Enzyme linked immunosorbent assay (ELISA) for detection of IgG, IgM, IgE and IgA against cysticercus cellulosae in cerebrospinal fluid in neurocysticercosis (abstract)

Odashima, Newton Satoru

doi:10.1590/S0004-282X1997000300027

THESES

Enzyme linked immunosorbent assay (ELISA) for detection of IgG, IgM, IgE and IgA against cysticercus cellulosae in cerebrospinal fluid in neurocysticercosis (abstract)^* * Dissertação de Mestrado, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (Área: Neurologia). Orientador: Osvaldo Massaiti Takayanagui. ** Address: Laboratório de LCR, Departamento de Neurologia, Faculdade de Medicina de Ribeirão Preto, Campus Universitário USP, 14048-900 Ribeirão Preto SP. Brasil.

Reações imunoenzimáticas para detecção de IgG, IgM, IgE e IgA anticisticerco no líquido cefalorraquiano na neurocisticercose (Resumo)

Newton Satoru Odashima^** * Dissertação de Mestrado, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (Área: Neurologia). Orientador: Osvaldo Massaiti Takayanagui. ** Address: Laboratório de LCR, Departamento de Neurologia, Faculdade de Medicina de Ribeirão Preto, Campus Universitário USP, 14048-900 Ribeirão Preto SP. Brasil.

Enzyme linked immunosorbent assay (elisa) for detection of igg, igm, ige and iga against cysticercus cellulosae in cerebrospinal fluid in neurocysticercosis (abstract). Dissertation. Ribeirão Preto, 1997.

Neurocysticercosis (NCC) is a severe problem of public health in the developing countries acquiring special concern in our region where it is an endemic disease. It causes several disturbances in humans, resulting in elevated morbity and mortality, clinically manifesting in a proteiform way. The criterious use of subsidiary methods become imperative since these symptomatological multiplicity, standing out in this context the neuroimaging and immunological methods.

The present study had as objective the analysis of different classes of immunoglobulins, IgG, IgM, IgE and IgA, specific against cysticerci, in the cerebrospinal fluid (CSF), through the immunoenzymatic assay (ELISA), searching for correlations between these findings and clinical and tomographic elements.

We selected 85 CSF samples from patients, 26 of them with the active form of NCC, 17 with inactive form of NCC, and 42 with other neurological diseases. The selection was based on clinical, tomographic and CSF basis.

It was observed that the inactive form of NCC presents an immunological profile similar to that of the group without NCC. The active form presents elevation of all specific immunoglobulins studied being that pattern heterogeneous in each class, being highest the participation of IgG, IgM, IgE and IgA in decrescent order.

The highest values of mean optical density was observed in the cases with intraventricular cysts or signs of inflammation in the CSF and in the cases with multiple clinical manifestations.

The diagnostic potential of each immunoenzymatic test was assayed. The ELISA IgG showed the highest value, with sensibility of 88.5% and specificity of 93.2%.

The present stydy confirmed the importance of ELISA in the immunological diagnosis of NCC, particularly in the active forms.

Key words: neurocysticercosis, cerebrospinal fluid, immunological diagnosis, ELISA, cysticerci specific immunoglobulins (IgG, IgM, IgE, IgA).

*

Dissertação de Mestrado, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (Área: Neurologia). Orientador: Osvaldo Massaiti Takayanagui.

**

Address: Laboratório de LCR, Departamento de Neurologia, Faculdade de Medicina de Ribeirão Preto, Campus Universitário USP, 14048-900 Ribeirão Preto SP. Brasil.

Publication Dates

Publication in this collection
18 Oct 2010
Date of issue
Sept 1997

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