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Arquivos de Gastroenterologia

Print version ISSN 0004-2803On-line version ISSN 1678-4219

Arq. Gastroenterol. vol.56 no.2 São Paulo Apr./June 2019  Epub Aug 26, 2019

https://doi.org/10.1590/s0004-2803.201900000-34 

ORIGINAL ARTICLE

ADIPONECTIN, VITAMIN D AND NUTRITIONAL STATUS IN PATIENTS WITH ADVANCED COLORECTAL CANCER OR DURING FOLLOW-UP

Adiponectina, vitamina D e estado nutricional em pacientes com câncer colorretal avançado e em seguimento

Mariana Abe Vicente CAVAGNARI1 
http://orcid.org/0000-0003-4352-4659

Verônica Marques VIDIGAL1 
http://orcid.org/0000-0002-4062-0015

Tiago Donizetti SILVA1 
http://orcid.org/0000-0002-0274-3604

Katia BARÃO1 
http://orcid.org/0000-0001-9975-4109

Nora Manoukian FORONES1 
http://orcid.org/0000-0001-9414-0343

1Universidade Federal de São Paulo (UNIFESP), Setor de Oncologia, Disciplina de Gastroenterologia Clínica, Departamento de Medicina, São Paulo, SP, Brasil.


ABSTRACT

BACKGROUND:

Considering the high incidence of colorectal cancer (CRC) related deaths, many studies have investigated variables that can affect survival, with the aim of prolonging survival. The nutritional status can also be predict survival in patients with CRC.

OBJECTIVE:

The aim of the present study was to evaluate if BMI, %FAT, PhA, PG-SGA, adiponectin levels, and vitamin D levels are relevant to the characterization and differentiation of patients with advanced CRC and patients with a history of CRC.

METHODS:

The study was carried out by patients with advanced colorectal cancer (Group 1) and patients in follow-up after colorectal cancer treatment (Group 2). Nutritional status was assessed using the body mass index, body fat percentage, phase angle from bioelectrical impedance, Patient-Generated Subjective Global Assessment score. Adiponectin concentrations were determined using an enzyme-linked immunosorbent assay, and vitamin D levels were measured using high performance liquid chromatography.

RESULTS:

Groups 1 and 2 consisted of 23 and 27 patients, respectively. The body mass index, body fat percentage, phase angle, vitamin D and adiponectin levels were not significantly different between the groups. The mean Patient-Generated Subjective Global Assessment score was significantly higher in group 1 compared with group 2, and was significantly correlated with the long-term mortality risk.

CONCLUSION:

Among the nutritional status parameters, only the Patient-Generated Subjective Global Assessment score was significantly different between the groups and was an important predictor of survival in patients with advanced colorectal cancer.

HEADINGS: Neoplasm metastasis; Colorectal neoplasms; Obesity; Body mass index; Adiponectin; Vitamin D

RESUMO

CONTEXTO:

Considerando a alta incidência de óbitos devido ao câncer coloretal (CCR), estudos investigaram variáveis que podem afetar a sobrevida, com objetivo de prolongar a sobrevida. O estado nutricional desses pacientes também pode predizer a sobrevida.

OBJETIVO:

O objetivo do presente estudo foi avaliar se o índice de massa corporal (IMC), a porcentagem de gordura, os níveis séricos de adiponectina e de vitamina D são relevantes para a caracterização e diferenciação de pacientes com CCR avançado e pacientes com histórico de CCR.

MÉTODOS:

O estudo foi realizado por pacientes com câncer colorretal avançado (Grupo 1) e pacientes em acompanhamento após o tratamento do CCR (Grupo 2). O estado nutricional foi avaliado por meio do IMC, percentual de gordura corporal, ângulo de fase da bioimpedância elétrica, escore de Avaliação Global Subjetiva Gerada pelo Paciente. As concentrações de adiponectina foram determinadas por ELISA e os níveis de vitamina D foram medidos por meio de cromatografia líquida de alta performance.

RESULTADOS:

Os grupos 1 e 2 consistiram de 23 e 27 pacientes, respectivamente. O IMC, percentual de gordura corporal, ângulo de fase, níveis de vitamina D e adiponectina não foram significativamente diferentes entre os grupos. O escore médio da Avaliação Global Subjetiva Gerada pelo Paciente foi significativamente maior no grupo 1 em comparação com o Grupo 2, e foi significativamente correlacionado com o risco de mortalidade a longo prazo.

CONCLUSÃO:

Entre os indicadores do estado nutricional, apenas o escore da Avaliação Global Subjetiva Gerada pelo Paciente foi significativamente diferente entre os grupos e foi um importante preditor de sobrevida em pacientes com câncer colorretal avançado.

DESCRITORES: Metástase neoplásica; Neoplasias colorretais; Obesidade; Índice de massa corporal; Adiponectina; Vitamina D

INTRODUCTION

More than 1.2 million new cases of colorectal cancer (CRC) are reported annually, resulting in 600,000 deaths. CRC has become the third most common cancer in the world, making it the fourth leading cause of cancer mortality1.

Considering the high incidence of CRC-related deaths, a number of studies have investigated factors that affect, or are associated with, mortality, with the aim of prolonging the survival of patients with CRC. The findings of several studies indicate that nutritional status can predict survival in these patients2-4.

Obesity, which is often assessed using body mass index (BMI) or percentage body fat (%FAT), is an important nutritional status factor associated with survival in CRC4. Baade et al.5 and Kuiper et al.6 reported that overweight patients with CRC had 25% and 55% increased CRC-specific mortality rates, respectively. Conversely, other studies have reported no difference in the risk of mortality between overweight and normal weight patients with CRC7,8.

Bioelectrical impedance analysis (BIA) provides early predictions of nutritional status by calculating the change in the cell membrane and fluid imbalances. The phase angle (PhA), which is one component of the BIA, determines cellular health and nutritional status by calculating the resistance of body fluid and reactance of the cellular membrane. A high PhA score indicates a good cellular membrane function, while a low PhA is closely associated with cell apoptosis and decreased extracellular matrix compounds9. In previous studies using BIA, the prognostic value of PhA was demonstrated in pancreatic cancer, lung cancer, breast cancer, and CRC10-12. In addition, a PhA of <50% of the standard PhA was associated with a decreased physical function and nutritional status, and an increased mortality rate in a previous study13.

The Patient-Generated Subjective Global Assessment (PG-SGA)14, recommended by the Oncology Nutrition Dietetic Practice Group of the American Academy of Nutrition and Dietetics15, is a standard nutritional assessment tool for cancer patients. It emphasizes symptoms commonly observed during the treatment of cancer and includes a physical examination for the subjective assessment of nutritional status. The PG-SGA is an important tool that predicts survival16, and patient-reported outcomes are particularly important for survival prediction17.

Adiponectin is a 30-kDa protein hormone and cytokine that is secreted mainly by adipocytes, is structurally related to the collagen superfamily, and shares homologies with collagens, complement factors, and tumor necrosis factor-α18. It is the most abundant hormone secreted by adipose tissue and circulates as low molecular weight and high molecular weight multimers18. Epidemiological studies have suggested that circulating adiponectin levels are inversely correlated with the risk of obesity-associated CRC, and accumulating evidence supports an inverse relationship between circulating adiponectin levels and obesity, suggesting that adiponectin may mediate the biological link between obesity and CRC19-21. In addition, higher levels of adiponectin receptor expression are observed in CRC than in normal tissues, further supporting this hypothesis21. However, there is a paucity of data on the effect of adiponectin on survival among patients with established CRC.

The vitamin D hypothesis has received strong experimental support over the past two decades, based on the almost ubiquitous expression in colon cancer cells of vitamin D receptors (VDR) 1,2 and hydroxylase,3, which convert plasma 25-hydroxyvitamin D3 [25(OH)D] into 1,25-dihydroxyvitamin D3 [1,25(OH)2D]. Binding of VDR by 1,25(OH)2D leads to multiple cellular effects, including the induction of differentiation and apoptosis22, and inhibition of proliferation, angiogenesis23, and metastatic potential24.

Prospective studies have demonstrated that higher baseline plasma 25(OH)D levels are associated with a significantly reduced risk of CRC25-26. Emerging evidence in the literature suggests an association between serum 25(OH)D levels and survival in CRC27,28, however, the relationship between vitamin D and CRC-related mortality is not well understood29.

Although the BMI, %FAT, PhA, PG-SGA score, and adiponectin and vitamin D levels have been correlated with survival in patients with CRC, their prognostic utility in patients with advanced cancer is not clear.

The aim of the present study was to evaluate whether the BMI, %FAT, PhA, PG-SGA score, and adiponectin and vitamin D levels are relevant for the characterization and differentiation of patients with advanced CRC compared with patients in follow-up following treatment for CRC. In addition, we aimed to determine whether these variables are important for the survival of patients with CRC.

METHODS

Subjects

This longitudinal study involved outpatients treated between February 2011 and March 2012 by the Oncology Group from the Gastroenterology Division of the Federal University of Sao Paulo, Brazil. The patients were evaluated in a single moment and the survival was evaluated until the year 2016. The study was approved by the local Ethics Committee (Protocol 0826/10 and 0029/11), and all patients signed an informed consent form. There were two groups of patients: group 1, which included patients with metastatic CRC undergoing chemotherapy; and group 2, which included patients in follow-up following treatment for CRC and who had been tumor free for >6 months.

Data collection

The sex, age, treatment, site, death, and tumor stage data were obtained from the patient medical records. The nutritional evaluation and blood sample collection to measure serum adiponectin and vitamin D levels were performed simultaneously. All patients were classified according to the tumor-node-metastasis staging system30. The BMI was calculated as weight (kg) divided by height (m2), and classified according to the World Health Organization criteria31. The PhA and %FAT were determined using the Biodynamics 450® analyzer and a standard protocol. PhA was calculated as the ratio between resistance (R) and reactance (Xc); R and Xc were measured directly in ohms at a single frequency of 50 kHz and 800 μA. All procedures and controls for other variables affecting the validity, reproducibility, and precision of the measurements were performed according to the National Institutes of Health guidelines32. The %FAT was classified according to the criteria by Gallagher et al.33.

The validated Portuguese version of the scored PG-SGA was used to assess nutritional status, which was classified as the following34: (A) well-nourished, (B) moderately undernourished or suspected of being undernourished, and (C) severely undernourished.

Adiponectin assay

Blood samples were collected in the morning after overnight fasting. The serum samples were clotted and centrifuged at 2000×g for 10 min and immediately frozen at -80°C for further analysis. Adipocytokine levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, MN, USA), and adiponectin levels (with the sample diluted 4,000-fold) were measured using the DuoSet® ELISA kit (DY1065; R&D Systems). The analytical methodology and technical procedures were performed according to the manufacturer’s protocol.

Vitamin D assay

Current and active vitamin D concentrations were measured using appropriate high performance liquid chromatography equipment, which was calibrated using a standard curve, analytical standards, and controls. The standard was provided by Sigma-Aldrich (St. Louis, MO, USA; C9774), the control was provided by Chromsystems (25-OH-Vitamin D3 serum control, Bi-level I + II), and the calibration curves were validated daily, with minor acceptable deviations of 5%. Measurement of samples was repeated when the values were lower or higher than the reference range (20-120 ng/mL).

The average circulating serum vitamin D concentration was classified as deficient (<20 ng/mL), insufficient (20-29.9 ng/mL), or sufficient (≥30 ng/mL). For the average active vitamin D concentrations, deficient was defined as <15 pg/mL, sufficient was defined as 15-78 pg/mL, and high was defined as >78 pg/mL.

Statistical analysis

Data are presented as percentages or the mean ± standard deviation. Differences between variables presented as percentages were assessed using chi-square tests. The normality of the results was tested using the Shapiro-Wilk test. The normally distributed continuous variables were assessed using Student’s independent t-tests and variables that non-parametric used Wilcoxon test.

To evaluate adiponectin levels and the PhA, a cutoff value was established for the population studied due to the lack of specific values for cancer patients. The values were divided by the distribution of the proportion of observed frequencies for both groups. The data were separated into quartiles, and the first quartile was defined as predictors of low levels and undernutrition for adiponectin and PhA, respectively. Survival analysis was conducted using the Kaplan-Meier method and evaluated using the log rank test. The SPSS 20.0 software (IBM Corp., Armonk, NY, USA) was used for statistical analyses. p < 0.05 was considered statistically significant.

RESULTS

Among the 50 patients who were eligible for the study, 23 were in group 1 and 27 were in group 2. Sex and age were not significantly different between the groups. The majority of group 2 had stage II tumors (51.8%). In both groups, the samples were collected predominantly during the winter. According PG-SGA, 47,8% and 25,9% patients were moderately undernourished, respectively in group 1 and 2 (Table 1).

TABLE 1 Baseline characteristics of colorectal cancer patients (Group 1) and controls (Group 2). 

Parameters Group 1 Group 2 P-value
Age (years) 58.2±10.2 59.6±11.9 0.630
Sex
Male 12 (52.2) 15 (55.6) 0.855
Female 11 (47.8) 12 (44.4)
Stage
I 3 (11.1)
II 14 (51.8)
III 10 (37.0)
IV 23 (100)
Rated season
Summer 1 (4.3) 2 (7.4) <0.001
Autumn 9 (39.2) 6 (22.3)
Winter 10 (43.5) 12 (44.4)
Spring 3 (13.0) 7 (25.9)
BMI
<18.5 (undernourished) 3 (11.1) 0.256
18.5-24.9 (normal) 14 (60.9) 13(48.2)
25-29.9 (overweight) 6 (26.1) 8 (29.6)
≥30.0 (obese) 3 (13.0) 3 (11.1)
% Fat mass
Adequate 11 (47.8) 11 (40.7) 0.630
High fat mass 12 (52.1) 16 (59.2)
PhA
>First quartile 16 (69.5) 25 (92.6) 0.659
≤First quartile 7 (30.4) 2 (7.4)
PG-SGA
Severely undernourished 1 (4.4) 0.725
Moderately undernourished 11 (47.8) 7 (25.9)
Well nourished 11 (47.8) 20 (74.0)
Adiponectin
>First quartile 16 (72.7) 14 (51.8) 0.922
≤First quartile 6 (27.3) 13 (48.2)
1,25 (OH) D
Deficient / insufficient 3 (13.0) 4 (14.8%) 0.470
Sufficient 20 (86.9) 23 (85.2)
25 (OH) D
Deficient / insufficient 12 (52.1) 13 (52) 0.384
Sufficient 11 (47.9) 12 (48)

Data are presented as the mean ± standard deviation or n (%). BMI, body mass index.

The BMI, %FAT, PhA, and 25(OH)D, 1,25(OH)D, and adiponectin levels were not significantly different between the groups, although the values were generally lower in group 1. The mean PG-SGA score was higher in group 1, median 8 points compared with group 2, median 2 points (P=0.002) (Table 2).

TABLE 2 Mean, median, minimum and maximum values of BMI, % Fat mass, phase angle, Score PG-SGA, adiponectin, 1,25 (OH) D and 25 (OH) D. 

Variables Mean ±SD Median P -value
BMI (kg/m2)
G1 24.5±3.5 24.7 (18.5-31.3) 0.129*
G2 26.3±4.3 26.2 (17.8-39.1)
% Fat mass
G1 25.1±8.1 26.9 (6.5-41.9) 0.500*
G2 26.7±8.4 28.9 (12-43.0)
PhA
G1 5.8±1.1 5.7 (3.8-8.0) 0.101*
G2 6.3±1.0 6.4 (4.3-8.5)
Score PG-SGA
G1 9.7±7 9 (1-25) 0.002*
G2 4±4.8 2 (0-16)
Adiponectin (µg/mL)
G1 12.5±2.7 12.8 ± (5-15) 0.066**
G2 10.4±3.4 12.18 (2.5-14.2)
1.25 (OH) D (ng/mL)
G1 28.3±18.6 20.6 (11.5-87.9) 0.796**
G2 29.5±14 26.8 (11.8-66.9)
25 (OH) D (pg/mL)
G1 28.8±16.3 28 (6.3-68.7) 0.585*
G2 31.6±19.1 27.3 (7-86)

Group 1: G1, Group 2: G2; *t-tests; **Wilcoxon test. minimum-maximum.

In the crude Kaplan-Meier analysis, the BMI, %FAT, PhA, and 25(OH)D, 1,25(OH)D, and adiponectin levels were not correlated with the long-term CRC-specific mortality risk. Only the PG-SGA score was significantly (log rank P=0.031) correlated with the long-term mortality risk (Figure 1).

FIGURE 1 Colorectal cancer survival according to the BMI (A), %FAT (B), phase angle (C), PG-SGA score (D), and 25(OH)D (E), 1,25(OH)D (F), and adiponectin levels (G) of patients. BMI, body mass index; %FAT, body fat percentage; PG-SGA, Patient-Generated Subjective Global Assessment; 25(OH)D, 25-hydroxyvitamin D; 1,25(OH)D, 1,25-hydroxyvitamin D. 

DISCUSSION

In the present study, the majority of the parameters of nutritional status (BMI, %FAT, PhA, and serum adiponectin and vitamin D levels) did not differ significantly between patients with advanced CRC and those with previous CRC. However, the PG-SGA score differed significantly between the two groups of patients, and was significantly higher among the patients with advanced disease. The PG-SGA score also served as a prognostic factor; patients with severe malnutrition had lower survival rates.

An association between PG-SGA scores and mortality has been reported previously16, and patient-reported outcomes were shown to be important for predicting survival17. One hypothesis for the PG-SGA score representing the only difference between these groups was that it was the only parameter that was not directly influenced by obesity. Even overweight patients can have a high PG-SGA score as a result of weight loss, symptoms that interfere with food intake, changes in functional capacity, and the physical examination.

In the present study, PhA values were lower in the patients with advanced CRC than in the patients with a history of CRC, as described in previous studies12,13. However, a significant difference between the groups was not observed in this study. Those patients with a PhA value below the first quartile have a worse prognosis. One limitation of the interpretation of the PhA value as a prognostic index may be related to not only the analysis approach, but also the period.

The lack of significant differences in the BMI, %FAT, PhA, and adiponectin and vitamin D levels between the two groups in the present study may have been due to the similar percentages of overweight or obese patients in the two groups (approximately 40%). In the present study, an association between obesity and disease progression in CRC was not observed. Previous studies have demonstrated that being underweight is associated with an increased risk of death due to cancer progression-associated weight loss7,8.

Obesity, as indicated by the BMI and %FAT, might justify the low serum adiponectin and vitamin D levels. High BMI and %FAT are reportedly risk factors for CRC4-6; this is supported by the similarities in the variables between the groups in the present study. Regarding the risk of disease recurrence, a consensus regarding the association between insufficient serum vitamin D levels and an increased risk of recurrence has not been reached, although there is a tendency to replenish vitamin D in patients with CRC and low vitamin D levels.

Serum adiponectin levels are associated with obesity, the risk of CRC, and a poor prognosis20. In the present study, the serum adiponectin levels did not differ significantly between the two groups, and there was no correlation between the different adiponectin quartiles and survival; however, patients with adiponectin levels in the first quartile had lower median survival rates.

To date, studies have shown that the pleiotropic roles of adiponectin in carcinogenesis are complex and controversial20. Anti-carcinogenic properties for adiponectin have been attributed to the activation of the adenosine monophosphate-activated protein kinase (AMPK) pathway, leading to apoptosis and inhibition of proliferation18; direct inhibition of the phosphoinositide 3-kinase/protein kinase-B pathway, which is responsible for cell survival; and modulation of insulin sensitization and inflammation18,20,21. Conversely, adiponectin was shown to promote oncogenesis through stimulation of pro-inflammatory cytokines, such as interleukin-8, inhibition of apoptosis via activation of AMPK/Sirtuin 1/peroxisome proliferator-activated receptor gamma coactivator 1 alpha18 and promotion of angiogenesis, and colonic proliferation18,20,21.

Since adiponectin regulates signaling pathways that consequently affect serum levels, the similarity in adiposity between the groups was more striking than the presence of a tumor in the present study. However, the evidence relating to adiponectin in the present study might be limited by the small sample size, single dose, and analysis of the total adiponectin level, rather than high molecular weight adiponectin.

A better understanding of the relationship between the analyzed variables and CRC is required. In addition, monitoring of these variables is needed to assist clinicians with possible interventions and to estimate survival with improved accuracy. Despite the importance of this study, some limitations were present, including the sample size and that approximately 40% of the patients were overweight; therefore, sufficient power might not have been present to demonstrate differences in the variables. Furthermore, the cross-sectional design did not allow for evaluation of longitudinal changes.

PG-SGA is a tool considered important for nutritional diagnosis and also for patient survival. However, depending on the moment the patient is evaluated, PG-SGA will not be effective to correlate with survival17,35. It is observed that changes in body composition36, such as body fat in Figure 1B, can be related to the survival of the patient with CRC.

The comparisons of nutritional status parameters between the patients with advanced CRC and those who were undergoing follow-up showed no significant differences in the BMI, PhA, %FAT, and serum adiponectin and vitamin D levels between the two groups. The PG-SGA score was significantly different between the two groups and was an important predictive parameter for survival in patients with advanced CRC. We recommend that additional studies involving longitudinal monitoring of nutritional parameters be performed, in order to determine the sensitivity to changes at different time periods and to predict survival. This should allow the best treatment plan to be determined based on the nutritional status of a patient.

ACKNOWLEDGEMENTS

The authors gratefully acknowledge the Fundação de Amparo à Pesquisa do Estado de São Paulo for providing financial support (10/19191-2).

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Disclosure of funding: this study was supported by the São Paulo Research Foundation (FAPESP, grant 10/19191-2).

Received: February 25, 2019; Accepted: June 11, 2019

Corresponding author: Mariana Abe Vicente Cavagnari. E-mail: marianaav@hotmail.com

Authors’ contribution: Cavagnari MAV and Forones NM equally contributed to the conception and design of the research; Cavagnari MAV, Vidigal VM, Silva TD and Barão K contributed to the acquisition and analysis of the data; Cavagnari MAV and Forones NM contributed to the interpretation of the data; and Cavagnari MAV and Forones NM drafted the manuscript. All authors critically revised the manuscript, agree to be fully accountable for ensuring the integrity and accuracy of the work, and read and approved the final manuscript.

Declared conflict of interest of all authors: none

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