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Relative competitiveness of soybean cultivars with barnyardgrass

ABSTRACT

The aim of this work was to evaluate the competitiveness between soybean cultivars and barnyardgrass, based on morphological and physiological characteristics of species. The experiments were conducted in completely randomized experimental design, with 4 replications. In the first study, for both soybean and barnyardgrass, it was determined the population of plants in which shoot dry matter became constant and independent of the population (16 plants∙pot−1 or 400 plants∙m−2). In the second study, 2 experiments were conducted to evaluate the competitiveness of BMX Apolo RR and BMX Potência RR soybean cultivars with barnyardgrass plants, both carried out in replacement series under different proportions of plants∙pot−1 (100:0; 75:25; 50:50; 25:75 and 0:100) between the crop and the weed. The analysis of the species competitiveness was determined through diagrams applied to replacement series experiments and use of relative competitiveness indexes. At 44 days after the emergence of species, the physiological and morphological parameters of the crop and the weed were evaluated. The BMX Apolo RR and BMX Potência RR soybean cultivars show similar competitiveness when competing with the barnyardgrass; therefore, the ability of one species to interfere on another is equivalent. For plant height, barnyardgrass displays higher competitiveness compared to BMX Apolo RR, with early cycle and short height. The intraspecific competition is more important to barnyardgrass than interspecific competition with soybean cultivars, resulting in negative effects on the morphological and physiological characteristics of species.

Keys words
Echinochloa crus-galli; lowlands; replacement series; competition

RESUMO

O objetivo do trabalho foi avaliar a competitividade relativa de cultivares de soja com o capim-arroz, com base em caraterísticas morfofisiológicas das espécies. Os experimentos foram conduzidos em delineamento experimental inteiramente casualizado, com 4 repetições. No primeiro estudo, tanto para soja quanto para capim-arroz, determinou-se a população de plantas em que a matéria seca da parte aérea tornou-se constante e independente da população (16 plantas∙vaso−1 ou 400 plantas∙m−2). No segundo estudo, foram conduzidos 2 experimentos para avaliar a competitividade dos cultivares de soja BMX Apolo RR e BMX Potência RR com plantas de capim-arroz, ambos conduzidos em série de substituição, sob diferentes proporções de plantas∙vaso−1 (100:0; 75:25; 50:50; 25:75 e 0:100) entre a cultura e a planta daninha. A análise da competitividade foi efetuada por meio de diagramas aplicados a experimentos substitutivos e uso de índices de competitividade relativa. Aos 44 dias após a emergência das espécies, efetuou-se a aferição dos parâmetros fisiológicos e morfológicos da cultura e planta daninha. Os cultivares de soja BMX Apolo RR e BMX Potência RR apresentam similaridade quanto à competição com o capim-arroz, de modo que a habilidade de uma espécie em interferir sobre a outra é equivalente. Para a estatura de plantas, o capim-arroz apresenta superioridade competitiva em relação ao cultivar de soja BMX Apolo RR, de ciclo superprecoce e porte baixo. A competição intraespecífica é mais importante para o capim-arroz do que a competição interespecífica com os cultivares de soja, resultando em efeitos negativos sobre características morfofisiológicas da espécie.

Palavras-chave
Echinochloa crus-galli; terras baixas; série de substituição; competição

INTRODUCTION

Barnyardgrass (Echinochloa crus-galli L.) is a highly-competitive weed, especially due to C4 photosynthetic cycle, rapid initial growth combined with high demand for nitrogen, and the fact that it is present in high infestation levels in lowland areas (Kissmann 2007Kissmann, K. G. (2007). Plantas infestantes e nocivas. Tomo I. 3. ed. São Paulo: Basf Brasileira S. A. CD-ROM.). Soybean crops have expanded in these areas, integrating the crop rotation systems, especially with rice; however, barnyardgrass infestations have become important in soybean crops affecting grain yield when not properly managed.

Competition between plants arises when one (or more) of the resources essential to growth/development is present in limited quantity to meet the requirements of individuals. However, this competition can occur between individuals of the same species (intraspecific) or between individuals of different species (interspecific) (Rigoli et al. 2008Rigoli, R. P., Agostinetto, D., Schaedler, C. E., Dal Magro, T. and Tironi, S. (2008). Habilidade competitiva relativa do trigo (Triticum aestivum) em convivência com azevém (Lolium multiflorum) ou nabo (Raphanus raphanistrum). Planta Daninha, 26, 93-100. http://dx.doi.org/10.1590/S0100-83582008000100010.
http://dx.doi.org/10.1590/S0100-83582008...
). In the environment, plants compete basically for water, light, nutrients, O2 and CO2 to a lesser extent (Radosevich et al. 2007Radosevich, S. R., Holt, J. and Ghersa, C. (2007). Ecology of weeds and invasive plants: relationship to agriculture and natural resource management. 3. ed. New Jersey: Wiley-Interscience.).

There are several methods to investigate the competitive relationships between plants; among them, the experiments in replacement series stand out, which have been widely used for studies of different crop/weeds (Aminpanah and Javadi 2011Aminpanah, H. and Javadi, M. (2011). Competitive ability of two rice cultivars (Oryza sativa L.) with barnyardgrass (Echinochloa crus-galli (L.) p. beauv.) in a replacement series study. Advances in Environmental Biology, 5, 2669-2675.; Bianchi et al. 2006Bianchi, M. A., Fleck, N. G. and Lamego, F. P. (2006). Proporção entre plantas de soja e plantas competidoras e as relações de interferência mútua. Ciência Rural, 36, 1380-1387. http://dx.doi.org/10.1590/S0103-84782006000500006.
http://dx.doi.org/10.1590/S0103-84782006...
; Galon et al. 2014Galon, L., Guimarães, S., Lima, A. M., Radunz, A. L., Beutler, A. N., Burg, G. M., Zandoná, R. R., Perin, G. F., Bastiani, M. O., Belarmino, J. G. and Radunz, L. L. (2014). Interação competitiva de genótipos de arroz e papuã. Planta Daninha, 32, 533-542. http://dx.doi.org/10.1590/S0100-83582014000300009.
http://dx.doi.org/10.1590/S0100-83582014...
; Rigoli et al. 2008Rigoli, R. P., Agostinetto, D., Schaedler, C. E., Dal Magro, T. and Tironi, S. (2008). Habilidade competitiva relativa do trigo (Triticum aestivum) em convivência com azevém (Lolium multiflorum) ou nabo (Raphanus raphanistrum). Planta Daninha, 26, 93-100. http://dx.doi.org/10.1590/S0100-83582008000100010.
http://dx.doi.org/10.1590/S0100-83582008...
; Wandscheer and Rizzardi 2013Wandscheer, A. C. D. and Rizzardi, M. A. (2013). Interference of soybean and corn with Chloris distichophylla. Ciência e Agrotecnologia, 37, 306-312. http://dx.doi.org/10.1590/S1413-70542013000400003.
http://dx.doi.org/10.1590/S1413-70542013...
). This method allows the understanding of the competitive process between plants, especially when related to the effect of the population as well as of the crop and weeds proportion (Aminpanah and Javadi 2011Aminpanah, H. and Javadi, M. (2011). Competitive ability of two rice cultivars (Oryza sativa L.) with barnyardgrass (Echinochloa crus-galli (L.) p. beauv.) in a replacement series study. Advances in Environmental Biology, 5, 2669-2675.).

The replacement or substitutive series experimental model was first proposed by Radosevich (1987)Radosevich, S. R. (1987). Methods to study interactions among crops and weeds. Weed Technology, 1, 190-198., where the plant total density is kept constant, while varying the mixing proportions of the species. This situation is different from that found in field conditions where crop density is constant while weed density is variable. However, the experimental model is important to assess the effects of competition of the 2 species for a total single density and to determine the relative effects of interference within and between species, indicating which one is more competitive.

Studies in substitutive series have found that usually crops have higher competitive ability than weeds (Agostinetto et al. 2013Agostinetto, D., Fontana, L. C., Vargas, L., Markus, C. and Oliveira, E. (2013). Habilidade competitiva relativa de milhã em convivência com arroz irrigado e soja. Pesquisa Agropecuária Brasileira, 48, 1315-1322. http://dx.doi.org/10.1590/S0100-204X2013001000002.
http://dx.doi.org/10.1590/S0100-204X2013...
; Galon et al. 2014Galon, L., Guimarães, S., Lima, A. M., Radunz, A. L., Beutler, A. N., Burg, G. M., Zandoná, R. R., Perin, G. F., Bastiani, M. O., Belarmino, J. G. and Radunz, L. L. (2014). Interação competitiva de genótipos de arroz e papuã. Planta Daninha, 32, 533-542. http://dx.doi.org/10.1590/S0100-83582014000300009.
http://dx.doi.org/10.1590/S0100-83582014...
; Moraes et al. 2009Moraes, P. V. D., Agostinetto, D., Galon, L. and Rigoli, R. P. (2009). Competitividade relativa de soja com arroz-vermelho. Planta Daninha, 27, 35-40. http://dx.doi.org/10.1590/S0100-83582009000100006.
http://dx.doi.org/10.1590/S0100-83582009...
). This behavior is explained by the fact that the negative effect of weeds is not restricted by the largest individual competitive ability, but mainly due to the level of infestation in the area (Vilà et al. 2004Vilà, M., Williamson, M. and Lonsdale, M. (2004). Competition experiments on alien weeds with crops: lessons for measuring plant invasion impact? Biological Invasions, 6, 59-69. http://dx.doi.org/10.1023/B:BINV.0000010122.77024.8a.
http://dx.doi.org/10.1023/B:BINV.0000010...
).

Research using replacement series studies has shown that soybean crop has competitive advantage in relation to weeds, grasses and broadleaves (Bianchi et al. 2006Bianchi, M. A., Fleck, N. G. and Lamego, F. P. (2006). Proporção entre plantas de soja e plantas competidoras e as relações de interferência mútua. Ciência Rural, 36, 1380-1387. http://dx.doi.org/10.1590/S0103-84782006000500006.
http://dx.doi.org/10.1590/S0103-84782006...
; Dias et al. 2010Dias, A. C. R., Carvalho, S. J. P., Marcolini, L. W., Melo, M. S. C. and Christoffoleti, P. J. (2010). Competitiveness of alexandergrass or Bengal dayflower with soybean. Planta Daninha, 28, 515-522. http://dx.doi.org/10.1590/S0100-83582010000300008.
http://dx.doi.org/10.1590/S0100-83582010...
; Moraes et al. 2009Moraes, P. V. D., Agostinetto, D., Galon, L. and Rigoli, R. P. (2009). Competitividade relativa de soja com arroz-vermelho. Planta Daninha, 27, 35-40. http://dx.doi.org/10.1590/S0100-83582009000100006.
http://dx.doi.org/10.1590/S0100-83582009...
; Wandscheer and Rizzardi 2013Wandscheer, A. C. D. and Rizzardi, M. A. (2013). Interference of soybean and corn with Chloris distichophylla. Ciência e Agrotecnologia, 37, 306-312. http://dx.doi.org/10.1590/S1413-70542013000400003.
http://dx.doi.org/10.1590/S1413-70542013...
). However, the results for barnyardgrass have shown that this weed usually has competitive advantage in relation to irrigated rice cultivars (Agostinetto et al. 2008Agostinetto, D., Galon, L., Moraes, P. V. D., Rigoli, R. P., Tironi, S. P. and Panozzo, L. E (2008). Competitividade relativa entre cultivares de arroz irrigado e biótipo de capim-arroz (Echinochloa spp.). Planta Daninha, 26, 757-766. http://dx.doi.org/10.1590/S0100-83582008000400007.
http://dx.doi.org/10.1590/S0100-83582008...
; Aminpanah et al. 2012Aminpanah, H., Sharifi, P. and Firouzi, S. (2012). Interference interactions of two species of Echinochloa genus with rice. Chilean journal of agricultural research, 72, 364-370. http://dx.doi.org/10.4067/S0718-58392012000300010.
http://dx.doi.org/10.4067/S0718-58392012...
; Gealy et al. 2005Gealy, D. R., Estorninos JR, L. E., Gbur, E. E. and Chavez, R. S. C. (2005). Interference interactions of two rice cultivars and their F3 cross with barnyardgrass (Echinochloa crus-galli) in a replacement series study. Weed Science, 53, 323-330. http://dx.doi.org/10.1614/WS-04-036R.
http://dx.doi.org/10.1614/WS-04-036R...
), and this response is attributed to the high morphophysiological similarity between the species. Thus, both of them end up exploiting basically the same ecological niche, competing for the same resources in time and/or space (Agostinetto et al. 2008Agostinetto, D., Galon, L., Moraes, P. V. D., Rigoli, R. P., Tironi, S. P. and Panozzo, L. E (2008). Competitividade relativa entre cultivares de arroz irrigado e biótipo de capim-arroz (Echinochloa spp.). Planta Daninha, 26, 757-766. http://dx.doi.org/10.1590/S0100-83582008000400007.
http://dx.doi.org/10.1590/S0100-83582008...
).

This study hypothesizes that barnyardgrass is potentially more competitive than soybean when occurring in similar proportions and with appropriate resource levels, especially due to more efficient photosynthetic cycle and rapid initial growth. Thus, the objective of this study was to evaluate the competitiveness between soybean cultivars and barnyardgrass, based on morphological and physiological characteristics of these species.

MATERIAL AND METHODS

The research was divided into 2 studies (Study 1 and 2). The Study 2 was divided into 2 separate experiments. Both were carried out in greenhouses. Study 1 was conducted from October to December 2013 while Study 2, from December 2013 to February 2014. The experimental units were 8-L plastic pots with 23 cm of nominal diameter, filled with soil, classified as Albaqualf, with sandy loam texture, from mapping unit of Pelotas.

Study 1 consisted of soybean monoculture and barnyardgrass, aiming to determine the population of plants per m2 in which the shoot dry matter (SDM) per unit area (g∙m−2) became constant and independent of the population based on the “law of constant final production” (Radosevich et al. 2007Radosevich, S. R., Holt, J. and Ghersa, C. (2007). Ecology of weeds and invasive plants: relationship to agriculture and natural resource management. 3. ed. New Jersey: Wiley-Interscience.). The hypothesis proposed by these authors is that the population per pot when the dry matter weight becomes constant is sufficient to capture all the necessary resources for plant growth.

The experimental design was completely randomized, with 4 replications. In this previous experiment, soybean (cv. BMX Potência RR) and barnyardgrass were sown as monocultures to establish the following plant populations: 1; 2; 4; 8; 16; 32; 64 and 128 plants∙pot−1 (equivalent to 25; 50; 100; 200; 400; 800; 1,600 and 3,200 plants∙m−2) for both species. The seeds of the barnyardgrass biotype (Echinochloa crusgalli (L.) Beauv. var. crusgalli) were previously collected in the field (lat 31°48′25.4″ and long 52°28′53.4″), shortly after the beginning of the natural dispersion in May 2013. Then, they were manually cleaned and stored in paper bags at room temperature until the beginning of the experiments.

Forty-four days after emergence (DAE), the shoots were harvested, packaged in paper bags and dried in a forced air circulation oven, at 65 °C average, until constant weight of SDM.

For data analysis, reciprocal production was used to determine the plant population in which SDM became constant (Radosevich et al. 2007Radosevich, S. R., Holt, J. and Ghersa, C. (2007). Ecology of weeds and invasive plants: relationship to agriculture and natural resource management. 3. ed. New Jersey: Wiley-Interscience.). The SDM became constant from the population of 16 plants∙pot−1 onward (data not shown), equivalent to 400 plants∙m−2.

Study 2 was conducted based on the replacement series model described by Radosevich (1987)Radosevich, S. R. (1987). Methods to study interactions among crops and weeds. Weed Technology, 1, 190-198., with the number of plants per pot (16 plants∙pot−1) established in Study 1. The experimental design was completely randomized, with 4 repetitions. The proportions between BMX Potência RR soybean cultivar and barnyardgrass (Experiment 1) and between BMX Apolo RR soybean cultivar and barnyardgrass (Experiment 2), in each series, were: 100:0 (soybean monoculture), 75:25; 50:50; 25:75 and 0:100 (barnyardgrass monoculture).

The variables evaluated at 44 DAE were: plant height (PH), leaf area (LA), SDM and photosynthetic parameters. The plant height was determined using a millimeter ruler to measure the height from the ground to the apex of the last expanded trifoliate for soybeans and the height from the ground to the end of the last leaf to barnyardgrass. Leaf area (cm2∙plant−1) was determined using a leaf area meter LI 3100C (LI-COR, Lincoln, NE, USA), and SDM (g∙plant−1) was quantified as described above.

The photosynthetic parameters were evaluated in the last expanded trefoil of soybean plants and in the middle third of the first fully expanded leaf of the barnyardgrass, using an infrared gas analyzer (IRGA), model LI-6400 (LI-COR, Lincoln, NE, USA). The greenhouse was kept open to allow free air circulation during the measurements. This equipment was also used to determine stomatal conductance (Gs: mol∙m−1∙s−1), transpiration rate (E: mol H2O∙m−2∙s−1), intercellular CO2 concentration (Ci: µmol∙mol−1) and photosynthetic rate (A: µmol∙m−2∙s−1). The efficiency of carboxylation (EC: mol CO2∙m−2∙s−1) and water use efficiency (WUE: mol CO2∙mol H2O−1) were calculated from the ratio of the variables A/Ci and A/E, respectively.

For the variables plant height, leaf area and SDM of soybean cultivars and weed, it was used the method of graphical analysis of the relative yield (Radosevich 1987Radosevich, S. R. (1987). Methods to study interactions among crops and weeds. Weed Technology, 1, 190-198.). This model involves the construction of a diagram based on the relative yield (RY) and relative total yield (RTY) for the studied plant proportions (0; 25; 50; 75 and 100%).

The RY values were obtained according to the following equation: RY = mixing average/monoculture average, where “average” represents the average per plant of each species in each experimental unit. The RTY data were represented by the sum of the relative yield of culture and the competitors in the respective plant proportions. When RY is a straight line, there is no effect of one species over the other, or the ability of the species to interfere with one another is equivalent. When RY is a concave curve, the growth of one or both species was negatively affected; however, a convex curve indicates that the growth of one or both species was positively affected. On the other hand, RTY equal to 1 (straight line) means that there was competition for the same resources. However, a value greater than 1 (convex line) means that there was no competition, either because the supply of resources exceed the demand or because the demands for the resources are different for each species. Finally, a value less than 1 (concave) indicates the occurrence of antagonism that harmed the growth of both species (Radosevich 1987Radosevich, S. R. (1987). Methods to study interactions among crops and weeds. Weed Technology, 1, 190-198.).

Relative competitiveness (RC) indices as well as coefficients of relative crowding (K) and of competitiveness (C) were calculated for the 50% proportion (crop and competitor). The RC is the comparative growth of species X in relation to Y; K indicates the relative dominance of one species over another; and C shows which species is more aggressive (Cousens 1991Cousens, R. (1991). Aspects of the design and interpretation of competition (interference) experiments. Weed Technology, 5, 664-673.). The joint interpretation of these values (CR, K and C) indicates, with higher certainty, the competitiveness of the species involved. Accordingly, the species X is more competitive than Y when RC > 1; Kx > Ky and C > 0; otherwise, Y will be more competitive than X when RC < 1; Kx < Ky and C < 0 (Hoffman and Buhler 2002Hoffman, M. L. and Buhler, D. D. (2002). Utilizing Sorghum as functional model of crop-weed competition. I. Establishing a competitive hierarchy. Weed Science, 50, 466-472. http://dx.doi.org/10.1614/0043-1745(2002)050[0466:USAAFM]2.0.CO;2.
http://dx.doi.org/10.1614/0043-1745(2002...
). These indices were calculated using the following equations: RC = RYx/RYy; Kx = RYx/(1 − RYx); Ky = RYy/(1 − RYy); C = RYx − RYy, according to Cousens and O’Neill (1993)Cousens, R. and O’Neill, M. (1993). Density dependence of replacement series experiments. Oikos, 66, 347-352. http://dx.doi.org/10.2307/3544824.
http://dx.doi.org/10.2307/3544824...
.

The differences of RY values for the 25, 50 and 75% proportions were first calculated in relation to the values of the hypothetical straight lines obtained in the respective proportions to analyze the relative yield statistically (Bianchi et al. 2006Bianchi, M. A., Fleck, N. G. and Lamego, F. P. (2006). Proporção entre plantas de soja e plantas competidoras e as relações de interferência mútua. Ciência Rural, 36, 1380-1387. http://dx.doi.org/10.1590/S0103-84782006000500006.
http://dx.doi.org/10.1590/S0103-84782006...
). The t-test at 5% probability was used to test differences in the studied indices in relation to the hypothetical straight line (Hoffman and Buhler 2002Hoffman, M. L. and Buhler, D. D. (2002). Utilizing Sorghum as functional model of crop-weed competition. I. Establishing a competitive hierarchy. Weed Science, 50, 466-472. http://dx.doi.org/10.1614/0043-1745(2002)050[0466:USAAFM]2.0.CO;2.
http://dx.doi.org/10.1614/0043-1745(2002...
; Roush et al. 1989Roush, M. L., Radosevich, S. R., Wagner, R. G., Maxwell, B. D. and Petersen, T. D. (1989). A comparison of methods for measuring effects of density and proportion in plant competition experiments. Weed Science, 37, 268-275.). The null hypotheses used to test differences of RY and C were made on the assumption that, if they were equal to zero (H0 = 0), for RTY and RC, the averages would be equal to 1 (H0 = 1); for the K index, the mean differences between Kx and Ky were equal to zero: H0 = (Kx − Ky) = 0.

The results obtained for the morphological (PH, LA, SDM) and photosynthetic (A, Gs, Ci, E, EC and WUE) characteristics of soybean and barnyardgrass were expressed as average values per plant. Firstly, the data were tested for normality by the Shapiro-Wilk’s test (p ≥ 0.05), without the need for data transformation. Later, analysis of variance was performed by the F-test (p ≤ 0.05) and, in the case of significant difference between the treatments, the means were compared by the Dunnett’s test (p ≤ 0.05), considering monocultures as untreated check in such comparisons. For photosynthetic parameters, the Duncan’s test (p ≤ 0.05) was also applied to analyze the differences between the species proportions.

RESULTS AND DISCUSSION

The graphical analysis of the proportions of soybean (BMX Apolo RR and BMX Potência RR) and the competitor, barnyardgrass, showed that, in general, the expected RY values were close to those observed, revealing that the 2 cultivars responded similarly to the weed competition (Figure 1; Table 1). For the difference to be significant, at least 2 plant proportions should differ significantly (Bianchi et al. 2006Bianchi, M. A., Fleck, N. G. and Lamego, F. P. (2006). Proporção entre plantas de soja e plantas competidoras e as relações de interferência mútua. Ciência Rural, 36, 1380-1387. http://dx.doi.org/10.1590/S0103-84782006000500006.
http://dx.doi.org/10.1590/S0103-84782006...
). Thus, based on the RY, only the height of BMX Apolo RR soybean cultivar was different for at least 2 plant proportions (75:25 and 50:50), represented by a concave curve (Figure 1a; Table 1). In this case, it was demonstrated that barnyardgrass used the available resources more efficiently than the soybean, without changing the RY, while, for the BMX Apolo RR, RY decreased as crop population increased.

Figure 1
Relative production diagram for plant height, leaf area and shoot dry matter of BMX Apolo RR (a; b; c) and BMX Potência RR (d; e; f) soybean cultivars and barnyardgrass (Echinochloa crus -galli) as a function of the proportion between the 2 species.
Table 1
Relative yield differences and relative total yield for the variables plant height, leaf area and shoot dry matter of soybean cultivars (BMX Apolo RR or BMX Potência RR) and barnyardgrass (Echinochloa crus-galli) at 44 days after emergence.

RTY and RY behaved similarly since the expected straight lines had values very close to the unit (1), with no statistical difference (p ≤ 0.05). The exception was the plant height of BMX Potência RR, which, in the 50:50 and 25:75 proportions, had RTY equivalent to less than 1, represented by a concave curve (Figure 1d; Table 1). For this, according to Harper (1977)Harper, J. L. (1977). The population biology of plants. London: Academic Press., there was antagonism between species since RTY decreased due to the lower contribution of both species, i.e. the 2 species competed for the same resources.

The analysis of the RY and RTY figures shows that the observed and expected lines are very close to the unit (1), with no differences in most cases (Figure 1; Table 1). Generally, it is possible to infer that the effect of one species over another in the competition for environmental resources was low, and probably the ability of one species to interfere with the other was equivalent (Radosevich 1987Radosevich, S. R. (1987). Methods to study interactions among crops and weeds. Weed Technology, 1, 190-198.).

Similar results were reported for the competition between corn and Chloris distichophylla, where the observed and hypothetical values did not differ. The authors inferred that, even with weed interference, corn maintained yield equivalent to monoculture condition (Wandscheer and Rizzardi 2013Wandscheer, A. C. D. and Rizzardi, M. A. (2013). Interference of soybean and corn with Chloris distichophylla. Ciência e Agrotecnologia, 37, 306-312. http://dx.doi.org/10.1590/S1413-70542013000400003.
http://dx.doi.org/10.1590/S1413-70542013...
). Likewise, it was observed that the competitive ability of Commelina benghalensis and soybeans were equivalent, with no effect on overall yield of either species (Dias et al. 2010Dias, A. C. R., Carvalho, S. J. P., Marcolini, L. W., Melo, M. S. C. and Christoffoleti, P. J. (2010). Competitiveness of alexandergrass or Bengal dayflower with soybean. Planta Daninha, 28, 515-522. http://dx.doi.org/10.1590/S0100-83582010000300008.
http://dx.doi.org/10.1590/S0100-83582010...
).

It is noteworthy that, for all variables tested in both cultivars, for the proportion of 75:25 (soybean/competitor-weed), the RY soybean values were always below the hypothetical straight line, while RY weed values were, in general, higher than expected (Figure 1). On the other hand, these values were not always significantly different (p ≤ 0.05) (Table 1). However, this trend is a result of increased intraspecific competition for both soybean and barnyardgrass. Soybean has lower relative yield at higher proportions, but it is not affected by the increase of competitor population, while the competitor yield is higher than expected at lower proportions.

The relative growth of soybean cultivars and barnyardgrass analyzed by the morphological variables (PH, LA and SDM) showed that both cultivars did not differ (p ≤ 0.05) from the untreated check (monoculture – 100:0) for all studied proportions (Table 2).

Table 2
Soybean (BMX Apolo RR and BMX Potência RR cultivars) responses to the interference of barnyardgrass (Echinochloa crus-galli) for the variables plant height, leaf area and shoot dry matter, in experiments conducted in substitutive series at 44 days after emergence.

The lowest proportion of barnyardgrass, combined with BMX Apolo RR cultivar, had the highest SDM. On the other hand, when competing with BMX Potência RR in the same proportion, the competitor presented increased values for PH, LA and SDM (Table 2). This behavior is again the result of increased intraspecific competition, especially for barnyardgrass. Thus, several replacement series studies have reported that, in some cases, the intraspecific competition seems to be more important than the interspecific one, because plants of the same species basically exploit the same ecological niche, competing for the same resources in time and/or space (Dias et al. 2010Dias, A. C. R., Carvalho, S. J. P., Marcolini, L. W., Melo, M. S. C. and Christoffoleti, P. J. (2010). Competitiveness of alexandergrass or Bengal dayflower with soybean. Planta Daninha, 28, 515-522. http://dx.doi.org/10.1590/S0100-83582010000300008.
http://dx.doi.org/10.1590/S0100-83582010...
; Moraes et al. 2009Moraes, P. V. D., Agostinetto, D., Galon, L. and Rigoli, R. P. (2009). Competitividade relativa de soja com arroz-vermelho. Planta Daninha, 27, 35-40. http://dx.doi.org/10.1590/S0100-83582009000100006.
http://dx.doi.org/10.1590/S0100-83582009...
; Rigoli et al. 2008Rigoli, R. P., Agostinetto, D., Schaedler, C. E., Dal Magro, T. and Tironi, S. (2008). Habilidade competitiva relativa do trigo (Triticum aestivum) em convivência com azevém (Lolium multiflorum) ou nabo (Raphanus raphanistrum). Planta Daninha, 26, 93-100. http://dx.doi.org/10.1590/S0100-83582008000100010.
http://dx.doi.org/10.1590/S0100-83582008...
).

The analysis of the physiological parameters shows that the higher relative growth of the weed at the lowest proportion (25%), previously presented by morphological variables (Figure 1; Table 1,2), is due, in part, to the increased photosynthetic activity and related variables (Table 3). In this sense, there was a higher photosynthetic rate (A) of barnyardgrass when soybean proportion was higher, regardless of the cultivar tested (Table 3). For the same proportion, the effects were more pronounced when the competitor was competing with BMX Potência RR cultivar. In addition to increased photosynthetic rate, the values for stomatal conductance (Gs), transpiration rate (E) and efficiency of carboxylation (EC) also increased. At this proportion (75:25), the plants differed from the untreated check, and the values were also higher compared to the other proportions (50:50 and 25:75). On the other hand, both soybean cultivars were not different regarding the physiological variables in relation to the competitor presence (Table 3).

Table 3
Soybean (BMX Apolo RR and BMX Potência RR cultivars) responses to the interference of barnyardgrass (Echinochloa crus-galli) for the variables photosynthetic rate, stomatal conductance of water vapor, internal CO2 concentration, transpiration rate, efficiency of carboxylation and water use efficiency of the plants in experiments conducted in substitutive series at 44 days after emergence.

The results indicate a connection between morphological and physiological variables that allows us to better understand the behavior of the studied species (Table 2,3). Thus, in proportion with less intraspecific competition (75:25) by the barnyardgrass, further opening of stomatal pores enabled the plant to incorporate more atmospheric CO2 into the cells and, consequently, to lose more water to atmosphere resulting in increasing transpiration rate (E). Probably, these CO2 molecules incorporated into the interior of mesophyll cells were not stored for a long time, because the internal CO2 concentration (Ci) did not change regardless of the plant’s proportions. Thus, it is proposed that CO2 was quickly carboxylated and transformed into glucose, confirmed here by the increase in photosynthetic activity (A) and efficiency of carboxylation (EC).

The increase in transpiration rate by barnyardgrass was not enough to result in lower water use efficiency. The greatest water loss may result from the need of the plant to incorporate CO2 by photosynthetic activity and carboxylation efficiency (Concenço et al. 2009Concenço, G., Sant’anna, S. J., Schwanke, A. M. L., Galon, L., Ferreira, E. A., Aspiazú, I., Silva, A. F. and Ferreira, F. A. (2009). Uso da água por plantas híbridas ou convencionais de arroz Irrigado. Planta Daninha, 27, 447-453. http://dx.doi.org/10.1590/S0100-83582009000300004.
http://dx.doi.org/10.1590/S0100-83582009...
). These authors reported similar results, where rice plants under lower intensity competition, in addition to greater dry matter accumulation, also displayed higher values of stomatal conductance and transpiration rate. However, the water use efficiency remained unchanged for different competition intensities.

In general, it was observed that, when competing with BMX Apolo RR, barnyardgrass had higher relative growth only for plant height, as shown by RC index (Table 4). However, the dominant competitor was observed for both soybean cultivars, as indicated by the index K. For plant height, barnyardgrass was more competitive than BMX Apolo RR cultivar as shown by the A index (Table 4). This is based on the assumption that species Y is more competitive than X when RC < 1; Kx < Ky and C < 0 (Hoffman and Buhler 2002Hoffman, M. L. and Buhler, D. D. (2002). Utilizing Sorghum as functional model of crop-weed competition. I. Establishing a competitive hierarchy. Weed Science, 50, 466-472. http://dx.doi.org/10.1614/0043-1745(2002)050[0466:USAAFM]2.0.CO;2.
http://dx.doi.org/10.1614/0043-1745(2002...
). Another assumption to demonstrate competitive advantage is that at least 2 indices must have statistical significance (Bianchi et al. 2006Bianchi, M. A., Fleck, N. G. and Lamego, F. P. (2006). Proporção entre plantas de soja e plantas competidoras e as relações de interferência mútua. Ciência Rural, 36, 1380-1387. http://dx.doi.org/10.1590/S0103-84782006000500006.
http://dx.doi.org/10.1590/S0103-84782006...
). According to this, barnyardgrass was more competitive than BMX Apolo RR only regarding plant height.

Table 4
Competitiveness indices between soybean (BMX Apolo RR and BMX Potência RR cultivars) and barnyardgrass (Echinochloa crus-galli) for the variables relative competitiveness, coefficients of relative crowding and of competitiveness obtained in experiments conducted in substitutive series.

Plant height is an important trait for the growth and development of species in the environment because it is directly related to the light capture efficiency (Fleck et al. 2008Fleck, N. G., Agostinetto, D., Galon, L. and Schaedler, C. E. (2008). Competitividade relativa entre cultivares de arroz irrigado e biótipo de arroz‑vermelho. Planta Daninha, 26, 101‑111. http://dx.doi.org/10.1590/S0100-83582008000100011.
http://dx.doi.org/10.1590/S0100-83582008...
). Thus, it is possible that the experimental period (44 DAE) was relatively short to show differences in other variables such as the expansion of leaf area and SDM. However, Saccol and Estefanel (1995)Saccol, A. V. and Estefanel, V. (1995). Competição entre o capim-arroz e a soja cultivada em solo hidromórfico. II. Efeito sobre algumas características agronômicas da soja. Pesquisa Agropecuária Brasileira, 30, 327-338. reported that the competition period of barnyardgrass, from which soybean height and leaf area is significantly reduced, is about 20 to 22 days, respectively.

The competitiveness indices showed greater values for barnyardgrass compared to BMX Apolo RR concerning plant height, probably due to the small height of the cultivar, which facilitated weed growth and greater SDM accumulation. On the other hand, the characteristics of BMX Potência RR contributed to higher relative growth of barnyardgrass, expressed mainly by increasing photosynthetic activity and carbon sequestration efficiency, which directly contributed to increase plant height, leaf area and dry matter accumulation. The BMX Potência RR cultivar has average development cycle (6.7), and, consequently, emergence and growth rates are lower compared to the super-early-cycle cultivars such as BMX Apolo RR (5.8). These features facilitate the growth of weeds, especially barnyardgrass, which has high growth rate at the early developmental stages (Kissmann 2007Kissmann, K. G. (2007). Plantas infestantes e nocivas. Tomo I. 3. ed. São Paulo: Basf Brasileira S. A. CD-ROM.).

A study diagnosed that plant height soybean plants with late cycle have greater competitive ability than early-maturing, shorter cultivars (Lamego et al. 2004Lamego, F. P., Fleck, N. G., Bianchi, M. A. and Schaedler, C. E. (2004). Tolerância à interferência de plantas competidoras e habilidade de supressão por genótipos de soja – II respostas de variáveis de produtividade. Planta Daninha, 22, 491-498. http://dx.doi.org/10.1590/S0100-83582004000400002.
http://dx.doi.org/10.1590/S0100-83582004...
). However, environmental conditions (biotic and abiotic factors) can interfere with the expression of competitive ability in different ways in each cultivar. Therefore, characteristics such as development cycle and plant height may not have much effect on the competitive ability of plants in experiments conducted under controlled conditions, as in a greenhouse (Agostinetto et al. 2008Agostinetto, D., Galon, L., Moraes, P. V. D., Rigoli, R. P., Tironi, S. P. and Panozzo, L. E (2008). Competitividade relativa entre cultivares de arroz irrigado e biótipo de capim-arroz (Echinochloa spp.). Planta Daninha, 26, 757-766. http://dx.doi.org/10.1590/S0100-83582008000400007.
http://dx.doi.org/10.1590/S0100-83582008...
), which is the case in this study.

Based on morphological and physiological variables, it is emphasized that the intraspecific competition was more harmful than interspecific competition for barnyardgrass. This can be explained mainly by the high demand for nitrogen of barnyardgrass (Kissmann 2007Kissmann, K. G. (2007). Plantas infestantes e nocivas. Tomo I. 3. ed. São Paulo: Basf Brasileira S. A. CD-ROM.), so that, in the weed monoculture, intraspecific competition for nitrogen is greater, but without discarding the competition for other environmental resources. In contrast, the highest growth rate of the weed, both in plant height and leaf area production, makes it capture the light more efficiently and, consequently, at more advanced growth stages, it may shade the crop and reduce its growth (Place et al. 2011Place, G. T., Reberg-Horton, S. C., Dickey, D. A. and Carter JR, T. E. (2011). Identifying soybean traits of interest for weed competition. Crop Science, 51, 2642-2654. http://dx.doi.org/10.2135/cropsci2010.11.0654.
http://dx.doi.org/10.2135/cropsci2010.11...
). From this study, it is not possible to conclude that barnyardgrass is more competitive than soybean crop because the main negative effect of the competitor on the cultivars was to decrease plant height, especially for BMX Apolo RR, while all other morphophysiological parameters remained virtually unchanged.

CONCLUSION

The BMX Apolo RR and BMX Potência RR cultivars show equivalent relative competitiveness with barnyardgrass, which exhibits higher competitiveness to the BMX Apolo RR soybean cultivar, regarding to plant height. Intraspecific competition is more important for barnyardgrass since it affected negatively morphophysiological characteristics of the species.

ACKNOWLEDGEMENTS

The authors thank the Coordination for the Improvement of Higher Education Personnel (CAPES), for the master scholarship granted to the first author, as well as the National Council for Scientific and Technological Development (CNPq) and Rio Grande do Sul Research Foundation (FAPERGS) for granting scientific initiation scholarships.

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Publication Dates

  • Publication in this collection
    29 Sept 2016
  • Date of issue
    Oct-Dec 2016

History

  • Received
    02 Sept 2015
  • Accepted
    26 Jan 2016
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