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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Arq. Neuro-Psiquiatr. vol.28 no.2 São Paulo June 1970

http://dx.doi.org/10.1590/S0004-282X1970000200001 

Histochemical observations of fluorescent biogenic amines in cryostat sections of peripheral and central nervous tissue

 

Verificação histoquímica de aminas biogênicas fluorescentes em secções criostáticas de tecido nervoso central e periférico

 

 

O. Duarte-Escalante; J. P. Pickett; R. E. Pendergrass

Department of Psychiatry and Department of Pathology, Duke University Medical Center, Durham, North Carolina

 

 


RESUMO

Relato de modificação da técnica criostático-histoquímica para a verificação da fluorescência das catecolaminas e da serotonina em secções de tecido nervoso central e periférico. São discutidas as vantagens desta modificação técnica em relação a outras propostas para a mesma finalidade.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

REFERENCES

1. CORRODI, H. & JONSSON, G. — The formaldehyde fluorescence method for the histochemical demonstration of biogenic amines. A review on the methodology. J. Histochem. Cytochem. 15:65, 1967.         [ Links ]

2. CSILLIK, B. & KALMAN, G. — Vacuumless freezing-drying: its application in catecholamine histochemistry. Histochem. 9:275, 1967.         [ Links ]

3. DAHLSTRÖM, A. & FUXE, K. — Evidence for the existence of monoaminecontaning neurons in the central nervous system. Demonstration of monoamines in the cell bodies of brain stem neurons. Acta Physiol. Scand. 62 Suppl. 232, 1964.         [ Links ]

4. DUARTE-ESCALANTE, O.; LABAY, P. & BOYARSKY, S. — The neurohistochemistry of mammalian ureter: a new combination of histochemical procedures to demonstrate adrenergic, cholinergic and chromaffin structures in ureter. J. Urol. 101:803, 1969.         [ Links ]

5. ELLINWOOD, E. H., Jr. & DUARTE-ESCALANTE, O. — Behavior and histopathological findings during chronic methedrine intoxication. J. Biol. Psychiat. (in press).         [ Links ]

6. ERÄNKO, O. — Histochemical demonstration of catecholamines by fluorescence induced by formaldehyde vapour. J. Histochem. Cytochem. 12:487, 1964.         [ Links ]

7. FALCK, M.; HILLARP, N. A.; THIEME, G. & TORP, A. — Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10:348, 1962.         [ Links ]

8. BAMBERGER, B.; MALMFORS, T. & SACKS, C. — Standardization of paraformaldehyde and of certain procedures for the histochemical demonstration of catecholamines. J. Histochem. Cytochem. 13:147, 1965.         [ Links ]

9. HOKFELT, T. — A modification of the histochemical fluorescence method for the demonstration of catecholamines and 5-hydroxytryptamine, using Araldite as embedding medium. J. Histochem. Cytochem. 13:518, 1965.         [ Links ]

10. NELSON, J. S. & WAKEFIELD, P. L. — The cellular localization of catecholamines in frozen-dried cryostat sections of the brain and autonomic nervous system. J. Neuropath. Exp. Neurol. 27:221, 1968.         [ Links ]

11. PLACIDI, G. F. & MASUOKA, D. T. — Histochemical demonstration of fluorescent catecholamine terminals in cryostat sections of brain tissue. J. Histochem. Cytochem. 16:491, 1968.         [ Links ]

12. SPRIGGS, T. L. B.; LEVER, J. D.; REES, P. M. & GRAHAM, J. D. P. — Controlled formaldehyde-catecholamine condensation in cryostat sections to show adrenergic nerves by fluorescence. Stain Technol. 41:323, 1966.         [ Links ]

 

 

Mailing address: O. Duarte-Escalante, M. D. — Duke University Medical Center — Box 2922 — Durham, North Carolina 27706 — U.S.A.
The research reported in this paper was done while the senior author was supported in part by a Post-Doctoral Research Training Fellowship from the National Institute of Mental Health, Grant No. 5tl-MH 8394-02 and 2F11 NB 2006-02 NSRA at the Department of Psychiatry and Department of Pathology, Duke University Medical Center, Durham, North Carolina.

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