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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Arq. Neuro-Psiquiatr. vol.24 no.4 São Paulo Dec. 1966 

Células binucleadas no núcleo do nervo hipoglosso humano através dos grupos etários


Binucleated nerve cells in the human nucleus n. hypoglossi through ages



Cecil José Rezze

Professor Assistente; Departamento de Anatomia Descritiva e Topográfica da Faculdade de Medicina da Universidade de São Paulo (Prof. Odorico Machado de Sousa)




Foram contadas e estudadas as células uni- e binucleadas do núcleo do nervo hipoglosso humano de 26 indivíduos brancos e do sexo masculino, com idades variando entre 4 meses a 86 anos, todos sem processos patológicos que presumivelmente determinassem alterações nas células nervosas. Os cortes seriados com 30µ de espessura, foram corados pelo método de Pal Weigert modificado por Erhart, sendo feita coloração de fundo pelo picrocarmim. Mediante comparação dos diâmetros medidos ortogonalmente foi verificado, pelo estudo estatístico, que as células binucleadas eram maiores que as uninucleadas.
O pequeno número de células binucleadas (33 em 202.910 células contadas), sua distribuição indiferente nos diversos grupos etários e a ausência de figuras de reprodução mitótica ou amitótica, levam à conclusão de que a sua presença não caracteriza um processo de involução senil.
O significado deste achado que tem sido atribuído a processo de divisão celular mitótica ou amitótica, à divisão nuclear ou nucleolar sem chegar à divisão citoplasmática ou à fusão de duas células uninucleadas, é discutido à luz dos dados constantes da literatura.


It has been generally accepted that mature neurons of the central nervous system of adult mammals are incapable of cell-division. Nevertheless, recent data are suggesting the contrary. Mature neurons, bi- or multinucleated, observed in the central nervous system of vertebrates, mammals and man are being differently interpreted: as a result of mitotic or amitotic cell-division of mature neurons; as indiferentiated cells which could indicate a regeneration process; as a cellular reaction to nervous injuries; as the result of the fusion between two mononucleated elements.
The cells of the human nucleus n. hypoglossi were counted an studied in 26 white males, the youngest four months old, the eldest, eightysix years old. The material secured at the necropsy table was selected in order to exclude diseases or physical conditions which could interfere with the final results.
The medulla oblongata were formalin fixed, imbedded in celloidin, 30
µ serial cross sectioned, numbered and stained by Pal-Weigert modified by Erhart method for myelin sheaths. The counterstain was by carmin.
The cells were counted when their cell-bodies showed evident nucleus and nucleolus. They were measured and carefully analysed including with high magnification.
From the 202.910 counted cells, 33 were binucleated. These latter did not present any characteristic of mitotic or amitotic cell-division an no relation between age and higher frequency of binucleated cells could be observed.
The general morphology of the binucleated cells was equivalent to the mononucleated ones although the former were significantly larger than the latter. The statistical analysis was made by comparision of sample mean of two populations at the five per cent level of significance.
It is concluded, considering the studied material and the current literature, that there is no reason to accept, the presence of binucleated nerve cells as a biological reaction due to old age. The real significance of these cells is still unknown, but it can be admitted that they should have a biological capacity which would be qualitatively the same in the differente ages. Nevertheless, their quantitative evaluation is not possible, as far as we know.



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Departamento de Anatomia (.Descritiva e Topográfica). Faculdade de Medicina da Universidade de São Paulo. Caixa Postal 2921. São Paulo SP - Brasil

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