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versão impressa ISSN 0004-282X
Arq. Neuro-Psiquiatr. vol.70 no.5 São Paulo maio 2012
VIEWS AND REVIEWS
Fisiopatologia da meningite ocasionada pelo Streptococcus pneumoniae e novas possibilidades terapêuticas adjuvantes
Tatiana BarichelloI; Jaqueline S. GenerosoI; Allan CollodelI; Ana Paula MoreiraI; Sérgio Monteiro de AlmeidaII
ILaboratório de Microbiologia Experimental e Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma SC, Brazil
IILaboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná e Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brazil
Pneumococcal meningitis is a life-threatening disease characterized by an acute purulent infection affecting piamater, arachnoid and the subarachnoid space. The intense inflammatory host's response is potentially fatal and contributes to the neurological sequelae. Streptococcus pneumoniae colonizes the nasopharynx, followed by bacteremia, microbial invasion and blood-brain barrier traversal. S. pneumoniae is recognized by antigen-presenting cells through the binding of Toll-like receptors inducing the activation of factor nuclear kappa B or mitogen-activated protein kinase pathways and subsequent up-regulation of lymphocyte populations and expression of numerous proteins involved in inflammation and immune response. Many brain cells can produce cytokines, chemokines and others pro-inflammatory molecules in response to bacteria stimuli, as consequence, polymorphonuclear are attracted, activated and released in large amounts of superoxide anion and nitric oxide, leading to the peroxynitrite formation, generating oxidative stress. This cascade leads to lipid peroxidation, mitochondrial damage, blood-brain barrier breakdown contributing to cell injury during pneumococcal meningitis.
Key words: Streptococcus pneumoniae, meningitis, cytokines, chemokines, oxidative stress.
A meningite pneumocócica é doença potencialmente fatal caracterizada por infecção aguda purulenta que afeta a pia-máter, a aracnoide e o espaço subaracnoide. A resposta inflamatória do hospedeiro é potencialmente fatal e contribui para as sequelas neurológicas. O processo inicia-se com a colonização da nasofaringe pelo Streptococcus pneumoniae, seguida de invasão, bacteremia e passagem através da barreira hematoencefálica. O S. pneumoniae é reconhecido por células apresentadoras de antígenos através da ligação aos receptores Toll-like. Isto induz a ativação do fator nuclear kappa B ou proteína quinase ativada por mitógenos. Muitas células cerebrais também podem produzir citocinas, quimiocinas e outras moléculas pró-inflamatórias em resposta aos estímulos bacterianos. Como consequência, são atraídos polimorfonucleares, ocorrendo a liberação de grandes quantidades de ânion superóxido e óxido nítrico, o que leva à formação de peroxinitrito e ocasiona o estresse oxidativo. Esta cascata pró-inflamatória leva à peroxidação lipídica, a danos mitocondriais e à ruptura da barreira hematoencefálica, contribuindo para o dano celular em meningite pneumocócica.
Palavras-Chave: Streptococcus pneumoniae, meningite, citocinas, quimiocinas, estresse oxidativo.
Bacterial meningitis is the most common and serious bacterial infection of the central nervous system (CNS), characterized by an acute purulent infection of the piamater, arachnoid and subarachnoid space1. Approximately 1.2 million cases are estimated to occur annually world-wide, resulting in 135,000 deaths2,3. Streptococcus pneumoniae and Neisseria meningitidis are the main etiologic agents responsible for most of the meningitis cases in Europe and in the USA with up to 61% of total cases1. In Brazil, in 2011, among all age groups according to the Sistema de Informação de Agravos de Notificação (SINAN) were confirmed 8,676 meningitis cases, from these 37% (n=3,194) were bacterial meningitis and 41% (n=3,562) were viral meningitis. Among all the bacterial meningitis cases 35% (n=1,133) were meningococcal; 43% (n=1,383) were meningitis by other bacteria and 15% (n=487) by pneumococcus4.
Pneumococcus is the most severe cause of bacterial meningitis resulting in a 20-30% of hospital mortality an up to a 40% rate of intracranial complications such as brain edema, hydrocephalus and intracranial hemorrhage1,5. Even patients with good apparent recovery may present sequelae; moreover, around one-third of the patients have cognitive impairments5. These impairments consist mainly of cognitive slowness, learning impairment, deafness, blindness, neuropsychiatric impairments, cerebral palsy, seizure disorders and mental retardation6. The objective of this paper was to review some aspects of the meningitis pathophysiology caused by S. pneumoniae and discuss some possible therapeutic adjunctive approaches.
MECHANISMS OF BACTERIAL COLONIZATION
The pneumococcus habitat is the human nasopharynx mucosa with a prevalence of about 40% in infants and 15% in adults7. The bacterium is transferred among people by coughing and sneezing. It has to face the natural barrier, the host's immune system and up to 700 different microbial species that can colonize the same niche8,9. S. pneumoniae colonizes the nasopharynx by degradation of the mucus by exoglycosidases such as neuraminidase A, β-galactosidase, β-N-acetylglucosaminidase, and neuraminidase B decreasing mucus viscosity10. S. pneumoniae produces the pneumolysin that is a major exotoxin. It decreases epithelial cell ciliary's beating and enhances bacterial adherence11. Pneumococcus also expresses the enzymes peptidoglycan, N-acetylglucosamine-deacetylase A, and O-acetyltransferase that provides resistance to lysozyme12 and it also produces IgA1 protease, which cleaves IgA, the major class of Ig in secretions, promoting binding to the respiratory mucosa13. S. pneumoniae may transmigrate through the epithelial cells by binding the phosphorylcholine with the receptor of the platelet-activating factor (PAF) or by connecting the pneumococcal choline-binding protein with the epithelial polymeric immunoglobulin receptor, which transports the bacterium to the basal membrane of the host's epithelial cell and may lead to invasive illness8,14.
CENTRAL NERVOUS SYSTEM BACTERIAL INVASION
The CNS protection is formed by bony skull, the leptomeninges, the blood-brain barrier (BBB) and blood-cerebrospinal fluid barrier15. The BBB is constituted of brain microvascular endothelial cells, astrocytes, and pericytes. It maintains the neural microenvironment by regulating the passage of molecules into and out of the brain, and preserves the brain from whatever microorganisms and toxins that comes from the blood16. The S. pneumoniae crosses the BBB and interacts with cell-wall phosphorylcholine, the platelet-activating-factor receptor and cross the BBB without any evidence of intercellular tight-junction disruption or detection of microorganism between cells through transcellular traversal mechanism16. It can also crosses intercellularly by disruption of the interepithelial tight junctions8. The pneumococcus replication within the subarachnoid space occur concurrently with the release of the bacterial products such as peptidoglycan, cell wall fragments that are highly immunogenic and may lead to an increased inflammatory response in the host15. S. pneumoniae is recognized by antigen-presenting cells binding to a pattern recognition receptors. The main pattern recognition receptors involved in initial pneumococcus sensing in the CNS are Toll-like 2 receptors (TRL-2 or CD282) that is recognized by peptidoglycans and lipoteichoic acids17, Toll-like 4 receptors (TRL-4 or CD284) that are recognized by exotoxin pneumolysin18 and the Toll-Like 9 receptors (TRL-9 or CD289), an intracellular pattern recognition receptor that is activated by CpG in bacterial DNA19. Family members of the NOD-like receptors (NLRs), which are intracellular, play essential roles on innate immunity by detecting intracellular pathogen-associated molecular patterns20. When they are activated, they induce the activation of nuclear factor kappa B (NF-κB) or mitogen-activated protein kinase (MAPK) pathways and inflammatory caspases21. TRL-2 and TRL-4 use a common intracellular adapter protein known as myeloid differentiation factor 88 (MyD88)8. MyD88 signals for NF-κB activation and subsequent up-regulation of pro-inflammatory mediators. MyD88-deficient mice displayed a markedly diminished inflammatory host response in the CNS, as an evidenced of the reduced CSF pleocytosis and expression of cytokines, chemokines and complement factors22. Furthermore, MyD88-dependent immune response contributes to hearing loss in experimental pneumococcal meningitis; it is required for mounting a robust host immune response to S. pneumoniae in the CNS23. NF-κB comprises a closely related family to transcription factors, which play a key role on the expression of genes involved in the development of accessory cell and lymphocyte populations, expressing numerous proteins involved in inflammation and immune response24. It is also a transcriptional activator of many genes involved in the pathogenesis of pneumococcal meningitis, such as, TNF-α, IL-1β, inducible nitric oxide synthase and intercellular adhesion molecules25,26 (Figure).
Many brain cells such as astrocytes, glial cells, endothelial cells, ependymal cells, and resident macrophages can produce cytokines and pro-inflammatory molecules in response to bacterial replication and its components27. TNF-α is a 158 amino acid cytokine considered a pro-inflammatory molecule, enhancing the immune response to help speed-up the pathogens elimination and the resolution of the inflammatory challenge28. TNF-α leads to NF-κB activation in the CSF and brain resident cells, which regulates the expression of many pro-inflammatory mediators29. In animal models for pneumococcal meningitis, TNF- α was produced in the first 6 h of the immune response30. Intrathecal administration of TNF-α results in a similar pathophysiological characteristic of bacterial meningitis such as BBB disruption, facilitating bacterial traversal into the CSF31, on the other hand, TNF-α deficient mice increased mortality and spatial memory deficits32. TNF-α is a marker of the acute inflammatory response, however, it's also essential for an adequate host immune response8.
IL-1β is a pro-inflammatory cytokine, produced by perivascular, mononuclear phagocytes, glial cells, and meningeal macrophages that increase the expression of nearly all other cytokines such as TNF-α, IL-6, IFN-γ, and chemokines. IL-1β has potent stimulatory effects on granulocytes white cells; it promotes the adhesion of neutrophils and monocytes in endothelial cells33. IL-1β is found in the CSF of patients with bacterial meningitis34, furthermore, in animal models it was produced in the first 24 h after pneumococcal meningitis induction30, although intrathecal administration of IL-1β did not lead to CSF pleocytosis or brain edema35. However, the mortality was significantly higher and earlier in the course of the disease among IL-1 receptor (IL-1R) gene-deficient mice, demonstrating that endogenous IL-1β is required for an adequate host defense in pneumococcal meningitis36.
IL-6 is produced by monocytes, endothelial cells, and astrocytes primarily in response to IL-1β37. It has predominantly pro-inflammatory effects such as potent inducer of acute-phase proteins, fever and leukocytes38 but it also acts as an anti-inflammatory cytokine, indeed, the lack of IL-6 enhances inflammatory response but decreases vascular permeability in bacterial meningitis39. IL-6 gene-deficient mice showed impaired defense against pneumococcal pneumonia40.
IL-10 is a potent immunosuppressive cytokine, produced by monocytes, macrophages, B and T lymphocytes, brain cells such as neurons and microglia41. Elevated levels have been found in the CSF in patients with bacterial meningitis42, it leads to macrophage and monocyte deactivation and inhibits the cytokines production such as TNF-α and IL-6 and the release of reactive oxygen species43. IL-10 gene-deficient mice were associated with higher levels of TNF-α and IL-6 in animal model of pneumococcal meningitis36.
TGF-β is an anti-inflammatory cytokine that is expressed in neurons and glial cells, maintains T cell tolerance to self or innocuous environmental antigens via its direct effects on the differentiation, homeostasis and regulatory T cells44. It suppresses the production of IL-1β, IL-6 and TNF-α from microglia in vitro45, moreover, endogenous TGF-β suppresses the host defense in the CSF of mice with S. pneumoniae meningitis3. The collective activity of TGF-β and IL-10 ensures a controlled inflammatory response specifically targeting pathogens without evoking excessive immunopathology to the self-tissues44.
Chemokines are chemoattractant cytokines, which play key roles on the accumulation of inflammatory cells in the inflammation site. Increased chemokines levels have been reported in the CSF of patients with bacterial meningitis. IL-8 is produced by wide variety cell types such as monocytes, macrophages through stimuli of the live bacteria, TNF-α, and IL-β. It is important to regulate the acute inflammatory response and it's rapidly synthesized in the inflammation sites with the function to recruit acute inflammatory cells46. In pneumococcal meningitis treatment with a monoclonal antibody to IL-8 given intravenously attenuated the pleocytosis in rabbits. IL-8 plays an important role on the recruitment of neutrophils during experimental pneumococcal meningitis47.
Matrix metalloproteinases (MMPs) is a family Zn2+ and Ca2+ dependent endopeptidases, which is secreted by all cell types in the CNS and serve as effectors of cell migration, tissue remodeling, degrade constituents of the BBB and interacts with the cytokines48. Gelatinases (MMP-2 and MMP-9) have shown to induce BBB breakdown and facilitate leukocyte extravasation in experimental bacterial meningitis49, furthermore, patients with bacterial meningitis present high concentrations of MMP-939 and MMP-8 in the CSF, indeed, high concentrations of MMP-9 were correlated with risk factor for the development of postmeningitis neurological sequelae48. In a rat model, adjuvant treatment with dexamethasone resulted in a lower MMP-9 mRNA50, in addition, combining the MMP and TNF-α inhibitors led to a decrease incidence of the seizures and mortality, furthermore, neuronal necrosis in the cortex and apoptosis in the hippocampus were attenuated in rats submitted by pneumococcal meningitis51.
Bacterial components are recognized by Toll-like receptors or other pathogen recognition receptors that lead to the activation of NF-κB21. It triggers the expression of pro-inflammatory cytokines, as consequence, polymorphonuclear are attracted, activated and released in large amounts superoxide anion (O2-) and nitric oxide (NO), leading to the peroxynitrite formation (ONOO-)52. The release of large amounts of reactive nitrogen species (RNS) and reactive oxygen species (ROS) had been documented in patient's populations, likewise, in animal model by pneumococcal meningitis and might contribute to the development of neuronal damage52. Treatment with superoxide dismutase mimetics and catalase (hydrogen peroxide scavenger) inhibited brain edema formation53,54; antibiotic therapy prevented, in part, the oxidative stress in experimental pneumococcal meningitis55. Brain resident cells produce O2-, H2O2 as part of the host immune response to invasive bacterial infection, in addition, S. pneumoniae itself is also an important source of H2O2, which is not only able to cause direct cytotoxic damage but also reacts with the host's NO to form the highly reactive species ONOO56. Peroxynitrite can crosses membranes, activate the MMPs, leads to DNA damage, protein carbonylation and cause lipid peroxidation52, leading to a membrane integrity loss, energy depletion, contributing to cell injury during pneumococcal meningitis57.
NEURONAL DAMAGE AND TARGETS FOR ADJUNCTIVE THERAPY
Pneumococcal meningitis causes sequelae including sensory-motor deficits, hearing loss, deafness and neuro intellectual impairment, including deficits in learning and memory. These neurofunctional consequences occur in up to 30% of survivors patients5. The neuronal damage is caused by the strong inflammatory reaction and direct effects of the microorganism32. Significant injury during bacterial meningitis arises from mechanisms of neuronal apoptosis, particularly in the hippocampus; in autopsies cases of bacterial meningitis were found apoptosis of neurons in the dentate gyrus58. Apoptosis can involve both the caspase-dependent and the caspase-independent pathway59. The caspase-independent pathway is triggered by the pneumolysin and H2O2 that are produced by S. pneumoniae. The actions of these toxins result in an increase in ROS and calcium, resulting in mitochondrial dysfunction that leads to the release of apoptosis-inducing factor into the cytosol56,59. However, the caspase-dependent pathway occurs later, and pneumococcal cell-wall components trigger the required host inflammatory response from the leukocytes. p53 tumor suppressor protein and ATM protein kinase (ATM) as upstream mediators that converge on the mitochondria to initiate the release of cytochrome c, which is necessary to form the apoptosome forming apoptotic protease activating factor-1 (Apaf-1) and active caspase-9 that results in the activation of caspase-359.
In the pre-antibiotic era when acute bacterial meningitis was described the mortality rate was from 90 to 100%. Although, nowadays, with the development of highly effective antibiotics, more precocity in diagnosis with precise diagnostic methods as immunological and molecular biology methods, supportive care in intensive care units the mortality doesn't decrease. The mortality of S. pneumoniae is (16-37%), N. meningitidis (5%), Haemophilus influenzae (3%). This maintenance of mortality is explained by inflammation in the subarachnoid space caused by the generation of bacterial cell wall components in CSF during treatment of meningitis with antibiotics2,60,61. Bacterial cell wall components stimulate the release of inflammatory cytokines in the CNS such as TNF-α, IL-1β, and prostaglandins.
Possible therapeutic approaches to decrease the harmful effects of TNF and/or IL-1-β are investigated in vitro and animal model and might include: a) Drugs or procedures to decrease their production, block their biologic activity or enhance removal from the circulation; b) Passive immunization with antibodies against TNF and IL-1, limitation is the BBB even during inflammation; c). Drugs that interfere with IL-1 induced arachidonic acid metabolites.
Corticosteroids are largely used as adjunctive therapy for acute bacterial meningitis since the 90 decade.
In addition to corticosteroids, several other adjunctive approaches may be useful (Table)60-67; although there is no proved clinical evidence for use of these therapies and some are only experimental. The use of bactericidal but nonbacteriolitic antibiotics to reduce endotoxin and other injurious substance release into CSF as rifampicin and daptomycin have been investigated in animal models of pneumococcal meningitis63. Thalidomide acts by blocking TNF release from microglia65. An enhanced lifespan of activated neutrophils in the CSF contributes to massive leukocyte accumulation and host-driven cytotoxicity. Roscovitine, a purine derivative that induces apoptosis in neutrophils, is studied in mouse models. TNF-α inhibitor decrease TNF-α levels and pleocytosis in CSF68, blocking IL-6 intravenously in a rat model of pneumococcal meningitis reduced also pleocytosis69 and TGF-β2 administrated intraperitoneally reduced the subarachnoid inflammation in rats with pneumococcal meningitis54. Intracisternal administration of the caspase-3 inhibitor reduced apoptosis in the dentate gyrus hippocampal70 and blocking of caspase-1 demonstrated lower levels of IL-1β71.
Corticosteroids are highly effective in reducing IL-1β production in vitro and in vivo. A meta-analysis that included all double-blinded, randomized, placebo-controlled trials since 2001 that evaluated dexamethasone in bacterial meningitis concluded that adjunctive dexamethasone in bacterial meningitis may reduce mortality and hearing loss for patients in developing countries. There may be no benefit for individuals in developing nations with a high prevalence of HIV infection66. The intravenous dose of dexamethasone proposed is 2 x 0.4 mg/kg/24h or 4 x 8-12mg/24h, during 48 to 96 hours. The first dose was delivered prior or concomitantly with the first dose of parenteral antibiotic67. In previous studies we verified dexamethasone treatment reverses cognitive impairment but increases brain oxidative stress in rats submitted to pneumococcal meningitis72. Adjunctive dexamethasone in bacterial meningitis reduces mortality and hearing loss for patients but still needs more evaluation.
Despite the significant advances in treatment, pneumococcal meningitis remains one of the most important infectious diseases of the CNS with high mortality and morbidity. Experimental animal model with its limitations continues to provide the understanding of this complex pathophysiology disease and propose new therapies adjunctive.
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Laboratório de Microbiologia Experimental Universidade do Extremo Sul Catarinense
Avenida Universitária, 1.105
88806-000 Criciúma SC, Brasil
Received 02 January 2012
Received in final form 26 January 2012
Accepted 12 February 2012
Support: This research was supported by grants from CNPq, FAPESC, UNESC, INCT-TM and L'Oréal-UNESCO Brazil Fellowship for Women in Science 2011.
Conflict of interest: There is no conflict of interest to declare.