Morphological and quantitative analysis of the neurons of the myenteric plexus of the cecum of streptozotocin-induced diabetic rats

Zanoni, Jacqueline Nelisis; Miranda-Neto, Marcilio Hubner de; Bazotte, Roberto Barbosa; Souza, Romeu Rodrigues de

doi:10.1590/S0004-282X1997000500004

Abstracts

The purpose of this work was to study the neurons of the myenteric plexus of the cecum of rats with chronic streptozotocin-induced diabetes. We used four experimental groups of animals. In groups D2 and D8 animals were killed two and eight months, respectively, after diabetes induction and groups C2 and C8 were used as controls. We carried out whole-mount preparations stanied with Giemsa and NADH-diaphorase. We verified that the diabetes did not alter the shape and disposition of the myenteric ganglia; it provoked decrease on the neuronal density and increase on the incidence of weakly basophilic neurons. The effects of streptozotocin caused dilatation of the cecum still evidenced two months after induction, but no more observed on the eight months after induction. The smaller incidence of neurons in group D8 relative to group C8 was due to the early loss related to the drug toxicity and later to the aging in diabetic condition.

myenteric neurons; diabetes mellitus; myenteric plexus

O objetivo deste trabalho foi estudar os neurônios do plexo mientérico do ceco de ratos, com diabetes mellitus crônico, induzido por estreptozootocina. Utilizamos quatro grupos de animais. Nos grupos D2 e D8, os animais foram mortos, dois e oito meses, respectivamente, após à indução do diabetes e os grupos C2 e C8 foram controles dos grupos experimentais. Realizamos preparados de membrana corados pelo método de Giemsa e NADH-diaforase. Verificamos que o diabetes não alterava a forma e a disposição dos gânglios do plexo mientérico; provocou redução da densidade neuronal e aumento da freqüência de neurônios fracamente basofílicos. Os efeitos da estreptozootocina provocavam dilatação do ceco ainda evidenciada dois meses após a indução, porém não mais verificada no oitavo mês após indução. A menor freqüência de neurônios no grupo D8 em relação ao grupo C8 deve-se a perda inicial relacionada à toxicidade da droga e posteriormente ao envelhecimento em condição de diabetes.

neurônios mientéricos; diabetes mellitus; plexo mientérico

Morphological and quantitative analysis of the neurons of the myenteric plexus of the cecum of streptozotocin-induced diabetic rats

Análise morfológica e quatitativa dos neurônios do plexo mientérico do ceco de ratos diabéticos induzidos por estreptozootocina

Jacqueline Nelisis Zanoni^I; Marcilio Hubner de Miranda-Neto^I; Roberto Barbosa Bazotte^III; Romeu Rodrigues de Souza^IV

^IDepartment of Morphophysiological Sciece of the State University of Maringá, Paraná: Assistant Professor of the Department of Morphophysiological Science

^IIDepartment of Morphophysiological Sciece of the State University of Maringá, Paraná: Adjunct Professor of the Department of Morphophysiological Science

^IIIDepartment of Morphophysiological Sciece of the State University of Maringá, Paraná: Adjunct Professor of the Department of Pharmacy and Pharmacology

^IVAssociate Professor of ICB USP

ABSTRACT

The purpose of this work was to study the neurons of the myenteric plexus of the cecum of rats with chronic streptozotocin-induced diabetes. We used four experimental groups of animals. In groups D2 and D8 animals were killed two and eight months, respectively, after diabetes induction and groups C2 and C8 were used as controls. We carried out whole-mount preparations stanied with Giemsa and NADH-diaphorase. We verified that the diabetes did not alter the shape and disposition of the myenteric ganglia; it provoked decrease on the neuronal density and increase on the incidence of weakly basophilic neurons. The effects of streptozotocin caused dilatation of the cecum still evidenced two months after induction, but no more observed on the eight months after induction. The smaller incidence of neurons in group D8 relative to group C8 was due to the early loss related to the drug toxicity and later to the aging in diabetic condition.

Key words: myenteric neurons, diabetes mellitus, myenteric plexus.

RESUMO

O objetivo deste trabalho foi estudar os neurônios do plexo mientérico do ceco de ratos, com diabetes mellitus crônico, induzido por estreptozootocina. Utilizamos quatro grupos de animais. Nos grupos D2 e D8, os animais foram mortos, dois e oito meses, respectivamente, após à indução do diabetes e os grupos C2 e C8 foram controles dos grupos experimentais. Realizamos preparados de membrana corados pelo método de Giemsa e NADH-diaforase. Verificamos que o diabetes não alterava a forma e a disposição dos gânglios do plexo mientérico; provocou redução da densidade neuronal e aumento da freqüência de neurônios fracamente basofílicos. Os efeitos da estreptozootocina provocavam dilatação do ceco ainda evidenciada dois meses após a indução, porém não mais verificada no oitavo mês após indução. A menor freqüência de neurônios no grupo D8 em relação ao grupo C8 deve-se a perda inicial relacionada à toxicidade da droga e posteriormente ao envelhecimento em condição de diabetes.

Palavras-chave: neurônios mientéricos, diabetes mellitus, plexo mientérico.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Aceite: 29-julho-1997.

Dr. Marcilio Hubner de Miranda Neto • Universidade Estadual de Maringá - Avenida Colombo 5790 Bloco H-79 Sala 01- 87020-900 Maringá PR- Brasil.

This paper is part of the M.A. Dissertation of J.N. Zanoni.

1. Barbosa AJA. Auerbach's plexus of the albino rat: I. Quantitative study of the ganglia and nerve cells in the caecum and colon. Rev Bras Pesq Méd Biol 1973;6:253-262.
2. Barbosa AJA. Técnica histológica para gânglios nervosos intramurais em preparados espessos. Rev Bras Pesq Méd Biol 1978;11:95-97.
3. Belai A, Lincoln J, Milner P, Burnstock G. Differential effect of streptozotocin-induced diabetes on the ileum and distal colon. Gastroenterology 1991;100:1024-1032.
4. Bor-seng shu E, Chadi G, Bor-Jiun-Shu F, Ferraz-de-Carvalho CA, Souza RR. Myenteric neurons of the mouse small intestine morphometry and acetycholinesterase activity. Braz J Med Biol Res 1994;27:101-108.
5. Butow NC. Estudo morfológico e quantitativo do plexo mientérico do duodeno de ratos diabéticos tratados com estreptozootocina. Dissertação de Mestrado, Universidade Estadual de Maringá. Maringá, 1996.
6. Clements RS, Bell DSH. Diabetic neuropathy: peripheral and autonomic syndromes. Diabetic Neuropathy 1982;71:50-67.
7. Diane AR, Grogan DM, Yates ME, Risinger DL, Gerritsen GC. Radiologic abnormalities and autonomic neuropathology in the digestive tract of the ketonuric diabetic Chinese hamster. Diabetologia 1979;17:33-40.
8. Duchen LW, Anjorin MB, Watkins PJ, Mackay JD. Pathology of autonomic neuropathy in diabetes mellitus. Ann Intern Med 1980;92:301-303.
9. Feldmann M, Corbett DB, Ramsey EJ, Walsh JH, Richardson CT. Abnormal gastric function in longstanding insulin-dependent diabetic patients. Gastroenterology 1979;77:12-17.
10. Gabella G. Detection of nerve cells by a histochemical technique. Experientia 1969;25:218-219.
11. Gabella G. Neuron size and number in the myenteric plexus of the newborn and adult rat. J Anat 1971; 109:81-95.
12. Gabella G. Fine structure of the myenteric plexus in the Guinea-pig ileum. J Anat 1972,11:69-97.
13. Gabella G. Innervation of the gastrointestinal tract. Internat Rev Cytol 1979;59:129-193.
14. Gabella G. The number of neurons in the small intestine of mice, Guinea-pigs and sheep. Neuroscience 1987;22:737-752.
15. Gabella G. Fall in the number of myenteric neurons in Asian guinea-pigs. Gastroenterology 1989;96:1487-1493.
16. Hensley GT, Soergel KH. Neuropathologic findings in diabetic diarrhea. Arch Pathol 1968;85:587-597.
17. Hernandez L. Estudo morfológico da mucosa e do corpo celular dos neurônios do plexo mientérico do íleo de ratos com diabetes mellitus induzido por estreptozootocina. Dissertação de Mestrado, Universidade Estadual de Maringá. Maringá, 1994.
18. Hosking DJ, Bennett T, Hampton JR. Diabetic neutonomic neuropathy. Diabetes 1978;27:1043-1055.
19. Iber FL, Parveen S, Vandrunem M, et al.. Relation of symptoms to impaired stomach, small bowel, and colon motility in long-standing diabetes. Dig Dis Sci 1993;38:45-50.
20. Katz LA, Spiro HM. Gastrointestinal manifestations of diabetes. N Engl J Med 1966;15:1350-1360.
21. Kristal BS, Yu BP. An emerging hypothesis: synergistic induction of aging by free radicals and Maillard reactions. J Gastroenterol Biol Sci 1992;47:107-114.
22. Monckton G, Pehowich E. Autonomic neuropathy in the streptozotocin diabetic rat. J Can Neurol Sci 1980;7:135-142.
23. Nowak TV, Harrigton B, Kalbfleisch J. Adaption of cholinergic enteric neuromuscular transmission in diabetic rat small intestine. Diabetes 1990;39:891-897.
24. Santer RM, Baker DM. Enteric neurons number and size in Auerbach's plexus in the small and large intestine of adult and aged rats. J Auton Nerv Syst 1988;25:57-59.
25. Souza RR, Morateli HB, Borges N, Liberti EA. Age-induced nerve cell loss in the myenteric plexus of the small intestine in man. Int J Exp Clin Gerontol 1993;39:183-188.
26. Yang R, Arem R, Chan L. Gastrointestinal tract complications of diabetes mellitus: pathophysiology and management Arch Intern Med 1984;144:1251-125.

Publication Dates

Publication in this collection
07 Oct 2010
Date of issue
1997

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Barbosa AJA. Auerbach's plexus of the albino rat: I. Quantitative study of the ganglia and nerve cells in the caecum and colon. Rev Bras Pesq Méd Biol 1973;6:253-262.

[2] 2. Barbosa AJA. Técnica histológica para gânglios nervosos intramurais em preparados espessos. Rev Bras Pesq Méd Biol 1978;11:95-97.

[3] 3. Belai A, Lincoln J, Milner P, Burnstock G. Differential effect of streptozotocin-induced diabetes on the ileum and distal colon. Gastroenterology 1991;100:1024-1032.

[4] 4. Bor-seng shu E, Chadi G, Bor-Jiun-Shu F, Ferraz-de-Carvalho CA, Souza RR. Myenteric neurons of the mouse small intestine morphometry and acetycholinesterase activity. Braz J Med Biol Res 1994;27:101-108.

[5] 5. Butow NC. Estudo morfológico e quantitativo do plexo mientérico do duodeno de ratos diabéticos tratados com estreptozootocina. Dissertação de Mestrado, Universidade Estadual de Maringá. Maringá, 1996.

[6] 6. Clements RS, Bell DSH. Diabetic neuropathy: peripheral and autonomic syndromes. Diabetic Neuropathy 1982;71:50-67.

[7] 7. Diane AR, Grogan DM, Yates ME, Risinger DL, Gerritsen GC. Radiologic abnormalities and autonomic neuropathology in the digestive tract of the ketonuric diabetic Chinese hamster. Diabetologia 1979;17:33-40.

[8] 8. Duchen LW, Anjorin MB, Watkins PJ, Mackay JD. Pathology of autonomic neuropathy in diabetes mellitus. Ann Intern Med 1980;92:301-303.

[9] 9. Feldmann M, Corbett DB, Ramsey EJ, Walsh JH, Richardson CT. Abnormal gastric function in longstanding insulin-dependent diabetic patients. Gastroenterology 1979;77:12-17.

[10] 10. Gabella G. Detection of nerve cells by a histochemical technique. Experientia 1969;25:218-219.

[11] 11. Gabella G. Neuron size and number in the myenteric plexus of the newborn and adult rat. J Anat 1971; 109:81-95.

[12] 12. Gabella G. Fine structure of the myenteric plexus in the Guinea-pig ileum. J Anat 1972,11:69-97.

[13] 13. Gabella G. Innervation of the gastrointestinal tract. Internat Rev Cytol 1979;59:129-193.

[14] 14. Gabella G. The number of neurons in the small intestine of mice, Guinea-pigs and sheep. Neuroscience 1987;22:737-752.

[15] 15. Gabella G. Fall in the number of myenteric neurons in Asian guinea-pigs. Gastroenterology 1989;96:1487-1493.

[16] 16. Hensley GT, Soergel KH. Neuropathologic findings in diabetic diarrhea. Arch Pathol 1968;85:587-597.

[17] 17. Hernandez L. Estudo morfológico da mucosa e do corpo celular dos neurônios do plexo mientérico do íleo de ratos com diabetes mellitus induzido por estreptozootocina. Dissertação de Mestrado, Universidade Estadual de Maringá. Maringá, 1994.

[18] 18. Hosking DJ, Bennett T, Hampton JR. Diabetic neutonomic neuropathy. Diabetes 1978;27:1043-1055.

[19] 19. Iber FL, Parveen S, Vandrunem M, et al.. Relation of symptoms to impaired stomach, small bowel, and colon motility in long-standing diabetes. Dig Dis Sci 1993;38:45-50.

[20] 20. Katz LA, Spiro HM. Gastrointestinal manifestations of diabetes. N Engl J Med 1966;15:1350-1360.

[21] 21. Kristal BS, Yu BP. An emerging hypothesis: synergistic induction of aging by free radicals and Maillard reactions. J Gastroenterol Biol Sci 1992;47:107-114.

[22] 22. Monckton G, Pehowich E. Autonomic neuropathy in the streptozotocin diabetic rat. J Can Neurol Sci 1980;7:135-142.

[23] 23. Nowak TV, Harrigton B, Kalbfleisch J. Adaption of cholinergic enteric neuromuscular transmission in diabetic rat small intestine. Diabetes 1990;39:891-897.

[24] 24. Santer RM, Baker DM. Enteric neurons number and size in Auerbach's plexus in the small and large intestine of adult and aged rats. J Auton Nerv Syst 1988;25:57-59.

[25] 25. Souza RR, Morateli HB, Borges N, Liberti EA. Age-induced nerve cell loss in the myenteric plexus of the small intestine in man. Int J Exp Clin Gerontol 1993;39:183-188.

[26] 26. Yang R, Arem R, Chan L. Gastrointestinal tract complications of diabetes mellitus: pathophysiology and management Arch Intern Med 1984;144:1251-125.