colored pulp; little cut resistance; impossibility of restoration, presence of systemic illness and history of allergic reaction to the tested materials, latex or local anesthetics; and teeth with more than two thirds of radicular resorption. Based on the study of Moretti, et al.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55. (2008), a sample size of 15 teeth per group was calculated to provide a power of 80% to detect statistical significance at the level of 5%.

Randomization and blinding

Teeth included in this study were randomly allocated to one of three treatment groups using a computer-generated random number sequence. Each tooth was mapped to a single unique code until the end of the study, and randomization was concealed with the use of a sealed envelope. Based on the randomization, each tooth was allocated to one of the three experimental groups: MTA group, CH+saline group and CH+PEG group. Blinding of the outcome assessment and data analysis was achieved by using codes to designate each technique.

Pulpotomy

Two investigators selected the teeth based on the inclusion criteria, but only one performed the pulpotomies. The investigators had been previously involved in several similar studies and used a standard pulpotomy technique. In all groups, caries removal was performed with a round bur in a slow-speed handpiece after local anesthesia with 2% mepivacaine with 1:100.000 epinephrine and rubber dam isolation. The pulp access cavities were performed with a round carbide bur. Full coronal pulp tissue (of appropriate consistency, bright- and red-colored and with cut resistance) was manually removed with an excavator, and the wound surface was irrigated with saline solution to remove debris and achieve pulp homeostasis. In the MTA group, a paste obtained by mixing gray MTA powder (Ângelus, Londrina, PR, Brazil) with distilled water at a 1:1 powder/liquid ratio, according to the manufacturer's specifications, was placed into the channel orifice in contact with the remaining dental pulp tissue. In the CH+saline group, the remaining pulp tissue was dressed with a paste obtained by mixing calcium hydroxide P.A. (Biodinâmica Química e Farmacêutica Ltda., Ibiporã, PR, Brazil) with sterile normal saline solution (0.9% sodium chloride) at a 1:1 powder/liquid ratio. In the CH+PEG group, the remaining pulp tissue was dressed with a paste obtained by mixing calcium hydroxide P.A. with PEG (MAPRIC - Produtos Farmacêuticos e Cosméticos Ltda., São Paulo, SP, Brazil) at a 1:1 powder/liquid ratio. In all groups, a 1-mm thick layer of material was used for capping, followed by another 1-mm thick of a layer of cement-cured calcium hydroxide (Biodinâmica Química e Farmacêutica Ltda., Ibiporã, PR, Brazil), employed as an intermediate base for the restoration with resin-modified glass-ionomer cement (RMGIC) (Vitremer; 3M ESPE, São Paulo, SP, Brazil).

Clinical analysis and radiography

Periodic follow-up examinations were carried out 3, 6, and 12 months after treatment. At each follow-up appointment, two blinded and calibrated investigators performed clinical and periapical radiographic examinations of the pulpotomized teeth, as previously described.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9.,1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50. Inter- and intra-rater agreements were estimated as Kappa coefficients of 0.83 and 0.97, respectively. Patients were instructed on how to brush their teeth, and were encouraged to brush twice a day.

Standardized periapical or interproximal radiographs were taken with the use of conventional films (Sensitivity Insight films of the Kodak14 Mark, size #1) and holders, using an exposure time of 0.5 seconds.

Clinical success was defined as the lack of spontaneous pain, mobility, swelling, or fistula in the treated tooth. Lack of internal or external root resorption and furcation radiolucency were indicative of radiographic success. Data were tested by chi-squared analysis and a multiple comparison post-test, with statistical significance set at p<0.05.

Results

Forty-five primary molars in children with mean age of 6 years and 5 months filled the inclusion criteria and were allocated to one of the three treatment groups (15 teeth per group) (Figure 1).

Figure 1
Flow of patients and pulpotomized teeth up to 12 months

In all treatment groups, no tooth showed mobility, fistula, swelling or inflammation on the surrounding gingival tissue by clinical examination, at any follow-up appointments. In the MTA group, treatment was also successful by radiographic analysis in 14/14 teeth (100%). Thus, both clinical and radiographic analyses showed 100% treatment success using MTA, at all follow-up appointments (Figure 1; Table 1).

Table 1
Radiographic success (S) and failure (F) rates for the use of MTA, CH+saline and CH+PEG as capping agents in pulpotomies, at 3-, 6- and 12-month follow-up

Despite the absence of clinical symptomatology indicative of treatment failure in the CH+saline and CH+PEG groups, we observed some radiographic evidence of treatment failure in these groups (Table 1). In the CH+saline group, internal resorption indicative of treatment failure was radiographically detected in up to 9/15 teeth (67%), at 12-month follow-up (Table 2). Inter-radicular bone destruction and furcation radiolucency, which also provide evidence of treatment failure, were observed in up to 5/15 teeth (36%), at 12-month follow-up (Table 3). Consequently, the final success rate of treatment in the CH+saline group was only 33% (5/15 teeth), after 12 months of follow-up (Table 1).

Table 2
Internal resorption radiographically observed for MTA, CH+saline and CH+PEG pulpotomies at 3-, 6-, and 12-month follow-up
Table 3
Inter-radicular bone destruction radiographically observed for MTA, CH+saline and CH+PEG pulpotomies at 3-, 6-, and 12-month follow-up

In the CH+PEG group, representing teeth capped with CH mixed with PEG, 2/12 teeth (17%), 2/11 (18%) and 2/11(18%) had internal resorption at the 3, 6 and 12 months of follow-up, respectively (Table 2), while one tooth also presented bone destruction and furcation radiolucency at all follow-up appointments (Table 3), being referred for endodontic treatment. The final radiographic success rate of the use of CH+PEG at 12-month follow-up was 73% (8/11 teeth).

The incidence of internal resorption in the CH+saline group was significantly higher than that in the MTA group, at all follow-up appointments (p=0.058; p= 0,019; p=0.0005). No statistically significant difference in the incidence of internal resorption was observed between the MTA group and the CH+PEG group throughout follow-up (Table 2).

Regarding inter-radicular bone destruction, no statistically significant difference between groups was found at 3 months and 6 months (p=0.190; p=0.090). At 12 months of follow-up, the CH+saline group had an increased incidence of radiographic failure compared with the MTA group (p=0.026). Representative examples of radiographs taken during follow-up are shown in Figure 2.

Figure 2
Sample radiographs representative of radiographic changes during follow-up. Arrows indicate the region of interest. (a) Preoperative radiograph; (b) Internal root resorption without perforation; (c) Internal root resorption with perforation; (d) Inter-radicular bone destructions; (e) Radiographic success; (f) Tooth too close to exfoliation

Discussion

In this study, we assessed the suitability of three different dressing agents (MTA, CH+saline and CH+PEG) for pulpotomy of primary teeth. This randomized clinical trial was carried out following a well established methodology employed previously regarding the inclusion and exclusion criteria, sample size, follow-up periods and clinical and radiographic criteria to determine the success or failure of pulpotomies.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9.,1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50.

Pulpotomies with MTA had excellent outcomes over the entire follow-up period, while those employing CH-based capping materials had poorer outcomes, despite the fact that both materials have similar active chemical composition, which is directly influenced by its high pH. For both materials, the contact with vital tissues results in the dissociation of Ca2+ and OH ions. The Ca2+ ions react with carbon dioxide present in the tissue, resulting into calcite crystals and, thus, hard tissue deposition, while the OH ions have antimicrobial effect and cause pulp necrosis, which triggers tissue repair.11- Havale R, Anegundi RT, Indushekar K, Sudha P. Clinical and radiographic evaluation of pulpotomies in primary molars with formocresol, glutaraldehyde and ferric sulfate. Oral Health Dent Manag. 2013;12(1):24-31.,33- Yildirim C, Basak F, Akgun OM, Polat GG, Altun C. Clinical and radiographic evaluation of the effectiveness of formocresol, mineral trioxide aggregate, Portland cement, and enamel matrix derivative in primary teeth pulpotomies: a two year follow-up. J Clin Pediatr Dent. 2016;40(1):14-20.,77- Musale PK, Soni AS. Clinical pulpotomy trial of Copaifera langsdorffii oil resin versus formocresol and white mineral trioxide aggregate in primary teeth. Pediatr Dent. 2016;38(2):5-12. The contrasting results with MTA and CH may be due to marginal differences in sealing ability as a result of distinct solubility or mechanical properties.1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9.,1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50.,1717- Waterhouse PJ, Nunn JH, Whitworth JM, Soames JV. Primary molar pulp therapy - histological evaluation of failure. Int J Paediatr Dent. 2000;10(4):313-21. The ability to maintain contact with the pulp tissue during the entire treatment period seems to be the main factor responsible for the excellent results with the use of MTA.1919- Torabinejad M, Chivian N. Clinical applications of mineral trioxide aggregate. J Endod. 1999;25(3):197-205.

Although CH+saline is commonly employed as a capping material for the pulp therapy of permanent teeth, this capping mixture was tested because results are contradictory regarding its clinical and radiographic success rates for the treatment of primary teeth.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,1212- Ozório JE, Carvalho LF, Oliveira DA, Sousa-Neto MD, Perez DE. Standardized propolis extract and calcium hydroxide as pulpotomy agents in primary pig teeth. J Dent Child (Chic). 2012;79(2):53-8.,1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50.,2020- Percinoto C, Castro AM, Pinto LM. Clinical and radiographic evaluation of pulpotomies employing calcium hydroxide and trioxide mineral aggregate. Gen Dent. 2006:54(4):258-61.,2121- Salako N, Joseph B, Ritwik P, Salonen J, John P, Junaid TA. Comparison of bioactive glass, mineral trioxide aggregate, ferric sulfate and formocresol as pulpotomy agents in rat molars. Dent Traumatol. 2003;19(6):314-20. Moreover, CH-based materials tend to dissolve over time and leave a void underneath the restoration.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,99- Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod. 2014;40(11):1746-51. To minimize this phenomenon, CH+PEG was also tested because the association of CH with a viscous vehicle could enhance the chemical and biological potential of the paste due to the slow release of Ca2+ and OH ions.1414- Fava LR, Saunders WP. Calcium hydroxide pastes: classification and clinical indications. Int Endod J. 1999;32(4):257-82.

Nelson-Filho, et al.2222- Nelson-Filho P, Venturini DP, Bezerra da Silva RA, Fiori-Junior M, Mori LB. Mineral trioxide aggregate and calcium hidroxide utilized in pulpotomies in human primary teeth- clinical and radiographic study. J Health Sci Inst. 2005;23:211-6. (2005) reported 100% of clinical success, as well as 93% and 86.6% of radiographic success for MTA and CH with saline, respectively, after 6 months. Percinoto, Castro and Pinto2020- Percinoto C, Castro AM, Pinto LM. Clinical and radiographic evaluation of pulpotomies employing calcium hydroxide and trioxide mineral aggregate. Gen Dent. 2006:54(4):258-61. (2006) reported that three out of forty-five teeth capped with CH failed after three months, two cases failed after six months and one failed after one year, while just two failures were found using MTA, after one year. Yildiz & Tosun2323- Yildiz E, Tosun G. Evaluation of formocresol, calcium hydroxide, ferric sulfate, and MTA primary molar pulpotomies. Eur J Dent. 2014;8(2):234-40. (2014) concluded that the success rates of MTA and CH were 96.4% and 85%, respectively, after 30 months of follow-up. In contrast, after 36 months, Huth, et al.1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50. (2012) concluded that the radiographic success rate for pulpotomy with CH was 46% only. In our study, the success rate of CH+saline group decreased from 67% at 3 months follow-up to 33% at 12 months follow-up, in line with the results by Huth, et al.1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50. (2012), Mente, et al.99- Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod. 2014;40(11):1746-51. (2014) and Moretti, et al.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55. (2008). The progressive failure of pulpotomies using CH+saline may be due to the high solubility (through the radicular pulp tissue) of CH particles in the medium. When CH+PEG group was used, fewer failures (27%) were detected, which may be attributed to the incorporation of a viscous vehicle to the CH powder. The higher molecular weight of the viscous vehicle compared to the aqueous vehicles is likely to have reduced the dispersion of the CH powder through the pulp tissue, keeping the paste into direct contact with the tissue for longer.1414- Fava LR, Saunders WP. Calcium hydroxide pastes: classification and clinical indications. Int Endod J. 1999;32(4):257-82.

Internal resorption is the most frequent reason for failure following pulpotomy with CH in primary teeth, which indicates that, despite pulp vitality, a silent chronic inflammation develops after treatment with CH and remains undiagnosed, thus triggering odontoclast activity.1010- Fernandes AP, Lourenço Neto N, Teixeira Marques NC, Silveira-Moretti AB, Sakai VT, Cruvinel-Silva T, et al. Clinical and radiographic outcomes of the use of Low-Level Laser Therapy in vital pulp of primary teeth. Int J Paediatr Dent. 2015;25(2):144-50.,1515- Oliveira TM, Sakai VT, Silva TC, Santos CF, Machado MA, Abdo RC. Repair of furcal perforation treated with MTA in a primary molar tooth: 20-month follow-up. J Dent Child. 2008;75(2):188-91. Inappropriate operative techniques may also result in internal resorption if a thick blood clot remains or pulp misdiagnosis occurs.2020- Percinoto C, Castro AM, Pinto LM. Clinical and radiographic evaluation of pulpotomies employing calcium hydroxide and trioxide mineral aggregate. Gen Dent. 2006:54(4):258-61.,2323- Yildiz E, Tosun G. Evaluation of formocresol, calcium hydroxide, ferric sulfate, and MTA primary molar pulpotomies. Eur J Dent. 2014;8(2):234-40.,2424- Oliveira TM, Moretti AB, Sakai VT, Lourenço Neto N, Santos CF, Machado MA, et al. Clinical, radiographic and histologic analysis of the effects of pulp capping materials used in pulpotomies of human primary teeth. Eur Arch Paediatr Dent. 2013;14(2):65-71. Thus, bleeding control after coronal pulp amputation may significantly influence the outcome of pulpotomies with CH.2323- Yildiz E, Tosun G. Evaluation of formocresol, calcium hydroxide, ferric sulfate, and MTA primary molar pulpotomies. Eur J Dent. 2014;8(2):234-40.,2424- Oliveira TM, Moretti AB, Sakai VT, Lourenço Neto N, Santos CF, Machado MA, et al. Clinical, radiographic and histologic analysis of the effects of pulp capping materials used in pulpotomies of human primary teeth. Eur Arch Paediatr Dent. 2013;14(2):65-71. Some authors suggested that avoiding blood clot formation between the pulp tissue and the CH cap prevents the occurrence of internal resorption,1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9. however, this is technically difficult since the incision into vital tissue produces both haemorrhage and exudation.1717- Waterhouse PJ, Nunn JH, Whitworth JM, Soames JV. Primary molar pulp therapy - histological evaluation of failure. Int J Paediatr Dent. 2000;10(4):313-21.,2525- Schröder U. Effect of an extra-pulpal blood clot on healing following experimental pulpotomy and capping with calcium hydroxide. Odontol Revy. 1973;24(3):257-68. In the present study, we cannot attribute the occurrence of internal resorption to differences in the operative technique, since only one investigator performed all pulpotomies. In addition, the same method of bleeding control was employed in the three groups and no internal resorption was detected in the MTA group. It is worth mentioning that MTA has the advantage of hardening in the presence of moisture,2626- Nekoofar MH, Oloomi K, Sheykhrezae MS, Tabor R, Stone DF, Dummer PM. An evaluation of the effect of blood and human serum on the surface microhardness and surface microstructure of mineral trioxide aggregate. Int Endod J. 2010;43(10):849-58. which makes it suitable for use in areas where generating a dry environment is virtually impossible, such as pulp chambers.1212- Ozório JE, Carvalho LF, Oliveira DA, Sousa-Neto MD, Perez DE. Standardized propolis extract and calcium hydroxide as pulpotomy agents in primary pig teeth. J Dent Child (Chic). 2012;79(2):53-8.,66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.

Following the guidelines of a prior study by Moretti, et al.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55. (2015), we categorized internal resorption as radiographic failure, and the affected teeth were monitored during follow-up. We chose not to treat the affected teeth immediately because pathological findings in primary teeth may not require intervention, as primary teeth have limited survival and the pathology may not necessarily affect the permanent successor. However, most cases of internal resorption observed in CH-treated teeth in the present study developed into osseous changes whose clinical signs and symptoms were detected later on during follow-up, in agreement with that reported by Moretti, et al.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55. (2015). Eventually, extraction of the affected teeth was necessary.

Dental pulp healing depends on several factors, including the presumed stimulatory effect of the capping agent, and the ability of both the capping and definitive restorative materials to seal the tooth restoration interface against immediate and long-term microleakage.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,2020- Percinoto C, Castro AM, Pinto LM. Clinical and radiographic evaluation of pulpotomies employing calcium hydroxide and trioxide mineral aggregate. Gen Dent. 2006:54(4):258-61. In pulpotomies with CH, zinc oxide eugenol cement is commonly used before dental restoration.1010- Fernandes AP, Lourenço Neto N, Teixeira Marques NC, Silveira-Moretti AB, Sakai VT, Cruvinel-Silva T, et al. Clinical and radiographic outcomes of the use of Low-Level Laser Therapy in vital pulp of primary teeth. Int J Paediatr Dent. 2015;25(2):144-50. However, the fact that the CH paste dissolves easily could favor the contact of the healthy radicular pulp with the eugenol, which is considered aggressive, and could lead to failures.66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55. To avoid this effect, a resin-based CH cement was placed over the CH pastes as an intermediate base. This material has lower solubility and water sorption than the CH paste, increasing restoration longevity.2727- Francisconi LF, Freitas AP, Scaffa PM, Mondelli RF, Francisconi PA. Water sorption and solubility of different calcium hydroxide cements. J Appl Oral Sci. 2009;17(5):427-31. Additionally, the CH cement is of low strength2828- Tam LE, Pulver E, McComb D, Smith DC. Physical properties of calcium hydroxide and glass-ionomer base and lining materials. Dent Mater. 1989;5(3):145-9. and is highly resistant to etchant,2929- Burke FJ, Watts DC. Weight loss of three resin-based lining materials containing calcium following a phosphoric acid-etching and washing cycle. J Dent. 1989;17(1):38-40. which is necessary for restorations with resin modified glass ionomer cement (RMGIC). Although stainless steel crowns provide optimal coronal seal,66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,1313- Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012;16(4):1243-50.,1717- Waterhouse PJ, Nunn JH, Whitworth JM, Soames JV. Primary molar pulp therapy - histological evaluation of failure. Int J Paediatr Dent. 2000;10(4):313-21. in the present study and in several others66- Moretti AB, Fornetti APC, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpotomies in primary teeth. Int Endod J. 2008;41(7):547-55.,1010- Fernandes AP, Lourenço Neto N, Teixeira Marques NC, Silveira-Moretti AB, Sakai VT, Cruvinel-Silva T, et al. Clinical and radiographic outcomes of the use of Low-Level Laser Therapy in vital pulp of primary teeth. Int J Paediatr Dent. 2015;25(2):144-50.,1111- Sakai VT, Moretti AB, Oliveira TM, Fornetti AP, Santos CF, Machado MA, et al. Pulpotomy of human primary molars with MTA and Portland cement: a randomised controlled trial. Br Dent J. 2009;207(3):128-9.,2424- Oliveira TM, Moretti AB, Sakai VT, Lourenço Neto N, Santos CF, Machado MA, et al. Clinical, radiographic and histologic analysis of the effects of pulp capping materials used in pulpotomies of human primary teeth. Eur Arch Paediatr Dent. 2013;14(2):65-71.,3030- Marques NC, Neto NL, Rodini CO, Fernandes AP, Sakai VT, Machado MA, et al. Low-level laser therapy as an alternative for pulpotomy in human primary teeth. Lasers Med Sci. 2015;30(7):1815-22 involving Class I and II cavities of primary molars in a high caries risk population, final restorations were performed with RMGIC. According to the guidelines of the American Association of Pediatric Dentistry (AAPD), there is strong evidence that RMGIC is efficient for Class I restorations, and expert opinion supports the use of RMGIC for Class II restorations in primary teeth,3131- American Academy of Pediatric Dentistry Reference Manual 2016. Guideline on Restorative Dentistry. Pediatr Dent. 2016;38:250-62. in line with a systematic review recommending the use of RMGIC in small to moderate sized Class II cavities.3232- Chadwick BL, Evans DJ. Restoration of class II cavities in primary molar teeth with conventional and resin modified glass ionomer cements: a systematic review of the literature. Eur Arch Paediatr Dent. 2007;8(1):14-21. In support of the current recommendations, no restoration failures were observed in this study for any of the three groups throughout follow-up; thus, we can exclude restorative material as a discriminating factor between groups, in this study.

The limitations of this study are inherent to randomized clinical trials, where patients must return for follow-up and complete “blinding” may be challenging. In this study, 5 patients (one in the MTA group and four in the CH+PEG group) were lost at follow-up, because they were unwilling to travel from their hometown to report to the clinic. Moreover, it was impossible to blind the operator at the moment the material was inserted, since MTA and CH had different mixing methods. In addition, blinding the rater for the radiographic examination is not fail proof, as differences in radiopacity between materials can be identified by experienced examiners.

Conclusion

The association of CH with PEG provided better results than that of CH with saline as a capping material for pulpotomy of primary teeth. However, both associations demonstrated clinical and radiographic results inferior to those of MTA.

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Publication Dates

  • Publication in this collection
    20 May 2019
  • Date of issue
    2019

History

  • Received
    17 Jan 2018
  • Reviewed
    06 Oct 2018
  • Accepted
    11 Nov 2018
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