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Resistance of Botrytis cinerea to fungicides controlling gray mold on strawberry in Brazil

ABSTRACT

The aim of this study was to evaluate the resistance of Botrytis cinerea to the fungicides currently used for its control in Brazil. Isolates of the fungus were collected from different strawberry-producing fields in the states of Espírito Santo, Minas Gerais, and São Paulo, Brazil. First, a total of 183 isolates were identified at the species level using specific primers for the glyceraldehyde-3-phosphate dehydrogenase (G3PDH) gene. The isolates were grown on potato dextrose agar (PDA) containing the fungicides procymidone, iprodione, and thiophanate-methyl in different concentrations: 0.0 (control), 0.1; 1.0; 10.0; 100.0 and 1,000.0 μg∙mL−1. The percentage of mycelial growth inhibition was used to determine the effective concentration of the fungicide that was able to inhibit colony growth by 50% (EC50). Approximately 25.7% of the isolates were resistant to iprodione, 53.0% were resistant to procymidone, and 93.0% were resistant to thiophanate-methyl. Moreover, crossresistance and multiple resistance were verified, with 19.7% of the isolates showing resistance to 3 fungicides simultaneously. This finding explains the ineffectiveness of fungicides application to control gray mold in strawberry fields in Brazil and highlights the need for new strategies to manage this disease in the culture.

Key words
Fragaria x ananassa; chemical control; dicarboxamides; methyl benzimidazole carbamate

INTRODUCTION

Gray mold caused by the fungus Botrytis cinerea Pers. ex Fries is a very common disease, affecting approximately 200 species of different botanical families, among them fruits, vegetables, ornamental, and wild plants (Elad et al. 2007Elad, Y., Williamson, B., Tudzynski, P. and Delen, N. (2007). Botrytis: biology, pathology and control. Dordrecht: Kluwer Academic Publishers.; Williamson et al. 2007Williamson, B., Tudzynski, B., Tudzynski, P. and van Kan, J. A. (2007). Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology, 8, 561-580. http://dx.doi.org/10.1111/j.1364-3703.2007.00417.x.
http://dx.doi.org/10.1111/j.1364-3703.20...
). The fungus presents high adaptability, occurring in different areas such as tropical, subtropical, temperate dry and wet, and causes severe damage (Elad et al. 2007Elad, Y., Williamson, B., Tudzynski, P. and Delen, N. (2007). Botrytis: biology, pathology and control. Dordrecht: Kluwer Academic Publishers.).

Gray mold is considered the main disease in strawberry (Fragaria x Ananassa) production (Maas 1998Maas, J. L. (1998). Compendium of strawberry diseases. Saint Paul: American Phytopathological Society Press.; Costa et al. 2014Costa, H., May De Mio, L. L., Ruaro, L., Moreira, L. M. and Ventura, J. A. (2014). Como produzir Morangos. In M. A. C. Zawadneak, J. M. Schuber and A. F. Mógor (Eds.), Manejo integrado de doenças do morangueiro (p. 193-215). Curitiba: Editora da UFPR.) because the fungus can infect leaves, stems, flowers, and fruits at any stage of development. Infected organs become necrotic, with an intense sporulation, and a gray color, which characterizes the disease. Although the fungus can infect all parts of the plant, the damage is more common during the production phase when the pathogen infects flowers (Bristow et al. 1986Bristow, P., McNicol, R. and Williamson, B. (1986). Infection of strawberry flowers by Botrytis cinerea and its relevance to grey mould development. Annals of Applied Biology, 109, 545-554. http://dx.doi.org/10.1111/j.1744-7348.1986.tb03211.x.
http://dx.doi.org/10.1111/j.1744-7348.19...
; Maas 1998Maas, J. L. (1998). Compendium of strawberry diseases. Saint Paul: American Phytopathological Society Press.; Elad et al. 2007Elad, Y., Williamson, B., Tudzynski, P. and Delen, N. (2007). Botrytis: biology, pathology and control. Dordrecht: Kluwer Academic Publishers.; Costa et al. 2014Costa, H., May De Mio, L. L., Ruaro, L., Moreira, L. M. and Ventura, J. A. (2014). Como produzir Morangos. In M. A. C. Zawadneak, J. M. Schuber and A. F. Mógor (Eds.), Manejo integrado de doenças do morangueiro (p. 193-215). Curitiba: Editora da UFPR.).

Chemical control by fungicides is one of the most important measures to control gray mold worldwide. In Brazil, under favorable weather conditions, several applications are required during a culture cycle (Kimati et al. 1997Kimati, H., Amorim, L., Bergamin Filho, A., Camargo, L. and Rezende, J. (1997). Manual de fitopatologia: doenças das plantas cultivadas. São Paulo: Agronômica Ceres.; Costa et al. 2014Costa, H., May De Mio, L. L., Ruaro, L., Moreira, L. M. and Ventura, J. A. (2014). Como produzir Morangos. In M. A. C. Zawadneak, J. M. Schuber and A. F. Mógor (Eds.), Manejo integrado de doenças do morangueiro (p. 193-215). Curitiba: Editora da UFPR.). Currently, only 3 groups of fungicides are registered by the Ministério da Agricultura, Pecuária e Abastecimento (MAPA) for strawberry gray mold control in Brazil: Dicarboxamides, Methyl Benzimidazole Carbamate (MBC), and Anilinopyrimidine (Brasil 2016Brasil (2016). Ministério da Agricultura, Pecuária e Abastecimento; [accessed 2016 Feb 26]. http://www.agricultura.gov.br/
http://www.agricultura.gov.br/...
).

Although chemical control is the main strategy to control gray mold, the emergence of fungal populations resistant to fungicides has been a great challenge for strawberry growers (Brent and Hollomon 2007bBrent, K. J. and Hollomon, D. W. (2007b). Fungicide resistance in crop pathogens: how can it be managed? Brussels: Global Crop Protection Federation.). Due to the loss of efficiency of the fungicides, producers tend to increase their use to achieve an effective control of the disease (Zambolim et al. 2007Zambolim, L., Venâncio, S. and Oliveira, S. (2007). Manejo da resistência de fungos a fungicidas. Visconde do Rio Branco: Suprema Gráfica e Editora.). Several studies have reported cases of resistance of B. cinerea to fungicides of the dicarboxamide and MBC groups. Yourman and Jeffers (1999)Yourman, L. and Jeffers, S. (1999). Resistance to benzimidazole and dicarboximide fungicides in greenhouse isolates of Botrytis cinerea. Plant Disease, 83, 569-575. http://dx.doi.org/10.1094/PDIS.1999.83.6.569.
http://dx.doi.org/10.1094/PDIS.1999.83.6...
investigated the sensitivity of B. cinerea isolates in ornamental plantations and showed that more than 80% of the isolates analyzed were resistant to thiophanate-methyl fungicide. Furthermore, the occurrence of multi-resistance to dicarboxamides and benzimidazoles has been demonstrated. LaMondia and Douglas (1997)LaMondia, J. and Douglas, S. (1997). Sensitivity of Botrytis cinerea from Connecticut greenhouses to benzimidazole and dicarboximide fungicides. Plant Disease, 81, 729-732. http://dx.doi.org/10.1094/PDIS.1997.81.7.729.
http://dx.doi.org/10.1094/PDIS.1997.81.7...
found that 70% of B. cinerea isolates obtained from a greenhouse were resistant to benomyl and thiophanate-methyl fungicides, while approximately 35% of the isolates were resistant to iprodione fungicide. Isolates of B. cinerea obtained from pear also showed resistance to iprodione and thiabendazole fungicides (Lennox and Spotts 2003Lennox, C. L. and Spotts, R. A. (2003). Sensitivity of populations of Botrytis cinerea from pear-related sources to benzimidazole and dicarboximide fungicides. Plant Disease, 87, 645-649. http://dx.doi.org/10.1094/PDIS.2003.87.6.645.
http://dx.doi.org/10.1094/PDIS.2003.87.6...
). Resistance of this fungus to dicarboxamides and MBC fungicides is common, and there are reported cases of isolates resistant to thiophanatemethyl, pyraclostrobin, boscalid, cyprodinil, fenhexamid, iprodione, and fludioxonil fungicides, simultaneously (Fernández-Ortuño et al. 2014Fernández-Ortuño, D., Grabke, A., Bryson, P. K., Amiri, A., Peres, N. A. and Schnabel, G. (2014). Fungicide resistance profiles in Botrytis cinerea from strawberry fields of seven southern U.S. states. Plant Disease, 98, 825-833. http://dx.doi.org/10.1094/PDIS-09-13-0970-RE.
http://dx.doi.org/10.1094/PDIS-09-13-097...
). Although various mechanisms by which B. cinerea becomes resistant to fungicides have been identified, the most common is the emergence of mutations in the target sites of fungicides (Leroux et al. 2002Leroux, P., Fritz, R., Debieu, D., Albertini, C., Lanen, C., Bach, J., Gredt, M. and Chapeland, F. (2002). Mechanisms of resistance to fungicides in field strains of Botrytis cinerea. Pest Management Science, 58, 876-888.).

Understanding the profile of pathogen populations in cultivated areas is one of the first steps for an integrated management of this disease. In Brazil, little is known on the resistance of B. cinerea populations to fungicides registered for strawberry crop. Therefore, the goal of this study was to evaluate the resistance of B. cinerea isolates obtained from different strawberry-producing fields in Brazil to fungicides most widely used for its control. This information is important to help in the development of strategies aiming to improve disease control and prevent the loss of fungicide efficiency.

MATERIAL AND METHODS

Obtaining the isolates

The isolates were obtained from strawberry plants showing symptoms of gray mold in commercial plantations in 18 different cities in the states of Espírito Santo (ES), Minas Gerais (MG), and São Paulo (SP), Brazil, during 2012, totaling 183 isolates (Table 1).

Table 1
Number of isolates collected in different cities in the states of Espírito Santo, Minas Gerais, and São Paulo.

In each field, a sample of leaf, flower or fruit was collected and placed in labeled plastic bags. To obtain monosporic isolates, conidia were collected from the surface of infected material and placed in 1 mL of sterile distilled water containing 1% Tween 60. Approximately 200 µL of conidial suspension were spread in a Petri dish containing water-agar medium and streptomycin (100 µg∙mL−1). After incubation at 22 °C for 24 – 36 h, 1 germinated conidia was collected and transferred to potato dextrose agar (PDA) medium. All isolates were stored in silica and maintained at 5 °C.

Survey concerning the fungicides used to control gray mold of strawberry

During the collection of isolates in the different producing areas, a survey was conducted on fungicide use. This information was obtained from 121 areas of commercial cultivation in the 3 states listed above.

Molecular identification of the isolates

Genomic DNA was extracted from all isolates, quantified using a spectrophotometer, and diluted to a final concentration of 50 ng∙μL−1. Species identification was performed by polymerase chain reaction (PCR) using primers that targeted partial sequences of the glyceraldehyde-3-phosphate dehydrogenase gene (G3PDH), G3PDH-F1 (5’- GGACCCGAGCTAATTTATGTCACGT-3’), and G3PDH-F2 (5’- GGGTGTCAACAACGAGACCTACACT- 3’), specific to B. caroliniana and B. cinerea, respectively. These primers were combined with the reverse primer G3PDH-R (5’-ACCGGTGCTCGATGGGATGAT -3’) (Li et al. 2012aLi, X., Fernández-Ortuño, D., Chai, W., Wang, F. and Schnabel, G. (2012a). Identification and prevalence of Botrytis spp. from blackberry and strawberry fields of the Carolinas. Plant Disease, 96, 1634-1637. http://dx.doi.org/10.1094/PDIS-02-12-0128-RE.
http://dx.doi.org/10.1094/PDIS-02-12-012...
). The amplification conditions were considered according to Li et al. (2012a)Li, X., Fernández-Ortuño, D., Chai, W., Wang, F. and Schnabel, G. (2012a). Identification and prevalence of Botrytis spp. from blackberry and strawberry fields of the Carolinas. Plant Disease, 96, 1634-1637. http://dx.doi.org/10.1094/PDIS-02-12-0128-RE.
http://dx.doi.org/10.1094/PDIS-02-12-012...
. Isolates of B. cinerea (CA25, WM6, and CB17) and B. caroliniana (CA3, WM4, CB15, and BC11HP33) (Li et al. 2012aLi, X., Fernández-Ortuño, D., Chai, W., Wang, F. and Schnabel, G. (2012a). Identification and prevalence of Botrytis spp. from blackberry and strawberry fields of the Carolinas. Plant Disease, 96, 1634-1637. http://dx.doi.org/10.1094/PDIS-02-12-0128-RE.
http://dx.doi.org/10.1094/PDIS-02-12-012...
; Li et al. 2012bLi, X., Kerrigan, J., Chai, W. and Schnabel, G. (2012b). Botrytis caroliniana, a new species isolated from blackberry in South Carolina. Mycology, 104, 650-658. http://dx.doi.org/10.3852/11-218.
http://dx.doi.org/10.3852/11-218...
), which were characterized in previous studies, were used as positive controls.

Sensitivity of the isolates to fungicides

To obtain different concentrations of the active ingredients (a.i.) of the fungicides iprodione, procymidone, and thiophanate-methyl, commercial products were used: Rovral SC (500 g∙L−1 iprodione), Sumilex 500 WP (500 g∙L−1 procymidone), and Cercobin 700 WP (500 g∙L−1 thiophanate-methyl). These fungicides were selected because they are the most widely used in strawberry crops to control gray mold, based on our previous survey (Table 2).

Table 2
Survey concerning the use of fungicides in different areas from where the isolates used in this study were obtained.

Each fungicide was added to PDA medium at concentrations of 0.0 (control), 0.1; 1.0; 10.0; 100.0 and 1,000.0 μg∙mL−1. Then, a 7-mm diameter mycelial plug (obtained from 3-day-old colonies grown on PDA) was placed at the center of Petri dishes containing 8 mL of PDA + fungicide at different concentrations. For each concentration of fungicide, 3 Petri dishes were used, accounting for 3 replicates. The Petri dishes were maintained at 22 °C in the dark. After the second day of growth, the diameter of the colony was measured in 2 perpendicular directions with the aid of a digital caliper (Metrotools - 150 mm). Using the control treatment (0.0 μg∙mL−1 of fungicide) as standard, the percentage of growth at each concentration was obtained. This value was subtracted from 100 to yield the percentage of growth inhibition at each dose of the fungicide. The effective concentration (μg∙mL−1) of the fungicide that was able to inhibit mycelial growth by 50% (EC50) was calculated for each isolate by linear regression of the mycelial growth inhibition versus the log10 transformation of the fungicide concentration using the software SAS 9.0 (SAS Institute Inc, Cary, NC, USA).

Data analysis

To discriminate the isolates that were sensitive or resistant to the fungicides, we considered previous studies in which this relationship had been well defined. For fungicides of the dicarboxamide group (iprodione and procymidone), the discriminatory dose was 1 μg∙mL−1 (LaMondia and Douglas 1997LaMondia, J. and Douglas, S. (1997). Sensitivity of Botrytis cinerea from Connecticut greenhouses to benzimidazole and dicarboximide fungicides. Plant Disease, 81, 729-732. http://dx.doi.org/10.1094/PDIS.1997.81.7.729.
http://dx.doi.org/10.1094/PDIS.1997.81.7...
; Myresiotis et al. 2007Myresiotis, C., Karaoglanidis, G. and Tzavella-Klonari, K. (2007). Resistance of Botrytis cinerea isolates from vegetable crops to anilinopyrimidine, phenylpyrrole, hydroxyanilide, benzimidazole, and dicarboximide fungicides. Plant Disease, 91, 407-413. http://dx.doi.org/10.1094/PDIS-91-4-0407.
http://dx.doi.org/10.1094/PDIS-91-4-0407...
) compared with 10.0 μg∙mL−1 for the MBC fungicide (thiophanate-methyl) (LaMondia and Douglas 1997LaMondia, J. and Douglas, S. (1997). Sensitivity of Botrytis cinerea from Connecticut greenhouses to benzimidazole and dicarboximide fungicides. Plant Disease, 81, 729-732. http://dx.doi.org/10.1094/PDIS.1997.81.7.729.
http://dx.doi.org/10.1094/PDIS.1997.81.7...
; Lennox and Spotts 2003Lennox, C. L. and Spotts, R. A. (2003). Sensitivity of populations of Botrytis cinerea from pear-related sources to benzimidazole and dicarboximide fungicides. Plant Disease, 87, 645-649. http://dx.doi.org/10.1094/PDIS.2003.87.6.645.
http://dx.doi.org/10.1094/PDIS.2003.87.6...
). Then, the isolates were classified as resistant to dicarboxamide fungicides (iprodione and procymidone) if EC50 > 1.0 and resistant to MBC (thiophanate-methyl) when EC50 > 10.0.

A Pearson correlation analysis between the sensitivity to iprodione and procymidone was performed to check the existence of cross-resistance. Similarly, the correlation between the sensitivity to iprodione or procymidone and thiophanate-methyl was evaluated to determine the existence of multi-resistance. These tests were performed at 5% of significance using the software SAS 9.0 (SAS Institute Inc, Cary, NC, USA).

RESULTS AND DISCUSSION

Among the fungicides registered by the MAPA to control gray mold on strawberry in Brazil (Brasil 2016Brasil (2016). Ministério da Agricultura, Pecuária e Abastecimento; [accessed 2016 Feb 26]. http://www.agricultura.gov.br/
http://www.agricultura.gov.br/...
), the most commonly used are those belonging to the chemical group of dicarboxamides (procymidone and iprodione) (Table 2). In our survey, of the 121 areas of commercial cultivation where information on the use of fungicide could be obtained, in 107 areas the application of at least 1 fungicide was confirmed. In 64.0% of these areas, the fungicide procymidone was used, whereas in 36.0% it was used iprodione. Thiophanatemethyl, belonging to the MBC group, was used in 38.0% of the analyzed areas. Moreover, the simultaneous application of 2 or 3 fungicides was commonly observed.

The isolates showed high variability when grown on PDA medium at 22 °C. Due to these morphological variations, we suspected that the isolates would correspond to different species of Botrytis. However, the analysis of the G3PDH gene fragment, by PCR using specific primers, revealed the presence of a fragment of 238 bp for all isolates, which corresponded to B. cinerea.

Most isolates of B. cinerea (74.3%) were sensitive to the iprodione fungicide, and 31.0% had EC50 values < 0.1 μg∙mL−1. The remaining isolates (25.7%) had EC50 values > 1.0 μg∙mL−1 and were resistant to the fungicide, although the EC50 values did not exceed 10.0 μg∙mL−1 (Figure 1). In the case of procymidone, 47.0% of the isolates were sensitive, and 13.1% had EC50 values < 0.1 μg∙mL−1. The remaining isolates (53.0%) were resistant (EC50 > 1.0 μg∙mL−1), and 5.0% had EC50 values that exceeded 1,000 μg∙mL−1. On the other hand, when assessing the sensitivity of the isolates to the fungicide thiophanatemethyl, a very different behavior was observed, i.e. 93.0% of the isolates were resistant (EC50 values > 10.0 μg∙mL−1), and 73.0% had EC50 values greater than 1,000 μg∙mL−1.

Figure 1
Frequency distribution of the effective concentrationvalues that inhibit mycelial growth by 50% (EC50) for the different isolates grown in medium containing iprodione, procymidone or thiophanate-methyl fungicides

Based on the responses of sensitivity and resistance to procymidone, iprodione, and thiophanate-methyl, the isolates were grouped into 8 different phenotypes (Figure 2). Only 4.9% of the isolates were fully sensitive to all 3 fungicides, whereas 19.7% were resistant to all fungicides simultaneously. Regarding the resistance to only 1 fungicide, procymidone was the one with lowest percentage of resistance (1.6%), followed by iprodione (14.8%) and thiophanate-methyl (22.4%). Multiple resistance (between different chemical groups) to thiophanate-methyl and procymidone was observed in 17.5% of the isolates, and, to thiophanate-methyl and iprodione, in 14.8% of the isolates. The cross-resistance between iprodione and procymidone was observed in 4.4% of the isolates. Positive and significant correlations (p < 0.05) were observed between the resistance to procymidone and iprodione (r = 0.278; p-value = 0.000140) and between thiophanate-methyl and iprodione (r = 0.169; p-value = 0.0220). On the other hand, there was no correlation between the resistance to thiophanate-methyl and procymidone (r = 0.0997; p-value = 0.179).

Figure 2
Number of isolates showing the phenotypes that aresensitive and resistant to iprodione, procymidone, and thiophanatemethyl fungicides.

In ES, most isolates were resistant to thiophanatemethyl (81.3%) and procymidone (68.8%), while only 25.0% of the isolates were resistant to iprodione (Table 3).

Table 3
Percentage of isolates from the states of Espírito Santo, Minas Gerais, and São Paulo showing resistance to the fungicides thiophanate-methyl, procymidone, and iprodione.

The analysis of the isolates from MG showed that 93.7% were resistant to thiophanate-methyl, 49.0% were resistant to procymidone, and 27.2% were resistant to iprodione. All isolates from SP were resistant to thiophanate-methyl and sensitive to iprodione, while 89.0% were resistant to procymidone. In general, the lowest percentage of resistance was observed for the iprodione fungicide, particularly in SP, where resistant isolates were not detected. Therefore, its use to control gray mold on strawberry crops should be prioritized over the other fungicides, especially thiophanate-methyl.

Botrytis cinerea belongs to a group referred to as “high risk” for the development of fungicide resistance due to its high reproductive capacity, facility of dispersion, and wide host range (Brent and Hollomon 2007aBrent, K. J. and Hollomon, D. W. (2007a). Fungicide resistance: the assessment of risk. Brussels: Global Crop Protection Federation.). Additionally, this fungus presents a high genetic variability that is maximized by the presence of transposable elements in the genome (Samuel et al. 2012Samuel, S., Veloukas, T., Papavasileiou, A. and Karaoglanidis, G. S. (2012). Differences in frequency of transposable elements presence in Botrytis cinerea populations from several hosts in Greece. Plant Disease, 96, 1286-1290. http://dx.doi.org/10.1094/PDIS-01-12-0103-RE.
http://dx.doi.org/10.1094/PDIS-01-12-010...
). Moreover, the use of large amounts of fungicide in strawberry plantations has contributed to increase the occurrence of resistance of B. cinerea to fungicides. In this study, it was observed a high resistance of B. cinerea population to iprodione, procymidone, and thiophanate-methyl, which are the fungicides most commonly used in strawberry fields in Brazil.

Despite the lower use of thiophanate-methyl in the analyzed areas compared with dicarboxamides, a high percentage of resistance to thiophanate-methyl was observed in the current study (93.0% of the isolates). It was also verified by Yourman and Jeffers (1999)Yourman, L. and Jeffers, S. (1999). Resistance to benzimidazole and dicarboximide fungicides in greenhouse isolates of Botrytis cinerea. Plant Disease, 83, 569-575. http://dx.doi.org/10.1094/PDIS.1999.83.6.569.
http://dx.doi.org/10.1094/PDIS.1999.83.6...
, who reported that more than 80.0% of the isolates were resistant to this fungicide. The resistance of B. cinerea to fungicides of the MBC group is very common, as observed in several studies (Moorman and Lease 1992Moorman, G. and Lease, R. (1992). Benzimidazole and dicarboximide resistant Botrytis cinerea from Pennsylvania greenhouses. Plant Disease, 76, 477-480. http://dx.doi.org/10.1094/PD-76-0477.
http://dx.doi.org/10.1094/PD-76-0477...
; LaMondia and Douglas 1997LaMondia, J. and Douglas, S. (1997). Sensitivity of Botrytis cinerea from Connecticut greenhouses to benzimidazole and dicarboximide fungicides. Plant Disease, 81, 729-732. http://dx.doi.org/10.1094/PDIS.1997.81.7.729.
http://dx.doi.org/10.1094/PDIS.1997.81.7...
; Lennox and Spotts 2003Lennox, C. L. and Spotts, R. A. (2003). Sensitivity of populations of Botrytis cinerea from pear-related sources to benzimidazole and dicarboximide fungicides. Plant Disease, 87, 645-649. http://dx.doi.org/10.1094/PDIS.2003.87.6.645.
http://dx.doi.org/10.1094/PDIS.2003.87.6...
; Myresiotis et al. 2007Myresiotis, C., Karaoglanidis, G. and Tzavella-Klonari, K. (2007). Resistance of Botrytis cinerea isolates from vegetable crops to anilinopyrimidine, phenylpyrrole, hydroxyanilide, benzimidazole, and dicarboximide fungicides. Plant Disease, 91, 407-413. http://dx.doi.org/10.1094/PDIS-91-4-0407.
http://dx.doi.org/10.1094/PDIS-91-4-0407...
; Yoon et al. 2008Yoon, C. S., Ju, E. H., Yeoung, Y. R. and Kim, BS (2008). Survey of fungicide resistance for chemical control of Botrytis cinerea on Paprika. Plant Pathology Journal, 24, 447-452. http://dx.doi.org/10.5423/PPJ.2008.24.4.447.
http://dx.doi.org/10.5423/PPJ.2008.24.4....
; Fernández-Ortuño et al. 2014Fernández-Ortuño, D., Grabke, A., Bryson, P. K., Amiri, A., Peres, N. A. and Schnabel, G. (2014). Fungicide resistance profiles in Botrytis cinerea from strawberry fields of seven southern U.S. states. Plant Disease, 98, 825-833. http://dx.doi.org/10.1094/PDIS-09-13-0970-RE.
http://dx.doi.org/10.1094/PDIS-09-13-097...
). This resistance has been shown to persist in the population for many years even after the discontinued use of the fungicides (Walker et al. 2013Walker, A. S., Micoud, A., Rémuson, F., Grosman, J., Gredt, M. and Leroux, P. (2013). French vineyards provide information that opens ways for effective resistance management of Botrytis cinerea (grey mould). Pest Management Science, 69, 667-678. http://dx.doi.org/10.1002/ps.3506.
http://dx.doi.org/10.1002/ps.3506...
).

The analyzed population was more sensitive to iprodione and procymidone compared with thiophanatemethyl. Despite this, there were isolates with resistance to iprodione and procymidone, including cases of crossresistance between both fungicides. Grabke et al. (2014)Grabke, A., Fernández-Ortuño, D., Amiri, A., Li, X., Peres, N. A., Smith, P. and Schnabel, G. (2014). Characterization of iprodione resistance in Botrytis cinerea from strawberry and blackberry. Phytopathology, 104, 396-402. http://dx.doi.org/10.1094/PHYTO-06-13-0156-R.
http://dx.doi.org/10.1094/PHYTO-06-13-01...
analyzed B. cinerea populations in strawberry plantations located in Florida, North Carolina, and South Carolina and also observed isolates resistant to these fungicides. LaMondia and Douglas (1997)LaMondia, J. and Douglas, S. (1997). Sensitivity of Botrytis cinerea from Connecticut greenhouses to benzimidazole and dicarboximide fungicides. Plant Disease, 81, 729-732. http://dx.doi.org/10.1094/PDIS.1997.81.7.729.
http://dx.doi.org/10.1094/PDIS.1997.81.7...
studied the resistance of B. cinerea to MBC (benomyl and thiophanate-methyl) and dicarboxamide (iprodione) and observed that 70.0% of the isolates were resistant to the fungicides of the first group and approximately 35.0% were resistant to the second group. In addition, there were cases of crossresistance, which is similar to our findings.

Multiple resistance to the fungicides of the dicarboxamide and MBC groups was observed in the analyzed population, with 17.5% of the isolates resistant to thiophanate-methyl and procymidone, 14.8% resistant to thiophanate-methyl and iprodione, and 19.7% resistant to the 3 fungicides simultaneously. Although these data are alarming, they are consistent with other studies that have reported the simultaneous resistance of B. cinerea isolates to thiophanate-methyl, pyraclostrobin, boscalid, cyprodinil, fenhexamid, iprodione, and fludioxonil fungicides (Amiri et al. 2013Amiri, A., Heath, S. and Peres, N. (2013). Phenotypic characterization of multifungicide resistance in Botrytis cinerea isolates from strawberry fields in Florida. Plant Disease, 97, 393-401. http://dx.doi.org/10.1094/PDIS-08-12-0748-RE.
http://dx.doi.org/10.1094/PDIS-08-12-074...
; Fernández-Ortuño et al. 2014Fernández-Ortuño, D., Grabke, A., Bryson, P. K., Amiri, A., Peres, N. A. and Schnabel, G. (2014). Fungicide resistance profiles in Botrytis cinerea from strawberry fields of seven southern U.S. states. Plant Disease, 98, 825-833. http://dx.doi.org/10.1094/PDIS-09-13-0970-RE.
http://dx.doi.org/10.1094/PDIS-09-13-097...
).

Our research has raised a serious concern in strawberry crop production in Brazil regarding the resistance of B. cinerea to the 3 fungicides most widely used to control gray mold. The high levels of resistance observed with thiophanate-methyl and the high number of isolates with resistance to the 3 fungicides are alarming data. Taken together, the information generated from this study will be helpful for the development of strategies aiming to improve disease control and will lead to an efficient and sustainable use of fungicides in strawberry culture.

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Data availability

Data citations

Brasil (2016). Ministério da Agricultura, Pecuária e Abastecimento; [accessed 2016 Feb 26]. http://www.agricultura.gov.br/

Publication Dates

  • Publication in this collection
    15 May 2017
  • Date of issue
    Apr-Jun 2017

History

  • Received
    27 Feb 2016
  • Accepted
    05 Sept 2016
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