Services on Demand
- Cited by SciELO
- Access statistics
- Cited by Google
- Similars in SciELO
- Similars in Google
Print version ISSN 0004-282X
On-line version ISSN 1678-4227
Arq. Neuro-Psiquiatr. vol.67 no.1 São Paulo Mar. 2009
Mitochondrial alterations in dynamin 2-related centronuclear myopathy
Alterações mitocondriais na miopatia centronuclear relacionadas a dinamina 2
Edmar ZanoteliI; Naja VerganiII; Yvan CamposII; Mariz VainzofIII; Acary S.B. OliveiraI; Alessandra d'AzzoII
IDepartment of Neurology, UNIFESP-EPM, São Paulo SP, Brazil
IIDepartment of Genetics and Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, USA
IIIHuman Genome Research Center, IB-USP, São Paulo SP, Brazil
Centronuclear myopathy (CNM) is clinically characterized by diffuse involvement of skeletal muscles, with onset mainly in early childhood and a slowly progressive course. The basic histological abnormalities in muscle biopsies are a high percentage of centrally located nuclei of muscle fibers, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers1-3. Three forms of the disease have been recognized that differ in age of onset and severity of the symptoms: The severe X-linked myotubular myopathy, caused by mutations in the MTM1 gene; the childhood onset form; and the juvenile/adult onset form that fully manifests during the third decade of life. In the juvenile/adult onset form, most of the families have an autosomal dominant inheritance, and patients present with a slowly progressive disease, but with mild clinical manifestations2. This form has been associated with mutations in the dynamin 2 gene (DNM2)4. More recently, Nicot et al.5 described in three CNM families with autosomal recessive inheritance mutations in amphiphysin 2 gene, which disrupt the interaction with dynamin 2 protein.
We have now identified an adult-CNM patient with mutation in DNM2 and overt mitochondrial changes in muscle biopsy.
A 50-year-old male, from a large Brazilian family, presented since the age of 30 with slowly progressive muscle weakness associated with impaired capacity to stand up, run and climb the stairs (Fig 1, III-6). Clinical examination confirmed weakness predominantly in the distal part of the limbs (Table). The Gowers' signal was present. He could not walk on his heel. Atrophy of the limbs was evident, especially in the distal parts, as well as tendon hyporeflexia with normal muscular tonus. There was no noticeable weakness of facial or ocular movement; and ptosis was absent. The patient's most consistent complaint was easy fatigue even during regular daily activity.
Other four affected members of his family were also examined (Fig 1) (Table). These patients had similar complaints of fatigue, muscle pain and cramps. All patients presented a slowly progressive disease, and could not walk on their heels. All patients had normal ocular movements. Normal muscular tonus with hyporeflexia was observed in all affected patients. Patients II-6, III-5, and IV-2 presented a clear distal atrophy of the limbs, especially in the lower legs. Neonatal manifestations such as mild hypotonia, suction and cry weakness were described only for the patient III-5; however the motor development was normal.
Deltoid muscle biopsy from patient III-6, performed at the age of 50, showed increased number of central nuclei (more than 80% of the fibers), intense variation in fiber size, moderate increase of the endomysial and perimysial connective tissue and intense perifascicular fat infiltration (Fig 2A). The histochemical study revealed predominance and hypotrophy of type 1 fibers. Spoke-like appearance was observed in most of the fibers (Fig 2B). About 4% of the fibers showed a ragged-red aspect (Fig 2C). Additionally, there were fibers with increased SDH activity (Fig 2D) and reduced or absent COX activity (about 5%) (Fig 2EF). Inflammation and necrosis were not observed. The muscle biopsy from another family member (III-5), performed at the age of 35, showed typical characteristics of CNM, but no ragged-red fibers, SDH positive fibers or COX deficient fibers.
The exons and intron-exon boundaries from the middle domain of the DNM2 gene were sequenced, and a heterozygous missense mutation in exon 11 (1393CT), which results in the amino acid substitution R465W, was identified in patients II-6, III-5, III-6, III-8 and IV-2.
The muscle biopsies and the DNA from the patients were obtained with informed consent following protocols approved by the ethical committee from the Institution.
We report here a case of adult onset CNM with mutation in the DNM2 gene and additional histological findings typically seen in mitochondrial diseases.
Only a few reports are present in the literature describing mitochondrial changes in CNM patients. In one patient, crystalline inclusions were found in mitochondria adjacent to the centralized nuclei6. In another case, mitochondrial abnormalities, including reduction of the number of central and subsarcolemmal mitochondria, reduction of cristae, and mitochondrial remnants within lysosomes, were observed7, and Naumann et al.8 described two typical adult CNM patients with ragged-red fibers and fibers with absence of COX staining in the muscle biopsy. Although no molecular analyses were done on any of these patients, their histological alterations were similar to those described in CNM patients carrying mutations in the DNM2 gene, i.e. fiber type variability, type 1 atrophy and spoke-like appearance4. Furthermore, these patients also had a late onset form of the disease. Based on our findings, we believe that also those early cases might have carried mutations in the DNM2 gene, indicating that the combined occurrence of CNM with DNM2 mutations and mitochondrial changes could be more frequent than recognized until now. More recently, ragged red fibers were identified in the muscle biopsy from a patient with DNM2-related CNM (DNM2-CNM)9.
We could speculate that altered DNM2 protein makes the skeletal muscle of CNM patients more prone to develop a mitochondrial dysfunction as they age. This could explain the lack of mitochondrial changes in another affected member of the same family, who was biopsied at the age of 35. In addition, we cannot exclude interfamilial variability, which is commonly observed in muscle diseases.
Bitoun et al.4 demonstrated that in DNM2-CNM the DNM2 protein presents an aberrant centrosomal localization in vitro, indicating that DNM2 mutations might cause CNM by interfering with centrosome function. Dynamins are large GTPases involved in membrane trafficking that act as mechanochemical scaffolding molecules that can hydrolyze GTP to alter biological membranes10. Among the dynamin family, the 100-kDa GTPase DNM2 is known to be involved in endocytosis and membrane trafficking11, actin reorganization12, and centrosome cohesion13. Furthermore, dynamin-related GTPase has been suggested to be involved in the processes of mitochondrial fission and fusion14,15, both of which are known to play a key role in cell division and differentiation. Considering the broad range of cellular mechanisms in which DNM2 is involved, it is conceivable that an altered DNM2 protein could also interfere with the correct formation and/or function of the mitochondria in affected patients, especially during the aging. We conclude that muscle biopsy from DNM2-CNM patients should be investigated for associated mitochondrial changes.
1. Spiro AJ, Shy GM, Gonatas NK. Myotubular myopathy. Arch Neurol 1966;14:1-14. [ Links ]
2. Goebel HH, Meinck HM, Reinecke M, Schimrigk K, Mielke U. Centronuclear myopathy with special consideration of the adult form. Eur Neurol 1984;23:425-434. [ Links ]
3. Zanoteli E, Oliveira ASB, Schmidt B, Gabbai AA. Centronuclear myopathy: clinical aspects of ten Brazilian patients with childhood onset. J Neurol Sci 1998;158:76-82. [ Links ]
4. Bitoun M, Maugenre S, Jeannet PY, et al. Mutations in dynamin 2 cause dominant centronuclear myopathy. Nat Genet 2005;37:1207-1209. [ Links ]
5. Nicot AS, Toussaint A, Tosch V, et al. Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy. Nat Genet 2007;39:1134-1139. [ Links ]
6. Canal N, Comi GC, Comola M, Testa D, Mora M, Cornelio F. Centronuclear myopathy with unusual mitochondrial abnormalities. Clin Neuropathol 1985;4:23-27. [ Links ]
7. Hulsmann N, Gullotta F, Okur H. Cytopathology of an unusual case of centronuclear myopathy: light- and electron-microscopic investigations. J Neurol Sci 1981;50:311-333. [ Links ]
8. Naumann M, Reiners K, Gold R, et al. Mitochondrial dysfunction in adult-onset myopathies with structural abnormalities. Acta Neuropathol 1995;89:152-157. [ Links ]
9. Pirra L, Dubrovsky A, Bitoun M, et al. Ragged red fibres finding in muscle biopsy of dynamin 2-related centronuclear myopathy. Neuromuscul Disord 2007;17:881-882. [ Links ]
10. Praefcke GJ, McMahon HT. The dynamin superfamily: universal membrane tubulation and fission molecules? Nat Rev Mol Cell Biol 2004;5:133-147. [ Links ]
11. Jones SM, Howell KE, Henley JR, Cao H, McNiven MA. Role of dynamin in the formation of transport vesicles from the trans-Golgi network. Science 1998;279:573-577. [ Links ]
12. Orth JD, McNiven MA. Dynamin at the actin-membrane interface. Curr Opin Cell Biol 2003;15:31-39. [ Links ]
13. Thompson HM, Cao H, Chen J, Euteneuer U, McNiven MA. Dynamin 2 binds gamma-tubulin and participates in centrosome cohesion. Nat Cell Biol 2004;6:335-342. [ Links ]
14. Barsoum MJ, Yuan H, Gerencser AA, et al. Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons. EMBO 2006;25:3900-3911. [ Links ]
15. van der Bliek AM. A mitochondrial division apparatus takes shape. J Cell Biol 2000;151(2):F1-4. [ Links ]
Received 17 September 2008. Accepted 12 December 2008.
E.Z. and A.d'A. are supported by the National Institutes of Health Grant AR 0499867, the Cancer Center (CORE) support grant CA021765, the Assisi Foundation of Memphis, and the American Lebanese Syrian Associated Charities (ALSAC). M.V. is supported by FAPESP and CNPq
Dr. Edmar Zanoteli Department of Neurology / UNIFESP-EPM - Rua Botucatu 740 - 04023-900 São Paulo SP - Brasil. E-mail: email@example.com