Proton magnetic spectroscopy agreed better with magnetic resonance image to lateralization of epileptogenic zone than with surface electroencephalography

Espectroscopia de prótons de hidrogênio na investigação ambulatorial das epilepsias extra-temporais

Abstracts

Objective

To analyze the agreement rate of proton magnetic spectroscopy with magnetic resonance image (MRI) and surface electroence-phalography (EEG) in extratemporal neocortical epilepsies.

Methods

A cross-sectional study, type series of cases included 33 patients, age range 13–59 years old, of both gender, presenting structural alteration identified by MRI (75.8%) or by neurophysiologic techniques (72.7%). The variables were alterations of N-acetyl-aspartate/choline, N-acetyl-aspartate/creatine, choline/creatine, and N-acetyl-aspartate/cho-line+creatine coefficient of asymmetry.

Results

Agreement rates of lateralization by coefficient of asymmetry of NAA/Cho, NAA/Cr, Co/Cr, and NAA/Cho+Cr with MRI, independent of alteration of surface EEG, were equal to 93.3, 57.9, 15.4, and 93.3%, respectively, modifying to 100, 33.3, 0, and 100%, in 16 patients, with lateralization agreement of MRI and surface EEG.

Conclusion

Proton magnetic spectroscopy agreed better with MRI to lateralization of epileptogenic zone than with surface EEG.

extratemporal neocortical epilepsy; magnetic resonance spectroscopy; EEG

Objetivo

Analisar a taxa de concordância da espectroscopia de prótons de hidrogênio com imagem de ressonância magnética (IRM) e o eletrencefalograma (EEG) de superfície nas epilepsias neocorticais extratemporais.

Métodos

Estudo transversal, série de casos, incluiu 33 pacientes, com idade de 13 a 59 anos, de ambos os gêneros, apresentando alteração estrutural à IRM (75,8%) ou neurofisiológica à (72,7%). As variáveis estudadas foram as alterações dos coeficientes de assimetria de N-acetil-aspartato/colina, N-acetil-aspartato/crea-tina, Colina/Creatina e N-acetil-aspartato/colina+creatina.

Resultados

As taxas de concordância de lateralização dos coeficientes de assimetria de NAA/Co, NAA/Cr, Co/Cr e NAA/Co+Cr com a IRM, independentemente de alterações do EGG de superfície, passaram de 93,3, 57,9, 15,4, 93,3%, respectivamente, para 100, 33,3, zero, 100%, em 16 pacientes, mostrando concordância de lateralização entre IRM e EEG de superfície.

Conclusão

A espectroscopia de prótons de hidrogênio concordou melhor com a lateralização da zona epileptogênica pela IRM do que com o EEG de superfície.

epilepsias neocorticais extratemporais; espectroscopia por ressonância magnética; EEG

Amongst the epilepsies, the focal, symptomatic or prob-ably symptomatic, and neocortical necessarily require the localization and the lateralization of epileptogenic zone to establish diagnose and to institute clinical or surgical adequate treatment. Due to the characteristics of the extratemporal epilepsies, which make them more complex than the temporal epilepsies, they require more sensible and specific diagnostic methods. These methods include electro-encephalography (EEG), magnetic resonance image (MRI), and, more recently, the hydrogen proton spectroscopy.

The surface EEG presents low sensibility for extratemporal epilepsies, when compared with identical application for temporal epilepsies, although it is considered an essential method for diagnose, characterization, and localization of these epilepsies¹, independent of epileptogenic zone.

The MRI is recognized as the best noninvasive method to the structural evaluation of brain^{2 3}, and also the method of election for epilepsies refractive to drug treatment with surgical indication, because MRI has high sensibility and specificity for lesions like tumors, neuronal migration errors (specially dysplasia), hypoxic ischemic injuries, infections, metabolic errors, trauma, neurocutaneous diseases, vascular malformations, gliosis, etc.⁴-⁶.

The advent of surgery for the treatment of epilepsies demanded the investigation of new methods for therapeutic planning. Functional magnetic resonance⁴, positron emission tomography and single photon emission tomography⁵, diffusion tensor resonance, and tractography of fibers were developed⁷-⁹. Nevertheless, there is still a lacuna of diagnose and therapeutical follow-up of patients with neocortical extratemporal epilepsies, which makes the possibility of cure for these seizures difficult.

The magnetic resonance spectroscopy (MRS) and the MRI use the same physical principles, differing only in the way by which these data are processed and presented. MRS has graphic images, instead of anatomical images, where some brain metabolites, invisible on MRI, are identified¹⁰. The hydrogen proton spectroscopy is more used, due to the abundance of this atom in the organism, and also due to the fact that it provides a more intense signal¹¹, which permits the identification of the metabolites N-acetyl-aspartate (NAA), choline (Cho), creatine (Cr), amongst others^{10 12}.

For epileptic individuals, NAA may be reduced due to diffusion, neuronal lesion, or an increase of the energy consumption wasted on electrical discharges^{13 14}.

On epilepsies, the researchers have objectified to identify the relation between the metabolite alterations and the modifications on EEG, video EEG, and MRI, to establish diagnostic standards of localization and lateralization of epileptogenic zone. The studies involving normal persons concluded that brain asymmetries right-left type are not found, and the distribution patterns of NAA, Cho, and Cr metabolites are specific to each brain region¹³.

These findings pointed out the possibility that spectroscopy, in the future, may constitute an important exam within the arsenal of therapeutic, diagnose, and follow-up planning, and this has been the motivation for this research, which aims to analyze the efficacy of hydrogen proton magnetic spectroscopy for ambulatory evaluation of focal extratemporal epilepsies.

METHODS

The study has been a cross-sectional, type series of cases. The patient group consisted of persons, age range 13–59 years old, of both gender, with focal neocortical extratemporal epilepsy, diagnosed at the outpatient department from March to October 2006, presenting a unilateral lesion diagnosed by MRI or a lateralized interictal abnormality identified by surface EEG. The exclusion criteria were the presence of concomitant diagnose of other types of epilepsy, constant absence to ambulatory follow-up consultations, presence of diffuse lesion identified by MRI, undefined lateralization identified by EEG, and the refusal of the patient or his responsible to participate in the research.

Three centers participated in this research: The Epilepsy Ambulatory of Hospital da Restauração (Recife, Pernambuco, Brazil) where the patient group were diagnosed, followed, and submitted to EEG; the Multimagem Radiology Service (Recife, Pernambuco, Brazil), where MRI and 1H⁺MRS were performed; and the Radiology Service of Clinical Hospital at Universidade de São Paulo (São Paulo, Brazil), where data were processed and analyzed.

The sample constituted of 33 patients, with mean age equal to 25.18±11.39 years, varying from 13 to 59 years old, with a predominance of male gender (63.6%), less than four years of instruction (60.6%) and occupation as student (45.5%). For 33.3% of patients, epileptic seizures were focal secondary generalized, amongst which 75.8% were symptomatic. The nosologies of epilepsy more often were pre- or perinatal hypoxia (27.4%), head trauma (15.2%), and postsur-gical status (9.1%).

The variables were alterations in brain metabolite ratio NAA/Cho, NAA/Cr, and NAA/Cho+Cr, evaluated by pike metabolite area of 1H⁺MRS with long ET of 135 ms and multivoxel simultaneous acquisition; morphological alterations diagnosed by MRI (normal and with lesion); and neurophysi-ological alterations identified by EEG (normal and with interictal activity).

After a neurological anamnesis, the authors obtained data concerning patient's identification and submitted them to MRI with a 1.5 Tesla equipment, model Signa Infinity (General Electric Health Care, Milwaukee, WI, USA). The T1 and T2 image sequences of brain magnetic resonance were obtained, before contrast, within axial and coronal planes, with the patient in dorsal decubit at the exam table.

The parameters for spin-echo axial image acquisition on T1 were repetition time of 500 ms, echo time 14 ms, slice thickness 5 mm, slice interval 2.5 mm, matrix 256×192 and number of excitations (NEX) equal to 2. Axial images on T2, with fast spin-echo technique, were obtained with RT=4000 ms, ET=100 ms, ST=5mm and SI=2.5 mm, matrix=320×224 and NEX=2. The spoiled gradient-recalled echo (SPGR) volume technique has also been used with RT=25 ms, ET=4.2, FOV=24 cm, slice=0.5×0.7, matrix=192×192, and NEX=1.

To perform multivoxel 1H⁺MRS, a T2 axial image of brain was the reference. Within this image, one has identified voxel localization, guided by EEG or MRI, and selected the region for 1H⁺MRS, which has been compared with the contralateral one.

Multivoxel 1H⁺MRS was performed by point-resolved spec-troscopy technique (PRESS), with RT=1500 ms, ET=135 ms, voxel slice=10 mm, vision field=24 cm, phase codification=16×16, NEX=1, and anterior posterior frequency direction.

After multivoxel 1H⁺MRS acquisition, one has injected contrast with a volume correspondent to gadopentetate dimeglutamine (Magnograf^®) 0.2 mL per kg of the patient's body weight, intravenously, within approximately 30 s. The acquisition of postcontrast T1 images was obtained in sagittal, coronal, and axial planes and concluded the first phase of multivoxel 1H⁺MRS.

The second phase, also named data postprocessing, aimed to transform raw data into graphics. It has been performed at a work station Sun 60–Ultrasparc^®, at the Radiology Department of Clinics Hospital of Universidade de São Paulo, São Paulo, Brazil. Raw data were transferred from diskettes and compact disks to the work station, where they were converted with SA/GE^® (General Electric Health Care, Milwaukee, WI, USA) software.

The chosen voxel was localized at the white matter of the analyzed brain lobe, next to the lesion when localized by MRI, or even next to the brain cortex, when analyzed by EEG (Figure). In accordance to these criteria, to avoid voxels on peripheral areas that could be noisy or present diminished signal, two or three integers of pick areas of each brain metabolite (NAA, Cho, and Cr) were registered on a special protocol.

Figure.
Patient 20 – Complex tuberous sclerosis and epileptogenic zone lateralized to the right brain hemisphere by magnetic resonance image and 1H⁺ magnetic resonance spectroscopy. (A) Axial T₁ image, with hyperintense subependimary nodules; (B) Axial T₁ image, showing calcified cortical and subcortical tuber in right frontal lobe; (C) Axial T₂ image used to place voxel in frontal region. Spectroscopic graphic demonstrates a significant reduction in NAA/Cho, NAA/Cr, and NAA/Cho+Cr ratios on the right frontal lesion.

In the third phase, the analysis of multivoxel 1H⁺MRS consisted in the determination of metabolite means, to calculate metabolite indexes by NAA/Cr, NAA/Cho, NAA/Cho+Cr, and Cho/Cr ratios, followed by the determination of the respective coefficients of asymmetry (C_a ), according to formula¹⁵:

where:

x → analyzed metabolite ratio

R_left → value of metabolite ratio in the left brain hemisphere

R_right → value of metabolite ratio in the right brain hemisphere

The coefficients of asymmetry of patients were compared with those of the control group, by ANOVA test, followed by t-test for paired samples at a significance level of 0.05.

Surface EEG were performed at the Electroencephalog-raphy Department of Epilepsy and Neurophysiology Center, by 10-20 system, on scalp with 20 electrodes, of EMSA^® equipment, brainwave model, using a 20-min standard examination, and analysis with 15 mm/s speed and 100 µV amplitude. Activation methods were ocular opening and closure, photo stimulation, and hyperpnoea.

Data organization was performed within EPI-INFO software version 6.04d and statistical analysis with Statistical Package for Social Sciences, version 13.0.

To analyze the concordance of epileptic zone lateralization between complementary methods used in this research, we admitted that individuals without neurological diseases have no significant differences between metabolite concentration and its ratios in the right brain hemisphere compared with the left, for voxels within correlated areas, as well as there is a reduction of metabolite concentration and ratios in the brain hemisphere with epileptogenic foci.

Due to these assumptions and assuming the 95% confidence interval of control group as reference, a coefficient of asymmetry minor than the lower limit has indicated right lateralization, and, when major than the upper limit, left lateralization.

This research has been approved by the Ethics Committee on Research of Hospital da Restauração, under registration CAAE n°. 0086.0.102.840.05. All patients agreed to participate in this study by signing an Informed Free Consent Term after receiving explication about the objectives of the research and elucidation of possible doubts.

RESULTS

Table 1 expresses case to case, type, and anatomical lesion localization by MRI; interictal alteration and its projection in brain cortex diagnosed by surface EEG; as well as the inter-hemisphere coefficients of asymmetry.

Table 1.
Distribution of MRI and electroencephalography results and interhemispheric coefficients of asymmetry of 33 patients with neocortical extratemporal epilepsy – Recife, March/October 2006.

Reg	MRI		EEG		Ca NAA/Cho*	Ca NAA/Cr*	Ca NAA/Cho+Cr*	Ca Cho/Cr*
	Result	Lesion localization	Result	Localization
1	CNS primary neoplasia	Frontal R	Normal		0.2215	0.1034	-0.1188	0.1648
2	Gliosis	Frontal L	Normal		-0.0080	-0.2875	-0.2796	-0.1383
3	Normal		Spike wave and polispike wave	Frontal L	-0.3257	-0.2258	0.1018	-0.2651
4	Gliosis	Parietoccipital L	Sharp wave and slow wave	Parietoccipital L	-0.1230	0.3895	0.5064	0.1118
5	Gliosis	Frontoparietoccipital R	Teta regional rhythm	Frontoparietal R	0.0270	-0.2912	-0.3176	-0.1287
6	Gliosis	Frontoccipital L	Normal		0.0182	-0.2157	-0.2337	-0.1034
7	Gliosis	Parietoccipital L	Spike and spike wave	Parietoccipital L	-0.1861	0.2478	0.4290	0.0133
8	Gliosis	Frontal R	Spike wave	Frontal R	0.0133	-0.2894	-0.3024	-0.0696
9	Normal		Spike wave, sharp wave, and slow wave	Frontal R	0.1514	0.1991	0.0481	0.1716
10	Dysplasia	Frontal L	Normal		-0.0440	-0.2878	-0.2445	-0.1480
11	Gliosis	Frontal L	Spike wave, sharp wave, and slow wave	Frontal R	-0.0877	-0.2394	-0.1525	-0.1724
12	Normal		Spike wave, sharp wave, and slow wave	Parietoccipital L	-0.0674	0.1447	0.2116	0.0395
13	Gliosis	Frontal R	Spike and sharp wave	Frontal R	-0.0126	-0.1288	-0.1163	-0.0695
14	Polymicrogyria	Frontoccipital L	Normal		0.2481	-0.0176	-0.2654	0.1255
15	Normal		Sharp wave	Frontal R	0.1666	-0.4565	-0.6115	-0.2128
16	Gliosis	Frontal L	Spike and spike wave	Frontal L	-0.3081	0.0016	0.3097	-0.1296
17	Dysplasia	Parietoccipital L	Sharp wave and slow wave	Parietal L	-0.4363	-0.4612	-0.0263	-0.4482
18	Normal		Spike wave, sharp wave, and slow wave	Frontal R	-0.1583	-0.3734	-0.2183	-0.2684
19	Gliosis	Frontoparietal L	Spike wave	Frontoparietal L	-0.4201	0.3129	0.7096	-0.1079
20	Cortical tuber	Frontal R	Normal		0.2672	0.1787	-0.0896	0.2206
21	Neurocisticercosis	Frontal R	Normal		-0.0679	0.1756	0.2427	0.0476
22	Normal		Spike wave, sharp wave, and slow wave	Frontoparietal L	0.1424	-0.0714	-0.2133	0.0490
23	CNS venous malformation	Frontal R	Sharp wave and slow wave	Frontal L	0.2076	0.0417	-0.1662	0.1288
24	Normal		Sharp wave and slow wave	Frontal R	-0.5495	-0.6029	-0.0582	-0.5730
25	Normal		Sharp wave	Frontal R	0.0777	-0.0373	-0.1149	0.0239
26	Gliosis	Frontal L	Low amplitude	Frontal L	-0.0975	-0.3437	-0.2483	-0.2460
27	Gliosis	Frontoparietal R	Sharp wave and slow wave	Frontoparietal R	0.0978	-0.1858	-0.2824	-0.0480
28	Gliosis	Parietal R	Teta regional rhythm	Parietoccipital R	0.6415	-0.2930	-0.8925	0.1212
29	Gliosis	Frontoparietoccipital R	Delta regional rhythm	Frontal R	0.8909	0.2328	-0.6941	0.5807
30	Gliosis	Frontoparietoccipital L	Spike wave	Frontal L	-0.0824	0.1490	0.2307	0.0112
31	Neurocisticercosis	Frontal L	Normal		0.1067	0.0242	-0.0826	0.0637
32	Gliosis	Parietal L	Spike wave and sharp wave	Frontoparietal L	-0.2118	-0.3210	-0.1111	-0.2683
33	Gliosis	Frontal L	Normal		-0.4238	-0.2245	0.2041	-0.3158

• Negrite values were minor or major than the respective 95% confidence interval limits. MRI: magnetic resonance image; L: left; R: right; CNS: central nervous system.

Comparing the coefficients of asymmetry of NAA/Cho, NAA/Cr, NAA/Cho+Cr, and Cho/Cr ratios of 33 patients with the limits of a 95% confidence interval of control group, one has identified that 29 (87.9%) patients presented significant differences: 18 (54.5%) for NAA/Cho, 25 (75.7%) for NAA/Cr, 20 (60.6%) for NAA/Cho+Cr, and 16 (48.5%) for Cho/Cr.

Considering only 25 patients who presented lateralized lesion by MRI, 23 (92%) had a significant coefficient of asymmetry compared with the reference group, 15 (60.0%) with NAA/Cho or NAA/Cho+Cr ratios, 19 (76%) with NAA/Cr ratios, and 13 (52%) with Cho/Cr ratios.

Lateralization concordance rates between MRI and 1H⁺MRS were 93.3, 57.9, 93.3, and 15.4%, respectively, evaluated by NAA/Cho, NAA/Cr, NAA/Cho+Cr, and Cho/Cr ratios (Table 2).

Table 2.
Distribution of asymmetry coefficients of brain metabolite ratios evaluated by the area of spikes on spectroscopy graphic, according to lateralization determined by MRI and electroencephalography – Recife, March/October 2006.

Evaluation of lateralization concordance	Ca NAA/Cho n (%)	Ca NAA/Cr n (%)	Ca Cho/Cr n (%)	Ca NAA/Cho+Cr n (%)
Comparison of abnormal MRI with 1H+MRS
Patients with 1H+MRS altered	15 (60.0)	19 (76.0)	13 (52.0)	15 (60.0)
Concordance rate with 1H+MRS	14 (93.3)	11 (57.9)	2 (15.4)	14 (93.3)
Comparison of abnormal EEG with 1H+MRS
Patients with 1H+MRS altered	14 (58.3)	19 (79.2)	13 (54.2)	14 (58.3)
Concordance rate with 1H+MRS	11 (78.6)	6 (31.6)	–	8 (57.2)
Comparison of abnormal MRI and abnormal EEG with 1H+MRS
Patients with 1H+MRS altered	11 (68.8)	13 (81.3)	10 (62.5)	9 (56.3)
Concordance rate of EEG with 1H+MRS1	10 (91.0)	4 (30.8)	–	6 (66.7)
Concordance rate of MRI with 1H+MRS1	11 (100)	5 (38.5)	–	8 (88.9)
Concordance rate of EEG and MRI with 1H+MRS2	10/10 (100)	4/12 (33.3)	–	6/6 (100)
Comparison of abnormal MRI and normal EEG with 1H+MRS
Patients with 1H+MRS altered	4 (44.4)	6 (66.7)	3 (33.3)	6 (66.7)
Concordance rate of MRI with 1H+MRS1	3 (75)	6 (100)	2 (66.7)	6 (100)
Comparison of normal MRI and abnormal EEG with 1H+MRS
Patients with 1H+MRS altered3	3 (37.5)	6 (75)	3 (37.5)	5 (62.5)
Concordance rate of EEG with 1H+MRS1	1 (33.3)	2 (33.3)	–	2 (40)

• ¹
  Percentages based on total of patients with abnormal differences of asymmetry coefficient;
• ²
  Percentages based on a total of patients with concordant later-alization between EEG and MRI;
• ³
  Percentages based on eight patients with abnormalities restricted to EEG; MRI: magnetic resonance image; MRS: magnetic resonance spectroscopy.

With respect to 24 patients who presented lateralization by interictal activity by EEG, 21 (87.5%) showed a significant coefficient of asymmetry when compared with the reference group, 14 (58.3%) with NAA/Cho and NAA/Cho+Cr ratios, 19 (79.2%) with NAA/Cr ratios, and 13 (54.2%) with Cho/Cr ratios.

Lateralization concordance rates between EEG and 1H⁺MRS were equal to 78.6, 31.6, and 57.2%, related to NAA/Cho, NAA/Cr, and NAA/Cho+Cr ratios, respectively. There was no concordance between EEG and Cho/Cr ratios (Table 2).

Concerning 16 patients who presented lateralization of interictal activity identified by EEG and an anatomical lesion by MRI, 15 (93.8%) showed a significant coefficient of asymmetry when compared with the reference group, 11 (68.8%) with NAA/Cho ratios, 13 (81.3%) with NAA/Cr ratios, 9 (56.3%) with NAA/Cho+Cr ratios, and 10 (62.5%) with Cho/Cr ratios. Lateralization concordance rates between EEG and 1H⁺MRS were equal to 91, 30.8, and 66.7%, when evaluated by NAA/Cho, NAA/Cr, and NAA/Cho+Cr, respectively, with no concordance to Cho/Cr ratios, while concordance rates corresponding to MRI were equal to 100%, 38.5%, and 88.9%, respectively, and again with no concordance to Cho/Cr ratios (Table 2).

The comparison of epileptogenic zone lateralization, identified by the three methods, showed a concordance rate equal to 100%, 33.3%, and 100%, respectively for NAA/Cho, NAA/Cr, and NAA/Cho+Cr, reaching 0 for Cho/Cr ratios (Table 2).

Among nine patients with lesion lateralization on MRI associated with a normal EEG, eight (88.9%) had a significant coefficient of asymmetry when compared with the reference group, three (44.4%) with NAA/Cho ratios, six (66.7%) with NAA/Cr ratios, three (33.3%) with Cho/Cr ratios, and six (66.7%) with NAA/Cho+Cr ratios. Lateralization concordance rates between MRI and 1H⁺MRS, associated with normal EEG, equaled 75, 100, 66.7, and 100%, respectively evaluated by NAA/Cho, NAA/Cr, Cho/Cr, and NAA/Cho+Cr (Table 2).

When eight patients, with interictal abnormality lateralized by EEG and associated with normal MRI, were considered, six (75%) presented a significant difference of coefficient of asymmetry compared with the reference group, three (37.5%) with NAA/Cho ratios, six (75%) with NAA/Cr ratios, three (37.5%) with Cho/Cr ratios, and five (62.5%) with NAA/ Cho+Cr ratios. Lateralization concordance rates between EEG and 1H⁺MRS, associated with normal MRI, were equal to 33.3% for NAA/Cho and NAA/Cr ratios, 40% for NAA/Cho+Cr ratios, and 0 for Cho/Cr ratios (Table 2).

DISCUSSION

The localization and lateralization of the epileptogenic zone received special attention after the advent of surgical treatment, because the determination of this zone permitted its resection, which can cure seizures, when successful, or at least can adequately control them with small doses of antiepileptic drugs¹⁶.

Among epilepsies, the neocortical present high level of diagnose difficulty due to the great number of brain intra-and interhemisphere network connections, which generate diagnose doubts concerning neurophysiology, functional neuroradiology, and neuroimaging¹⁷.

These challenges motivated the research methods able to refine the localization and lateralization of epileptogenic zone, including routine EEG, long-term video-EEG, MRI, PET, SPECT, and hydrogen proton spectroscopy¹⁸.

While routine EEG investigate types and localization of electric brain abnormalities, the MRI shows encephalic structural lesions, and the MRS determines the disturbances of brain metabolites, such as NAA, Cho, and Cr^{15 19}-²¹.

Based on the aspects of brain metabolism here pointed out, we tried to explain the results of the asymmetry coefficient reduction of NAA/Cho, NAA/Cr, NAA/Cho+Cr, and Cho/Cr in patients with neocortical extratemporal epilepsy.

In epilepsy, there is a reduction of neuron population derived from the physical and chemical aggression, which determines neuronal damage with reduction of NAA mito-chondrial synthesis, represented by diminishing of this metabolite concentration, which is biomarker of neuron quantity and viability²².

Another possible mechanism implied in the reduction of NAA is based on the comparison of generalized and occipital epilepsies. The first ones show increase of glutamine-gluta-mate and GABA, while on the second ones this increase is restricted just to GABA. Identifying many interictal epilep-togenic discharges, we can admit the hypothesis of a great demand of glutamine-glutamate (excitatory neurotrans-mitters), and as a compensatory mechanism, the increase of GABA synthesis to inhibit the process, determining the decrease NAA²³.

The asymmetry coefficient of NAA/Cho, NAA/Cr, Cho/Cr, and NAA/Cho+Cr between the hemispheres in neocortical extratemporal epilepsies may be explained by a metabolic abnormality characterized by the reduction of NAA in dysfunctional side, secondary to an augmentation of energy consumption due to a high metabolic activity necessary to generate abnormal electrical activity in epileptogenic zone. This change of metabolic activity promoted an increase in creatine synthesis, determining the reduction of NAA/Cr ratio²⁴.

One can still admit that epilepsy, at cell level, consists of abnormal functioning derived by intercellular hyperexcitability associated with hypocellularity. This implies on admitting that brain tissue of epileptic patient presents cellular reduction expressed by the diminution of NAA, metabolic increase identified by creatine augmentation, and a great cellular turnover characterized by the choline increasing¹⁵.

The importance of this knowledge is to aid in the localization of epileptogenic zone on the orientation of surgical treatment. Therefore, when there is concordance of lateralization between EEG and MRI, the diagnose of this zone may be performed without another method, restricting MRS use to help anatomical localization in cases of discordance or doubt²⁵. These affirmatives have been confirmed in this research by the lateralization concordance ratios of asymmetry coefficients equal to 100% for NAA/Cho and NAA/Cho+Cr and 33.3% for NAA/Cr, for patients with a concordant side of abnormalities in MRI and EEG.

Within clinical practice, when the results of MRI and EEG are inconclusive about the identification of epileptogenic zone, it requires the use of invasive methods such as profound electrodes or subdural grid and stripes — procedures of high cost. In such cases, the association of MRS may help surgical planning²⁶.

The MRS with simultaneous multivoxel acquisition and long ET of 135 ms showed efficacy on lateralization of epi-leptogenic zone compared to MRI, which is a high sensibility and specificity method for patients with localized brain structural lesion, because the structural lesion is within the epileptogenic area, in most cases, independent of EEG later-alization. This may constitute one more resource to improve the confidence on localization and lateralization of brain area to be resected²⁷.

The lateralization of epileptogenic zone compared with the lateralization of epileptogenic discharges, localized and lateralized by EEG, is less effective, because routine EEG is a method less sensible and specific to the determination of epileptogenic zone for neocortical extratemporal epilepsies²¹.

In this research, there was a great reduction in lateralization concordance ratios identified by NAA/Cho, NAA/Cr, and NAA/Cho+Cr asymmetry coefficients, when comparing lateralization and localization performed exclusively by routine EEG, independent of MRI findings, with altered EEG and normal MRI (78.6 to 33.3%, 31.6 to 33.3%, and 57.2 to 40%, respectively). These differences suggested that the zones of interictal discharges may not coincide with epileptogenic zones¹⁵.

Additionally, when comparing patients with lateralization and localization performed exclusively by MRI abnormality, independent of EEG findings, with those with abnormal MRI and normal EEG, we identified the reduction of concordance ratios of NAA/Cho asymmetry coefficients (93.3 to 75%), while for NAA/Cr and NAA/Cho+Cr asymmetry coefficients there was significant increase (57.9 to 100% and 93.3 to 100%, respectively).

We must exercise caution to the interpretation of our findings, related to EEG sensibility and specificity, which can be a limitation of this research. Although the ictal EEG is admitted as gold standard for the localization and lateralization of epileptogenic zone, in some cases its accuracy does not provide the best conditions for guiding epileptic surgery. This research, as others in literature^{10 15 18 24 25}, point out MRS as another option to contribute for localization and lateralization of epileptogenic zone in these cases.

Resuming, in this research, on one hand, we identified a low concordance between focal interictal EEG findings and MRS for the lateralization of epileptogenic zone. On the other hand, there was high coincidence of lateralization when we compared a localized lesion MRI with MRS, identified by the difference of NAA/Cho+Cr asymmetry coefficients between brain hemispheres.

According to these findings, we admitted that the asymmetry coefficients of brain metabolic ratios, which differ significantly between abnormal and normal hemispheres, demonstrate the interrelations between distinct metabolic pathways altered in epilepsies.

We concluded that MRS with simultaneous multivoxel and long ET 135 ms can be a complementary method for lateralization of epileptogenic zones in neocortical extratemporal epilepsies with structural lesion identified by MRI. It represents a great perspective of equal success on the lateralization of epileptogenic zones in the absence of MRI findings, but researches will be required for comparing MRS to invasive video EEG and stereo EEG (deep electrodes).

References

• ¹
  Leach JP, Stephen LJ, Salveta C, Brodie MJ. Which electroencepha-lography (EEG) for epilepsy? The relative usefulness of different EEG protocols in patients with possible epilepsy. J Neurol Neurosurg Psychiatry 2006;77:1040-1042.
• ²
  Nóvak EM, Terabe F, Nasimoto AL, et al. Correlação entre hipótese diagnóstica e laudo de tomografia axial computadorizada craniana. Arq Neuropsiquiatr 2001;59:761-767.
• ³
  Wright NB. Imaging in epilepsy: a paediatric perspective. Br J Radiol 2001;74:575-589.
• ⁴
  Bonilha L, Montenegro MA, Cendes F, Li LM. The role of neuroimaging in the investigation of patients with single seizures, febrile seizures, or refractory partial seizures. Med Sci Monit 2004;10:RA40-RA46.
• ⁵
  Connor SEJ, Jarosz JM. Magnetic resonance imaging of patients with epilepsy. Clin Radiol 2001;56:787-801.
• ⁶
  Duncan JS, Sander JW, Sisodiya SM, Walker MC. Adult epilepsy. Lancet 2006;367:1087-1100.
• ⁷
  Concha L, Gross W, Wheatley BM, Beaulieu C. Diffusion tensor imaging of time-dependent axonal and myelin degradation after corpus callosotomy in epilepsy patients. NeuroImage 2006;32:1090-1099.
• ⁸
  Lee SK, Mori S, Kim DJ, et al. Diffusion tensor MRI and fiber tractography of cerebellar atrophy in phenytoin users. Epilepsia 2003;44:1536-1540.
• ⁹
  Isik U, Dincer A, Özek MM. Surgical treatment of polymicrogyria with advanced radiologic and neurophysiologic techniques. Childs Nerv Syst 2007;23:443-448.
• ¹⁰
  Danielsen ER, Ross B. Magnetic resonance spectroscopy diagnosis of surgical diseases. USA: Marcel Dekker, 1999:327.
• ¹¹
  Ross BD. A biochemistry primer for neuroradiologists. In: Advanced imaging symposium: preparing the neuroradiologist for the new millennium. Annual Meeting of the American Society of Neuroradiology, Atlanta 2000:13-27.
• ¹²
  Henry RG, Vigneron DB, Fischbein NJ, et al. Comparison of relative cerebral blood volume and proton spectroscopy in patients with treated gliomas. Am J Neuroradiol 2000;21:357-366.
• ¹³
  Bonavita S, Di Salle F, Tedeschi G. Proton MRS in neurological disorders. Eur J Radiol 1999;30:125-131.
• ¹⁴
  Moffett JR, Ross B, Arun P, Madhavarao CN, Namboodiri AMA. N-Acetylaspartate in the CNS: from neurodiagnostics to neurobiology. Prog Neurobiol 2007;81:89-131.
• ¹⁵
  Krsek P, Hajik M, Dezortova M, et al. H MR spectroscopic imaging in patients with MRI-negative extratemporal epilepsy: correlation with ictal onset zone and histopathology. Eur Radiol 2007;8:2126-2135.
• ¹⁶
  Palmini A. The concept of the epileptogenic zone: a modern look at Penfield and Jasper's views on the role of interictal spikes. Epileptic Disord 2006;8(Suppl 2):S10-S15.
• ¹⁷
  Stanley JA, Cendes F, Dubeau F, Andermann F, Arnold DL. Proton magnetic resonance spectroscopic imaging in patients with extratemporal epilepsy. Epilepsia 1998;39:267-273.
• ¹⁸
  Leite RAA, Otaduy MCG, Silva GEG, Ferreira MLB, Aragão MFV. Diagnostic methods for extra-temporal neocortical focal epilepsies. Arq Neuropsiquiatr 2010;68:119-126.
• ¹⁹
  Blume W. Diagnosis and management of epilepsy. CMAJ 2003;168:441-448.
• ²⁰
  Janszky J. Diagnosis of epilepsy. Ideggyogy Sz 2004;57:157-163.
• ²¹
  Verma A, Radtke R. EEG of partial seizures. J Clin Neurophysiol 2006;23:333-339.
• ²²
  Briellmann RS, Pell GS, Wellard RM, et al. MR imaging of epilepsy: state of the art at 1.5 T and potential of 3 T. Epileptic Dis 2003;5:3-20.
• ²³
  Simister RJ, Mc Lean MA, Barker GJ, Duncan JS. A proton magnetic resonance spectroscopy study of metabolites in the occipital lobes in epilepsy. Epilepsia 2003;44:550-558.
• ²⁴
  Lundbom N, Gaily E, Vuori K, et al. Proton spectroscopic imaging shows abnormalities in glial and neuronal cell pools in frontal lobe epilepsy. Epilepsia 2001;42:1507-1514.
• ²⁵
  Willmann O, Wennberg R, May T, Woermann FG, Pohlmann-Eden B. The hole of 1H magnetic resonance spectroscopy in pre-operative evaluation for epilepsy surgery. A meta-analysis. Epilepsy Res 2006;71:149-158.
• ²⁶
  Zumsteg D, Wieser HG. Presurgical evaluation: Current role of invasive EEG. Epilepsia 2000;41(Suppl 3):S55-S60.
• ²⁷
  Centeno R, Yacubian EM, Sakamoto AC, Ferraz AFP, Carrete Jr. H, Cavalheiro S. Pre-surgical evaluation and surgical treatment in children with extratemporal epilepsy. Childs Nerv Syst 2006; 22:945-959.

Publication Dates

• Publication in this collection
  Sept 2013

History

• Received
  2 Sept 2012
• Received
  23 Apr 2013
• Accepted
  30 Apr 2013