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Anais da Academia Brasileira de Ciências

versão impressa ISSN 0001-3765versão On-line ISSN 1678-2690

An. Acad. Bras. Ciênc. vol.90 no.3 Rio de Janeiro jul./set. 2018

http://dx.doi.org/10.1590/0001-3765201820170914 

Biological Sciences

Wollastonite to hinder growth of Aspergillus niger fungus on cotton textile

HAMID R. TAGHIYARI1 

REZA MAJIDINAJAFABADI2 

REZA VAHIDZADEH2 

1Wood Science and Technology Department, Faculty of Materials Engineering and New Technologies, Shahid Rajaee Teacher Training University, Lavizan, 22970021 Tehran, Iran

2Department of Restoration, Islamic Azad University, Central Tehran Branch/IAU - CTB, Poonak Square, Ashrafi Esfehani Avenue, 9399807079 Tehran, Iran

Abstract

Effects of Aspergillus niger was investigated on the strength of cotton textile specimens impregnated with nano-wollastonite, and then compared with normal specimens. Cotton strips were cut and prepared in warp and wept directions according to the standard specifications ASTM D-5035. Results showed that incubation of A. niger on specimens for three months resulted in a significant decrease in tensile stress as well as weight mass change in both directions. Impregnating specimens with NW ameliorated the negative effects of fungal attack on tensile stress to a considerable extent. Moreover, weight change was significantly decreased. It is concluded that NW positively protect cotton textile against A. nigra; the ultimate NW-content should be studied in complimentary studies.

Key words biological resistance; cotton fabric; fungal degradation; mineral materials; textile; wollastonite

INTRODUCTION

Different fungi spores are suspended in the air and are moved easily by air. This puts precious materials made from cellulosic materials at great risk of contamination and disintegration (Grace et al. 2017, Tanny et al. 2017, Tupciauskas et al. 2017). Many biological materials are vulnerable to ordinary fungi, being deteriorated by them (Seephueak et al. 2018). Cellulose and ligno-cellulose materials are amongst the materials that can be degraded by different fungi (Schmidt 2006, 2007, Abdel-Kareem 2010a, b, Maresi et al. 2013).

The main ingredient of cotton textiles is cellulose, a bio-polymer. This makes cotton textiles vulnerable to a wide range of different fungi, including Aspergillus, Penicillium, Chaetomium, Trichoderms, and Alternaria species (Abdel-Kareem 2010a). Exposure of historical objects, including papers, textiles, and wood, to deteriorating fungi is inevitable. Therefore, different polymers, fungicides, and nano-materials have so far been used to increase biological resistance of the materials to different fungi (Abdel-Kareem 2010a). Abdel-Kareem (2010b) treated textile samples with four polymers mixed with two kinds of fungicide. The samples were exposed to some dominant fungi isolated from ancient Egyptian textiles. The results of this study showed increase in the durability of the tested textiles; moreover, the textiles were reinforced.

Cellulosic materials are vulnerable to different wood-deteriorating fungi and molds (Elaieb et al. 2017, Morias et al. 2018). Metal and mineral nano-materials have also been used to improve biological resistance of wood and wood composites to different fungi (Karimi et al. 2013, Taghiyari et al. 2014a, b, Bari et al. 2015, 2017, Taghiyari et al. 2013, 2015), and to improve many properties in different materials (Pethig 2017, Sandeep et al. 2017). Nano-zincoxide and nano-copper cannot be recommended as a fungicide for historical textiles due to their health problems to human though they have demonstrated high efficacy against Trametes versicolor fungus (Taghiyari et al. 2015). Therefore, attention was drawn to some other materials with lower hazard to human health (Taghiyari et al. 2014a, b). Wollastonite is a calcium-silicate-based mineral that has been reported to have no proven hazards to human health (Huuskonen et al. 1983a, b, Maxim and McConnell 2005). Experiments showed its high efficacy to different wood and paper deteriorating fungi, including Aspergillus niger (Taghiyari et al. 2014a-c).

A recent study illustrated that wollastonite nano-fibers were effective in hindering the growth of Aspergillus niger on historical papers (Taghiyari et al. 2014c). However, authors came across little or no study on the exposure of NW-treated cotton textile to any fungi. In this connection, cotton textile may react similarly or differently as regard to wood, wood composites, and even papers in which cellulose has a different structure. The present study, therefore, focused on possible improvement in biological resistance of cotton textile caused by NW treatment, against Aspergillus niger, as a dominant fungus in Moghadam Museum of University of Tehran.

MATERIALS AND METHODS

SPECIMEN PREPARATION

Iranian cotton fabric was purchased from Tehran Central Bazaar. Separate sets of strips were cut in the longitudinal (warp) and transverse (wept) directions of the cotton fabric according to the standard specifications ASTM D-5035. Dimensions of both warp and wept strips were 80 × 30 mm. Threads were removed from both sides of all warp and wept strips to produce final width of 20 mm, leaving fringes on both width sides (Fig. 1, b). Strips of both warp and wept directions were randomly divided into three main groups of control (C), fungus-exposed (FE), and nanowollastonite-impregnated fungus-exposed (NW-FE) specimens. Ten replications were produced for each of the groups; totally, 60 specimens were cut and prepared.

Figure 1 Photographs of warp (a) and wept (b) control samples.  

The size range of wollastonite nanofibers in the present project was 30 – 110 nm. Specifications of wollastonite compounds and formulations are presented in Table I. Previous studies showed that NW with 20% concentration demonstrated optimum hindering effect on fungal growth (Taghiyari et al. 2014b, c). Therefore, 20%-NW-suspension was prepared for immersion of the cotton strips. The NW-impregnated specimens were hanged to dry for four weeks before being exposed to the experimental fungus along with the control specimens.

TABLE I Compounds of the nano-wollastonite gel used for treatment purpose (Taghiyari et al. 2014a-c). 

Nano-wollastonite compounds Mixing ratio by mass (%)
CaO 39.77
SiO2 46.96
Al2O3 3.95
Fe2O3 2.79
TiO2 0.22
K2O 0.04
MgO 1.39
Na2O 0.16
SO3 0.05
Water 4.67

FUNGAL EXPOSURE

Aspergillus niger (van Tieghem) is a common fungus species of the genus Aspergillus that belongs to Ascomycota. It is the cause of a disease called black mold and is considered a common contaminant of different food stuff, including fruits, vegetables, and cellulosic materials (Alexopoulos et al. 1996). The cotton fabric specimens were dried, at 103±2ºC for 24h in a hot air oven, and weighted before being exposed to the test fungus for three months in Petri dishes. Once the incubation period was passed, they were again weighted to measure weight change. The incubation process was carried out at the National Library and Archives of I. R. of Iran under 25±1ºC and 45±2% relative humidity on Sabouraud’s agar. It is to be noted that A. niger generally needs higher relative humidity and lower temperature for ultimate damage; however, in the present study, the deteriorating effect of the fungus under real conditions was the ultimate target. Once the incubation period passed, mycelia were removed from the specimens; care was taken not to disturb the textile texture. The specimens were again dried at 103±2ºC for 24h and weighed to determine the fungal mass change using Equation No. 1:

MC(% )=M1M2M1x100 (1)

where MC is the mass change (%), M1 is the dry matter before incubation (g), and M2 is the dry matter after incubation (g). It is to be noted that in the present study, it was preferred to use “mass change” instead of “mass loss” as it is customarily used in papers. The reason will be further discussed that an increase was observed in the weight of specimens exposed to the test fungus.

TENSILE TEsT PROCEDURE

Tensile tests were carried out according to ASTM D-5035 standard specification by an Instron 5566 universal test machine (manufactured in the USA). The initial distance between the two jaws was fixed to 50 mm for specimens of all control, FE, and NW-FE treatments. Loading speed was 40 mm/min.

DENSITY FUNCTIONAL THEORY (DFT)

OpenMX3.6 package (Majidi 2012, 2016) was utilized for density functional theory (DFT) to simulate the adsorption of NW on cellulose surface at molecule level (Taghiyari et al. 2016). The generalized gradient approximation (GGA) function with the Perdew-Burke-Ernzerhof (PBE) correction was carried out to describe the exchange-correlation energy functional (Perdew et al. 1996). The van der Waals (vdW) interactions were included by DFT-D2 approach proposed by Grimme (2006).

STATISTICAL ANALYSIS

Statistical analysis was conducted using SAS software program, version 9.2 (2009). Two-way analysis of variance (ANOVA) was performed to discern significant difference at the 95% level of confidence. Grouping was then made between treatments, using the Duncan’s multiple range test. Hierarchical cluster analysis, including dendrogram and using Ward methods with squared Euclidean distance intervals, was carried out by SPSS/18 (2010) (Ada 2013). Fitted-line plots were made by Minitab software, version 16.2.2 (2010).

RESULTS AND DISCUSSION

Weight measurements before and after NW-impregnation demonstrated that NW-uptake values were 30 and 25 g.m-2 in warp and wept specimens, respectively. Visual observation demonstrated that after three months of exposure to Aspergillus nigra, mycelia of the fungus nearly covered all parts of the cotton specimen in the Petri dish (Fig. 2 a). However, NW-impregnated specimens illustrated some resistance to the growth of fungus hyphae (Fig. 2b); the fact that the adjacent Sabouraud’s agar were completely covered by mycellium of the fungus implied that wollastonite practically hindered the growth of fungus on the NW-impregnated specimens.

Figure 2 Visual observation of the control (a) and NW-impregnated (b) specimens after three months of exposure to A. nigra in Petri dish.  

The results of the weight measurements before and after being exposed to A. nigra demonstrated that all cotton textile specimens were increased in weight after the exposure to the fungus. This increase in weight was due to the penetration of mycelium of the fungus into the texture of cotton textile; that is, mycelium was initially fed with Sabouraud’s agar and grew into cotton texture. In the control specimens and after the initial stage when Sabouraud’s agar was used up, mycelium also consumed the cotton textile; however, the amount used Sabouraud’s agar was higher than cotton texture, leading to the increase in weight. In this regard, specimens in wept directions had higher weight changes both in the control and NW-impregnated specimens in comparison to warp specimens (Fig. 3a). The highest and lowest weight changes occurred in the control specimens in wept direction (4.37%) and NW-impregnated specimens in the warp direction (0.9%), respectively. Impregnation of the cotton specimens with wollastonite significantly decreased weight changes in both directions. This showed the hindering impact of wollastonite on the growth and deterioration of cotton ingredients. Similar hindering effects were previously reported on other cellulose-based materials, like particleboard (Taghiyari et al. 2014a), medium-density fiberboard (Taghiyari et al. 2014b), solid wood species (Karimi et al. 2013), and even paper (Taghiyari et al. 2014c).

Figure 3 Weight changes (a), tensile stress (b), and tensile stress (c) values in the control and NW-impregnated cotton cloth specimens in warp and wept directions, exposed to A. nigra for three months (capital letters on each column represent Duncan’s groupings). 

Control specimens in the warp direction and fungus-exposed specimens in wept direction had the highest (29.7 MPa) and the lowest (16.2 MPa) tensile stresses, respectively (Fig. 3b). Maximum tensile stresses in warp direction of the cotton fabric were higher in all treatments. This indicated that threads in the longitudinal direction of cotton textile had higher loading capacity than in the wept direction. With due consideration to the fact that in practice, different fabrics should usually bear more loading in the longitudinal direction in comparison to the transverse direction, this seems logical. Exposure to A. niger resulted in significant loss in tensile stress values in both warp and wept directions. The percentage of loss in warp and wept directions were 47% and 60%, respectively. The amount of losses in the NW-impregnated specimens were significantly lower; only about 7% and 4% in the warp and wept specimens, respectively.

As to the tensile strain values, wept specimens demonstrated higher strain values in all treatments. The highest and lowest strains were found in the NW-impregnated fungus-exposed specimens in wept direction (28.7%) and fungus-exposed specimens in the warp direction (16.7%), respectively (Fig. 3c). Exposure to A. nigra significantly decreased strain values in both directions. However, exposure to A. nigra in the NW-impregnated specimens did not result in any change in the strain values of either directions; the values even seemed to increase, though the increase was not statistically significant. This slight increase, and lack of evidence in decreasing values in tensile stress values (Fig. 3b), could be attributed to formation of extra bonds between cellulose between the compounds in cotton cloth and NW ingredients (Fig. 4) (Taghiyari et al. 2016). Density functional theory (DFT) demonstrated that the optimal adsorption distance and adsorption energy for NW of 1.7 Å and -6.6 eV, respectively. This rather large amount of energy is indicative of extra bonds formed between the Calcium atoms (in NW ingredients) and the Oxygen atoms (in the hydroxyl groups of the cellulose chains in cotton cloth) (Fig. 4) (Taghiyari et al. 2016). This eventually contributed to a higher tensile stain in NW-impregnated values; it also contributed to the slight decrease in the tensile stress values in the NW-impregnated fungus-exposed specimens in comparison to the fungus-exposed specimens. The formation of similar bonds was the main cause in improved physical and mechanical properties of different cellulose-based composites reported in some previous scientific projects.

Figure 4 Schematic representation of bond formation between Calcium atoms of NW and Oxygen atoms of cell-wall cellulose chains (Taghiyari et al. 2016). 

Cluster analysis based on the properties measured demonstrated that control specimens in both warp and wept directions were significantly clustered differently from those specimens exposed to A. nigra for three months (Fig. 5). However, the specimens that were impregnated with NW prior to fungus-exposure were closely clustered to the control treatments. This indicated that the hindering effects of NW on growth of the fungus were so effective that the overall properties of NW-impregnated specimens can be considered similar to those of the control specimens from a statistical point of view. It was therefore concluded that NW can be considered an efficient material to hinder the growth of A. nigra on cotton fabrics in order to maintain its strength. As to the non-toxic nature of wollastonite to both human and wildlife, it is also a safe and hygiene material to be environmentally friendly be used for different fabrics. However, further studies should be carried out to practically find out the ultimate amount of wollastonite to be used for each fabric, its effects on other fungal species, and many other aspects of its utilization.

Figure 5 Cluster analysis of the six treatments of cotton fabric specimens based on tensile strain and tensile stress values before and after exposure to Aspergillus niger fungus for three months (FE = fungal-exposed; NW = nano-wollastonite).  

CONCLUSIONS

Aspergillus niger is a constant threat to works of art in museums around the world. Therefore, the present research project focused on the effects of exposure of A. niger for three months on cotton textile in warp and wept directions. Nano-wollastonite (NW) was used to find out its protective effects on the growth and activities of the fungus. The results were indicative of a significant loss in tensile stress, demonstrating its high activity on cotton. However, its activity was significantly limited by prior-impregnation of the specimens with NW. It was concluded that NW positively protect cotton textile against A. nigra; however, complementary studies should be committed to firmly make decision on the ultimate NW-content.

ACKNOWLEGMENTS

The authors appreciate constant scientific support of Dr. Jack Norton (Retired, Horticulture and Forestry Science, Queensland Department of Agriculture, Forestry and Fisheries, Australia).

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Received: November 15, 2017; Accepted: December 20, 2017

Correspondence to: Hamid Reza Taghiyari E-mail: htaghiyari@yahoo.com

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