Metabolismo do cobre nas epilepsias

Assis, Luís Marques de

doi:10.1590/S0004-282X1966000400001

Resumos

Partindo do fato de que o metabolismo do cobre está alterado nas epilepsias, o autor estudou sob que forma essa alteração se manifesta e, mediante o uso de curare em pacientes mentais submetidos ao eletrochoque, a influência da hiperatividade muscular própria da crise convulsiva sobre o metabolismo do cobre. Além disso, o autor estudou as correlações entre sexo e idade dos pacientes epilépticos, de um lado, e cupremia e cuprorraquia, do outro, e fez a correlação entre os níveis de cobre do soro e do LCR desses pacientes. Nos pacientes epilépticos (84) foram colhidas amostras de sangue (81 casos) e de LCR (66 casos) em período intercrítico para dosagem do cobre total. Nos pacientes mentais (32) foram colhidas amostras de sangue antes e imediatamente após crise convulsiva determinada pelo eletrochoque simples (22 casos) para dosagem de ceruloplasmina, e imediatamente após eletrochoque sob ação de curare (10 casos) para dosagem do cobre total. Os resultados foram submetidos a análise estatística, tendo o autor chegado às seguintes conclusões: 1) o nível de cobre do sangue e do LCR dos pacientes epilépticos não depende do sexo nem da idade; 2) o teor de cobre do LCR está correlacionado com o do sangue nos pacientes epilépticos; 3) a supressão, mediante o uso de substância curarizante, das manifestações musculares da crise produzida pelo eletrochoque acarreta queda não significativa do nível de cobre do sangue; 4) a queda do nível de cobre do sangue após convulsões determinadas pelo eletrochoque depende da hiperatividade muscular que caracteriza a crise convulsiva; 5) a queda do nível de cobre do sangue após convulsões determinadas pelo eletrochoque depende principalmente do cobre de reação direta; 6) a queda dos níveis de cobre do sangue após convulsões determinadas pelo eletrochoque é devida à pas* sagem desse elemento para os espaços intracelulares.

Since copper metobolism is disturbed in the epilepsies, the author studied in what form this alterations occurs and, by means of curare-like drugs in mental patients submitted to electroshock, the role played by convulsive induced muscular hyperactivity on copper metabolism is analysed. The author studied also the correlations of sex and age with blood and cerebrospinal copper levels in the epileptic patients. Eight four epileptic patients were studied. Copper was determined in blood samples (81 cases) and. cerebrospinal fluid samples (66 cases). In the mental patients (32 cases) blood ceruloplasmin was determined (22 cases) just after electroshock and blood copper was determined (10 cases) just after electroshock under curare-like drugs. The results were submitted to the conventional statistical analysis and the following conclusions were drawn: 1) the blood and cerebrospinal fluid copper is not dependent on sex or age; 2) the cerebrospinal fluid copper is correlated to the blood copper levels in the epileptic patients; 3) with the supression of the electroshock induced convulsive muscular activity through curare-like drugs, the fall of blood copper content is not significant; 4) the fall of blood copper levels after the electroshock induced convulsion depends on the convulsive muscular hyperactivity; 5) the fall of the blood copper content after electroshock induced is due to the passage of direct reacting copper into cells.

Metabolismo do cobre nas epilepsias

Cooper metabolism in the epilepsies

Luís Marques de Assis

Médico assistente; Clínica Neurológica da Faculdade de Medicina da Universidade de São Paulo (Prof. Adherbal Tolosa)

ABSTRACT

Partindo do fato de que o metabolismo do cobre está alterado nas epilepsias, o autor estudou sob que forma essa alteração se manifesta e, mediante o uso de curare em pacientes mentais submetidos ao eletrochoque, a influência da hiperatividade muscular própria da crise convulsiva sobre o metabolismo do cobre. Além disso, o autor estudou as correlações entre sexo e idade dos pacientes epilépticos, de um lado, e cupremia e cuprorraquia, do outro, e fez a correlação entre os níveis de cobre do soro e do LCR desses pacientes.

Nos pacientes epilépticos (84) foram colhidas amostras de sangue (81 casos) e de LCR (66 casos) em período intercrítico para dosagem do cobre total. Nos pacientes mentais (32) foram colhidas amostras de sangue antes e imediatamente após crise convulsiva determinada pelo eletrochoque simples (22 casos) para dosagem de ceruloplasmina, e imediatamente após eletrochoque sob ação de curare (10 casos) para dosagem do cobre total.

Os resultados foram submetidos a análise estatística, tendo o autor chegado às seguintes conclusões: 1) o nível de cobre do sangue e do LCR dos pacientes epilépticos não depende do sexo nem da idade; 2) o teor de cobre do LCR está correlacionado com o do sangue nos pacientes epilépticos; 3) a supressão, mediante o uso de substância curarizante, das manifestações musculares da crise produzida pelo eletrochoque acarreta queda não significativa do nível de cobre do sangue; 4) a queda do nível de cobre do sangue após convulsões determinadas pelo eletrochoque depende da hiperatividade muscular que caracteriza a crise convulsiva; 5) a queda do nível de cobre do sangue após convulsões determinadas pelo eletrochoque depende principalmente do cobre de reação direta; 6) a queda dos níveis de cobre do sangue após convulsões determinadas pelo eletrochoque é devida à pas* sagem desse elemento para os espaços intracelulares.

ABSTRACT

Since copper metobolism is disturbed in the epilepsies, the author studied in what form this alterations occurs and, by means of curare-like drugs in mental patients submitted to electroshock, the role played by convulsive induced muscular hyperactivity on copper metabolism is analysed. The author studied also the correlations of sex and age with blood and cerebrospinal copper levels in the epileptic patients.

Eight four epileptic patients were studied. Copper was determined in blood samples (81 cases) and. cerebrospinal fluid samples (66 cases). In the mental patients (32 cases) blood ceruloplasmin was determined (22 cases) just after electroshock and blood copper was determined (10 cases) just after electroshock under curare-like drugs.

The results were submitted to the conventional statistical analysis and the following conclusions were drawn: 1) the blood and cerebrospinal fluid copper is not dependent on sex or age; 2) the cerebrospinal fluid copper is correlated to the blood copper levels in the epileptic patients; 3) with the supression of the electroshock induced convulsive muscular activity through curare-like drugs, the fall of blood copper content is not significant; 4) the fall of blood copper levels after the electroshock induced convulsion depends on the convulsive muscular hyperactivity; 5) the fall of the blood copper content after electroshock induced is due to the passage of direct reacting copper into cells.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

* As dosagens do cobre e da ceruloplasmina foram feitas pelo Dr. Francisco Bastos de Jorge, Médico-Chefe do Laboratório da 1.ª Clínica Médica.

** Agradecemos a orientação, na parte referente à análise estatística, dada pelo Livre-Tese de Doutoramento elaborada na Clínica Neurológica da Faculdade de Medicina da Universidade de São Paulo (Prof. Adherbal Tolosa)

Docente Rubens Murillo Marques, do Departamento de Estatística da Faculdade de Higiene e Saúde Pública.

Nota do autor: Agradecemos aos colegas e técnicos que, direta ou indiretamente, colaboraram para a feitura dêste trabalho.

Clínica Neurológica - Faculdade de Medicina da Universidade de São Paulo - Caixa Postal 3461 - São Paulo, SP. Brasil

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3. ANGEL, C.; LEACH, B. E.; MARTENS, S.; COHEN, M. & HEATH, R. G. - Serum oxidation tests in schizophrenic and normal subjects. Arch. Neurol. Psychiat. (Chicago). 78:500-504, 1957.
4. BAKWIN, R. M.; MOSBACH, E. H. & BAKWIN, H. - Ceruloplasmin activity in the serum of children with schizophrenia. Pediatrics. 22:905-909, 1958.
5. BAKWIN, R. M.; MOSBACH, E. H. & BAKWIN, H. - Concentration of cooper in serum of children with schizophrenia. Pediatrics. 27:642-644, 1961.
6. BOVI, A. & MASCELLANI, A. - Determinazione del tasso di rame serico negli schizofrenici. G. Psichiat. Neuropat. 86:163-173, 1958.
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8. CARTWRIGHT, G. E. & WINTROBE, M. M. - Copper metabolism in normal subjects. Amer. J. clin. Nutr. 14:224-232, 1964.
9. COSTA-FORU, D. - Modificarile cuprului din creier, ficat, rinichi, splina si singe determinate de accesul convulsiv experimental la sobolani. Stud. Cercet. Neurol. 6:125-129, 1961.
10. COSTA-FORU, D. - Influenta efortului fizic asupra nivelului cuprului sanguin in nevrosa astenica. Stud. Cercet. Neurol. 6:320-323, 1961.
11. DE JORGE, F. B. - Alguns Aspectos do Metabolismo do Cobre. Monografia publicada pelo Serviço de Documentação da Reitoria da Universidade de São Paulo, Cidade Universitária, São Paulo, 1965.
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15. DE JORGE, F. B.; DELASCIO, D. & ANTUNES, M. L. - Copper and copper oxidase concentrations in the blood serum of normal and pregnat women. Obstet. and Gynec. 26:225-227, 1965.
16. DU BUY, H. G. - Mitochondria in brain as the sites of its metabolic activity. Neurology (Minneapolis) 8, supl. 1:69-71, 1958.
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23. GEKHT, B. M. - Soderzhanie medi v sivorotke krovi v norme i u bolinich nekotorini zabolevaniyamis godovnogomozga (Resumo em francês: Le contenu de cuivre dans le serum sanguin dans la norme et chez les malades atteints de certaines affections cérébrales organiques). Zh. Nevropat. Psikhiat. 61:707-712, 1961.
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26. GRIFFITHS, D. E. & WHARTON, D. C. - Purification and properties of cytochrome oxidase. Bioch. biophys. Res. Comun. 4:151-155, 1961.
27. GUBLER, C. J. - Cooper metabolism in man. J. Amer. Med. Ass. 161:530-535, 1956.
28. GUBLER, C. J.; LAHEY, M. E.; CARTWRIGT, G. E. & WINTROBE, M. M. - Studies on copper metabolism. The transportation of copper in blood. J. clin. Invest. 32:405-414, 1953.
29. GUBLER, C. J.; CARTWRIGT, G. E. & WINTROBE, M. M. - Studies on copper metabolism: enzyme activities and iron metabolism in copper and iron deficiencies. J. biol. Chem. 224:533-546, 1957.
30. HEILMEYER, L.; KEIDERLING W. & STUWE, G. - Kupfer und Eisen als korpereigene Wirkstoffe und ihre Bedeutung bein Krankheitsgeschehen. G. Fischer, Yena, 1941.
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Datas de Publicação

Publicação nesta coleção
14 Ago 2013
Data do Fascículo
Dez 1966

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ABOOD, L. G.; GIBBS, F. A. & GIBBS, E. - Comparative study in blood ceruloplasmin in schizophrenia and other disorders. Arch. Neurol. Psychiat. (Chicago) 77:643-645, 1957.

[2] 2. ADELSTEIN, J. & VALLEE, B. L. - Cooper metabolism in man. New Engl. J. Med. 265:892-897 e 941-946, 1961.

[3] 3. ANGEL, C.; LEACH, B. E.; MARTENS, S.; COHEN, M. & HEATH, R. G. - Serum oxidation tests in schizophrenic and normal subjects. Arch. Neurol. Psychiat. (Chicago). 78:500-504, 1957.

[4] 4. BAKWIN, R. M.; MOSBACH, E. H. & BAKWIN, H. - Ceruloplasmin activity in the serum of children with schizophrenia. Pediatrics. 22:905-909, 1958.

[5] 5. BAKWIN, R. M.; MOSBACH, E. H. & BAKWIN, H. - Concentration of cooper in serum of children with schizophrenia. Pediatrics. 27:642-644, 1961.

[6] 6. BOVI, A. & MASCELLANI, A. - Determinazione del tasso di rame serico negli schizofrenici. G. Psichiat. Neuropat. 86:163-173, 1958.

[7] 7. CANELAS, H. M.; ASSIS, L. M.; DE JORGE, F. B.; TOLOSA, A. P. M & CINTRA, A.B.U. - Disorders of copper metabolism in epilepsy. Acta neurol. scand. 40:97-106, 1964.

[8] 8. CARTWRIGHT, G. E. & WINTROBE, M. M. - Copper metabolism in normal subjects. Amer. J. clin. Nutr. 14:224-232, 1964.

[9] 9. COSTA-FORU, D. - Modificarile cuprului din creier, ficat, rinichi, splina si singe determinate de accesul convulsiv experimental la sobolani. Stud. Cercet. Neurol. 6:125-129, 1961.

[10] 10. COSTA-FORU, D. - Influenta efortului fizic asupra nivelului cuprului sanguin in nevrosa astenica. Stud. Cercet. Neurol. 6:320-323, 1961.

[11] 11. DE JORGE, F. B. - Alguns Aspectos do Metabolismo do Cobre. Monografia publicada pelo Serviço de Documentação da Reitoria da Universidade de São Paulo, Cidade Universitária, São Paulo, 1965.

[12] 12. DE JORGE, F. B. & CANELAS, H. M. - Contribuição ao estudo da ceruloplasmina. Valôres normais no sôro sanguíneo. Arq. Neuro-Psiquiat. 22:271-276, 1964.

[13] 13. DE JORGE, F. B.; CANELAS, H. M. & COSTA-SILVA, A. - Contribuição ao estudo do metabolismo do cobre. Metodologia da determinação do cobre em materiais biológicos. Rev. paul. Med. 61:350-355, 1962.

[14] 14. DE JORGE, F. B.; CANELAS, H. M. & SPINA-FRANÇA, A. - Contribuição ao estudo do metabolismo do cobre. Valôres normais no sôro sanguíneo, líquido cefalorraqueano e urina. Rev. paul. Med. 62:125-128, 1963.

[15] 15. DE JORGE, F. B.; DELASCIO, D. & ANTUNES, M. L. - Copper and copper oxidase concentrations in the blood serum of normal and pregnat women. Obstet. and Gynec. 26:225-227, 1965.

[16] 16. DU BUY, H. G. - Mitochondria in brain as the sites of its metabolic activity. Neurology (Minneapolis) 8, supl. 1:69-71, 1958.

[17] 17. EARL, C. J. - Anatomical distribution of copper in human brain. In Walshe, J. M. & Cumings, J. N. - Wilson's Disease. Some Current Concepts. Blackwell Scientific Publications, Oxford, 1961, pp. 18-23.

[18] 18. EDELSTEIN, E. L.; KRASSILOWSKI, D. & GDALIA, J. - A study on serum copper level in 20 acute schizophrenics. J. ment. Sci. 105:511-513, 1959.

[19] 19. EICHEL, B.; WAINIO, W. W.; PERSON, P. & COOPERSTEIN, S. J. A partial separation and characterization of cytochrome oxidase and cytochrome b. J. biol. Chem. 183:89-103, 1950.

[20] 20. ESCOBAR, A. I. & NIETO, D. - The role of copper in mental disorders. Bol. Inst. Estud. méd. biol. Mexico. 15:159-170, 1957.

[21] 21. FERRONI, A. & INDOVINA, I. - Variazioni della cupremia consecutiva ad elettroshock ed eletroassenza. Acta neurol. (Napoli). 5:291-299, 1950.

[22] 22. FROHMAN, C. E.; GOODMAN, M.; LUBY, E. D.; BECKETT, P.G:S. & SENF, R. - Ceruloplasmin, transferrin and tryptophan in schizophrenia. Arch. Neurol. Psychiat. (Chicago). 79:730-734, 1958.

[23] 23. GEKHT, B. M. - Soderzhanie medi v sivorotke krovi v norme i u bolinich nekotorini zabolevaniyamis godovnogomozga (Resumo em francês: Le contenu de cuivre dans le serum sanguin dans la norme et chez les malades atteints de certaines affections cérébrales organiques). Zh. Nevropat. Psikhiat. 61:707-712, 1961.

[24] 24. GOODMAN, L. S. & GILMAN, A. - The Pharmacological Basis of Therapeutics. The Mac Millan Co., New York, 1955, pp. 1468-1472.

[25] 25. GRASHCHENKOV, N. I. & GEKHT, B. M. - Cooper content of brain tissues in health and in certain nervous disseases. Exp. Neurol. 2: 573-580, 1960.

[26] 26. GRIFFITHS, D. E. & WHARTON, D. C. - Purification and properties of cytochrome oxidase. Bioch. biophys. Res. Comun. 4:151-155, 1961.

[27] 27. GUBLER, C. J. - Cooper metabolism in man. J. Amer. Med. Ass. 161:530-535, 1956.

[28] 28. GUBLER, C. J.; LAHEY, M. E.; CARTWRIGT, G. E. & WINTROBE, M. M. - Studies on copper metabolism. The transportation of copper in blood. J. clin. Invest. 32:405-414, 1953.

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