versão impressa ISSN 0001-3765
An. Acad. Bras. Ciênc. v.72 n.2 Rio de Janeiro jun. 2000
CHEMICAL AND BIOLOGICAL INVESTIGATIONS ON Nidularium innocentii LEMAIRE*
Luciana Moreira Chedier1,2, Maria Raquel Figueiredo1 and Maria Auxiliadora C. Kaplan2,3
1Laboratório de Química de Produtos Naturais/PN3, Far-Manguinhos/FIOCRUZ.
2Museu Nacional, Universidade Federal do Rio de Janeiro.
3Núcleo de Pesquisas de Produtos Naturais/Universidade Federal do Rio de Janeiro.
Nidularium innocentii Lemaire belongs to the family Bromeliaceae, order Bromeliales, superorder Liliiflorae ( sensu Dahlgren, 1982). This family consists of 50 genera and about 2500 species, many of which have economic value as Ananas comosus (L.) Merril (pineapple) or are used in popular medicine as anti-inflammatory, analgesic, anti-helmintic and diuretic agents. Among the secondary metabolites, flavonoids, terpenoids and steroids are the common representatives. N. innocentii was collected at Poço das Antas National Park, Silva Jardim, RJ. Leaves and rhizomes/roots after drying, were ground and submitted in sequence to extraction with hexane and methanol, yielding the hexane and methanol extracts. Methanol extract of rhizomes/roots suspended in water were submitted to liquid-liquid partitions with hexane, dichloromethane, ethyl acetate and butanol. All the extracts and fractions have been evaluated for analgesic, anti-inflammatory, anti-neoplasic and tripanosomicidal activities. The hexane fraction of rhizomes/roots methanol extract was submitted to silicagel adsorption chromatography. 3-Oxo-friedelin was obtained from a fraction eluted with hexane/ethyl acetate 5% and the steroids campesterol, sitosterol and stigmasterol were eluted with hexane/ethyl acetate 15%. The hexane extract of leaves upon chromatographic techniques yielded a fusidane derivative. The compounds were identified by spectroscopic analysis.
( November 23, 1999 ) .
* Supported by: CNPq/CAPES.