Cholinesterase activity of lamellated sensory corpuscles in the rat lip

Watanabe, II-Sei; Ide, Chizuka

doi:10.1590/S0004-282X1986000400002

Abstracts

Non-specific cholinesterase (ChE) activity was demonstrated in lamellated sensory corpuscles of the rat lip by light and electron microscopy using Karnovsky and Root's method. ChE activity was present in the interlamellar spaces between neighbouring lamellae as well as in the periaxonal space between axon terminals and their adjacent lamellae. Reaction products of ChE activity were also deposited in some caveolae of the lamellar cell plasma membrane, and in the cisternae of the rough endoplasmic reticulum as well as in the nuclear envelope of lamellar cell bodies. No definite reaction products were detected within the axon terminals. These findings show that the lamellated corpuscles in the rat lip, like other mechanoreceptors, have an intense ChE activity which is mainly associated with lamellar cells. It can be said that ChE histochemistry is useful to detect mechanoreceptors. The functional significance of ChE in mechanoreceptors is discussed.

A atividade de colinesterase inespecífica foi demonstrada em corpúsculos lamelares sensitivos do lábio de ratos através da microscopia óptica e eletrônica de transmissão, utilizando o método de Karnovsky & Roots. A atividade colinesterásica estava presente no espaço interlamelar entre as lamelas sucessivas, bem como no espaço entre o axônio terminal e a lamela adjacente. Produtos de reação da atividade colinesterásica eram bem evidentes em algumas cavéolas da membrana plasmática da célula lamelar e em cisternas do retículo endoplasmático granular, bem como na membrana nuclear. Produtos inespecíficos de reação foram detectados dentro do axônio terminal. Esses dados mostram que os corpúsculos lamelares do lábio de ratos, como outros mecanorreceptores, apresentam intensa atividade colinesterásica, a qual é associada principalmente com as células lamelares. Pode-se dizer que a histoquímica de colinesterase é útil para detectar os mecanorreceptores. A significância funcional da colinesterase em mecanorreceptores é discutida.

Cholinesterase activity of lamellated sensory corpuscles in the rat lip

Atividade de colinesterase em corpúsculos lamelares sensitivos no lábio de ratos

II-Sei Watanabe^I; Chizuka Ide^II

^IProfessor Titular de Anatomia do Departamento de Anatomia, Instituto de Ciências Biomédicas da Universidade de São Paulo

^IIProfessor Titular de Anatomia do Departamento de Anatomia, "Iwate Medicai University School of Medicine, Morioka, Japan»

SUMMARY

Non-specific cholinesterase (ChE) activity was demonstrated in lamellated sensory corpuscles of the rat lip by light and electron microscopy using Karnovsky and Root's method. ChE activity was present in the interlamellar spaces between neighbouring lamellae as well as in the periaxonal space between axon terminals and their adjacent lamellae. Reaction products of ChE activity were also deposited in some caveolae of the lamellar cell plasma membrane, and in the cisternae of the rough endoplasmic reticulum as well as in the nuclear envelope of lamellar cell bodies. No definite reaction products were detected within the axon terminals. These findings show that the lamellated corpuscles in the rat lip, like other mechanoreceptors, have an intense ChE activity which is mainly associated with lamellar cells. It can be said that ChE histochemistry is useful to detect mechanoreceptors. The functional significance of ChE in mechanoreceptors is discussed.

RESUMO

A atividade de colinesterase inespecífica foi demonstrada em corpúsculos lamelares sensitivos do lábio de ratos através da microscopia óptica e eletrônica de transmissão, utilizando o método de Karnovsky & Roots. A atividade colinesterásica estava presente no espaço interlamelar entre as lamelas sucessivas, bem como no espaço entre o axônio terminal e a lamela adjacente. Produtos de reação da atividade colinesterásica eram bem evidentes em algumas cavéolas da membrana plasmática da célula lamelar e em cisternas do retículo endoplasmático granular, bem como na membrana nuclear. Produtos inespecíficos de reação foram detectados dentro do axônio terminal. Esses dados mostram que os corpúsculos lamelares do lábio de ratos, como outros mecanorreceptores, apresentam intensa atividade colinesterásica, a qual é associada principalmente com as células lamelares. Pode-se dizer que a histoquímica de colinesterase é útil para detectar os mecanorreceptores. A significância funcional da colinesterase em mecanorreceptores é discutida.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Acknowledgements - This study was supported by Japan International Cooperation Agency (JICA, Grant Nº 8205491).

Departamento de Anatomia, Instituto de Ciências Biomédicas - Universidade de S. Paulo - Caixa Postal 4365 - 01000 - São Paulo. SP - Brasil.

1. ANDRES, K.H. - Uber die Feinstruktur der Rezeptoren an Sinushaaren. Z. Zellforsch. 75:339, 1966.
2. CAUNA, N. - The distribution of Cholinesterase in the cutaneous receptor organs, specially touch corpuscles of the human finger. J. Histochem. Cytochem. 8:367, 1960.
3. CHOUCHKOV, Ch. - Cutaneous receptors. Adv. Anat. Embryol. Cell Biol. 54:5, 1978.
4. DUBOVY, P. - The problem of the localization of Cholinesterase activity in Pacinian corpuscles by electron microscopy. Histochem. J. 15:287, 1983.
5. HALATA, Z. & MUNGER, B.L. - The sensory innervation of primate facial skin. II. Vermilion border and mucosa of lip. Brain Res. Rev. 5:81, 1983.
6. HEBB, C. & HILL, K.J. - PseudoCholinesterase in Pacinian corpuscles. Nature 175:597, 1955.
7. HURLEY, H.J. - Non-specific Cholinesterase in specialized sensory nerve endings of human genital skin. Brit. J. Derm. 70:284, 1958.
8. HURLEY, H.J., Jr. & MESCON, H. - Localization of non-specific Cholinesterase in Meissner's corpuscle in human skin. Brit. J. Derm. 68:290, 1956.
9. IDE, C. - Degeneration of mouse digital corpuscles. Amer. J. Anat. 163:59, 1982.
10. IDE, C. & SAITO, T. - Electron microscopic histochemistry of Cholinesterase activity of Vater-Pacini corpuscle. Acta histochem. cytochem. 13:298, 1980.
11. IDE, C. & SAITO, T. - Electron microscopic cytochemistry of cholinesterase activity of mouse digital corpuscle. Acta histochem. cytochem. 13:218, 1980.
12. IGGO, A. - Cutaneous receptors. In J.I. Hubbard (ed.): The Peripheral Nervous System. Plenum Press, New York, 1974, pg. 347.
13. ISHIBASHI, Y.; NIIMURA, M. & KAWAMURA, T. - Ultrastructural localization of cholinesterase activity in human Haarscheiben. Acta histocfiem. cytochem. 4:191, 1971.
14. JANIG, W. - Morphology of rapidly and slowly adapting mechanoreceptors in the hairless skin of the cat's hindfoot. Brain Res. 28:217, 1971.
15. KARNOVSXY, M.J. & ROOTS, L. - A <> thiocholine method for cholinesterase. J. Histochem. Cytochem. 12:219, 1964.
16. KRAUSE, W. - On the termination of nerves in the conjuctiva. J. Anat. 1:346, 1867.
17. MARTINEZ, I.R., Jr. & PEKARTHY, J.M. - Ultrastructure of encapsulated nerve endings in rat gingiva. Amer. J. Anat. 140:135, 1974.
18. MUNG-ER, B.D. - Cytology and ultrastructure of sensory receptors in the adult and newborn or mate tongue (development in the fetus and infant). In J.F. Bosma (ed.): Fourth Symposium on Oral Sensation and Perception, DHEW Publication No. (NIH) 73-546, 1973, pg. 75.
19. MUNGER, B.L.. - Cytology of mechanoreceptors in oral mucosa and facial skin of the rhesus monkey. In D.B. Tower & R.O. Brady (eds.): The Nervous System. The Basic Neuroscience. Raven Press, New York, 1975, vol. 1, pg. 71.
20. MUNGER, B.L.. & PUBOLS, L..M. - The sensorineural organization of the digital skin of the racoon. Brain Behav. Evol. 5:367, 1972.
21. SANES, J.R.; MARCHALL, L.M. & McMAHAN, U.J. - Reinnervation of muscle fiber basa_ lamina after removal of myofibers. J. Cell Biol. 78:176, 1978.
22. SAXOD, R. - Activité cholinestérasique des corpuscles sensoriels cutanés de Herbst et de Grandry. Histochemie 34:43, 1973.
23. SETO, H. - Studies on the Sensory Innervation - Human Sensibility. Igaku Shoin, Tokyo, 1963.
24. SETO, H. - Color Atlas on Sensory Innervation. Published by Department of Anatomy, Tohoku University School of Medicine. Sendai, Japan, 1977, pg. 20.
25. SPASSOVA, I. - Ultrastuctural relationships between the receptor nerve fiber and surrounding lamellae in Krause end-bulbs. Acta anat. 109:360, 1981.
26. TOYOMA, Y. - The morphology of sensory corpuscles in joint capsule: ultrastructural and histochemical ^rtudy. J. orthop. Assoc. 59:1985, in press.
27. WATANABE, I. & YAMAJ A, E. - The fine structure of lamellated nerve endings found in the rat gingiva. Arch. Hist. jpn. 46:173, 1983.
28. WINKELMANN, R.K. - Nerve Endings in Normal and Pathologic Skin. Charles C. Thomas, Springfield, 1960, pg. 81.
29. YOKOTA, R. ; IDE, C. : NITATORI, T. & ONODERA, S. - Cholinesterase activity in the carotid sinus baroreceptor. Acta histochem. cytochem. 15:537, 1982.
30. YOKOTA, R. ; IDE, C. ; NITATORI, T. & ONODERA, S. - Electron microscopy histochemistry of cholinesterase activity in the baroreceptor of the cat atrial endocardium. Acta histochem. cytochem. 16:129, 1983.

Publication Dates

Publication in this collection
29 June 2011
Date of issue
Dec 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ANDRES, K.H. - Uber die Feinstruktur der Rezeptoren an Sinushaaren. Z. Zellforsch. 75:339, 1966.

[2] 2. CAUNA, N. - The distribution of Cholinesterase in the cutaneous receptor organs, specially touch corpuscles of the human finger. J. Histochem. Cytochem. 8:367, 1960.

[3] 3. CHOUCHKOV, Ch. - Cutaneous receptors. Adv. Anat. Embryol. Cell Biol. 54:5, 1978.

[4] 4. DUBOVY, P. - The problem of the localization of Cholinesterase activity in Pacinian corpuscles by electron microscopy. Histochem. J. 15:287, 1983.

[5] 5. HALATA, Z. & MUNGER, B.L. - The sensory innervation of primate facial skin. II. Vermilion border and mucosa of lip. Brain Res. Rev. 5:81, 1983.

[6] 6. HEBB, C. & HILL, K.J. - PseudoCholinesterase in Pacinian corpuscles. Nature 175:597, 1955.

[7] 7. HURLEY, H.J. - Non-specific Cholinesterase in specialized sensory nerve endings of human genital skin. Brit. J. Derm. 70:284, 1958.

[8] 8. HURLEY, H.J., Jr. & MESCON, H. - Localization of non-specific Cholinesterase in Meissner's corpuscle in human skin. Brit. J. Derm. 68:290, 1956.

[9] 9. IDE, C. - Degeneration of mouse digital corpuscles. Amer. J. Anat. 163:59, 1982.

[10] 10. IDE, C. & SAITO, T. - Electron microscopic histochemistry of Cholinesterase activity of Vater-Pacini corpuscle. Acta histochem. cytochem. 13:298, 1980.

[11] 11. IDE, C. & SAITO, T. - Electron microscopic cytochemistry of cholinesterase activity of mouse digital corpuscle. Acta histochem. cytochem. 13:218, 1980.

[12] 12. IGGO, A. - Cutaneous receptors. In J.I. Hubbard (ed.): The Peripheral Nervous System. Plenum Press, New York, 1974, pg. 347.

[13] 13. ISHIBASHI, Y.; NIIMURA, M. & KAWAMURA, T. - Ultrastructural localization of cholinesterase activity in human Haarscheiben. Acta histocfiem. cytochem. 4:191, 1971.

[14] 14. JANIG, W. - Morphology of rapidly and slowly adapting mechanoreceptors in the hairless skin of the cat's hindfoot. Brain Res. 28:217, 1971.

[15] 15. KARNOVSXY, M.J. & ROOTS, L. - A <> thiocholine method for cholinesterase. J. Histochem. Cytochem. 12:219, 1964.

[16] 16. KRAUSE, W. - On the termination of nerves in the conjuctiva. J. Anat. 1:346, 1867.

[17] 17. MARTINEZ, I.R., Jr. & PEKARTHY, J.M. - Ultrastructure of encapsulated nerve endings in rat gingiva. Amer. J. Anat. 140:135, 1974.

[18] 18. MUNG-ER, B.D. - Cytology and ultrastructure of sensory receptors in the adult and newborn or mate tongue (development in the fetus and infant). In J.F. Bosma (ed.): Fourth Symposium on Oral Sensation and Perception, DHEW Publication No. (NIH) 73-546, 1973, pg. 75.

[19] 19. MUNGER, B.L.. - Cytology of mechanoreceptors in oral mucosa and facial skin of the rhesus monkey. In D.B. Tower & R.O. Brady (eds.): The Nervous System. The Basic Neuroscience. Raven Press, New York, 1975, vol. 1, pg. 71.

[20] 20. MUNGER, B.L.. & PUBOLS, L..M. - The sensorineural organization of the digital skin of the racoon. Brain Behav. Evol. 5:367, 1972.

[21] 21. SANES, J.R.; MARCHALL, L.M. & McMAHAN, U.J. - Reinnervation of muscle fiber basa_ lamina after removal of myofibers. J. Cell Biol. 78:176, 1978.

[22] 22. SAXOD, R. - Activité cholinestérasique des corpuscles sensoriels cutanés de Herbst et de Grandry. Histochemie 34:43, 1973.

[23] 23. SETO, H. - Studies on the Sensory Innervation - Human Sensibility. Igaku Shoin, Tokyo, 1963.

[24] 24. SETO, H. - Color Atlas on Sensory Innervation. Published by Department of Anatomy, Tohoku University School of Medicine. Sendai, Japan, 1977, pg. 20.

[25] 25. SPASSOVA, I. - Ultrastuctural relationships between the receptor nerve fiber and surrounding lamellae in Krause end-bulbs. Acta anat. 109:360, 1981.

[26] 26. TOYOMA, Y. - The morphology of sensory corpuscles in joint capsule: ultrastructural and histochemical ^rtudy. J. orthop. Assoc. 59:1985, in press.

[27] 27. WATANABE, I. & YAMAJ A, E. - The fine structure of lamellated nerve endings found in the rat gingiva. Arch. Hist. jpn. 46:173, 1983.

[28] 28. WINKELMANN, R.K. - Nerve Endings in Normal and Pathologic Skin. Charles C. Thomas, Springfield, 1960, pg. 81.

[29] 29. YOKOTA, R. ; IDE, C. : NITATORI, T. & ONODERA, S. - Cholinesterase activity in the carotid sinus baroreceptor. Acta histochem. cytochem. 15:537, 1982.

[30] 30. YOKOTA, R. ; IDE, C. ; NITATORI, T. & ONODERA, S. - Electron microscopy histochemistry of cholinesterase activity in the baroreceptor of the cat atrial endocardium. Acta histochem. cytochem. 16:129, 1983.