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Arquivos Brasileiros de Oftalmologia

Print version ISSN 0004-2749On-line version ISSN 1678-2925

Arq. Bras. Oftalmol. vol.82 no.1 São Paulo Jan./Feb. 2019  Epub Nov 01, 2018

http://dx.doi.org/10.5935/0004-2749.20190001 

ORIGINAL ARTICLE

Correlation between pathogenic species and clinical findings, disease severity, and visual outcome in patients with fungal keratitis

Correlação entre espécies patogênicas e achados clínicos, gravidade da doença e desfecho visual em pacientes com ceratite fúngica

Leidiane Adrinano Pereira1 

Rosália Antunes Foschini1 

Sidney Júlio de Faria e Sousa1 

1Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto, Universidade Estadual de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT

Purpose:

To determine whether a correlation exists between pathogenic species and clinical findings, disease severity, and visual outcome in patients with keratitis and fungal growth in microbiological culture.

Methods:

A retrospective study of patients with fungal growth in the microbiological culture of corneal scrapings. Patients were treated at an ophthalmologic reference center in Southeastern Brazil from January 1992 to October 2015.

Results:

Medical records of 181 patients (131 males and 50 females) with a mean age of 47 ± 18 years were analyzed. The three most common etiologies were Fusarium sp. (38.7%), Aspergillus sp. (15%), and Candida sp. (13.2%). Among these, Fusarium sp. was the most frequent in patients aged £50 years (p=0.002) and in those with a recent history of a foreign body and/or ocular trauma (p=0.01). Candida sp. was the most frequent etiology in patients aged >50 years (p=0.002), in those with postoperative ocular surgery (p=0.002); in those with a previous ocular pathology (p=0.0007); and in immunodepressed patients (p=0.0004).

Conclusion:

Fusarium sp. was predominant in patients aged £50 years and those with a recent history of foreign body and/or ocular trauma, whereas Candida sp. was predominant in older adults, in those with a postoperative ocular surgery, in those with a previous ocular pathology, and in immunodepressed patients.

Keywords: Keratitis; Eye infections, fungal; Corneal ulcer; Risk factors

RESUMO

Objetivo:

Determinar se existe uma correlação entre espécies patogênicas e achados clínicos, gravidade da doença e resultado visual em pacientes com ceratite e crescimento de fungos em cultura microbiológica.

Métodos:

Estudo retrospectivo de pacientes com crescimento de fungos na cultura microbiológica de raspado de córnea. Os pacientes foram tratados em um centro de referência oftalmológica no Sudeste do Brasil de janeiro de 1992 a outubro de 2015.

Resultados:

Foram analisados registros médicos de 181 pacientes (131 homens e 50 mulheres) com idade média de 47 ± 18 anos. As três etiologias mais comuns foram Fusarium sp. (38,7%), Aspergillus sp. (15%) e Candida sp. (13,2%). Entre estas, Fusarium sp. foi a mais frequente em pacientes com idade £50 anos (p=0,002) e naqueles com história recente de corpo estranho e/ou trauma ocular (p=0,01). Candida sp. foi a etiologia mais frequente em pacientes com idade >50 anos (p=0,002), naqueles com cirurgia ocular pós-operatória (p=0,002); naqueles com patologia ocular prévia (p=0,0007); e em pacientes imunodeprimidos (p=0,0004).

Conclusão:

Fusarium sp. foi predominante em pacientes com idade £50 anos e naqueles com história recente de corpo estranho e/ou trauma ocular; enquanto Candida sp. foi predominante em adultos mais velhos, naqueles com cirurgia ocular pós-operatória, naqueles com patologia ocular prévia e em pacientes imunodeprimidos.

Descritores: Ceratite; Infecções oculares fúngicas; Úlcera da córnea; Fatores de risco

INTRODUCTION

Microbial keratitis is one of the most common causes of preventable blindness worldwide(1). Several studies published in the literature have reported that several classes of bacteria, fungi, viruses, and parasites as etiologic agents of corneal ulcers(1-5).

Fungal keratitis is typically characterized by a dry, raised lesion with feathered/hyphenated edges, in addition to satellite lesions and hypopyon(6).

Pathogens that are isolated in a microbiological culture of human corneas with keratomycosis are more frequently filamentous fungi, such as Fusarium sp. and Aspergillus sp(7,8).

Conventional methods for the diagnosis of fungal ke­ratitis include Gram, 10% potassium hydroxide monoxide, lactophenol cotton blue, Giemsa, or calcofluor white staining(9-11). Sabouraud dextrose agar is a culture medium that is widely used to isolate fungi(10).

Risk factors associated with a higher predisposition for fungal keratitis include ocular trauma, often with tree branches; the incorrect use and improper cleaning of contact lenses; preexisting ocular surface disorders, such as dry eye disease and herpetic keratitis; systemic diseases, such as decompensated diabetes mellitus and immunodepression; and prolonged use of antibiotics(12,13).

Mycotic keratitis presents an important aggravating factor in that it affects individuals with a low socioeconomic and cultural status, particularly rural workers, a population often affected by microbial keratitis in their work environment and who often do not have eye protection. In addition to this greater predisposition, patients often delay seeking treatment in a specialized center, which worsens the final visual acuity (VA) results(14). Despite high cost implications and poor visual outcomes following treatment, it does not attract as much attention as other eye conditions such as cataracts, refractive errors, and childhood blindness(15).

Fungal keratitis can lead to serious complications including endophthalmitis, perforation, and blindness, particularly in cases where patients are not well conducted. Ophthalmologists continue to seek the most effective treatment modalities for these patients(8).

Yeasts are thought to be associated with systemic immunosuppression, whereas filamentous fungi are most often present in cases of ocular trauma in individuals involved in agricultural activities(15). As previously observed, a larger infiltration leads to a poorer visual outcome and older age and males are more associated with a poor vision(16).

Few published studies have reported on correlations between the pathogenic species and clinical characteristics in fungal keratitis.

The present study was conducted to determine correlations between the pathogenic species and demographic data, clinical status, disease severity, and visual outcomes in patients with fungal keratitis who were treated at an ophthalmologic reference center in Southeastern Brazil.

METHODS

This study was approved by the local Ethics Committee following the principles of the Declaration of Helsinki.

This study was a retrospective survey of medical records of 181 patients treated at the Clinic’s Hospital of Ribeirão Preto, University of São Paulo, Southeastern Brazil, and patients who had fungal growth in an ocular material (corneal scrapings) cultivated in a microbiological culture medium were included from January 1992 to October 2015.

All corneal scrapings were removed using a sterile Kimura spatula following an application of anesthetic eye drops. Corneal scrapings were routinely seeded in the following culture media: chocolate and blood agar plates, Sabouraud agar, and thioglycollate broth.

The following information was extracted from the medical records: demographic and occupational data, culture results, predisposing factors for fungal keratitis, symptoms and their duration, corneal biomicroscopy findings, and VA after treatment.

The Access database (Microsoft Corporation, Seattle, Washington, USA) was used to record and store survey data.

Statistical analyses were performed using Student’s t test for continuous data and percentages or Fisher’s exact test for categorical data. p<0.05 was considered statistically significant.

RESULTS

Epidemiological results

Medical records of 181 [131 (72.4%) males and 50 (27.6%) females] patients with a mean age of 47 ± 18 years were analyzed.

The primary work activity reported by patients was agriculture (29.8%).

The three most frequent etiologies of fungal keratitis were Fusarium sp. (38.7%), Aspergillus sp. (15%), and Can­dida sp. (13.2%).

Candida sp. was most prevalent in patients aged >50 years (p=0.002), and Fusarium sp. had a higher predisposition in those aged £50 years (p=0.002).

Table 1 illustrates the etiology of fungal keratitis ( Fusarium sp., Aspergillus sp., and Candida sp.) and patients’ epidemiological characteristics.

Table 1 Patients' epidemiological characteristics: comparison between the three main etiologies of fungal keratitis 

Characteristic Fusarium sp. (%) n=70 Aspergillus sp. (%) n=27 Candida sp. (%) n=24 p-value Fisher's exact test
Female 16 (22.8) 10 (37.0) 11 (45.8) 0.070
Male 54 (77.1) 17 (62.9) 13 (54.2) 0.070
Age ≤50 years 48 (68.6) 13 (48.1) 07 (29.1) 0.002
Age >50 years 22 (31.4) 14 (51.8) 17 (70.8) 0.002
Rural workers 23 (32.8) 08 (29.6) 03 (12.5) 0.150

Clinical evaluation

Of the factors associated with fungal keratitis, 67 of 181 (37%) patients had a recent history of foreign body (most often cited as dust) and/or ocular trauma, 27 (15%) had an ocular pathology history (e.g., glaucoma, dry eye, bullous keratopathy, recurrent herpes, diabetic retinopathy, or keratoconus), 26 (14.4%) had a recent ocular surgery (e.g., cataract surgery, corneal/conjunctival surgery, vitrectomy, or corneal transplantation), and 12 (6.6%) were immunoexpressed (e.g., acquired immunodeficiency syndrome, decompensated diabetes mellitus, chemotherapy, or use of systemic immunosuppressants).

Fusarium sp. had a higher prevalence in patients with a recent history of foreign body and/or ocular trauma (p=0.01), whereas Candida sp. was more frequently observed in patients with a recent ocular surgery (p=0.002), in those with previous ocular pathology (p=0.0007), and in immunosuppressed patients (p=0.0004).

Table 2 describes the etiology of fungal keratitis in asso­ciation with patients’ clinical aspects.

Table 2 Comparison between the three main etiologies of fungal keratitis and the frequency of associated factors 

Associated factor Fusarium sp. (%) n=70 Aspergillus sp. (%) n=27 Candida sp. (%) n=24 p-value Fisher's exact test
Foreign body and/or ocular trauma 27 (38.6) 7 (25.9) 02 (08.3) 0.0100
Postoperative ocular surgery 04 (05.7) 4 (14.8) 10 (41.7) 0.0002
Previous ocular pathology 03 (04.3) 7 (25.9) 07 (29.2) 0.0007
Immunodepression 01 (01.4) 4 (14.8) 05 (20.8) 0.0004

Ocular symptoms were described in 164 (90.6%) of 181 medical records. Ocular pain appeared in 108 (65.8%) records, and 65 (39.6%) patients complained of foreign body sensation. Patients presented with ocular symptoms within a mean period of 16.0 ± 21.6 days. The time to symptom onset was not identified in 29 records.

Table 3 demonstrates the etiology of fungal keratitis and patients’ ocular symptoms. There was no statistical difference between the etiology of fungal keratitis and patients’ symptoms at the first consultation.

Table 3 Patients' ocular symptoms: comparison between the three main etiologies of fungal keratitis 

Ocular symptom Fusarium sp. (%) n=66 Aspergillus sp. (%) n=23 Candida sp.(%) n=20 p-value Fisher's exact test
Pain 45 (68.2) 14 (60.9) 17 (85) 0.2
Foreign body sensation 27 (40.9) 10 (43.5) 06 (30) 0.7

The mean size of the largest ulcer diameter at the first visit, measured using biomicroscopy examination, was 4.0 ± 1.9 mm (data annotated in 92 cases). Twelve (6.6%) of 181 presented melting, 18 (10%) had descemetocele or corneal perforation, and 33 (18.2%) had hypopyon formation. There was no statistical difference between the etiology of fungal keratitis and biomicroscopic findings at the first consultation (Table 4).

Table 4 Comparison between the three main etiologies of fungal keratitis and biomicroscopy examination at the first visit 

Biomicroscopic finding Fusarium sp. (%) n=68 Aspergillus sp. (%) n=27 Candida sp. (%) n=24 p-value Fisher's exact test
Melting 09 (13.2) 0 1 (04.2) 0.09
Descemetocele/corneal perforation 05 (07.3) 4 (14.8) 5 (20.8) 0.16
Hypopyon 15 (22.0) 5 (18.5) 3 (12.5) 0.63

Treatment evaluation

In total, ulcer was cured in 28 (15.4%) patients using antibiotic eye drops and without using antifungal medication despite the laboratory result of fungal growth in culture. However, bacterial growth associated with fungal growth was identified in the microbiological culture in 24 (85.7%) of 28 patients, thereby illustrating the response to antibiotic treatment.

Ulcer healing was observed in 9 (17.3%) patients who were administered antiherpetic medication associated with antifungal treatment. Therefore, in these patients, a secondary fungal infection in the herpetic ulcers was assumed.

The mean time of antifungal treatment was 51.0 ± 33.5 days, and the mean treatment time did not differ among Candida sp ., Fusarium sp., or Aspergillus sp.

Visual acuity after treatment

The final VA could not be obtained in 137 (75.7%) of 181 medical records, and it was >0.5 (logMAR <0.3) in 27 (19.7%), between 0.2 and 0.5 (logMAR 0.7-0.3) in 22 (16.0%), and £0.1 (logMAR ≥0.8) in 88 (64.2%) patients.

Table 5 shows the final VA after fungal keratitis treatment. The final VA was not statistically different among the three most frequent etiologies.

Table 5 Patients' visual acuity (VA) after treatment: comparison between the three main etiologies of fungal keratitis 

VA after treatment Fusarium sp. (%) n= 55 Aspergillus sp. (%) n=22 Candida sp. (%) n=18 p-value Fisher's exact test
>0.5 (logMAR <0.3) 12 (21.8) 03 (13.6) 2 (11.1) 0.47
0.2-0.5 (logMAR 0.7-0.3) 10 (18.2) 02 (09.0) 2 (11.1) 0.67
≤0.1 (logMAR ≥0.8) 33 (60.0) 17 (77.3) 14 (77.8) 0.22

DISCUSSION

As described in other studies, the most prevalent ocular mycoses occur in men, often in rural workers, which is possibly attributable to their increased occupational exposure(11,17-20). In the present study, we showed a higher frequency of fungal ulcers in male patients (72.4%), rural workers (29.8%), and in those with a history of foreign body (more frequently cited as dust) and/or ocular trauma (37%). These workers often lack eye protection while working in the fields, which thereby puts them at risk for eye damage and consequent fungal keratitis.

The mean age of patients treated for fungal keratitis was 47 ± 18 years; therefore, they are in the economically active age group, a circumstance that has been already reported in previous studies as an epidemiological characteristic of fungal keratitis(17-21).

Ocular pain is a relevant symptom in patients with fungal keratitis (65.8%) and must be carefully evaluated by an ophthalmologist seeking clinical evidence for the diagnosis of a mycotic ulcer.

The mean time of symptoms reported at the first con­sultation was high (16.0 ± 21.6 days), a situation that compromises a patient’s visual recovery. A study conducted in London also found a high median range (3.4 weeks) from the onset of keratitis to the confirmation of fungal infection(22).

Following treatment, we observed a higher prevalence of final VA £0.1 (logMAR ³0.8) in 88 (64.2%) patients, independent of the fungal etiology. In addition, the final VA was not statistically different among the three most frequent etiologies. In addition, another study has found similar visual outcomes between patients with Candida infection and those with filamentous fungal infection(22).

Our laboratory results did not differ from published data in the literature, which have reported a higher pre­valence of fungal keratitis caused by Fusarium sp., Aspergillus sp. , Candida sp., among others(3,17,23-26).

In the present study, Fusarium sp. was found to be the most frequent etiology, particularly in patients aged £50 years, in addition to ocular trauma. A Brazilian study published in 2005 and a Danish study identified ocular trauma as a risk factor for filamentous fungal keratitis(27,28)). Researchers from South Florida in the U.S. have found that ocular trauma was particularly prevalent with infections caused by Fusarium sp(29). Similarly, a study in London also found that ocular trauma is a major risk factor for filamentous fungal infection(22).

This study demonstrated that Candida sp. is more frequent in patients with recent ocular surgery, previous ocular pathology, and immunosuppression. In addition, other studies have found that ocular surgeries and previous eye diseases are associated with yeast keratitis(22,27,28).

Data extracted from medical records revealed 28 patients whose ulcer was cured without using antifungal medication despite fungal growth in microbiological scrapings. This situation can be characterized according to information from previous studies showing a possible discrepancy between microbiology results and patients’ clinical findings as detailed below.

1) False-positive cultures: (I) when fungus coloniza­tion is identified in the patient, (II) when there is fungal contamination in the laboratory, (III) when the sample comes from two different areas, or (IV) when the pathological specimen is not adequately analyzed(29). This shows that clinical and microbiological findings should be evaluated together.

2) False-negative cultures: (I) when the tissue in the microbiology laboratory is crushed and fungal cells are destroyed, (II) when the fungus in the tissue is not viable, or (III) when the sample comes from two different areas(30). These conditions did not occur in our study because our starting point was patients with corneal ulcers and positive fungal cultures.

Because the starting point for our study was microbiological growth data of fungi in ocular material culture, cases of fungal keratitis without culture growth were not included. There is a technical difficulty in confirming fungal growth in microbiological cultures, which occurs within 3-4 days, but culture media may require up to 4-6 weeks of incubation(8).

Because our study was retrospective, some difficulties were encountered with data collection. For example, information on symptoms and disease time before treatment at the corneal outpatient clinic were not found in some cases, whereas in others, there was no description of ulcer size and VA information after treatment.

In conclusion, this study presented an impressive sample of cases of fungal keratitis that were sufficiently diagnosed using microbiological cultures. In addition, the study identified clinical features associated with the three major pathogenic species of serious ocular disease, in which an early diagnosis remains a challenge in most ophthalmological services, particularly in the developing countries.

Funding: No specific financial support was available for this study.

Approved by the following research ethics committee: Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (#1.452.814).

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Received: October 10, 2017; Accepted: June 03, 2018

Corresponding author: Leidiane Adriano Pereira. Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço. Av. Bandeirantes, 3.900 - Ribeirão Preto, SP - 14049-900 - Brazil E-mail: leidianeadriano@usp.br

Disclosure of potential conflicts of interest: None of the authors have any potential conflicts of interest to disclose.

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