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Bragantia

Print version ISSN 0006-8705On-line version ISSN 1678-4499

Bragantia vol.48 no.2 Campinas  1989

http://dx.doi.org/10.1590/S0006-87051989000200003 

II. GENÉTICA E MELHORAMENTOS DE PLANTAS

 

Possibilidade de seleção recorrente para aumento do teor de óleo em soja com a utilização da macho-esterilidade genética e da espectroscopia de ressonância nuclear magnética1

 

The possibility of recurrent selection to increase the oil content in soybeans using genetic male sterility and NMR spectroscopy

 

 

Manoel Albino Coelho de MirandaI, 2; João Paulo Feijão TeixeiraII, 2; Hipólito Assunção Antonio MascarenhasI, 2; Carlos RettoriIII

ISeção de Leguminosas, Instituto Agronômico (IAC), Caixa Postal 28, 13001 Campinas, SP
II
Seção de Fitoquímica (IAC)
III
Instituto de Física, UNICAMP, Caixa Postal 109, 13093 Campinas, SP

 

 


RESUMO

Com a finalidade de estudar a possibilidade de realizar seleção recorrente, analisaram-se sementes de soja de plantas macho-estéreis e de sua progênies quanto ao teor de óleo no espectroscópio de ressonância nuclear magnética (NMR), no Instituto de Física da Universidade de Campinas de 1984 a 1988. Tais sementes provinham do composto IAC-1, com macho-esterilidade genética e ampla variabilidade quanto à percentagem de óleo (13,5-22,5%). Determinaram-se os coeficientes de repetibilidade, tomando-se o teor de óleo de cada semente da planta macho-estéril como uma medida fenotípica, obtendo-se o valor médio de r = 0,65. Concluiu-se que com a análise de quatro a cinco sementes, é possível representar a planta-mãe, passo importante para viabilizar a seleção recorrente nessa população, visto o pequeno número de sementes produzidas por planta macho-estéril. Utilizando-se a covariância entre a média das sementes da planta macho-estéril e a média de suas progênies, obteve-se h2 = 0,73. Essa herdabilidade é alta, e como o caráter teor de óleo tem como principal componente de sua variância genética a variância aditiva, pode-se recomendar a seleção fenotípica. Calculou-se também o coeficiente de herdabilidade no nível da média de progênies, obtendo-se valores desde 0,66, para a média de duas plantas por progênie, até 0,85, para a de sete plantas por progênie. Esses resultados demonstram a possibilidade de seleção recorrente no composto IAC-1, tanto no campo, ao nível de planta macho-estéril, como em casa de vegetação, em seleção baseada na média da progênie.

Termos de indexação: melhoramento genético vegetal, soja, óleo vegetal, seleção recorrente, macho-esterilidade, espectroscopia.


SUMMARY

The seed oil content of male sterile plants and their progenies was analised by a NMR spectroscope to study the possibility of recurrent selection to improve the oil content. The seeds derived from a composite named "IAC-1" with genetic male sterility and large variability with respect to oil content. The repeatability coefficient (r) for oil content of each seed of the male sterile plant, was determined as a phenotipic measurement. A value of r = 0.65 was obtained. This value indicates that four or five seeds may represent the parental female plant which is an important step to assure success of recurrent selection in this composite. The value of h2 = 0.73 was obtained for the covariance of average value of male sterile plants and average of progenies. Due to this high value and assuming that the main component of the genetic variability for oil content is of additive nature, this phenotipic selection may be recommended . For the average of progenies the heritability values of 0.66 and 0.85 were obtained for two and seven progenies respectively.These results suggest the possibility of recurrent selection in the composite 'IAC-1' both in the field with male sterile plants or at the greenhouse when selection is based on average values of progenies.

Index terms: plant breeding, soybean, vegetable oil, recurrent selection, genetic male sterility, NMR spectroscopy.


 

 

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Full text available only in PDF format.

 

 

REFERÊNCIAS BIBLIOGRÁFICAS

BERNARD, R.L. & CREMEENS, C.R. Inheritance of the Eldorado malesterile trait. Soybean Genetics Newsletter, Ames, 2:37-39, 1975.        [ Links ]

BRIM, C.A. Quantitative genetics and breeding in soybeans. In: CALDWELL, B.E., ed. Soybeans: improvement, production and uses. Madison, Wisconsin, American Society of Agronomy, 1973. p.155-186.        [ Links ]

__________ & COCKERHAM, C.C. Inheritance of quantitative characters in soybeans. Crop Science, Madison, 1:187-190, 1961.        [ Links ]

BRIM, C.A.; SCHUTZ, W.M. & COLLINS, F.I. Maternal effect on fatty acid composition and oil content of soybeans (Glycine max (L.) Merrill). Crop Science, Madison, 8:517-518, 1968.        [ Links ]

__________; __________ & _________Nuclear magnetic resonance analysis for oil in soybeans (Glycine max (L.) Merrill), with implications in selection. Crop Science, Madison, 7:220-222, 1967.        [ Links ]

__________ & STUBER, C.W. Application of genetic male sterility to recurrent selection schemes in soybeans. Crop Science, Madison, 13:528-530, 1973.        [ Links ]

__________ & YOUNG, M.F. Inheritance of a male-sterile character in soybeans. Crop Science, Madison, 11:564-566, 1971.        [ Links ]

BUSS, G.R. Inheritance of a male-sterile mutant from irradiated Essex soybeans. Soybean Genetics Newsletter, Ames, 10:104-108, 1983.        [ Links ]

BYTH, D.E.; CALDWELL, B.E. & WEBER, C.R. Specific and non-specific index selection in soybean, Glycine max (L.) Merrill. Crop Science, Madison, 9:702-705, 1969.        [ Links ]

COLLINS, F.I.; ALEXANDER, D.E.; RODGERS, R.C. & SIVELA, S.L. Analysis of oil content of soybeans by wide line NMR. Journal of the American Oil Chemists Society, Chicago, 49:708-710, 1967.        [ Links ]

CONWAY, T.F. & EARLE, F.R. Nuclear magnetic resonance for determining oil content of seeds. Journal of the American Oil Chemists Society, Chicago, 40:265-268, 1963.        [ Links ]

DALANNAY, X. & PALMER, R.G. Genetic and cytology of the ms4 male-sterile soybean. Journal of Heredity, Washington, D.C., 73:219-223, 1982.        [ Links ]

DAUGHERTY, D.M.; NEUSTADT, M.H.; GEHRK, C.W.; CAVANAH, L.E.; WILLIAMS, L.F. & GREEN, D.E. An evaluation of damage to soybeans by brown and green stink bugs. Journal of Economic Entomology, Washington, D.C., 57(5):719-722, 1964.        [ Links ]

FALCONER, D.S. Introduction to quantitative genetic. New York, Ronald Press, 1964. 365p.        [ Links ]

FEHR, W.R.; COLLINS, F.I. & WEBER, C.R. Evaluation of methods for protein and oil determination in soybean seed. Crop Science, Madison, 8:47-49, 1968.        [ Links ]

__________& WEBER, C.R. Mass selection by seede size and specific gravity in soybean populations. Crop Science, Madison, 8:551-554, 1968.        [ Links ]

GALILEO, M.H.M. & HEINRICHS, E.A. Avaliação dos danos causados por Piezodorus guildinii (Westwood, 1837) (Hemiptera, Pentatomidae) em diferentes níveis e épocas de infestação, na qualidade de soja (Glycine max (L.) Merrill). Anais da Sociedade de Entomologia do Brasil, Jaboticabal, 7(2):75-88, 1978.        [ Links ]

HANSON, W.D. The breakup of initial linkage bloks under selected mating systems. Genetics, Baltimore, 44:857-868, 1959a.        [ Links ]

__________ Early generation analysis of lengths of heterozygous chromosome segments around a locus held heterozygous with backcrossing or selfing. Genetics, Baltimore, 44:833-837, 1959b.        [ Links ]

__________ Theoretical distribution on the initial linkage blocks lengths intact in the gametes of a population intermated for in generations. Genetics, Baltimore, 44:839-846, 1959c.        [ Links ]

HANSON, W.D. The theoretical distribution on lengths of parental gene blocks in the gametes of an F1 individual. Genetics, Baltimore, 44:197-209, 1959d.        [ Links ]

__________ ; PROBST, A.H. & CALDWELL, B.E. Evaluation of a population of soybean genotypes with implications for improving self-pollinated crops. Crop Sciences, Madison, 7:99-103, 1967.        [ Links ]

__________ & WEBER, C.R. Analysis of genetic variability from generations of plant progeny lines in soybeans. Crop Science, Madison, 1:63-67, 1962.        [ Links ]

__________&__________. Resolution of genetic variability in self-pollinated species with an application to the soybean. Genetics, Baltimore, 46:1425-1434, 1961.        [ Links ]

HARTWIG, E.E.         Varietal development in soybeans. In: CALDWELL, B.E., ed. Soybeans: improvement, production and uses. Madison, American Society of Agronomy, 1973. p.182-210.        [ Links ]

HIROMOTO, D.M. & VELLO, N.A. The genetic base of Brazilian soybean (Glycine max (L.) Merrill) cultivars. Revista Brasileira de Genética, Ribeirão Preto, 9(2):295-306, 1986.        [ Links ]

JOHNSON, H.W. & BERNARD, R.L. Soybean genetic and breeding. In: NORMAN, A.C., ed. The soybean. New York, Academic Press, 1963. p.1-73.        [ Links ]

__________ ; ROBINSON, H.F. & COMSTOCK, R.E. Estimates of genetic and environmental variability in soybeans. Agronomy Journal, Madison, 47:314-318, 1955.        [ Links ]

KWON, S.H. & TORRIE, J.H. Heritability of and interrelationships among traits of two soybean populations. Crop Science, Madison, 4:196-198, 1964.        [ Links ]

LOURENÇÃO, A.L. & MIRANDA, M.A.C. de. Resistência de soja a insetos: VIII. IAC78-2318, linhagem com resistência múltipla. Bragantia, Campinas, 46(1):65-72, 1987.         [ Links ]

__________;__________& NAGAI, V. Resistência de soja a insetos: VII Avaliação de danos de percevejos em cultivares e linhagens. Bragantia, Campinas, 46(1):45-57, 1987.        [ Links ]

MASCARENHAS, H.A.A.; QUAGGIO, J.A.; HIROCE, R.; BRAGA, N.R.; MIRANDA, M.A.C. de & TEIXEIRA, J.P.F. Respostas da soja (Glycine max (L.) Merrill) à aplicação de doses de calcário em solo latossolo roxo distrófico de cerrado: l. Efeito imediato. In: SEMINÁRIO NACIONAL DE PESQUISA DE SOJA, 2., Brasília, 1981. Anais. Londrina, EMBRAPA-CNPSo, 1982. v.2, p.742-751.        [ Links ]

MIRANDA, M.A.C. de; ROSSETTO, C.J.; ROSSETO, D.; BRAGA, N.R.; MASCARENHAS, H.A.A.; TEIXEIRA, J.P.F. & MASSARIOL, A. Resistência de soja a Nezara viridula e Piezodorus guildinii em condições de campo. Bragantia, Campinas, 38:181-188, 1979.        [ Links ]

__________; SUASSUNA FILHO, J.; BULISANI, E.A.; MASCARENHAS, H.A.A.; TISSELLI FILHO, O. & BRAGA, N.R. Efeito maternal e do genótipo sobre o teor de óleo e tamanho de sementes em sementes F1, de soja. In: SEMINÁRIO NACIONAL DE PESQUISA DE SOJA, 3., Campinas, 1984. Anais. Londrina, EMBRAPA-CNPS, 1984. p.308-317.        [ Links ]

PALMER, R.G.; JOHNS, C.W. & MUIR, P.S. Genetic and cytology of the ms3 male-sterile soybean. Journal of Heredity, Washington, D.C., 71:343-348, 1980.        [ Links ]

SHORTER, R.; BYTH, D.E. & MUNGOMERY, V.E. Estimates of selection parameters associated with protein and soil content of soybean, seeds Glycine max (L.) Merrill. Australian Journal of Agricultural Research, Melbourne, 28:211-222, 1976.        [ Links ]

SINGH, D.B. & HADLEY, H.H. Maternal control of oil synthesis in soybeans, Glycine max (L.) Merrill. Crop Science, Madison, 8:622-624, 1968.        [ Links ]

SMITH, R.R. & WEBER, C.R. Mass selection by specific gravity for protein and oil in soybean populations. Crop Science, Madison, 8:373-377, 1968.        [ Links ]

TEIXEIRA, J.P.F.; RAMOS, M.T.B.; MIRANDA, M.A.C. de & MASCARENHAS. H.A.A. Relação entre os principais constituintes químicos de grãos de soja. In: SEMINÁRIO NACIONAL DE PESQUISA DE SOJA, 3., Campinas, 1984. Anais. Londrina, EMBRAPA-CNPS, 1984.        [ Links ]

THOMAS, G.D.; IGNOFFO, C.M.; MORGAN, CE. & DICKERSON, W.A. Southern green stink bug: influence on yield and quality of soybeans. Journal of Economic Entomology, Washington, D.C., 67(4):501-503, 1974.        [ Links ]

TODD, J.W. & TURNIPSEED, S.G. Effects of sourthern green stink bug damage on yield and quality of soybeans. Journal of Economic Entomology, Washington, D.C, 67(3):421-426, 1974.        [ Links ]

UNITED STATES. Department of Agriculture. Agricultural statistics. Washington, D.C, U.S. Govet Printing Office, 1982.        [ Links ]

VENCOVSKY, R. Herança quantitativa. In: PATERNIANI, E. & VIÉGAS, G.P., eds. Melhoramento e produção do milho. Campinas, Fundação Cargill, 1987. p.137-212.        [ Links ]

__________ Repetibilidade. In: PRINCÍPIOS DE GENÉTICA QUANTITATIVA. Piracicaba, ESALQ, Departamento de Genética, 1977. p.47-52. (Publicação didática)        [ Links ]

WEBER, C.R. & MOORTHY, B.R. Heritable and nonheritable relationships and variability of oil content and agronomic characteristics in F2 generation of soybean crosses. Agronomy Journal, Madison, 44:202-209, 1952.        [ Links ]

 

 

Recebido para publicação em 5 de janeiro e aceito em 19 de junho de 1989

 

 

1 Trabalho apresentado na "World Soybean Research Conference IV", realizada em Buenos Aires, Argentina, em 5-9 de março de 1989. Parcialmente financiado pelo projeto IAC/FUNDEPAG/FINEP. Melhoramento Genético da Soja para Aumento do Teor de óleo.
2 Com bolsa de pesquisa do CNPq.

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