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Jornal de Pediatria

Print version ISSN 0021-7557

J. Pediatr. (Rio J.) vol.81 no.1 Porto Alegre Jan./Feb. 2005 



Bronchial provocation tests using methacholine, cycle ergometer exercise and free running in children with intermittent asthma


Ana C. T. G. SouzaI; Carlos A. C. PereiraII

IPreceptor of Pediatric Pulmonology, Hospital do Servidor Público Estadual Francisco Morato de Oliveira (HSPE-FMO), São Paulo, SP, Brazil
IIPh.D., School of Medicine, Universidade Federal de São Paulo (UNIFESP-EPM). Director of the Laboratory of Lung Functioning, Hospital do Servidor Público Estadual Francisco Morato de Oliveira (HSPE-FMO), São Paulo, SP, Brazil





OBJECTIVE:To compare airway responsiveness to methacholine, cycle ergometer exercise and free running in children with intermittent asthma.
METHODS: A randomized study was conducted with 30 children of both genders with intermittent asthma. Each child was submitted to challenge testing on three separate days, in random order: a) Methacholine challenge using a dosimeter; b) Exercise challenge testing — free running along a 50-meter-long corridor; c) Dry-air exercise challenge on a cycle ergometer. Target heart rate during exercise was 80 to 90% of the maximum predicted value. Spirometry was performed 3, 6,10,15,20 and 30 minutes after exercise. Exercise-induced bronchospasm was defined as a decrease in FEV1 of >10% in comparison to pretest values.
RESULTS: Mean age was 11±3 years. FEV1 and FEV1/FVC ratios were normal and similar before all provocation tests. The maximum heart rate was 178±7 bpm during cycling and 181±6 bpm during the free running test (p > 0.05). Significant bronchospasm was observed with methacholine in 23 cases, after free running in 19 and after cycling in 14 children (p < 0.05, methacholine vs exercise tests). Taking all FEV1 measurements after exercise into account, the free running test resulted in greater exercise-induced bronchospasm in comparison with exercise made on a cycle ergometer (p = 0.003, c2 = 8.948). There was a significant, but poor, correlation between the maximum percentage decrease in FEV1 after free running and cycling (r = 0.46, p < 0.01).
CONCLUSIONS: Methacholine challenge identifies a greater number of asthmatics in comparison to exercise tests. When bronchial provocation test with methacholine is not available, free running should be the test of choice due to its simplicity and greater ability to induce bronchospasm.

Key words: Asthma, bronchial provocation tests, methacholine chloride, exercise test, exercise-induced asthma.




Asthma is a multifactorial disease determined by the interaction of genetic and environmental factors, which result in chronic airway inflammation.

There is no "gold standard" for the diagnosis of asthma1, and the disease often is diagnosed by the combination of detailed clinical history, presence of totally or partially reversible bronchial obstruction after the use of a bronchodilator and diurnal variation of airway obstruction. However, the symptoms of asthma are non-specific and may occur in isolation. This is especially observed in children, in whom recurrent coughing, mainly during the night or after exercise, may be the only clinical manifestation of asthma. The objective measures of bronchial hyperresponsiveness are used in cases of diagnostic suspicion, when there are respiratory symptoms that may indicate asthma, in case of normal spirometric findings.

The correlation between asthma and bronchial responsiveness is complex and controversial, though,2,3 since several studies have shown that normal individuals who have positive bronchial provocation test results with bronchoconstrictive and asthmatic agents may be non-responsive sometimes.4

Bronchial provocation challenge by exercise has been proposed for the diagnosis of asthma.5 This test is usually more specific and can distinguish asthma from other chronic lung diseases,6,7 but it is less sensitive than bronchial provocation with methacholine or histamine in diagnosing asthma. Some authors also observed that bronchial provocation challenge by exercise and methacholine or histamine does not always identify the same individuals.1,8

Different exercise modalities seem to result in different degrees of bronchospasm. Some studies found the following decreasing order in exercise-induced bronchospasm (EIB), when a similar exercise load was used: free running > treadmill running > cycle ergometer > swimming.5 Other authors noted that when the exercise is performed in an environment where humidity and temperature are controlled, it is the attained ventilation that determines the degree of bronchospasm, regardless of the exercise type.9 Therefore, there seems to be no consensus about this aspect of EIB.

The aim of the present study was to compare two exercise challenge tests, by using different methodologies (dry-air exercise challenge on a cycle ergometer and free running in room air), with the bronchial provocation test with methacholine in the diagnosis of asthma in children with intermittent asthma. The hypothesis was that a standardized exercise challenge test could be an alternative to the bronchial provocation test with methacholine, when the latter is not available. The two exercise tests were also compared to verify whether there is some difference between them concerning the diagnosis of asthma and the respective correlation with bronchial provocation with methacholine.


Patients and methods

Forty-three children (6 to 15 years old) diagnosed with intermittent asthma, treated at the outpatient clinic of pediatric pulmonology of Hospital do Servidor Público Estadual Francisco Morato de Oliveira, were selected. Thirteen patients did not complete the study for different reasons: they could not accomplish all the tests, they had respiratory infection, they missed their follow-up appointments because they changed address (moved to another state), climatic conditions were inappropriate, or they did not show up on the scheduled day for the second exercise test.

The sample size was calculated by binomial proportion, considering an 80% positivity rate for bronchial provocation with methacholine10,11 and a 60% positivity rate for the EIB test12 in patients with intermittent asthma, with a = 0.05 and ß = 0.20.13

The diagnosis of asthma was established according to clinical criteria including a history of at least two episodes of dyspnea and/or wheezing relieved with the use of bronchodilators (after other diagnoses were ruled out). Asthma was classified as intermittent according to the Global Initiative for Asthma (NIH)14 after following up patients for at least three months at the outpatient clinic of pediatric pulmonology. None of the patients was receiving oral or inhaled corticosteroid, disodium cromoglycate, nedocromil, theophylline, antileukotrienes and long-acting beta-2 agonists. No children had presented with clinically apparent upper airway infection or exacerbation of asthma in the last six weeks prior to the study.

All patients were asked not to drink coffee, tea or soft drinks containing caffeine two hours before the test, to discontinue the use of short-acting bronchodilators 12 hours before and also to discontinue the use of short- and long-acting antihistamines 48 hours and five days before the tests, respectively.

The study was carried out at the Pulmonary Function Laboratory of Hospital do Servidor Público Estadual. The tests were always performed in the morning (8-10 a.m.), at an average temperature of 21 and 22 ºC and relative humidity around 50% in order to standardize the factors that influence EIB.

At baseline spirometry, all patients had forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV1) greater than or equal to 80% and FEV1/FVC greater than 75% of the predicted value, and were then submitted to the bronchial provocation test with methacholine. The order of the tests (cycle ergometer exercise and free running) was randomly chosen on the computer. There was a minimum interval of 24 hours and a maximum interval of 10 days between each of the three tests.

All parents or surrogates signed an informed consent form allowing their children to participate in the study. The study protocol was approved by the local Ethics and Research Committee.


Spirometry was performed by a laboratory technician, certified by the SBPT (Brazilian Society of Pulmonology and Phthisiology), using an Anamed spirometer, model AM4000 (Brazil), according to the ATS (American Thoracic Society).15 The predicted FEV1 values were based on the data described by Polgar & Promadhat.16 Noseclips were used.

Bronchial provocation with methacholine

Bronchial responsiveness with methacholine chloride was measured using a dosimeter (Multispiro, Incorporated Multichalenge Nebulizer System, USA), coupled to a Pulmo-Aid compressor, DeVilbiss Co. (Somerset, PA, USA). Two milliliters of the solution (concentrations of 0.25; 1.25; 2.5; 5; 10 and 25 mg/ml) was added to the nebulizer and an aerosol was produced by the compressor at a flow rate and pressure of 20 psi, appropriate for an output of 0.045 ml/discharge (total of 0.225 ml with five discharges). The output was regulated by a solenoid valve that was activated during inspiration and remained open for 0.6 seconds. The patients wore noseclips and were told to take five deep breaths going from functional residual capacity (FRC) to total lung capacity (TLC) and then to hold their breath for 2 to 5 seconds at TLC in order to maximize pulmonary deposition. This was repeated five times in a row for each methacholine concentration. The doses were administered at five-minute intervals and FEV1 was measured one minute after the last inhalation.

The test result was expressed by the cumulative dose that caused a 20% decrease in FEV1 (PD20 FEV1%) obtained by linear interpolation around the desired cutoff point of the fall in FEV1 values. The cutoff dose was 6.6 µmol,17 considering that:

- Increased responsiveness: less than or equal to 0.125 µmol;
- moderate responsiveness: between 0.125 and 1.99 µmol;
- mild responsiveness: between 2.00 and 6.6 µmol.

Free running

After having their baseline lung function measured, the children ran along a 50-meter corridor, breathing in the ambient air. The children were encouraged to run for 1 to 2 minutes until they reached the target submaximal heart rate (HR), around 80 to 90% of the maximum HR (220- age). From this point, HR was maintained for six minutes. HR was controlled by a pulse oximeter (Dixtal), and recorded every minute. The criteria for plethysmographic wave evaluation were met on all tests. Noseclips were used to induce mouth breathing during the tests.

Cycle ergometer exercise

The children exercised on a cycle ergometer (Godart, Netherlands). The workload was adjusted in order to reach a heart rate between 80 and 90% of the maximum HR after a warm-up period of 1 to 2 minutes, using the following equation: workload = (53.76 x measured FEV1) — 11.07.

The workload was adjusted to 60% in the first minute, 75% in the second minute and 90 to 100% for the remaining time (6 minutes). HR was monitored every minute to determine whether submaximal HR was being reached. During this test, the children inhaled dry air through a mouthpiece coupled to a Hans Rudolph one-way valve. The dry air was produced by a compressed air cylinder and collected in a Douglas bag. HR was also measured throughout the exercise using a pulse oximeter (Dixtal). The temperature of the dry air leaving the Douglas bag was measured throughout the test. Noseclips were also used for the test.

After each exercise test, spirometry was performed again at 3, 6, 10, 15, 20, 30 minutes after the exercise by a laboratory technician, certified by the SBPT. The highest value of the three FEV1 measurements was considered to represent each interval. The intensity of the maximum response was assessed by the largest decrease in FEV1 after the exercise, expressed as percentage of the baseline FEV1. A decrease in FEV1 of at least 10% after the exercise (decrease in baseline FEV1/FEV1 x 100) was considered as a positive response, since this value represents twice the coefficient of variation for the FEV1 measurement. FEV1 was used to assess the response to exercise because it has less intrasubject variability than the peak expiratory flow (PEF).

After the tests, those patients with an FEV1 less than 90% of the baseline value received a bronchodilator (salbutamol spray, 200 mcg). The patients were only allowed to leave if their FEV1 was greater than 90% of the baseline value, after one or more applications of salbutamol.

Statistical analysis

All statistical tests were made using SPSS version 11 ( General data were expressed as mean±standard deviation. The comparison of continuous data between the three groups was made by repeated measures or paired t test ANOVA. The analysis of specific differences between the different time periods was made using Tukey's test. The correlation between the variables for continuous and non-continuous distribution data was made using Pearson's correlation coefficient and Spearman's rank correlation test, respectively. The concordance between the qualitative results of the tests was established by using the McNemar test, and the association between variables was made by the chi-square test (c2).

An alpha value of 0.05 was established for the rejection of the null hypothesis.



The three bronchial provocation tests (methacholine, cycle ergometer and free running) were well accepted and easily performed by the children. All of the children reached the target heart rate proposed by the exercise tests.

Three (10%) children were asymptomatic in the last year and showed a negative result on the bronchial provocation test with methacholine ("asthma in remission"). However, they had a mild wheezing episode after 45 days, 3 and 4 months after the study, and were therefore included in the analysis.

The patients' general characteristics and the mean baseline FEV1 (absolute and % of the predicted value), FVC (absolute and % of the predicted value) and FEV1 / FVC are shown in Table 1. Sex distribution was similar and the spirometric results were as predicted.



Pulmonary function baseline values before the three bronchial provocation tests were similar on each of the days (FEV1, % of the predicted value, methacholine = 101±12, free running = 102±12 and cycle ergometer = 102±12, FVC % of the predicted value, methacholine = 102±11, free running = 101±12 and cycle ergometer = 102±10 and FEV1/FVC methacholine = 86±5, free running = 88±5 and cycle ergometer = 87±4, analysis of variance — ANOVA, FEV1 F = 0.173, p = 0.841 and FVC F = 0,008 and p = 0,99). Temperature and heart rate values were similar on the two days of exercise tests (cycle ergometer = 21±1 oC and free running = 22±2 ºC; initial heart rate, cycle ergometer = 87±10 bpm and free running = 86±11 bpm; target heart rate, cycle ergometer = 178±7 bpm and free running = 181±6 bpm, paired t test = NS).

The frequency of positive results on each test was as follows: methacholine = 6.6 mmol in 23 (77%, 95%CI between 62 and 92%), decrease in FEV1 = 10% in free running and on the cycle ergometer in 19 (63%, 95%CI 45-81%) and 14 children (47%, 95%CI between 29-58%), respectively.

Figure 1 shows the changes in FEV1 values (% of the baseline value) after the cycle ergometer exercise and free running, respectively, according to the duration of exercise. The greater decreases occurred on the free running test (p = 0.003, c2 = 8.948, Friedman's test). For most children, the highest average decrease in FEV1 (% of the baseline value) after bronchial provocation with cycle ergometer exercise occurred at 6 minutes (statistically significant in relation to the baseline value, p = 0.007, analysis of variance followed by Tukey's test) and after free running at 3 minutes (statistically significant in relation to the baseline value, p = 0.001, analysis of variance followed by Tukey's test).



With regard to PD20 for methacholine, the patients responded as follows: two (7%) showed increased hyperresponsiveness, 16 (53%) had moderate hyperresponsiveness, five (17%) demonstrated mild hyperresponsiveness and seven (23%) showed no response. The median PD20 for methacholine was 1.28 µmol. The largest decreases in FEV1 on the ergometer and on the free running test were 52 and 53%, respectively. The highest average decrease in FEV1 (in % of the baseline value) was 13.6 for the ergometer exercise and 18.5% for free running (p = 0.01).

Figure 2 shows a significant but poor correlation between the percentage values of maximum decrease in FEV1 on the ergometer and in free running (r = 0.46, p < 0.01, 95%CI 0.11-0.66). Concordance was noted in only 13 cases, and the two tests yielded positive results in six and negative results in seven individuals.



By analyzing the concordance between the decrease in FEV1 greater than or equal to 10% in the ergometer exercise and the positive or negative response to the methacholine challenge test, it was observed that 18 tests were concordant and that 12 were discordant, and among the 12 discordant tests, 11 were positive only for methacholine (p = 0.006, McNemar test). By performing the same analysis on the free running test, it was observed that 24 tests were concordant with methacholine and that six were discordant. Of the discordant tests, all were positive only for methacholine (p = 0.002, McNemar test).

There was no significant correlation between the percentage of maximum FEV1 decrease on the ergometer and the PD20 for methacholine (rho = 0.311, p = 0.094) nor between the maximum decrease in free running and the PD20 for methacholine (rho = 0.086, p = 0.652).

Figure 3 shows the response to exercise of all study participants, according to the time of FEV1 decrease and the type of exercise. Free running showed a larger number of patients with a positive response to the exercise test (FEV1 decrease > 10%) and also with an FEV1 greater than 40%.




In this study, we compared two types of exercises (cycle ergometer and free running) with bronchial provocation with methacholine, using exercise as an alternative to the pharmacological challenge test for the diagnosis of asthma. There was a positivity of 77% for methacholine, 63% for free running and 47% for the cycle ergometer.

Dry air was used in the ergometer exercise in order to optimize the bronchoconstrictive response, whereas only noseclips were used for the free running test to force mouth breathing, and even so, the highest positivity was that obtained from the free running test. We did not use an air-conditioned laboratory for rigid temperature and humidity control, but both tests were performed within a maximum period of 10 days in order to avoid great climatic changes, and whenever this was the case, the tests were rescheduled (with a maximum of 10 days between the tests). Due to these criteria, three patients were excluded from the study. Relative humidity has more influence than temperature on the production of EIB,18 and for this reason, the tests were not performed during the summertime. Air humidity greater than 50% would not be enough to produce bronchospasm in susceptible individuals.

Only heart rate was used to measure the intensity of the exercise. During exercise, cardiac output increases in proportion to oxygen consumption. Cardiac output at a given submaximal oxygen consumption (80 to 90% of maximum HR) is similar in free running and on the cycle ergometer.19 Nevertheless, the use of heart rate to determine the intensity of exercises is somewhat more variable than the direct measurement of oxygen consumption.19 Perhaps, if oxygen consumption and ventilation were directly measured, the difference between the tests could be better understood.

Bronchial hyperresponsiveness (BHR)

BHR is often measured by the bronchial provocation test with histamine or methacholine; however, bronchial response to exercise can also be used. The use of exercise as objective measure of bronchial hyperresponsiveness in asthma is quite interesting since it is a physiological stimulus, easy to use and widely accepted by children, as observed in this study.

Epidemiological studies show BHR in only 40 to 60% of asthmatic patients. This is partly due to the absence of recent symptoms when the test was performed, but also to other factors such as: bronchial responsiveness has a unimodal distribution in the population (overlapping of healthy and asthmatic individuals), and the distinction between asthmatic and healthy subjects is arbitrary; individual variability of bronchial responsiveness over time; change in airway responsiveness with age, which is larger in children, especially among atopic ones.1,2,4 BHR also is found among smokers, former asthmatics, nonasthmatic patients with allergic rhinitis and even in atopic individuals without any clinical manifestations associated.10 Therefore, depending on clinical and epidemiological circumstances, the presence of BHR is not synonymous with asthma, nor does its absence rule out this diagnosis.

Clinical trials demonstrate that patients with intermittent asthma have BHR around 80%.10,11 In the epidemiological case, if the pretest probability is low (a 5% asthma prevalence), the post-test probability is also low. At the pulmonary function laboratory, where the pretest probability is 40% or more, the post-test probability exceeds 80%. When analyzing a diagnostic test, one should not consider only sensitivity and specificity. The prevalence of the disease is also essential to assess the results.10

The present study revealed that 77% of the children with intermittent asthma responded to the bronchial provocation test with methacholine. Although this value is slightly lower than those observed in previous studies about intermittent asthma, one should not forget that, in this study, three patients were asymptomatic in the last 12 months, which might have contributed to a lower percentage of positive tests. Moreover, patients with intermittent asthma may have bronchodilation when submitted to bronchial provocation tests because deep inspiration may eliminate bronchospasm, resulting in false-negative tests.20 These cases were included so that the relative sensitivity of different tests could be compared in a group of children with intermittent asthma and with recent remission.


Bronchial provocation challenge by exercise is indicated for individuals with history of activity limitations due to bronchospasm, and is used to assess the response to EIB-specific medications and also to diagnose asthma.5 According to several studies, 40 to 90% of asthmatic patients respond abnormally to exercise,1,2,9,21,22 and just as occurs with bronchial provocation with methacholine or histamine, the sensitivity of the test is higher in non-epidemiological studies.

However, the specificity (healthy individuals with negative result/total of healthy individuals) of exercise tests is high (around 90%).17,22 In epidemiological studies, the predictive value of the exercise test (the probability of an individual with a positive result to have asthma) is low,2 since some nonasthmatics respond to exercise and in the general population there are far more nonasthmatics than asthmatics. This large variability between clinical and population-based studies is also due to the selection of patients, different protocols for exercise tests, definition of what is considered normal and an asthmatic patient, and different response criteria.9,17

Several cutoff points have been proposed regarding the percentage decrease in FEV1 on the exercise test 17,22 in order to characterize a test as positive; however, the choice is arbitrary, as the distribution of the decrease is unimodal. In the present study, values of 10% or more for FEV1 decrease were significant, since patients with a previously established diagnosis were included. Less stringent positivity criteria should be chosen when the pretest probability of the disease is high; when such probability is low, more stringent criteria should be used, thus minimizing the proportion of false-positive results.17 The benefit/risk ratio of the treatment should also be considered: relatively less stringent criteria are used when the expected benefits of the treatment are substantial; stringent criteria should be applied when this ratio is low.10

Therefore, whereas the exercise test has a relatively poor role in population-based studies in assessing the incidence of asthma, it is quite useful in clinical trials and in distinguishing asthma from other chronic lung diseases, including those with bronchial hyperresponsiveness to methacholine.6,7

In the present study, the positivity of the ergometer exercise was slightly lower than that described in patients with intermittent asthma, which corresponds to approximately 60%.12 However, it should be underscored that not all patients were responsive to bronchoconstriction with methacholine, which reduces the response to the test.

Exercise versus methacholine

In the available literature, no consensus exists about the correlation of the intensity of bronchospasm observed after bronchial provocation with drugs and exercise.12,22,23 The intensity of exercise-induced bronchoconstriction did not show a significant correlation with bronchial provocation after the inhalation of methacholine (ergometer: rho = 0.311, p = 0.094 and free running: rho = 0.086, p = 0.652).

In some patients, there was some discrepancy between the methacholine challenge and free running tests and between the methacholine and cycle ergometer tests, thus corroborating the results of clinical trials in which bronchial provocation tests with histamine or methacholine and exercise probably measure different aspects of bronchial hyperresponsiveness.1,22 By comparing the bronchial provocation tests, methacholine was statistically better than both exercise tests because it detected the largest number of patients with asthma (methacholine versus ergometer: p = 0.006; methacholine versus free running: p = 0.002). We also observed that children with PD20 greater than 3.74 µmol did not show a decrease in FEV1 after the exercise greater than 20%. The superiority of bronchial provocation tests with methacholine or histamine compared to exercise tests in the diagnosis of asthma also was observed by other authors.2

Free running versus cycle ergometer exercise

Under carefully controlled and standardized conditions, free running produced more EIB than did the exercise on the cycle ergometer (63% in free running and 47% on the cycle ergometer); furthermore, there were significantly larger FEV1 decreases in free running compared to the exercise on the cycle ergometer, even when cold air inhalation was used for the ergometer test, between 3 and 15 minutes of exercise. Also, at all times, free running had the largest number of individuals with an FEV1 decrease greater than or equal to 10%. In addition, severe EIB (> 40%) also was found more often in the free running exercise.

Several factors influence the development and intensity of EIB, such as: type of physical activity, environmental conditions, intensity and duration of the exercise (oxygen consumption and ventilation).5,9,18 According to Otani et al., the intensity of the response to the exercise is directly related to airway vascular hyperpermeability, eosinophilic inflammation and bronchial hyperreactivity.24

In an old but classic review of the literature5 on exercise-induced asthma, the authors demonstrated that free running had a more asthmogenic effect, disregarding environmental factors such as relative humidity and temperature. Other studies with similar workloads (direct measurement of oxygen consumption) showed contradictory results.25,26

It is likely, however, that the type of exercise itself may have an independent effect on the bronchoconstrictive response to exercise. Bar-Yishay et al.27 compared children who practiced free running and swimming with inhalation of dry air under the same ventilation and oxygen consumption conditions and noted that free running produced EIB more frequently.

Other environmental factors (e.g.: exposure to pollutants) should also be taken into account. The practice of exercise in environments with high concentrations of ozone associated with the use of noseclips (mouth breathing) reduces the removal of this gas from the inspired air, leading to a substantially higher intrathoracic deposition and potentially producing remarkable bronchospasm in the free running test.28

With regard to ventilation, the studies yielded inconsistent results. Some authors observed higher ventilation in the cycle ergometer exercise,29-31 whereas others32 found variable results depending on the analyzed individuals, controls or athletes. However, in these two groups, during maximum exercise, ventilation was significantly higher in the treadmill exercise. Gavin & Stager33 showed that ventilation may probably vary according to the type of exercise, but differences in oxygen saturation between the cycle ergometer exercise and free running cannot be explained by the changes in ventilation parameters.

West et al.,21 in their epidemiological study, concluded that the use of a cycle ergometer with dry air inhalation is a sophisticated method that offers little advantage over free running. These authors suggest the standardization of minute ventilation in future studies.

The pathophysiological mechanisms involved in the discrepancy between the two exercise tests have not been properly clarified yet. New studies should be performed comparing the different types of exercises with ventilatory measurements in a larger number of cases.

We conclude that bronchial provocation with methacholine detects a larger number of asthmatic individuals than the two exercise tests, and is therefore the method of choice for the diagnosis of asthma. When bronchial provocation with methacholine or similar drug is not available, a standardized exercise test may be an alternative, since 82% of the patients with a positive result on the methacholine test showed a significant decrease on the free running test. For the study of exercise-induced asthma, the free running test, a simple and practical method, is better than the cycle ergometer exercise, as it identifies a larger number of asthmatic individuals and produces significantly larger decreases in FEV1.



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Correspondence to
Ana Clara Toschi Gianotti de Souza
Rua Ibaragui Nissui, 138
CEP 04116-200 — São Paulo, SP - Brazil
Phone: +55 (11) 5571.5278
Fax: +55 (11) 287.0860

Manuscript received Mar 23 2004, accepted for publication Oct 27 2004.