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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Arq. Neuro-Psiquiatr. vol.41 no.1 São Paulo Mar. 1983

http://dx.doi.org/10.1590/S0004-282X1983000100001 

Proteinas do liquido cefalorraqueano: I. Estudo comparativo entre metodos de concentração

 

Cerebrospinal fluid proteins: I. Comparison of protein concentration methods

 

 

Lucia M. Singer Vermes

Professora Assistente do Departamento de Microbiologia e Imunologia do Instituto de Ciências Biomédicas da Universidade de São Paulo

 

 


RESUMO

No intuito de escolher entre métodos de concentração, aplicáveis ao LCR, aquele que, não provocando desnaturação de proteínas, seja o mais exato, preciso e de fácil aplicação em laboratórios de rotina, foram estudados quatro procedimentos: diálise contra solução de goma-arábica, centrífugo-ultrafiltração, absorção de substâncias de baixo peso molecular por gel seco de poliacrilamida e ultrafiltração sob pressão positiva. Concluiu-se que a diálise contra solução de goma-arábica, por ser preciso, relativamente exato, simples e pouco oneroso, é o mais satisfatório dentre os métodos estudados.


SUMMARY

Four protein concentration methods, applicable to cerebrospinal fluid, were compared in order to choose a procedure that not causing protein denaturation, is accurate, precise and easy to perform in routine laboratories. The studied methods were: dialysis against arabic-gum solution, centrifuge-ultrafiltration, absorption of low molecular weight substances by dry polyacrylamide gel and pressure ultrafiltration. Blood serum was diluted, then concentrated by these procedures and analyzed by cellulose acetate electrophoresis; the undiluted serum served as control.
It was observed that two methods: pressure ultrafiltration and absorption of low molecular weight substances by polyacrylamide hydrogel, are not suitable for concentrating diluted fluids because they frequently cause protein denaturation.
It was concluded that dialysis against arabic-gum is the most suitable, of the methods studied, for application in routine laboratories taking in account that this procedure is precise, relatively accurate, simple and of low cost.


 

 

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Resumo da tese de doutoramento "Líquido Cefalorraqueano Normal: métodos de concentração, proteinograma e imuneglobulinas", defendida no Departamento de Microbiologia e Imunologia do Instituto de Ciências Biomédicas da Universidade de São Paulo.
Departamento de Microbiologia e Imunologia - Instituto de Ciências Biomédicas - Universidade de São Paulo - Caixa Postal 2921 - 01000 - São Paulo, SP - Brasil.

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