Estudos ultraestruturais de linfócitos, polimorfonucleares e fagócitos de pacientes com doença de Duchenne

Minguetti, Guilberto; Kato, Mara A. D. Pianovski; Hofmeister, Robin M.

doi:10.1590/S0004-282X1988000100007

Resumos

Os autores estudaram por microscopia eletrônica células mononucleares e granulócitos obtidos do sangue de 10 pacientes com distrofia muscular de Duchenne. Embora vários estudos tenham demonstrado alterações bioquímicas e imunológicas nessas células do sangue, não foram encontradas alterações ultraestruturais neste estudo.

The authors have carried out an electron microscopy study of mononuclear cells and granulocytes obtained from 10 patients with Duchenne muscular dystrophy. Although several studies have demonstrated biochemical and immunological defects in these blood cells, no ultrastructural abnormalities were found in the present work.

Guilberto Minguetti^I; Mara A. D. Pianovski Kato^II; Robin M. Hofmeister^III

^IProfessor Adjunto de Neurologia - Disciplina de Neurologia do Departamento de Clínica Médica e Centro de Microscopia Eletrônica da Universidade Federal do Paraná (UFPR)

^IIResponsável pelo Serviço de Hematopediatria do Hospital de Clínicas da UFPR

^IIIProfessor Adjunto, Centro de Microcospia Eletrônica - Disciplina de Neurologia do Departamento de Clínica Médica e Centro de Microscopia Eletrônica da Universidade Federal do Paraná (UFPR)

RESUMO

Os autores estudaram por microscopia eletrônica células mononucleares e granulócitos obtidos do sangue de 10 pacientes com distrofia muscular de Duchenne. Embora vários estudos tenham demonstrado alterações bioquímicas e imunológicas nessas células do sangue, não foram encontradas alterações ultraestruturais neste estudo.

SUMMARY

The authors have carried out an electron microscopy study of mononuclear cells and granulocytes obtained from 10 patients with Duchenne muscular dystrophy. Although several studies have demonstrated biochemical and immunological defects in these blood cells, no ultrastructural abnormalities were found in the present work.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Trabalho realizado na Disciplina de Neurologia do Departamento de Clínica Médica e Centro de Microscopia Eletrônica da Universidade Federal do Paraná (UFPR)

Rua Brigadeiro Franco 122 - 80410 Curitiba PR - Brasil.

1. Bessis M - Cellules du Sang Normal et Pathologique. Masson, Paris, 1972.
2. Böyum A - Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest (Suppl) 97: 77, 1968.
3. Butterfield DA, Chesnut DB, Appel SH, Roses AD - Spin label study of erythrocyte mebrane fluidity in myotonic and Duchenne muscular dystrophy and congenital myotonia. Nature 263: 159, 1976.
4. Carr I - The Machrophage: A Review of Ultrastructure and Function. Academic Press, London, 1973.
5. Cawley JC, Hayhoe FGJ - Ultrastructure of Haemic Cells: A Cytological Atlas of Normal and Leukaemic Blood and Bone Marrow. Saunders, London, 1973.
6. Headings VE, Anyaibe SIO, Akindele J, Onaga M - An erythrocyte membrane antigen associated with X-linked muscular dystrophy. Arch Neurol 40: 300, 1983.
7. Mair WGP, Tomé FMS - Atlas of the Ultrastructure of Diseased Human Muscle. Churchill-Livingstone, Edinburgh, 1972.
8. Mawatari S, Miranda A, Rowland LP - Adenyl cyclase abnormality in Duchenne muscular dystrophy: muscle cells in culture. Neurology 26:1021, 1976.
9. Mawatari S, Igisu H, Kuroiwa Y, Miyoshino S - Na ⁺ + K ⁺ ATPase of erythrocyte membranes in Duchenne muscular dystrophy. Neurology 31: 293, 1981.
10. Minguetti G, Mair WGP - Ultrastructure of developing human muscle. Biol Neonate 40: 276, 1981.
11. Mollman JE, Pleasure DE - Erythrocyte calcium transport in myotonic and fascioscapulo-humeral muscular dystrophy. Neurology 34: 1514, 1984.
12. Pickard NA, Gruener HD, Verril HD, Isaacs ER, Robinow M, Nance WE, Myers EC, Goldsmith B - Systemic membrane defect in the proximal muscular dystrophies. N Engl J Med 299: 841, 1978.
13. Plishker GA, Gitelman HJ, Appel SH - Myotonic muscular dystrophy: altered calcium transport in erythrocytes. Science 200: 323, 1978.
14. Roses AD, Herbstreith MH, Appel SH - Membrane protein kinase alteration in Duchenne muscular dystrophy. Nature 254: 350, 1975.
15. Rowland LP - Pathogenesis of muscular dystrophy. Arch Neurol 33:315, 1976.
16. Sarpel G, Lubansky HJ, Danon MJ, Omachi A - Erythrocytes in muscular dystrophy:investigation with 31P nuclear magnetic resonance spectroscopy. Arch Neurol 38: 271, 1981.
17. Walton JN - Muscular dystrophy: some recent developments in research. Isr J Med Sci 13 :152, 1977.
18. Yaron R, Meyer S, More, R, Liebergall M, Eldor A - Platelet abnormalities in muscular dystrophy. Thromb Haemosts (Stuttgart) 49: 168, 1983.
19. Yoshida M, Ando K, Satoyoshi E - Abnormalities of erythrocytes in Duchenne muscular dystrophy. Ann Neurol 13: 649, 1983.

Estudos ultraestruturais de linfócitos, polimorfonucleares e fagócitos de pacientes com doença de Duchenne

Ultrastructure of lymphocytes, polymorphonuclears and phagocytes of patients with muscular dystrophy

Datas de Publicação

Publicação nesta coleção
22 Jun 2011
Data do Fascículo
Mar 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Bessis M - Cellules du Sang Normal et Pathologique. Masson, Paris, 1972.

[2] 2. Böyum A - Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest (Suppl) 97: 77, 1968.

[3] 3. Butterfield DA, Chesnut DB, Appel SH, Roses AD - Spin label study of erythrocyte mebrane fluidity in myotonic and Duchenne muscular dystrophy and congenital myotonia. Nature 263: 159, 1976.

[4] 4. Carr I - The Machrophage: A Review of Ultrastructure and Function. Academic Press, London, 1973.

[5] 5. Cawley JC, Hayhoe FGJ - Ultrastructure of Haemic Cells: A Cytological Atlas of Normal and Leukaemic Blood and Bone Marrow. Saunders, London, 1973.

[6] 6. Headings VE, Anyaibe SIO, Akindele J, Onaga M - An erythrocyte membrane antigen associated with X-linked muscular dystrophy. Arch Neurol 40: 300, 1983.

[7] 7. Mair WGP, Tomé FMS - Atlas of the Ultrastructure of Diseased Human Muscle. Churchill-Livingstone, Edinburgh, 1972.

[8] 8. Mawatari S, Miranda A, Rowland LP - Adenyl cyclase abnormality in Duchenne muscular dystrophy: muscle cells in culture. Neurology 26:1021, 1976.

[9] 9. Mawatari S, Igisu H, Kuroiwa Y, Miyoshino S - Na ⁺ + K ⁺ ATPase of erythrocyte membranes in Duchenne muscular dystrophy. Neurology 31: 293, 1981.

[10] 10. Minguetti G, Mair WGP - Ultrastructure of developing human muscle. Biol Neonate 40: 276, 1981.

[11] 11. Mollman JE, Pleasure DE - Erythrocyte calcium transport in myotonic and fascioscapulo-humeral muscular dystrophy. Neurology 34: 1514, 1984.

[12] 12. Pickard NA, Gruener HD, Verril HD, Isaacs ER, Robinow M, Nance WE, Myers EC, Goldsmith B - Systemic membrane defect in the proximal muscular dystrophies. N Engl J Med 299: 841, 1978.

[13] 13. Plishker GA, Gitelman HJ, Appel SH - Myotonic muscular dystrophy: altered calcium transport in erythrocytes. Science 200: 323, 1978.

[14] 14. Roses AD, Herbstreith MH, Appel SH - Membrane protein kinase alteration in Duchenne muscular dystrophy. Nature 254: 350, 1975.

[15] 15. Rowland LP - Pathogenesis of muscular dystrophy. Arch Neurol 33:315, 1976.

[16] 16. Sarpel G, Lubansky HJ, Danon MJ, Omachi A - Erythrocytes in muscular dystrophy:investigation with 31P nuclear magnetic resonance spectroscopy. Arch Neurol 38: 271, 1981.

[17] 17. Walton JN - Muscular dystrophy: some recent developments in research. Isr J Med Sci 13 :152, 1977.

[18] 18. Yaron R, Meyer S, More, R, Liebergall M, Eldor A - Platelet abnormalities in muscular dystrophy. Thromb Haemosts (Stuttgart) 49: 168, 1983.

[19] 19. Yoshida M, Ando K, Satoyoshi E - Abnormalities of erythrocytes in Duchenne muscular dystrophy. Ann Neurol 13: 649, 1983.