Proteínas totais do liquido cefalorraqueano obtido por punção da cisterna magna: valores normais. Variações ligadas ao sexo

Vermes, L. M. Singer; Ferri, Rubens Guimarães; Aisen, J.; Marlet, J. M.

doi:10.1590/S0004-282X1976000400001

Resumos

Aplicando o método de Lowry, determinamos as proteínas totais do LCR considerado normal de 98 pessoas: 58 do sexo masculino e 40 do feminino. Todas as amostras de LCR foram obtidas por punção da cisterna magna (LCR-SO). Mediante tratamento estatístico dos valores obtidos, estabelecemos as taxas normais de proteinorraquia cisternal para a população mista: limite superior de 40,36 mg/100 ml, valor médio de 26,78 mg/100 e limite inferior de 13,20 mg/100 ml. Estes valores são superiores àqueles referidos na literatura. Foi verificado que a proteinorraquia total é, em média, significantemente maior nos indivíduos do sexo masculino, que nos de feminino. Consequentemente estabelecemos em separado os níveis normais de proteínas totais do LCR-SO para pessoas do sexo masculino e feminino. As taxas médias de proteínas totais do LCR-SO de pessoas do sexo masculino e feminino são de 28,76 e 23,91 mg/100 ml, respectivamente; os limites normais vão de 16,96 a 47,13 mg/100 ml para o sexo masculino e de 14,76 a 42,76 mg/100 ml para o sexo feminino.

The total protein content of CSF collected from the cisterna magna (cisternal fluid) of 98 patients (58 males and 40 females) with no neurological diseases was determined by the colorimetric method devised by Lowry and co-workers. This method has been previously chosen based mainly on it's sensitivity, accuracy and low fluid consumption. The CSF samples were normal with regard to pressure, color, citology and it's content in glucose, chloride and urea. Complement fixation tests for syphilis and cisticercosis, as well as the globulin tests (Pandy, Nonne — Appelt and Takata-Ara) were negative. The average value and the normal range of cisternal fluid total protein was calculated for the mixed population (males and females). The mean protein value was 26.78 mg/100 ml, the lower and upper limits were respectively 13.20 and 40.36 mg/100 ml. These data are higher than those stated in some publications, and factors that could interfere in the different results mentioned in the literature are briefly discussed. Since the statistical analysis of the results showed that the mean total protein concentration in males (28.76 mg/100 ml) was higher than in females (23.91 mg/100 ml), normal limits for each sex were established: 16.96 to 47.13 mg/100 ml for males and 14.76 to 42.76 mg/100 ml for females.

Proteínas totais do liquido cefalorraqueano obtido por punção da cisterna magna: valores normais. Variações ligadas ao sexo

Total protein content of normal cisternal cerebrospinal fluid: Variations related to sex

L. M. Singer Vermes^I; Rubens Guimarães Ferri^II; J. Aisen^III; J. M. Marlet^IV

^IProf. Assistente do Departamento de Microbiologia e Imunologia do Instituto de Ciências Biomédicas da Univ. de São Paulo

^IIProf. Livre-Docente do Departamento de Microbiologia e Imunologia do Instituto de Ciências Biomédicas da Univ. de São Paulo

^IIIAssistente da Clínica Neurológica da Faculdade de Medicina da Universidade de São Paulo

^IVProf. Livre-Docente do Departamento de Medicina Preventiva da Faculdade de Medicina da Univ. de São Paulo

RESUMO

Aplicando o método de Lowry, determinamos as proteínas totais do LCR considerado normal de 98 pessoas: 58 do sexo masculino e 40 do feminino. Todas as amostras de LCR foram obtidas por punção da cisterna magna (LCR-SO).

Mediante tratamento estatístico dos valores obtidos, estabelecemos as taxas normais de proteinorraquia cisternal para a população mista: limite superior de 40,36 mg/100 ml, valor médio de 26,78 mg/100 e limite inferior de 13,20 mg/100 ml. Estes valores são superiores àqueles referidos na literatura.

Foi verificado que a proteinorraquia total é, em média, significantemente maior nos indivíduos do sexo masculino, que nos de feminino. Consequentemente estabelecemos em separado os níveis normais de proteínas totais do LCR-SO para pessoas do sexo masculino e feminino.

As taxas médias de proteínas totais do LCR-SO de pessoas do sexo masculino e feminino são de 28,76 e 23,91 mg/100 ml, respectivamente; os limites normais vão de 16,96 a 47,13 mg/100 ml para o sexo masculino e de 14,76 a 42,76 mg/100 ml para o sexo feminino.

SUMMARY

The total protein content of CSF collected from the cisterna magna (cisternal fluid) of 98 patients (58 males and 40 females) with no neurological diseases was determined by the colorimetric method devised by Lowry and co-workers. This method has been previously chosen based mainly on it's sensitivity, accuracy and low fluid consumption. The CSF samples were normal with regard to pressure, color, citology and it's content in glucose, chloride and urea. Complement fixation tests for syphilis and cisticercosis, as well as the globulin tests (Pandy, Nonne Appelt and Takata-Ara) were negative.

The average value and the normal range of cisternal fluid total protein was calculated for the mixed population (males and females). The mean protein value was 26.78 mg/100 ml, the lower and upper limits were respectively 13.20 and 40.36 mg/100 ml. These data are higher than those stated in some publications, and factors that could interfere in the different results mentioned in the literature are briefly discussed.

Since the statistical analysis of the results showed that the mean total protein concentration in males (28.76 mg/100 ml) was higher than in females (23.91 mg/100 ml), normal limits for each sex were established: 16.96 to 47.13 mg/100 ml for males and 14.76 to 42.76 mg/100 ml for females.

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9. IZIKOWITZ, S. apud FLODEN, C.H.⁵

Departamento de Microbiologia e Imunologia Instituto de Ciências Biomédicas Universidade de São Paulo Caixa Postal 2921 01000 São Paulo, SP Brasil.

1. BOSSAK, H.N.; ROSENBERG, A.A. & HARRIS, A. A quantitative turbidimetric method for the determination of spinal fluid protein. J. vener. Dis. Inform. 30:100, 1949.
2. CASTAIGNE, P.; LHERMITTE, F.; SCHULLER, E.; ROUQUES, C. & LORIDAN, M. Étude électrophorétique des proteines du liquide céphalo-rachidien au cours de la sclérose en plaques. Rev. neurol. (Paris) 124:97, 1971.
3. DENCKER, S.J. Variation of total cerebrospinal fluid proteins and cells with sex and age. Wld. Neurol. 3:778, 1962.
4. DENCKER, S.J. & ZETHRAEUS, S. Sex differences in total protein content of cerebrospinal fluid. Acta psychiat. scand. 36:76, 1961.
5. FLODEN, C.H. Cerebrospinal fluid protein in syphilis. Acta derm.-Venereol. (Stockholm) 35 (Suppl. 32) :5-84, 1955.
6. GILLAND, O. Lumbar cerebrospinal fluid total proteins in healthy subjects. Acta neurol. scand. 43:526, 1967.
7. HEER, E.E. & MARGNI, R.A. Electro e Immunoelectroforesis. Manual de Laboratório e Interpretaciones Fundamentales. Gumersindo F. Fernandez, Buenos Aires, 1971, p. 269-276.
8. HILL, N.C.; McKENZIE, B.F.; McGUCKIN, W.F.; GOLDSTEIN, N.P. & SVIEN, H.J. Proteins, glycoproteins and lipoproteins in the serum and cerebrospinal fluid of healthy subjects. Proc. Staff. meet. Mayo Clin. 33:686, 1958.
10. JUNG, P.R.; RIEDER, H.P. & JELTSCH, C. Normalwerte des Totalproteins im Lumbal liquor: Alters-, Geschlechts- und Umweltabhängigkeit. Klin. Wschr. 51:810, 1973.
11. LOWRY, O.H.; ROSEBROUGH, N.J.; FARR, A.L. & RANDALL, R.J. Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265, 1951.
12. MADONICK, M. & WEISSMAN, F. The total spinal fluid protein concentration in patients over 65. Geriatrics 10:533, 1955.
13. MARRON, T.U. Errors in spinal fluid protein estimation with sulphosalicylic acid reagent. Amer. J. clin. Path. 12:37, 1942.
14. MERTTN, J.; WISSER, H. & ROERR, P. Untersuchung über den Normalbereich des Gesamteiweisses und der Eiweissfraktionen des Liquor cerebrospinalis nach elektrophoretischer Trennung auf Celluloseacetatfolie. Z. klin. Chem. 9: 337, 1971.
15. MEULEMANS, O. Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid. Clin. chim. Acta 5:757, 1960.
16. MULLER, O.H.; JAWORSKI, A.A.; SILVERMAN, A.C. & ELWOOD, M.J. The effect of age on the protein concentration of cerebrospinal fluid of "normal" individuals and patients with poliomyelitis and other diseases. Amer. J. med. Sci. 228:510, 1954.
17. PRICE, J.D.; GANT, P.W. & SAUL, K.J. Normal concentration of lactate, glucose and protein in cerebrospinal fluid, and the diagnostic implications of abnormal concentrations. Clin. Chem. 16:562, 1970.
18. RIEDER, H.P. Eine neue Modifikation der Cu-Folin Methode zur Bestimmung des Total-proteins im Liquor cerebrospinalis. Klin. Wschr. 44:1036, 1966.
19. RIEDER, H.P. & WÜTHRICH, R. Die Cu-Folin Methode zur Bestimmung des Gesamteiweissgehaltes im liquor. Klin. Wschr. 40:1070, 1962.
20. SPINA-FRANÇA, A. & AMAR, I. Valores normais da concentração do líquido cefalorraquidiano: variações ligadas ao local de colheita da amostra. Arq. Neuro-Psiquiat. (São Paulo) 19:220, 1961.
21. SPINA-FRANÇA, A. & AMAR, T. Proteinorraquia total. Variações relacionadas ao sexo e à idade. Arq. Neuro-Psiquiat. (São Paulo) 21:13, 1963.
22. VERMES, L.M.S. Proteínas totais do líquido cefalorraqueano: métodos de determinação e níveis normais (variações ligadas ao sexo, idade e local de punção). São Paulo, 1975 (Dissertação de mestrado Inst. Ciências Biom. Univers. São Paulo).
23. WIDELL, S. On the cerebrospinal fluid in normal children and in patients with acute abacterial meningo-encephalitis. Acta paediat. (Uppsala) 47(Suppl. 115):5, 1958.

Datas de Publicação

Publicação nesta coleção
25 Fev 2013
Data do Fascículo
Dez 1976

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BOSSAK, H.N.; ROSENBERG, A.A. & HARRIS, A. A quantitative turbidimetric method for the determination of spinal fluid protein. J. vener. Dis. Inform. 30:100, 1949.

[2] 2. CASTAIGNE, P.; LHERMITTE, F.; SCHULLER, E.; ROUQUES, C. & LORIDAN, M. Étude électrophorétique des proteines du liquide céphalo-rachidien au cours de la sclérose en plaques. Rev. neurol. (Paris) 124:97, 1971.

[3] 3. DENCKER, S.J. Variation of total cerebrospinal fluid proteins and cells with sex and age. Wld. Neurol. 3:778, 1962.

[4] 4. DENCKER, S.J. & ZETHRAEUS, S. Sex differences in total protein content of cerebrospinal fluid. Acta psychiat. scand. 36:76, 1961.

[5] 5. FLODEN, C.H. Cerebrospinal fluid protein in syphilis. Acta derm.-Venereol. (Stockholm) 35 (Suppl. 32) :5-84, 1955.

[6] 6. GILLAND, O. Lumbar cerebrospinal fluid total proteins in healthy subjects. Acta neurol. scand. 43:526, 1967.

[7] 7. HEER, E.E. & MARGNI, R.A. Electro e Immunoelectroforesis. Manual de Laboratório e Interpretaciones Fundamentales. Gumersindo F. Fernandez, Buenos Aires, 1971, p. 269-276.

[8] 8. HILL, N.C.; McKENZIE, B.F.; McGUCKIN, W.F.; GOLDSTEIN, N.P. & SVIEN, H.J. Proteins, glycoproteins and lipoproteins in the serum and cerebrospinal fluid of healthy subjects. Proc. Staff. meet. Mayo Clin. 33:686, 1958.

[9] 10. JUNG, P.R.; RIEDER, H.P. & JELTSCH, C. Normalwerte des Totalproteins im Lumbal liquor: Alters-, Geschlechts- und Umweltabhängigkeit. Klin. Wschr. 51:810, 1973.

[10] 11. LOWRY, O.H.; ROSEBROUGH, N.J.; FARR, A.L. & RANDALL, R.J. Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265, 1951.

[11] 12. MADONICK, M. & WEISSMAN, F. The total spinal fluid protein concentration in patients over 65. Geriatrics 10:533, 1955.

[12] 13. MARRON, T.U. Errors in spinal fluid protein estimation with sulphosalicylic acid reagent. Amer. J. clin. Path. 12:37, 1942.

[13] 14. MERTTN, J.; WISSER, H. & ROERR, P. Untersuchung über den Normalbereich des Gesamteiweisses und der Eiweissfraktionen des Liquor cerebrospinalis nach elektrophoretischer Trennung auf Celluloseacetatfolie. Z. klin. Chem. 9: 337, 1971.

[14] 15. MEULEMANS, O. Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid. Clin. chim. Acta 5:757, 1960.

[15] 16. MULLER, O.H.; JAWORSKI, A.A.; SILVERMAN, A.C. & ELWOOD, M.J. The effect of age on the protein concentration of cerebrospinal fluid of "normal" individuals and patients with poliomyelitis and other diseases. Amer. J. med. Sci. 228:510, 1954.

[16] 17. PRICE, J.D.; GANT, P.W. & SAUL, K.J. Normal concentration of lactate, glucose and protein in cerebrospinal fluid, and the diagnostic implications of abnormal concentrations. Clin. Chem. 16:562, 1970.

[17] 18. RIEDER, H.P. Eine neue Modifikation der Cu-Folin Methode zur Bestimmung des Total-proteins im Liquor cerebrospinalis. Klin. Wschr. 44:1036, 1966.

[18] 19. RIEDER, H.P. & WÜTHRICH, R. Die Cu-Folin Methode zur Bestimmung des Gesamteiweissgehaltes im liquor. Klin. Wschr. 40:1070, 1962.

[19] 20. SPINA-FRANÇA, A. & AMAR, I. Valores normais da concentração do líquido cefalorraquidiano: variações ligadas ao local de colheita da amostra. Arq. Neuro-Psiquiat. (São Paulo) 19:220, 1961.

[20] 21. SPINA-FRANÇA, A. & AMAR, T. Proteinorraquia total. Variações relacionadas ao sexo e à idade. Arq. Neuro-Psiquiat. (São Paulo) 21:13, 1963.

[21] 22. VERMES, L.M.S. Proteínas totais do líquido cefalorraqueano: métodos de determinação e níveis normais (variações ligadas ao sexo, idade e local de punção). São Paulo, 1975 (Dissertação de mestrado Inst. Ciências Biom. Univers. São Paulo).

[22] 23. WIDELL, S. On the cerebrospinal fluid in normal children and in patients with acute abacterial meningo-encephalitis. Acta paediat. (Uppsala) 47(Suppl. 115):5, 1958.