Efeito de diferentes concentrações do meio MS, nitrogênio e sacarose na micropropagação de crisântemo 'orange reagen'

Different concentrations of the ms medium, nitrogen and sucrose on micropropagation of chrysanthemum

I. BIOTECNOLOGIA, BOTÂNICA E FITOQUÍMICA

Efeito de diferentes concentrações do meio MS, nitrogênio e sacarose na micropropagação de crisântemo 'orange reagen'¹

Different concentrations of the ms medium, nitrogen and sucrose on micropropagation of chrysanthemum

Patrícia Duarte de Oliveira^I; Moacir Pasqual^I; Renato Paiva^II

^IDepartamento de Agricultura, UFLA, Caixa Postal 37, 37200-000 Lavras (MG)

^IIProfessor Adjunto, Departamento de Biologia, UFLA

RESUMO

Realizaram-se experimentos objetivando estabelecer concentrações satisfatórias do meio básico a ser utilizado, Murashige e Skoog (MS), de nitrogênio e de sacarose para a propagação "in vitro" de crisântemo (Dendranthema grandiflora Tzvelev.) cv. Orange Reagen. Testaram-se as concentrações 0, 25, 50, 75, 100, 125 e 150% do meio MS combinadas com 0; 0,75; 1,5; 3,0; 6,0 e 12,0% de sacarose. Num segundo experimento, as concentrações 0, 25, 50, 75, 100, 125 e 150% de nitrogênio (em relação ao MS) foram testadas em combinação com as mesmas concentrações de sacarose. Observou-se melhor desenvolvimento de brotos em meios com concentrações entre 50 e 150%, em relação ao MS, combinados com 6% de sacarose. Na concentração de 50% do MS, o tamanho dos brotos obtidos não foi satisfatório. Concentrações elevadas de meio de cultura inibiram a formação de raízes. Verificaram-se resultados semelhantes para as concentrações testadas de nitrogênio. Pode-se então recomendar a utilização de 75% do meio MS com 75% de nitrogênio, acrescido de 6% de sacarose.

Termos de indexação: cultura de tecidos, crisântemo, Dendranthema grandiflora Tzvelev., meio MS, nitrogênio, sacarose.

ABSTRACT

The effect of MS medium, nitrogen and sucrose war investigated on "in vitro" propagation of shoot nodal explants of chrysanthemum (Dendranthema grandiflora Tzvelev.) cultivars Orange Reagen. MS medium was tested using the following concentrations: 0, 25, 50, 75, 100, 125 and 150% in combination with sucrose doses of: 0, 0.75, 1.5, 3.0, 6.0 and 12.0%. Nitrogen was tested using the concentrations of 0, 25, 50, 75, 100, 125 and 150% in combination with the sucrose doses used as above. Best shoot development was observed when nodal segments were cultivated on medium containing concentrations between 50 and 150% of MS in combination with 6% sucrose. No satisfactory shoot length was observed when 50% MS was used. Roots were not observed at concentrations above 100% of MS and nitrogen. In order to obtain successful "in vitro" propagation of chrysanthemum, our results suggest the use of 75% MS, with 75% of nitrogen and 6% of sucrose.

Index terms: tissue culture, chrysanthemum, Dendranthema grandiflora Tzvelev., MS medium, nitrogen, sucrose.

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Recebido em 15 de fevereiro e aceito para publicação em 30 de outubro de 1995.

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Parte da dissertação de mestrado do primeiro autor, apresentada na Universidade Federal de Lavras (UFLA), em 30 de setembro de 1994.

Datas de Publicação

• Publicação nesta coleção
  17 Out 2007
• Data do Fascículo
  1996

Histórico

• Recebido
  15 Fev 1995
• Aceito
  30 Out 1995