Propagação de orquídeas por sementes

Franco, Coarací M.

doi:10.1590/S0006-87051942000700001

Resumo

Knudson's asymbiotic method and medium for germination of orchid seeds were used with success. Heating this medium at 100°C twice for 15 minutes with an interval of 24 hours was found to be better than to sterilize just once at 120°C. This procedure avoids the hydrolysis of the agar. The poor quality of the glass from which some test tubes were made raised the pH tabove 5.9 and this was apparently the cause of poor developmen of the seedlings. Also some toxic elements given off by the glass diffusing thru the medium might havo impaired somewhat the results obtained in the experiment.

SUMMARY

Knudson's asymbiotic method and medium for germination of orchid seeds were used with success.

Heating this medium at 100°C twice for 15 minutes with an interval of 24 hours was found to be better than to sterilize just once at 120°C. This procedure avoids the hydrolysis of the agar.

The poor quality of the glass from which some test tubes were made raised the pH tabove 5.9 and this was apparently the cause of poor developmen of the seedlings.

Also some toxic elements given off by the glass diffusing thru the medium might havo impaired somewhat the results obtained in the experiment.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

LITERATURA CITADA

1. Bernard, N. L'evolution dans la symbiose. L'Orchidées et leur champignons com- mensaux. Ann. Sci. Nat. Bot. 9: 1-96, 1909.
2. Burgeff, H. Em Die Wurzelpilze der Orchideen, ihre Kultur und ihr Leben in der Pflanze, Jena, 1909.
3. Hoehne, F. C. Em Album de Orquidáceas Brasileiras e o Orquidário do Estado de São Paulo. Publ. Secr. Agr. Estado de São Paulo, pág. 1-264, il., 1930.
4. Knudson, L. Nonsymbiotic germination of orchid seeds. Bot. Gaz. 73: 1-25, 1922.
5. Knudson, L. Further observations on nonsymbiotic germination of orchid seeds. Botl. Gaz. 77: 212-219, 1924.
6. Knudson, L. Physiological study of the symbiotic germination o orchid seeds. Bot. Gaz. 79:345-379, 1925.
7. Knudson, L. Flower production by orchid grown non-symbiotically, Bot. Gaz 89; 192-299; Ilustr. 1930.
8. White, E. A. Em American Orchid Culture. 2.Ş edição, pág. 1-256, il., New York, De La Mare Co., 1939.
9. Wilson, J. K. Calcium hypochlorite as a seed sterilizer. Amer. Jour. Bot. 2: 420-427, 1915.