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J. Pediatr. (Rio J.) vol.88 no.1 Porto Alegre Jan./Feb. 2012
Carine Danielle Ferreira Costa LeiteI; Gabriela Câmara VicenteII; Akemi SuzukiII; Aline D'Avila PereiraII; Gilson Teles BoaventuraIII; Ronald Marques dos SantosIV; Luis Guillermo Coca VelardeV
IMestranda, Ciências Médicas, Universidade
Federal Fluminense (UFF), Niterói, RJ, Brazil
IIEstudante de Graduação, Nutrição, UFF, Niterói, RJ, Brazil
IIIProfessor associado, Departamento de Nutrição e Dietética, UFF, Niterói, RJ, Brazil
IVProfessor associado, Departamento de Fisiologia, UFF, Niterói, RJ, Brazil
VProfessor associado, Departamento de Estatística, UFF, Niterói, RJ, Brazil
OBJECTIVES: To assess the effect of flaxseed on rat milk creamatocrit and its contribution to offspring weight gain during lactation.
METHODS: The study was conducted with 22 Lister Hooded rats divided into two groups: Control Group (CG, n = 11), which received a casein-based diet with 17% protein, and Flaxseed Group (FG, n = 11), which received a 25% flaxseed diet supplemented with 14% casein, totaling 17% protein. Food consumption was controlled per individual cage and litter size. Maternal milk creamatocrit and offspring weight variation until weaning age were also evaluated.
RESULTS: FG was similar to CG concerning food intake (FG = 76.46±31.87 g; CG = 76.7±33.36 g; p = 0.9613) and equivalent to CG concerning litter size (FG = 4.94±2.34; CG = 5.5±3.19; p = 0.435). The same was found for milk fat content (FG = 18.4±4.76; CG = 15.3±6.03; p = 0.204) and total energy value (FG = 212.92±46.4; CG = 181.1±60; p = 0.1964). FG was similar to CG both in offspring body weight at weaning (FG = 37±6.96 g; CG = 32.6±7.5 g; p = 0.1817) and in weight gain (FG = 31.8±7.0 g; CG = 27.7±7.5 g; p = 0.2104).
CONCLUSION: A total of 25% flaxseed promoted an adequate offspring growth.
Keywords: Flaxseed, casein, rat, creamatocrit, omega-3, body growth.
During pregnancy, the maternal placental and fetal needs for long chain polyunsaturated fatty acids are high. Therefore, a maternal diet with the proper intake of those lipids during gestation and lactation is extremely important. This is especially true for the omega-3 lipids, particularly the docosahexaenoic acid (DHA), once the largest need for this lipid occurs during intrauterine life, mainly during the last trimester of pregnancy and in the pups first months of life, when its largest accumulation in the brain membranes and in the retina occurs.1,2
Some studies also highlight the functional properties of flaxseed (Linum usitatissimum), which is an excellent source of protein, fiber, and alpha-linolenic fatty acid (ALA) (n-3), presenting 32 to 45% lipids in its composition, of which 51 to 55% corresponds to ALA.3,4 This compound has protective effects against non-communicable chronic diseases due to its antioxidant, anti-inflammatory and anti-cancerous actions, among others.5
According to the characteristics described, the objective of this study is to assess the effects of the flaxseed intake during lactation and gestation on the milk creamatocrit of Lister Hood rats and its contribution to offspring body growth during breast-feeding.
The experiment was conducted according to the Brazilian Society of Science in Laboratory Animals (Sociedade Brasileira de Ciência em Animais de Laboratório) determinations and was approved by the Animal Research Ethics Committee of the institution, Universidade Federal Fluminense UFF, under protocol number 00110/09.
The study used 22 Lister Hooded rats, in fertile age, from the Laboratory of Experimental Nutrition, of the Department of Nutrition and Dietetics, School of Nutrition, UFF, Niterói, state of Rio de Janeiro, Brazil.
The rats were divided into two groups: Control Group (CG), comprising 11 animals that had an intake of 17% casein-based protein; and Flaxseed Group (FG), comprising 11 animals that had an intake of 17% protein supplemented with 25% flaxseed, to complete the protein content, and with tert-butylhydroquinone (BHT) antioxidant (0.014 g/kg diet) to prevent the rancidification of the food. This sample size of each group guaranteed a 0.05 significance level and 0.8 power.
All diets were isocaloric and isonitrogenous, balanced and prepared according to the recommendations of the American Institute of Nutrition,6 as shown in Table 1.
Rats received the respective experimental diets since mating and were kept in individual cages, with constant temperature (228±2 ºC) and controlled lighting, light-dark cycle 12/12 h, receiving water ad libitum. During all the period of the test, which lasted 21 days, litter size per group, food intake and offspring body weight were monitored, twice a week, in a digital MF-3 FilizolaTM scale, with a sensitivity of 0.5 g. Pups were separated from their mothers at 21 days old, weaning age, and mothers received Xylazine anesthetic (20 mg/kg) and Oxytocin (Naox) intraperitoneal injections for milking and milk collection.
Creamatocrit values were measured by the technique developed by Lucas et al.7: after the expressed milk (1 ml) homogenization, it was heated in water bath at 40 ºC for 10 minutes, centrifuged at 3.500 rpm for 15 minutes, to separate the cream layer from the serum layer, and then, the fat concentration of the milk and its total energy value were calculated.
The data were presented in the mean and standard deviation forms and were assessed with the S-Plus 8.0. Software. The Student t test was used to analyze food intake and creamatocrit, and for the analysis of litter size per group and offsprings body weight, the Wilcoxon rank-sum test was used, at p < 0.05 level.
During gestation and lactation, Table 2 shows that the FG had the same food intake as the CG group (p = 0.551), as well as similar litter size (p = 0.435). After the analysis of the creamatocrit, it was also observed that there were no differences between the groups (p = 0.204).
In the present study, there was no significant difference between the groups in relation to the rats food intake, and this similarity may be explained by the addition of a small quantity of antioxidant in the flaxseed-based diet, improving palatability and therefore, promoting a better consumption for the animals.
Troina et al.5 also found no difference in food intake between the flaxseed and casein experimental groups during lactation, what is in line with the data in this study.
Flaxseed is a rich source of secoisolariciresinol diglycoside (SDG), a plant lignan and precursor of mammal lignans, which acts positively on the human body, helping to prevent and treat cancer. However, in specific periods such as pregnancy, the sensitivity to hormones is increased, so the administration of lignans should be done with caution, because of its phytoestrogen properties.8 Studies report that these substances may be related to infertility and hyperestrogenism in some species of animals.9
In the present study, both groups were similar in relation to the litter size. Tou et al.10 found the same fact for the same experimental groups, as well as Collins et al.,11 who also found no adverse effects on rats fertility, fetal development and litter size when they fed the rats with flaxseed in high concentrations during pregnancy, lactation and post-lactation.
Fat in maternal milk represents the largest source of energy for the infant, accounting for 40 to 55% of total energy intake, as well as the lipids represent the best source of energy for the animals, since besides providing metabolic energy, they are also needed to maintain the structure and function of the cell membrane and to provide them with the essential fatty acids.12,13
In this study, breast milk creamatocrit of rats fed with flaxseed proved higher to that of the CG both in fat content and in total energy value, though this superiority was not significant; however, it should be highlighted that the importance lies in the quality of breast-milk fat, especially in the delivery of fatty acids, and not in the amount of fat.
Over the last decades, special attention has been given to the composition and the physiological aspects of the lipid fraction of breast milk, especially to the quality of lipids in maternal diet, as they have a direct influence on the expressed milk fatty acids profile, being crucially important for infant nutrition during lactation.14
In the experiment conducted by Almeida et al.,15 it was verified that the diet supplemented with 25% flaxseed presented higher concentrations of ALA (C18:3 n-3) when compared to the casein-based diet. The same authors also found a significant increase of ALA, besides eicosapentaenoic acid (EPA) (C20:5 n-3) and DHA (C22:6 n-3) in the milk of flaxseed-fed rats compared to that of casein-fed rats, a fact which demonstrated the conversion efficiency of ALA to DHA and proved that the maternal dietary lipid quality influences the fatty acids profiles in expressed milk.
This finding may suggest that the breast milk of rats fed with a diet supplemented with 25% of this oilseed might have had an increased intake of n-3 fatty-acids in the present study.
At the same time, Visentainer et al.16 verified that the diet prepared with flaxseed oil presented higher concentrations of ALA, when compared to diets prepared with other oils, concluding that the flaxseed is the richest oilseed in n-3 fatty-acids, a fact that also suggests that, in the given study, the FG diet might have had a higher concentration of ALA.
In another study on the subject,17 it was observed that a diet rich in n-3 fatty-acids of 31 Brazilian nursing mothers contributed to elevate the content of such fatty-acids in milk.
Silva et al.18 also observed that a high consumption of polyunsaturated fatty-acids in eight Brazilian nursing mothers during 10 weeks reflected on a higher content of linolenic acid (n-6) and ALA in milk.
Costa & Sabarense,19 in their review study, found that the milk of Brazilian nursing mothers, compared to that of nursing mothers in other countries, presents a better profile of essential fatty acids and its metabolites (arachidonic acid n-6; e DHA), besides a lower quantity of trans and saturated fatty acids, what led them to conclude that Brazilian maternal nutrition is possibly the main factor contributing to this.
Growth is a dynamic and continuous process that occurs from conception until the end of life and is expressed by the increase in body size.20 A dietary shortage, especially in protein, causes a reduction in food consumption, leading to a decrease in body weight and a delay in weight gain.21 In the present study, it can be observed that pups fed by mothers which received flaxseed diets had greater body weight and weight gain, revealing that this seed is an excellent source of protein and fat, although this superiority is not statistically significant.
According to Almeida et al.,22 in 100 g of flaxseed, there are 396 kcal, 109 from protein and 287 from lipids, and such values were considered excellent for a plant source. In the study performed with 21-day-old pups from the same breed, but from the Wistar lineage, it was observed that the animals from mothers who also received a diet supplemented with 25% flaxseed presented lower body weight than the CG animals (CG = 47.31±4.72 g; FG = 42.69±3.06 g), as well as a lower weight gain (CG = 41.45±4.81; FG = 36.55±3.82).23 These results are distinct from the present experiment; both in body weight (CG = 32.6±7,5 g; FG = 37±6.96 g) and in weight gain (CG = 27.7±7.5; FG = 31.8±7.0).
Soares et al.24 found that by using flaxseed as the only protein source during offspring growth, without adding any animal protein, there was a lower weight gain for the flaxseed-fed group when compared to the casein-fed group; a fact which led them to considerate this oilseed of low protein quality, because it interfered negatively in the animals body growth.
Leite et al.,25 however, considered this oilseed a good source of protein, once it promoted normal growth for the pups, even though they found lower body growth for animals which received flaxseed-based diets compared to those receiving casein-based diets during growth period.
Therefore, it can be concluded that flaxseed proved to be a good source of protein and lipids, promoting adequate growth in animals during lactation. However, more studies should be performed on the omega-3 fatty acids transfers from flaxseed to breast milk.
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Correspondence: Manuscript submitted Jul 21 2011, accepted for
publication Sep 13 2011. Financial support: Fundação de
Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) e Programa de
Apoio a Planos de Reestruturação e Expansão das Universidades
Carine Danielle Ferreira Costa Leite
Rua Tiradentes, 107/506 - Ingá
CEP 24210-510 - Niterói, RJ - Brazil
Tel.: +55 (21) 2629.9860, +55 (21) 8387.0222
No conflicts of interest declared concerning the publication of this article.
Suggested citation: Leite CD, Vicente GC, Suzuki A, Pereira AD, Boaventura GT, dos Santos RM, et al. Effects of flaxseed on rat milk creamatocrit and its contribution to offspring body growth. J Pediatr (Rio J). 2012;88(1):74-8.
Manuscript submitted Jul 21 2011, accepted for publication Sep 13 2011.
Financial support: Fundação de
Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) e Programa de
Apoio a Planos de Reestruturação e Expansão das Universidades