Tolerância de Bradyrhizobium sp. de mimosoideae à acidez em meio de cultura

Ribeiro Júnior, Walter Quadros; Franco, Avílio Antonio; Lopes, Eli Sidney

doi:10.1590/S0006-87051988000200017

Resumos

Foram realizados testes em meio de cultivo acidificado para avaliar a tolerância de 59 estirpes de Bradyrhizobium sp. isolados de Mimosoideae. As culturas, por via de regra, apresentaram crescimento rápido e alcalinização do meio. Das estirpes testadas, dez apresentaram crescimento em meio com valor de pH 4,6 (três, crescimento rápido; um, médio e seis, lento). Destas, oito não induziram alteração visual na cor do indicador bromotimol-azul incluído no meio. A estirpe SMS-513, uma entre essas oito, promoveu acidificação no meio com valor de pH 6,2, sendo considerada tolerante à acidez. Algumas estirpes cresceram em meio de cultura acidificado, somente com alta concentração inicial de células.

rizóbios tolerantes à acidez; Mimosoideae; Bradyrhizobium sp

Fifty-nine Bradyrhizobium sp. strains isolated from Mimosoideae subfamily of Leguminosae were tested on acidified agar medium. Most strains were found to be fast growing and alcalinized the medium. Ten strains grew on pH 4.6; out of them, three were fast growing, six were slow growing and one was intermediate. Eight of the tested strains did not induce visual changes in the bromothymol-blue indicator. The strain SMS-513 acidified the medium with pH 6.2, and was considered acid tolerant.

acid tolerant rhizobia; Mimosoideae; Bradyrhizobium sp

VII. MICROBIOLOGIA DO SOLO

NOTA

Tolerância de Bradyrhizobium sp. de mimosoideae à acidez em meio de cultura

Tolerance of mimosoideae Bradyrhizobium sp. strains to acidity in culture media

Walter Quadros Ribeiro JúniorI, ^* * Com bolsa de pesquisa do CNPq. ; Avílio Antonio Franco^II; Eli Sidney LopesIII, ^* * Com bolsa de pesquisa do CNPq.

^IPesquisador da EMGOPA, Rua 58, n9 94, Centro, 74000 Goiás

^IIPesquisador da EMBRAPA, UAPNPBS, km 47, 23851 Seropédica, RJ

^IIISeção de Microbiologia do Solo, Instituto Agronômico (IAC), Caixa Postal 28, 13001 Campinas (SP)

RESUMO

Foram realizados testes em meio de cultivo acidificado para avaliar a tolerância de 59 estirpes de Bradyrhizobium sp. isolados de Mimosoideae. As culturas, por via de regra, apresentaram crescimento rápido e alcalinização do meio. Das estirpes testadas, dez apresentaram crescimento em meio com valor de pH 4,6 (três, crescimento rápido; um, médio e seis, lento). Destas, oito não induziram alteração visual na cor do indicador bromotimol-azul incluído no meio. A estirpe SMS-513, uma entre essas oito, promoveu acidificação no meio com valor de pH 6,2, sendo considerada tolerante à acidez. Algumas estirpes cresceram em meio de cultura acidificado, somente com alta concentração inicial de células.

Termos de indexação: rizóbios tolerantes à acidez; Mimosoideae; Bradyrhizobium sp.

SUMMARY

Fifty-nine Bradyrhizobium sp. strains isolated from Mimosoideae subfamily of Leguminosae were tested on acidified agar medium. Most strains were found to be fast growing and alcalinized the medium. Ten strains grew on pH 4.6; out of them, three were fast growing, six were slow growing and one was intermediate. Eight of the tested strains did not induce visual changes in the bromothymol-blue indicator. The strain SMS-513 acidified the medium with pH 6.2, and was considered acid tolerant.

Index terms: acid tolerant rhizobia, Mimosoideae, Bradyrhizobium sp.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Trabalho recebido para publicação em 7 de outubro de 1986 e aceito em 6 de setembro de 1988.

ANDREW, C.S. & JOHNSON, A.D. Effect of calcium, pH and nitrogen on the growth and chemical composition of some tropical and temperate pasture legumes. 1 Nodulation and growth. Australian Journal Agricultural Research, Melbourne, 27:611-623, 1976.
AYANABA, A.; ASANUMA, A. & MUNNS, D.M. An agar plate method for rapid screening of Rhizobium for tolerance to aluminum. Soil Science Society of the American Journal, Madison, 47:256-258, 1983.
BROSE, E. Seleçăo de estirpes de Rhizobium trifolii, tolerantes a baixo pH, P e alto alumínio para trevo branco. In: REUNIÃO LATINO-AMERICANA SOBRE RHIZOBIUM, 12., Campinas, 1984. Resumos. p.58.
COOPER, J.E. Acid production, acid tolerance and growth rate of Rhizobia in laboratory media. Soil Biological and Biochemistry, Elvisford, 14:127-131, 1982.
DATE, R.A. & HALLIDAY, J. Selecting Rhizobium for acid infertile soils of the tropics. Nature, London, 277:62-64, 1978.
FRANCO, A.A. & MUNNS, D.M. Acidity and aluminum restraints on nodulation, nitrogen fixation and growth of Phaseolus vulgaris in solution culture. Soil Science Society of the American Journal, Madison, 46:296-301, 1982.
GIBSON, D.I.; HAYES, P. & LAIDLAW, A.S. The influence of phosphate and lime on the growth and nitrogen fixation of Lotus uliginosus and Trifolium repens under greenhouse conditions. Journal of the British Grassland Society, Oxford, 30(4):295-301, 1975.
GRAHAM, P.M.; VITERI, F.M.; VARGAS, A.T. & PALACIOS, A. Variation in acid soil tolerance among strains of Rhizobium phaseoli. Field Crops Research, Amsterdan, 5:121-128,1982.
KEYSER, M.M. & MUNNS, D.N. Tolerance of rhizobia to acidity aluminum and phosphate. Soil Society of the American Journal, Madison, 43:519-523, 1979.
LIE, T.A. Symbiotic N fixation under stress conditions. Plant and Soil, The Hague, 127-177,1971. (nş especial)
MUNNS, D.M. Soil acidity and nodulation. In: ANDREW, C.S. & KAMPRATH, E.J., ed. Mineral nutrition of legumes in tropical and subtropical soils. Austrália, CSIRO, 1978. p.264-274.
PARKER, C.A. The significance of acid and alkali production by Rhizobium on laboratory media. In: LEGUME NODULATION CONFERENCE, 4., Canberra, 1971. Proceedings. Melbourne, CSIRO, 1971. 3p. (paper number eleven)
RIBEIRO JÚNIOR, W.Q.; FRANCO, A.A. & LOPES, E.S. Eficiência e competitividade de estirpes de Bradyrhizobium spp., para Enterolobium contortisiliquum, em latossolo ácido. Revista Brasileira de Ciência do Solo, Campinas, 10:219-225, 1986.
^__________; LOPES, E.S. & FRANCO, A.A. Eficiência de estirpes de Bradyrhizobium spp. para quatro leguminosas arbóreas e competitividade das estirpes em Albizia lebbek em latossolo ácido. Revista Brasileira de Ciência do Solo, Campinas, 11:275-282, 1987.
RICE, W.A.; PENNEY, D.C. & NYBERG, M. Effects of soil acidity on rhizobia numbers nodulation and nitrogen fixation by alfalfa and red clover. Canadian Journal of Soil Science, Ottawa, 57:197-203, 1977.
SILVA, G.G. & FRANCO, A.A. Seleção de estirpes de Rhizobium sp. de leguminosas florestais, em meio de cultura, tolerantes à acidez e à toxidez do alumínio. Pesquisa Agropecuária Brasileira, Brasília, 19:169-174, 1984.
SPAIN, J.M.; FRANCIS, C.A.; HOWELER, R.M. & CALVO, F. Differential species and varietal tolerance to soil acidity in tropical crops and pastures. In: BORHEMIZZA, E. & ALVARADO, A., eds. Soil Management in Tropical America. Cali, CIAT, 1975. p.308-347.

*

Com bolsa de pesquisa do CNPq.

Datas de Publicação

Publicação nesta coleção
27 Nov 2007
Data do Fascículo
1988

Histórico

Aceito
06 Set 1988
Recebido
07 Out 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] ANDREW, C.S. & JOHNSON, A.D. Effect of calcium, pH and nitrogen on the growth and chemical composition of some tropical and temperate pasture legumes. 1 Nodulation and growth. Australian Journal Agricultural Research, Melbourne, 27:611-623, 1976.

[2] AYANABA, A.; ASANUMA, A. & MUNNS, D.M. An agar plate method for rapid screening of Rhizobium for tolerance to aluminum. Soil Science Society of the American Journal, Madison, 47:256-258, 1983.

[3] BROSE, E. Seleçăo de estirpes de Rhizobium trifolii, tolerantes a baixo pH, P e alto alumínio para trevo branco. In: REUNIÃO LATINO-AMERICANA SOBRE RHIZOBIUM, 12., Campinas, 1984. Resumos. p.58.

[4] COOPER, J.E. Acid production, acid tolerance and growth rate of Rhizobia in laboratory media. Soil Biological and Biochemistry, Elvisford, 14:127-131, 1982.

[5] DATE, R.A. & HALLIDAY, J. Selecting Rhizobium for acid infertile soils of the tropics. Nature, London, 277:62-64, 1978.

[6] FRANCO, A.A. & MUNNS, D.M. Acidity and aluminum restraints on nodulation, nitrogen fixation and growth of Phaseolus vulgaris in solution culture. Soil Science Society of the American Journal, Madison, 46:296-301, 1982.

[7] GIBSON, D.I.; HAYES, P. & LAIDLAW, A.S. The influence of phosphate and lime on the growth and nitrogen fixation of Lotus uliginosus and Trifolium repens under greenhouse conditions. Journal of the British Grassland Society, Oxford, 30(4):295-301, 1975.

[8] GRAHAM, P.M.; VITERI, F.M.; VARGAS, A.T. & PALACIOS, A. Variation in acid soil tolerance among strains of Rhizobium phaseoli. Field Crops Research, Amsterdan, 5:121-128,1982.

[9] KEYSER, M.M. & MUNNS, D.N. Tolerance of rhizobia to acidity aluminum and phosphate. Soil Society of the American Journal, Madison, 43:519-523, 1979.

[10] LIE, T.A. Symbiotic N fixation under stress conditions. Plant and Soil, The Hague, 127-177,1971. (nş especial)

[11] MUNNS, D.M. Soil acidity and nodulation. In: ANDREW, C.S. & KAMPRATH, E.J., ed. Mineral nutrition of legumes in tropical and subtropical soils. Austrália, CSIRO, 1978. p.264-274.

[12] PARKER, C.A. The significance of acid and alkali production by Rhizobium on laboratory media. In: LEGUME NODULATION CONFERENCE, 4., Canberra, 1971. Proceedings. Melbourne, CSIRO, 1971. 3p. (paper number eleven)

[13] RIBEIRO JÚNIOR, W.Q.; FRANCO, A.A. & LOPES, E.S. Eficiência e competitividade de estirpes de Bradyrhizobium spp., para Enterolobium contortisiliquum, em latossolo ácido. Revista Brasileira de Ciência do Solo, Campinas, 10:219-225, 1986.

[14] ^__________; LOPES, E.S. & FRANCO, A.A. Eficiência de estirpes de Bradyrhizobium spp. para quatro leguminosas arbóreas e competitividade das estirpes em Albizia lebbek em latossolo ácido. Revista Brasileira de Ciência do Solo, Campinas, 11:275-282, 1987.

[15] RICE, W.A.; PENNEY, D.C. & NYBERG, M. Effects of soil acidity on rhizobia numbers nodulation and nitrogen fixation by alfalfa and red clover. Canadian Journal of Soil Science, Ottawa, 57:197-203, 1977.

[16] SILVA, G.G. & FRANCO, A.A. Seleção de estirpes de Rhizobium sp. de leguminosas florestais, em meio de cultura, tolerantes à acidez e à toxidez do alumínio. Pesquisa Agropecuária Brasileira, Brasília, 19:169-174, 1984.

[17] SPAIN, J.M.; FRANCIS, C.A.; HOWELER, R.M. & CALVO, F. Differential species and varietal tolerance to soil acidity in tropical crops and pastures. In: BORHEMIZZA, E. & ALVARADO, A., eds. Soil Management in Tropical America. Cali, CIAT, 1975. p.308-347.

Brasil

Brasil

Tolerância de Bradyrhizobium sp. de mimosoideae à acidez em meio de cultura

Tolerance of mimosoideae Bradyrhizobium sp. strains to acidity in culture media

Resumos

Datas de Publicação

Histórico