Dear Editor:
I have recently read an article in your journal by Zhuang et al.(11 Zhuang H, Zheng NX, Lin L, Zhang WZ, Zhang WY, Yu QQ, et al. Fluorofenidone inhibits epithelial-mesenchymal transition in human lens epithelial cell line FHL 124: a promising therapeutic strategy against posterior capsular opacification. Arq Bras Oftalmol. 2021:S0004-27492021005001227. doi: 10.5935/0004-2749.20210040.
https://doi.org/10.5935/0004-2749.202100...
) and became interested in this work, specifically because as indicated in the title, the lens cell line FHL124 was used as an experimental model. I was curious to read in the Methods section that the authors obtained these cells from American Type Culture Collection (ATCC). As far as I am aware, FHL124 cells are not commercially available. Upon accessing the ATCC website and searching for lens cells, only human lens epithelial (HLE-B3) are available for sale. Indeed, the latter are virally transformed, whereas FHL124 cells are spontaneously transformed and better reflect observations in tissue culture models(22 Wormstone IM, Eldred JA. Experimental models for posterior capsule opacification research. Exp Eye Res. 2016;142(1):2-12.). Therefore, the authors should confirm that the cells they have used are indeed FHL124 and not HLE-B3 and, if so, clearly present the source of their FHL124 cells.
Kind regards,
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Funding: This study received no specific financial support.
REFERENCES
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1Zhuang H, Zheng NX, Lin L, Zhang WZ, Zhang WY, Yu QQ, et al. Fluorofenidone inhibits epithelial-mesenchymal transition in human lens epithelial cell line FHL 124: a promising therapeutic strategy against posterior capsular opacification. Arq Bras Oftalmol. 2021:S0004-27492021005001227. doi: 10.5935/0004-2749.20210040.
» https://doi.org/10.5935/0004-2749.20210040 -
2Wormstone IM, Eldred JA. Experimental models for posterior capsule opacification research. Exp Eye Res. 2016;142(1):2-12.
Publication Dates
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Publication in this collection
21 May 2021 -
Date of issue
May-Jun 2021
History
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Received
12 Feb 2021 -
Accepted
19 Feb 2021