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Bragantia

Print version ISSN 0006-8705

Bragantia vol.24 no.unico Campinas  1965

http://dx.doi.org/10.1590/S0006-87051965000100019 

Ocorrência de vírus esférico causando faixa amarela das nervuras da couve em São Paulo

 

Occurrence of yellow vein banding of cole induced by a spherical virus

 

 

E. W. Kitajima; A. R. Oliveira; A. S. Costa

Engenheiros-agrônomos, Seção de Virologia, Instituto Agronômico

 

 


RESUMO

Partículas esferoidais de 50-60mμ de diâmetro foram encontradas em preparações semipurificadas e em preparações rápidas de plantas de couve infetadas com um vírus que induz faixa amarela das nervuras (VFANC). Essas partículas têm um capsídeo aparentemente icosaedral, constituído de 92 capsômeros de 50-60 A de diâmetro. Quando tratadas com acetato de uranila, revelaram a existência de uma zona central, que se impregna fortemente com êste corante, possìvelmente constituída de nucleoproteína. Tais partículas não foram encontradas em plantas sadias, mas foram nelas detectadas, após serem inoculadas por união de tecidos, por meios mecânicos ou por afídeos com VFANC.
Partículas similares foram encontradas em secções ultra-finas de tecido foliar de couve infetado com o VFANC, porém apresentavam um diâmetro ligeiramente menor, da ordem de 35-45m
μ. Invariàvelmente, essas partículas foram encontradas em meio às inclusões citoplasmáticas de forma e dimensões variadas, constituídas de uma massa amorfa, granular e densa.
Tais inclusões puderam também ser observadas em secções mais espêssas e montadas para microscopia óptica.
Possìvelmente o VFANC pertence ao grupo do vírus do mosaico da couve-flôr, dada a semelhança na morfologia das partículas e nas moléstias induzidas.


SUMMARY

Spherical particles, 50-60 mμ, in diameter were found in partially purified preparalions, and also in quick preparations made from plants infected with a virus inducing yellow vein banding in cole (Brassica oleracea var. acephala) . These particles apparently had an icosahedral capsid composed by 92 capsomeres, each with 50-60 A in diameter. Uranyl acetate treatment of these particles revealed a central zone, about 25-30 mμ in diameter, which bound strongly with the stain, indicating that this part of the particle is nucleoprotein. Such particles were absent in healthy plants, but they were detected after these plants were inoculated by tissue union, mechanically, or by aphids with the cole yellow vein banding virus.
Similar particles were found in ultrathin leaf sections from cole infected by yellow vein banding virus, invariably embedded in a cytoplasmic inclusion body, but their diameters were slightly smaller (35-45 m
μ). These inclusions, with variable dimensions and shape, were composed by an amorphous, dense and granular matrix, and were detectable even under light microscope level in thicker sections.
It is likely that the above described virus is related to the cauliflower mosaic virus, due to the similarities in particle morphology and induced diseases.


 

 

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Recebido para publicação em 25 de março de 1965.

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