Print version ISSN 0004-282X
On-line version ISSN 1678-4227
Arq. Neuro-Psiquiatr. vol.67 no.2b São Paulo June 2009
Diffusion tensor imaging of the cervical spinal cord of patients with relapsing-remising multiple sclerosis: A study of 41 cases
Imagens por tensor de difusão da medula cervical de pacientes com esclerose múltipla remitente-recorrente: estudo de 41 casos
L. Celso Hygino Cruz Jr.I; Romeu C. DominguesII; Emerson L. GasparettoIII
IClínicas Multi-Imagem, CDPI e Departamento de Radiologia da Faculdade de Medicina da Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ, Brazil: Médico Radiologista das Clínicas Multi-Imagem e CDPI, e Mestrando do Programa de Pós-Graduação em Medicina (Radiologia) da Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ, Brazil
IIClínicas Multi-Imagem, CDPI e Departamento de Radiologia da Faculdade de Medicina da Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ, Brazil: Diretor Médico e Médico Radiologista das Clínicas Multi-Imagem e CDPI, Rio de Janeiro RJ, Brazil
IIIClínicas Multi-Imagem, CDPI e Departamento de Radiologia da Faculdade de Medicina da Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ, Brazil: Médico Radiologista das Clínicas Multi-Imagem e CDPI, e Professor Adjunto do Departamento de Radiologia da faculdade de Medicina da Universidade Federal do Rio de Janeiro RJ, Brazil
OBJECTIVE: To evaluate the fractional anisotropy (FA) values of the multiple sclerosis (MS) plaques and normal-appearing cervical spinal cord (NASC) by diffusion tensor MRI imaging (DTI).
METHOD: Forty-one patients with relapsing-remising MS and 37 controls were evaluated. All MRI exams were performed using a conventional protocol, as well as diffusion tensor MR imaging. Regions of interest were placed within the spinal cord lesions and in the normal appearing spinal cord adjacent to the plaque.
RESULTS: The FA values were statistically reduced in the plaques compared to the surrounding NASC and to equivalent location in controls. A reduction in FA values was also observed in the spinal cord of MS patients without visible lesions on T2WI.
CONCLUSION: We observed reduced fractional anisotropy in the demyelinating plaques and in the NASC of MS patients, corroborating the hypothesis that the histological extension of the MS lesions is more severe than the abnormalities seen in the conventional MRI sequences.
Key words: multiple sclerosis, magnetic resonance imaging, diffusion tensor imaging.
OBJETIVO: Avaliar os valores da anisotropia fracionada (FA) em pacientes com esclerose múltipla (EM) nas placas e na medula espinhal aparentemente normal (MEAN).
MÉTODO: Quarenta e um pacientes com EM remitente-recorrente e 37 controles foram examinados. Todos os exames foram realizados com protocolo convencional, assim como imagens por tensor de difusão. Regiões de interesse foram definidas nas placas da medula espinhal e na MEAN ao redor das placas.
RESULTADOS: Os valores de FA estavam significativamente reduzidos nas placas, comparados à MEAN ao redor e às regiões equivalentes dos controles. Redução dos valores de FA também foi demonstrada na medula espinhal de pacientes com EM sem lesões visíveis nas imagens de RM pesadas em T2.
CONCLUSÃO: Observamos redução dos valores de anisotropia fracionada nas placas de desmielinização e na MEAN, corroborando a hipótese de que a extensão histológica das lesões na EM é maior que as alterações de sinal vistas nas seqüências convencionais de ressonância magnética.
Palavras-chave: esclerose múltipla, ressonância magnética, imagens por tensor de difusão.
The involvement of the spinal cord is a common finding in patients with multiple sclerosis (MS). Post-mortem and magnetic resonance (MR) imaging studies have shown cord lesions in up to 90% of these patients1-4. In addition, the cord involvement is likely to contribute to the level of disability, particularly locomotor impairment, which is one of the main clinical hallmarks of the disease1,2. However, correlation between severity of the lesions seen on conventional MR imaging and clinical disability was not yet achieved. This divergence could represent the inability of conventional MR imaging to quantify accurately the degree of cord tissue damage3. Post-mortem studies have shown that the cord damage is more extensive than the macroscopic lesions seen on conventional MR imaging4. In addition, histopathologic studies have demonstrated spinal cord lesions in patients with defined diagnosis of MS, but with normal spinal MR imaging5. Moreover, advanced MR imaging techniques, such as diffusion tensor imaging (DTI), have shown involvement of the brain white matter in areas with normal signal on conventional MR imaging6-8.
The diffusion tensor imaging requires the measurement of the MR signal with diffusion sensitization along six or more non-collinear directions. It allows the determination of three perpendicular eigenvectors, whose magnitudes are given by three corresponding eigenvalues. Various mathematical combinations of these three vectors allow the derivation of a number of diffusion indices. The main advantage of using diffusion tensor indices in clinical studies is that they are reliable, quantitative, and objective measures of the diffusion properties in the human brain. In white matter, water molecules diffuse preferentially in the direction parallel to axons. This property, termed diffusion anisotropy, may be quantified by the fractional anisotropy (FA) indices. FA increases with anisotropy and provides the most detailed spatial depiction of anisotropic areas9,10.
Several studies have been investigating the spinal cord of patients with multiple sclerosis using DTI11-14. The results have demonstrated that the cervical cord FA values are significantly lower in MS patients compared to controls. In addition, FA and mean diffusivity values have correlated with the degree of disability in these patients. However, there is still a lack of studies investigating the DTI findings in MS plaques and normal-appearing spinal cord (NASC) (no abnormal signal on T2-weighted MR images) of patients with multiple sclerosis.
The aim of this study was to assess the fractional anisotropy values of the MS plaques and normal-appearing cervical spinal cord in patients with MS using diffusion tensor MR imaging.
We retrospectively studied a cohort of 41 patients with MS (31 female and 10 male, mean age 33.4 years-old) and 37 age- and sex-matched healthy volunteers (28 female and nine male, mean age 31 years-old). All patients had the relapsing-remising form of MS and the diagnosis of was defined based on McDonald' criteria15. All patients had been receiving medication for various periods of time when the MR imaging data was acquired. The patients and controls signed informed consent and the institutional review board approved the study.
The exams were performed in a 1.5T scanner (Magnetom Avanto, Siemens, Germany), with 8 channels head coil. All patients underwent the conventional MR imaging protocol, including sagittal T1-weighted images (repetition time (TR)/echo time (TE) 4410/98ms, field of view (FOV) 240 mm, matrix 320×320 mm and slice thickness 3 mm with 30% of interval), sagittal T2-weighted images (TR/TE 4410/98 ms, FOV 240 mm, matrix 320×320 mm and slice thickness 3 mm with 30% of interval) and axial T2*-weighted images (TR/TE 4410/98 ms, FOV 240 mm, matrix 320×320 mm and slice thickness 3 mm with 30% of interval).
The DTI sequences employed gradient pulses in twelve different directions in the axial (TR/TE 3200/80 ms, FOV 225×225 mm, matrix 128×128 mm, slice thickness 3 mm with 30% of interval) and sagittal planes (TR/TE 2800/90 ms, FOV 280×280 mm, matrix 192×192 mm, slice thickness 3 mm with no interval).
The DTI data was post-processed using DTI Task Card software (Massachusetts General Hospital, Boston, United States), and FA maps were calculated. For the placement of regions of interest (ROI), the patients with MS were divided in two groups: group one (n=29) patients with areas of high signal on T2-weighted images (plaques) in the cervical spinal cord and group two (n=12) patients with no evidence of abnormal signal on T2-weighted images in the cervical spinal cord. In the group one the ROIs were placed on the plaques, around the plaques and on the NASC distant more than 10 mm from the plaque, using sagittal FA maps (mean size of the ROI 0.30 mm2) (Fig 1). In the controls of group one the ROI was positioned on the center of the spinal cord at the level of C2-C3, and included most of the cord tissue at this level. In the group two and its controls, four ROIs were placed at the level of C2-C3 in every patient on the following regions: right anterior horn, right posterior horn, left anterior horn and left posterior horn (mean size of the ROI 0.08 mm2) (Fig 2).
Regarding the group one, which included patients with MS and abnormal spinal cord MR imaging, the statistical analysis compared the FA values of the plaque, periplaque and NASC with the values obtained at the central region of the controls' spinal cord at the level of C2-C3. In the group two, which included patients with MS and normal spinal cord MR imaging, the comparison considered the FA values of study and control groups that were measured on different regions of the spinal cord (right anterior horn, right posterior horn, left anterior horn and left posterior horn) at the level of C2-C3. The statistical treatment was performed with the exact Mann-Whitney test, and P values of less than 0.05 were considered statistically significant.
The FA values obtained thought DTI of the cervical spinal cord of patients with MS, who showed abnormal high signal on T2-weighted images (plaques) are shown in the Table 1. The FA values were significantly lower in the plaques compared to the periplaque region, NASC and controls (p<0.001). In addition, the FA values in the periplaque region were also lower than the NASC and controls (p<0.001). Finally, the FA mean values in the NASC were lower than the values obtained in the control group (p<0.05).
The Table 2 shows the FA values of the cervical spinal cord of patients with MS, who had normal conventional MR imaging. The FA values obtained in all the regions of NASC of patients with MS were significantly lower than controls (p<0.05). Comparing only the patients with MS and NASC, there was no significant difference between the FA values obtained in the posterior and anterior horns at the level of C2-C3 (p>0.05).
In this study, we retrospectively evaluated a cohort of 41 patients with relapsing-remising MS and compared the DTI findings with an age- and gender-matched control group. Similar to other studies11-14, in this series the fractional anisotropy in the plaques, periplaque region and NASC more than 10 mm distant from the plaques was significantly lower than controls. Also, the FA values obtained in our series were robust and comparable to the previous studies11. In addition, when evaluating the FA of the NASC at the level of C2-C3, both anterior and posterior horns showed reduced FA values. However, no significant differences were seen when comparing the fractional anisotropy of the anterior and posterior horns.
Multiple sclerosis is a chronic inflammatory disease that causes demyelination and axonal loss in both brain and spinal cord. Spinal cord lesions demonstrated with MR imaging have been included in revised McDonald criteria of MS15, ascertain that these findings can be used to demonstrate disease dissemination in time and space. MS spinal cord abnormalities may consist of focal well-demarcated or diffuse poorly demarcated lesions, and up to 97% of the MS patients demonstrate spinal cord abnormalities on MR imaging16. Currently, spinal cord MR imaging studies focus on the presence of lesions visible on T2, short tau inversion recovery (STIR) or proton density sequences, which are secondary to demyelination17. However, previous authors have failed to prove the relation between the spinal cord MR imaging abnormalities on the conventional sequences and the clinical disability16,18,19. Advanced MR imaging techniques, such as proton spectroscopy and diffusion tensor imaging, have also been studied with some success when aiming to demonstrate a correlation between the imaging findings and the clinical status20. Also, the DTI have demonstrated fractional anisotropy abnormalities in the brain white matter7,8 and in the spinal cord11,12, even in regions with normal signal on T2-weighted images, defining the well-known terms called "normal appearing white matter" and "normal appearing spinal cord".
The diffusion tensor imaging is a recent MR imaging technique that allows measurement of magnitude and directionally of water diffusion in tissue, providing a quantitative method for assessment of the integrity of white matter fiber tracts9,10. Previous studies with DTI have demonstrated reduced fractional anisotropy in the demyelinating plaques as well as in the normal appearing white matter in the brain of MS patients, despite any abnormality in the conventional MR imaging sequences, including T2-weighted image and FLAIR6-8.
Several authors had evaluated the spinal cord of patients with multiple sclerosis using diffusion tensor MR imaging11-14. Agosta et al.13 investigated 24 patients with progressive MS and demonstrated reduction of the cross-sectional area and average cord fractional anisotropy. However, the DTI parameters showed no correlation with conventional and DTI MR findings in the brain. They suggested that the DTI of the cervical cord could quantify the extent of the diffuse cord pathology in MS, but these findings are independent of the brain damage. Hesseltine et al.12 studied 24 patients with relapsing-remising MS, measuring the FA in the anterior, posterior, lateral and central areas of the normal appearing cervical spinal cord at the level of C2-C3. The fractional anisotropy was reduced in the lateral, posterior and central regions, and the authors suggested that the DTI could be useful in detecting spinal cord occult lesions, predicting clinical course and monitoring disease progression. In addition, Ohgiya et al.11 also demonstrated reduction of the fractional anisotropy in the C2-C3, C3-C4 and C4-C5 spinal cord levels in 21 patients with MS. The mean FA was 0.441 in the plaques, 0.542 in the NASC and 0.739 in the controls. Thus, these studies point to the potential use of the diffusion tensor MR imaging for the evaluation of the spinal cord plaques and NASC of patient with MS, mainly for identification of occult lesions and probably for the evaluation of disease progression.
Although initial reports suggest advantages of DTI in the evaluation of spinal cord in MS patients, they are single-center preliminarily results and should be cautiously interpreted. The DTI has several technical limitations, mainly related to low signal-to-noise ratio and movement artifacts associated to breath and pulsation. Also, the technical limitations of the spinal cord DTI are even more important, as there is a small amount of tissue (spinal cord) contributing for the signal and a large amount of tissue (bone and liquor) causing artifacts. Finally, a limitation of our study was the selection of patients with MS referred from different physicians, and thus being submitted to diverse treatments. As a result, we couldn't make correlations between the DTI data and treatment response or follow-up. Nevertheless, these initial results are promising and needs further investigations.
In conclusion, DTI seems to offer the possibility of adding important information for the evaluation of MS patients. In this series of relapsing-remising MS patients, we observed reduced fractional anisotropy in the demyelinating plaques and in the NASC, corroborating the hypothesis that the histological extension of the MS lesions is more severe than the abnormalities seen in the conventional MR imaging sequences. While the spinal cord MR imaging is improving and gaining importance in the clinical evaluation of MS patients, improvements in the MR imaging acquisition and analysis are welcome to provide even more sensible imaging evaluation of the spinal cord. The method herein assessed is capable to depict subtle and diffuse changes in the microarchitecture of spinal cord in MS patients despite any visible lesions in the conventional MR sequences. As a result, fractional anisotropy measurement makes possible the assessment of early stage spinal cord involvement. Further studies can confirm the role of this advanced MR imaging technique for predicting the real extension of the demyelinating disease, as well as for evaluating the disease progression and treatment response.
1. Zivadinov R, Banas AC, Yella V, Abdelrahman N, Weinstock-Guttman B, Dwyer MG. Comparison of three different methods for measurement of cervical cord atrophy in multiple sclerosis. AJNR Am J Neuroradiol 2008;29:319-325. [ Links ]
2. Morales Rde R, Morales Nde M, Rocha FC, Fenelon SB, Pinto Rde M, Silva CH. Health-related quality of life in multiple sclerosis. Arq Neuropsiquiatr 2007;65:454-460. [ Links ]
3. Bakshi R, Thompson AJ, Rocca MA, et al. MRI in multiple sclerosis: current status and future prospects. Lancet Neurol 2008;7:615-625. [ Links ]
4. Gilmore CP, Bö L, Owens T, Lowe J, Esirimm, Evangelou N. Spinal cord gray matter demyelination in multiple sclerosis-a novel pattern of residual plaque morphology. Brain Pathol 2006;16:202-208. [ Links ]
5. Bot JC, Blezer EL, Kamphorst W, et al. The spinal cord in multiple sclerosis: relationship of high-spatial-resolution quantitative MR imaging findings to histopathologic results. Radiology 2004;233:531-540. [ Links ]
6. Rocca MA, Iannucci G, Rovaris M, Comi G, Filippi M. Occult tissue damage in patients with primary progressive multiple sclerosis is independent of T2-visible lesions--a diffusion tensor MR study. J Neurol 2003;250:456-460. [ Links ]
7. Rovaris M, Gass A, Bammer R, et al. Diffusion MRI in multiple sclerosis. Neurology 2005;65:1526-1532. [ Links ]
8. Andrade RE, Gasparetto EL, Cruz LC Jr, et al. Evaluation of white matter in patients with multiple sclerosis through diffusion tensor magnetic resonance imaging. Arq Neuropsiquiatr 2007;65:561-564. [ Links ]
9. Nucifora PG, Verma R, Lee SK, Melhem ER. Diffusion-tensor MR imaging and tractography: exploring brain microstructure and connectivity. Radiology 2007;245:367-384. [ Links ]
10. Melhem ER, Mori S, Mukundan G, Kraut MA, Pomper MG, van Zijl PC. Diffusion tensor MR imaging of the brain and white matter tractography. AJR Am J Roentgenol 2002;178:3-16. [ Links ]
11. Ohgiya Y, Oka M, Hiwatashi A, et al. Diffusion tensor MR imaging of the cervical spinal cord in patients with multiple sclerosis. Eur Radiol 2007;17:2499-2504. [ Links ]
12. Hesseltine SM, Law M, Babb J, et al. Diffusion tensor imaging in multiple sclerosis: assessment of regional differences in the axial plane within normal-appearing cervical spinal cord. AJNR Am J Neuroradiol 2006;27:1189-1193. [ Links ]
13. Agosta F, Benedetti B, Rocca MA, et al. Quantification of cervical cord pathology in primary progressive MS using diffusion tensor MRI. Neurology 2005;64:631-635. [ Links ]
14. Valsasina P, Rocca MA, Agosta F, et al. Mean diffusivity and fractional anisotropy histogram analysis of the cervical cord in MS patients. Neuroimage 2005;26:822-828. [ Links ]
15. Polman CH, Reingold SC, Edan G, et al. Diagnostic criteria for multiple sclerosis: 2005 revisions to the "McDonald Criteria". Ann Neurol 2005;58:840-846. [ Links ]
16. Lycklama à Nijeholt GJ, Barkhof F, Scheltens P, et al. MR of the spinal cord in multiple sclerosis: relation to clinical subtype and disability. AJNR Am J Neuroradiol 1997;18:1041-1048. [ Links ]
17. Nijeholt GJ, Bergers E, Kamphorst W, et al. Post-mortem high-resolution MRI of the spinal cord in multiple sclerosis: a correlative study with conventional MRI, histopathology and clinical phenotype. Brain 2001;124:154-166. [ Links ]
18. Bergers E, Bot JC, van der Valk P, et al. Diffuse signal abnormalities in the spinal cord in multiple sclerosis: direct postmortem in situ magnetic resonance imaging correlated with in vitro high-resolution magnetic resonance imaging and histopathology. Ann Neurol 2002;51:652-656. [ Links ]
19. Fu L, Matthews PM, De Stefano N, et al. Imaging axonal damage of normal-appearing white matter in multiple sclerosis. Brain 1998;121:103-113. [ Links ]
20. Reich DS, Zackowski KM, Gordon-Lipkin EM, etl al. Corticospinal tract abnormalities are associated with weakness in multiple sclerosis. AJNR Am J Neuroradiol 2008;29:333-339. [ Links ]
Received 2 October 2008, received in final form 5 January 2009.
Accepted 30 March 2009.
Dr. Emerson L. Gasparetto - Avenida das Américas 4666/325 - 22640-102 Rio de Janeiro RJ - Brasil. E-mail: email@example.com
This study was supported by the FAPERJ - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro and CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico