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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Arq. Neuro-Psiquiatr. vol.41 no.3 São Paulo Sept. 1983

http://dx.doi.org/10.1590/S0004-282X1983000300002 

Morphometry study on pre- and post-hatching nerve cell bodies of lumbar spinal ganglia of Gallus domesticus

 

Estudo morfométrico sobre corpos de células nervosas de gânglios espinais lombares de Gallus domesticus antes e depois da eclosão

 

 

Claudio A. Ferraz de CarvalhoI; Ciro F. da SilvaII; Bonfin A. Silva JuniorIII; M. Vilma de AraujoIV

IFull Professor. Department of Anatomy, ICB, São Paulo University
IIAssistant Professor. Department of Anatomy, ICB, São Paulo University
IIIResident Senior. Department of Anatomy, ICB, São Paulo University
IVBiologist. Department of Anatomy, ICB, São Paulo University

 

 


SUMMARY

A cytomorphometric study was performed in lumbar spinal ganglia neurons of Gallus domesticus on the 10th and 18th incubation days and 8th, 35th, 61st, and 120th post-hatching days. The absolute volume of nucleus and relative volume of cytoplasm were respectively estimated by the Bach2 caryometric method and by point-counting volumetry, carried out in 0.5mm thick araldite sections. The relative volume, the surface-to-volume ratio and the total surface of RER, SER, mitochondria, dense bodies, Golgi complex and the relative volume of hyaloplasm inside and outside the Nissl bodies were estimated from electronmicrographs by the Weibel et al.58 method. The conclusions were: a) there was an increase of the cell volume and a decrease of the nucleo-cytoplasmic ratio, particularly between the first two ages; b) the relative volumes of RER and SER change inversely with respect to each other: the RER increases before hatching, decreasing progressively afterwards; the changes of relative volume of dense bodies are similar to those of the RER, and the mitochondria show relatively small variations concerning the same parameter; c) the relative volume of hyaloplasm inside the Nissl bodies decreases while those outside increases; d) the surface-to-volume ratio drops sharply for all organelles from the 10th to the 18th day of incubation; after hatching, a tendency to increase is observed; e) the membrane surface-to-cytoplasmic volume ratio decreases for all organelles from the 10th to the 18th day of incubation; after hatching, this ratio increases slightly for mitochondria and Golgi complex, sharply for SER, dropping for dense bodies. The RER values alternate regularly.


RESUMO

Foi realizado estudo citomorfométrico em neurônios de gânglios espinais lumbares de Gallus domesticus no 10º e 18º dias de incubação e no 8º, 35º, 61º e 120º dias após a eclosão. Os volumes absoluto do núcleo e relativo do citoplasma foram estimados, respectivamente, pelo método cariométrico de Bach2 e pela volumetria de contagem de pontos, em cortes de araldite de 0.5mm. O volume relativo, a relação superfície/volume e a superfície total do RER e SER, mitocôndria, corpos densos, complexo de Golgi e volume relativo de hialoplasma, no interior e fora dos corpos de Nissl, foram estimados a partir de eletron-micrografias, pelo método de Weibel et al.58. As conclusões foram: a) houve aumento do volume celular e diminuição da relação núcleo-citoplasmática, particularmente entre as duas primeiras idades; b) os volumes relativos do RER e SER variam inversamente, entre si: o RER aumenta antes da eclosão, decrescendo, após, progressivamente; as variações do volume relativo dos corpos densos são semelhantes àquelas do RER e as mitocôndrias mostram relativamente, pequenas variações, no que concerne ao mesmo parâmetro; c) o volume relativo do hialoplasma no interior dos corpos de Nissl diminui, enquanto o situado no interior, aumenta; d) a relação superfície/volume decresce muito para todas as organelas, do 10º para o 18º dia de incubação; após a eclosão foi observada tendência à elevação; e) a relação superfície de membrana volume citoplasmático cai para todas as organelas, do 10º para o 18º dia de incubação; após a eclosão, esta relação diminui levemente para mitocôndrias e complexo de Golgi, fortemente para o SER, caindo, também, para corpos densos. Os valores do RER alternam-se, regularmente.


 

 

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This study was carried out with aid of grants from the CNPq (Brasil). (Processo 2222.1694/78).
Departamento de Anatomia - ICB - Bloco 30 - Universidade de São Paulo - Caixa Postal 4365 - 01000 São Paulo, SP - Brasil.

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