Print version ISSN 0031-1049
Pap. Avulsos Zool. (São Paulo) vol.51 no.7 São Paulo 2011
An unusual, dwarf new species of Neotropical freshwater stingray, Plesiotrygon nana sp. nov., from the upper and mid Amazon basin: the second species of Plesiotrygon (Chondrichthyes: Potamotrygonidae)
Marcelo R. de Carvalho; Maíra P. Ragno
Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, nº 101, CEP 05508-900, São Paulo, SP, Brasil. E-mail: email@example.com. E-mail: firstname.lastname@example.org
A new species of the relatively poorly known Neotropical freshwater stingray genus Plesiotrygon Rosa, Castello & Thorson, 1987 is described from the main channel and smaller tributaries (Ríos Itaya and Pachitea) of the upper Amazon basin in Peru. The first specimen to be collected, however, was from much farther east in Rio Solimões in 1996, just down-river from Rio Purus (specimen unavailable for this study). Plesiotrygon nana sp. nov., is a very distinctive and unusually small species of freshwater stingray (Potamotrygonidae), described here mostly from three specimens representing different size classes and stages of sexual maturity. Plesiotrygon nana sp. nov., is distinguished from its only congener, P. iwamae Rosa, Castello & Thorson, 1987, by numerous unique features, including: dorsal coloration composed of very fine rosettes or a combination of spots and irregular ocelli; very circular disc and snout; very small and less rhomboidal spiracles; short snout and anterior disc region; narrow mouth and nostrils; denticles on dorsal tail small, scattered, not forming row of enlarged spines; adult and preadult specimens with significantly fewer tooth rows; fewer caudal vertebrae; higher total pectoral radials; very small size, probably not surpassing 250 mm disc length or width, males maturing sexually at around 180 mm disc length and 175 mm disc width; distal coloration of tail posterior to caudal stings usually dark purplish-brown; and features of the ventral lateral-line canals (hyomandibular canal very narrow, infraorbital and supraorbital canals not undulated, supraorbital and infraorbital loops small and narrow, supraorbital loop very short, not extending posteriorly to level of mouth, jugular and posterior infraorbital canals short, not extending caudally to first gill slits, subpleural loop very narrow posteriorly; absence of anterior and posterior subpleural tubules). To provide a foundation for the description of P. nana sp. nov., morphological variation in P. iwamae was examined based on all type specimens as well as newly collected and previously unreported material. Two specimens topotypic with the male paratype of P. nana sp. nov., referred to here as Plesiotrygon cf. iwamae, are also reported. Relationships of the new species to P. iwamae are discussed; further characters indicative of Plesiotrygon monophyly are proposed, but the genus may still not be valid. Plesiotrygon nana sp. nov., is commercialized with some regularity in the international aquarium trade from Iquitos (Peru), an alarming circumstance because nothing is known of its biology or conservation requirements.
Key-Words: Plesiotrygon iwamae; Taxonomy; Morphology; Myliobatiformes; Rio Solimões; Río Ucayali; South America.
Uma nova espécie do relativamente desconhecido gênero de raia de água doce Neotropical Plesiotrygon Rosa, Castello & Thorson, 1987 é descrita do canal principal e de tributários menores (Ríos Itaya e Pachitea) do alto da bacia Amazônica do Peru. O primeiro exemplar coletado, porém, foi capturado em 1996 muito mais ao leste no Rio Solimões, pouco abaixo da sua confluência com o Rio Purus (exemplar não disponível para estudo). Plesiotrygon nana sp. nov., é uma espécie pequena, muito distinta e incomum de raia de água doce (Potamotrygonidae), descrita aqui principalmente a partir de três indivíduos representando diferentes classes de tamanho e estágios de maturação sexual. Plesiotrygon nana sp. nov., distingue-se da única espécie previamente conhecida, P. iwamae Rosa, Castello & Thorson, 1987, por inúmeras características únicas, incluindo: coloração dorsal composta por rosetas muito finas ou uma combinação de pontilhados e ocelos irregulares; disco e focinho muito circular; espiráculos rombóides e bem pequenos; focinho curto; boca e narinas estreitas; dentículos no dorso da cauda pequenos, dispersos, sem formação de fileira de espinhos desenvolvidos; espécimes adulto e pré-adulto com um número significativamente menor de fileiras de dentes; menos vértebras caudais; maior número total de raios peitorais; tamanho muito pequeno, provavelmente não ultrapassando 250 mm de comprimento ou largura de disco; maturidade sexual dos machos perto de 180 mm de comprimento de disco e 175 mm de largura de disco; coloração distal da cauda posterior ao espinho caudal normalmente marrom escuro arroxeado; e características dos seus canais ventrais da linha lateral (canal hiomandibular delgado, canais infraoribital e supraorbital não ondulados, voltas supraorbital e infraorbital pequenas e estreitas, volta supraorbital não se estendendo posteriormente ao nível da boca, canais jugular e infraorbital posterior pequenos, não se estendendo caudalmente ao primeiro par de fendas branquiais, volta subpleural muito delgada posteriormente). Para fundamentar a descrição de P. nana sp. nov., variações morfológicas em P. iwamae foram examinadas baseadas em todos os exemplares da série tipo e em outros recém-coletados mas até então não reportados. Dois espécimes topotípicos com o parátipo macho de P. nana sp. nov., aqui referidos como Plesiotrygon cf. iwamae, também são descritos. Relações evolutivas da espécie nova com P. iwamae são discutidas; caracteres indicativos da monofilia do gênero são propostos, mas este pode ainda não ser válido. Plesiotrygon nana sp. nov., é comercializada com alguma freqüência no mercado internacional de peixes ornamentais de Iquitos (Peru), uma circunstância alarmante já que nada é conhecido sobre sua biologia ou possíveis diretrizes para conservação.
Palavras-chave: Plesiotrygon iwamae; Taxonomia; Morfologia; Myliobatiformes; Rio Solimões; Rio Ucayali; América do Sul.
The Neotropical freshwater stingray Plesiotrygon iwamae Rosa, Castello & Thorson, 1987 first appeared in the literature, misidentified as Elipesurus strogylopterus (not of Schomburgk, 1843), in P. de Miranda Ribeiro's (1959) catalogue of the fish collection of the Museu Nacional (Rio de Janeiro). Miranda Ribeiro's specimen, of unknown origin, is a partially mutilated juvenile male lacking the tail that, unfortunately, was a poor representative of this unusual stingray. It is no wonder that almost three decades went by before the genus and species were formally described (Rosa et al., 1987); in between these accounts, there was a single literature record, but again misidentified (as Potamotrygon scobina; Taniuchi, 1982). Rosa et al. (1987) provided a thorough description of P. iwamae based on six specimens collected primarily between 1969 and 1981 and deposited in museums in Brazil and North America; the first specimen of P. iwamae to become available for study was the paratype in Hamburg collected in 1909. Plesiotrygon iwamae continued to be rare both in collections and the specialized literature even after being described. But more recent collecting efforts have revealed that P. iwamae is a widespread Amazonian species, distributed from Peru and Ecuador to the much lower waters of Rio Pará near the mouth of Rio Amazonas, occurring mostly in its main channel, but capable of entering lower parts of its major tributaries (such as in Rio Purus).
With such a broad distribution within the Amazon basin, and based on a much larger series of specimens than was previously available, we set out to discover if P. iwamae concealed a greater (and undescribed) cryptic diversity. Our studies led us to examine the stingray holdings of the Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, in Lima (Peru). Although no specimens of P. iwamae were found, a much smaller, darker individual very distinctive from "typical" representatives of P. iwamae, was located. This unique specimen appeared to be conspecific with a smaller individual captured during the 1996 Calhamazon Project in Rio Solimões just below its confluence with Rio Purus (for a summary, see Fernandes et al., 2004), a photograph of which was examined by the senior author in that same year (this specimen, however, was unavailable for this study). The Lima specimen also resembled a small female individual in our possession, collected in 2009 by a colleague from Río Itaya, a tributary of Río Nanay in the vicinity of Iquitos (Peru). These specimens, along with another one collected in the Río Amazonas of Peru in late 2010, agree with a form called the "black-tailed" or "dwarf" antenna ray in the aquarium literature (e.g. Ross & Schäfer, 2000), and are described below as only the second species of Plesiotrygon. Our work is based on a thorough examination of the variation present in P. iwamae across its range, which unveiled noteworthy morphological distinctions between both species, such as in size, coloration, disc shape, proportions of nasoral region, spiracles and snout, ventral lateral line canals, and dermal covering; results of our study on P. iwamae are not presented in full here, but are forthcoming.
Material and Methods
Counts of vertebrae and fin rays taken from specimens are based on Compagno & Roberts (1982) and Carvalho & Lovejoy (2011). Tooth rows were counted following Stehmann et al. (1978) on preserved specimens and from radiographs (and include all tooth rows, not just those exposed). Terminology for skeletal morphology follows Nishida (1990) and Carvalho et al. (2004), and for lateral-line canals Garman (1888), Ewart & Mitchell (1892), and Chu & Wen (1979). Anatomical abbreviations are given in figure legends. Skeletal structures were studied from material of all species of Potamotrygonids, through radiographed, dissected, and cleared and stained specimens (prepared following Dingerkus & Uhler, 1977).
Measurements follow classic protocols for batoids established in Bigelow & Schroeder (1953) and Hubbs & Ishiyama (1968), and further modified for Potamotrygonids by Rosa (1985) (see also Carvalho & Lovejoy, 2011; Silva & Carvalho, 2011). Measurements, taken point to point, are presented in Tables 1, 3 and 5 as both raw data in mm and transformed into % DW; measurements are as follows: total length, distance from tip of snout to tip of tail; disc length, greatest distance from tip of snout to posterior margin of disc; disc width, greatest distance between lateral margins of disc; interorbital distance, distance between upper margins of eyes; interspiracular distance, distance between posterior margins of spiracles; eye length, greatest horizontal diameter of exposed portion of eyeball; spiracle length, oblique distance between anterior and posterior spiracular margins; preorbital length, distance from tip of snout to anterior margin of eyes; prenasal length, distance from tip of snout to anterior margin of nostrils; preoral length, distance from tip of snout to median portion of mouth slit; internarial distance, distance between anterior margins of nostrils; mouth width, greatest distance between lateral edges of mouth; distance between 1st gill slits, distance between inner margins of first pair of gill slits; distance between 5th gill slits, distance between inner margins of fifth pair of gill slits; branchial basket length, distance between outer margin of first and inner margin of fifth gill slits; pelvic fin anterior margin length, length of anterior margin of pelvic fin; pelvic fin width, greatest width between posterior margins of pelvic fins; clasper external length, distance from posterior margin of pelvic fin to tip of clasper; clasper internal length, distance from posterior margin of cloaca to tip of clasper; distance between cloaca and tail tip, distance from posterior margin of cloaca to tip of tail; tail width, greatest width of base of tail; snout to cloaca distance, distance from tip of snout to proximal margin of cloaca; pectoral to posterior pelvic length, distance from pectoral axil, at the joint of posterior margin of disc and tail, to posterior margin of pelvic fin; distance from cloaca to sting origin, distance from posterior margin of cloaca to base of first caudal sting; sting length, length of exposed portion of caudal sting (dorsalmost sting when more than one is present); sting width, width taken at the origin of caudal sting (dorsalmost sting when more than one is present). Abbreviations in text: DL, disc length; DW, disc width; TL, total length. Meristic features are presented in Table 2.
Material of Plesiotrygon examined is deposited in the Field Museum of Natural History, Chicago (FMNH); Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Lima (formerly MHNJP) (MUSM); Museu Nacional, Rio de Janeiro (MNRJ); Museu de Zoologia da Universidade de São Paulo, São Paulo (MZUSP); National Museum of Natural History (NMNH), Smithsonian Institution, Washington DC; and Zoological Museum of Hamburg, Hamburg (ZMH). Collected specimens were fixed in 10% formalin and subsequently transferred to 70% ethanol. Comparative material of Paratrygon aiereba deposited in the MZUSP (which does not amount to all Paratrygon material examined) is listed in Carvalho & Lovejoy (2011). Comparative material of Potamotrygon spp. examined is mostly deposited in MZUSP, representing all valid species except P. magdalenae (Valenciennes, 1865). Historical material of Potamotrygonidae, including type specimens in European and South American museums, has also been examined. Other examined material is listed in Carvalho et al. (2004).
Family Potamotrygonidae Garman, 1913
Genus Plesiotrygon Rosa, Castello & Thorson, 1987
A genus of Potamotrygonidae distinguished from the other genera of the family (Paratrygon Duméril, 1865, Potamotrygon Garman, 1877, and Heliotrygon Carvalho & Lovejoy, 2011) by the following characters: pelvic fins projecting posteriorly well beyond posterior disc margins (about one-half of pelvic fin length visible dorsally); distal caudal filament extremely long, much longer than disc (usually more than twice disc length), even in large adult specimens (distal caudal filament rarely broken off even in large adult specimens); eyes very small, not protruding above disc; ventral tail-fold well developed, almost as tall as tail, but lacking dorsal fold; caudal stings well developed and positioned relatively far posterior on dorsal tail; single, very broad, robust and relatively short angular cartilage; relatively few pectoral fin radials (total radials ranging from 75-91).
The characters listed above are present in both Plesiotrygon iwamae and the new species, described below. Of these characters, the posterior placement of the pelvic fins, the extremely long caudal filament, posterior position of caudal stings, and the well developed ventral tail fold are unique among Potamotrygonids. But angular cartilage morphology and a relatively lower number of pectoral fin rays in Plesiotrygon require further comment.
Paratrygon and Heliotrygon lack angular cartilages, which are present in all species of Potamotrygon (Garman, 1913; Rosa, 1985; Carvalho et al., 2004; Carvalho & Lovejoy, 2011; Carvalho et al., in press). Most species of Potamotrygon, however, have two angular elements associated to the hyomandibular-Meckelian ligament (in between the hyomandibula and Meckel's cartilage). These angulars are usually of similar dimensions, and the anterior angular frequently is strongly concave, features that are clearly distinct from the single, stout angular of Plesiotrygon. Also distinct are the angulars observed in specimens of P. signata Garman, 1913, which have a very wide anterior angular element and reduced, concealed posterior component. A single angular cartilage is present in Potamotrygon schroederi Fernandez-Yépez, 1958, P. orbignyi (Castelnau, 1855), P. humerosa Garman, 1913, P. histrix (Müller & Henle, 1834), P. marinae Deynat, 2006, and P. tigrina Carvalho, Sabaj & Lovejoy, 2011, but it is not as broad and stout as in species of Plesiotrygon (variations in the angular and their systematic significance are presently being studied).
The relatively low number of pectoral fin radials in species of Plesiotrygon, varying from 75-91 total radials, is much lower than in Paratrygon and Heliotrygon, which have counts upwards of 100 total pectoral radials (Rosa, 1985; Carvalho & Lovejoy, 2011). Some species of Potamotrygon have pectoral radial numbers more comparable to Plesiotrygon, such as P. scobina Garman, 1913, and P. magdalenae. But P. brachyura (Günther, 1880) has much higher counts (typically at least 106, according to radiographed MZUSP specimens), and P. falkneri Castex & Maciel, 1963, P. orbignyi, P. humerosa Garman, 1913, P. marinae, and P. motoro (Müller & Henle, 1841) have slightly higher counts ranging from about 93-105 (Loboda, 2010; Silva, 2010; Silva & Carvalho, 2011). Even though Plesiotrygon and many species of Potamotrygon are similar in pectoral radial numbers, whether this is significant in terms of uniting both genera is still undetermined. Rosa (1985: 410) united Potamotrygon and Paratrygon as a monophyletic group on the basis of high modal number of pectoral fin radials, but this feature is somewhat continuous with Plesiotrygon, and species of Potamotrygon vary slightly in this regard.
Plesiotrygon iwamae Rosa, Castello & Thorson, 1987 by original designation.
MUSM 20328, adult male (1024 mm TL, 243 mm DL, 247 mm DW), Río Pachitea, tributary of Río Ucayali, up-river from town of Puerto Inca, Puerto Inca Province, Huánuco Department, Peru, 09º25'S, 74º55'E, 15 August 2002, coll. Edgardo Castro (Figures 1, 2, 8, 9, 11, 13-15).
MUSM 40243, preadult male (671 mm TL, 174 mm DL, 170 mm DW), Río Amazonas, Aucayo Caserio, near Tamshiyacu, Sargento Lores District, Maynas Province, Loreto Department, Peru, 03º59'13.21"S, 73º10'02.80"W, altitude 89 m, 15 November 2010, coll. Homero Sanchez; MZUSP 108777, juvenile female (463 mm TL, 81 mm DL, 72 mm DW), Río Itaya, tributary of Río Nanay (itself an affluent of Río Amazonas), near Iquitos, Departamento Loreto, Peru, 18 October 2009, coll. F. Marques (PU 09-45) (Figures 3-7, 11, 12, 27).
Non type specimen
MZUSP 57642, juvenile or preadult female, 912 mm TL, 124 mm DL, 118 mm DW, from Rio Solimões, just down-river from the confluence with Rio Purus (Brazil), 03º36'22.9"S, 61º20'14.0"W, to 03º36'26.0"S, 61º19'52.3"W, 1996 Calhamazon Project, 28 Jul 1996, coll. A. Zanata et al., collected 50 m from shore over predominantly clay bottom (AMZ-96-081).
A species of Plesiotrygon diagnosed by the following unique characters: (1) dorsal color pattern composed of a dark gray to dark brown background color, with tan to yellow highly curved, slender and convoluted stripes or small spots forming rosette-like pattern over dorsal disc, or with creamy white to yellowish irregularly shaped, scattered spots and ocelli, smaller than interorbital distance (pale gray or brown dorsal background color in P. iwamae, composed of faint, incomplete markings outlined by small spots, or small irregular blotches and creamy, faint spots); (2) disc circular in preadults and adults, about as wide as long (markedly oval in P. iwamae in all sizes); (3) broadly rounded anterior disc (anterior disc sharply oval in P. iwamae); (4) spiracles only faintly rhomboidal, very small, ranging from 2.8 to 3.5% DW in adult and preadult specimens (spiracle strongly rhomboidal, with mean spiracle length 6.8% DW in P. iwamae); (5) snout very short, mean preorbital length 21.2% DW, mean prenasal length 15.2% DW, and mean preoral length 18.7% DW (snout proportionally much more elongate in P. iwamae, with mean preoral length 27.4% DW, mean prenasal length 19.5% DW, and mean preoral length 25% DW); (6) mouth and nostrils very slender especially in adult and preadult specimens, with mean mouth and internarial width 6.3% DW (mouth and internarial distance much greater in P. iwamae, with mean mouth width 10.9% DW, and mean internarial distance 8.7% DW); (7) denticles on dorsal tail relatively small, scattered, not forming row of greatly enlarged spines (P. iwamae usually with a single irregular row of enlarged spines on dorsal tail region); (8) adult and preadult specimens with few (20-21/19) tooth rows (adult specimens of P. iwamae have numerous tooth rows, ranging from about 40-60/42-64); (9) caudal vertebrae ranging from 86-88, with a modal count of 86 (93-98, with a modal count of 94 caudal vertebrae in P. iwamae); (10) total pectoral radials in adult and preadult specimens 90-91 (in P. iwamae, total pectoral radials varied from 77-84); (11) overall size very small (probably not surpassing 250 mm DL or DW), males sexually maturing probably between 180 and 220 mm DL, and 175 and 225 mm DW (P. iwamae attains great sizes, upwards of 650 mm DL or DW, reaching sexual maturity only at about 420 mm DL or DW); (12) distal coloration of tail, as of caudal stings, usually a dark purplish brown, remaining this color to extremity of whip (in P. iwamae, tail as of caudal stings creamy white ventrally and light gray dorsally, with creamy white distal whip); (13) ventral lateral-line canals with the following unique characters: hyomandibular canal very narrow, with external and internal components close together; infraorbital and supraorbital canals not undulated; supraorbital and infraorbital loops small and narrow, without wavy contours; supraorbital loop of anterior infraorbital canal very short, not extending posteriorly to level of mouth; jugular and posterior infraorbital canals short, not extending posteriorly to close to gill slits; subpleural loop very narrow posteriorly; anterior and posterior subpleural tubules of hyomandibular canal absent (for comparison with ventral lateral-line canals in P. iwamae and other anatomical differences between both species, as well as further details concerning features listed here, see Discussion below).
Disc very circular, about as long as wide, and widest at more or less midlength, near level of scapulocoracoid (for description below, refer to Figures 1-10). Disc length 98.4% DW in holotype, ranging between 98.4 and 112.5% DW (mean 104.4% DW) (Table 1). Disc very low and flat, tallest at head region. Snout with broadly rounded anterior margin, and with a minute but clearly visible, rostral knob-like projection protruding from anterior disc. Rostral knob somewhat fleshy, well developed in all specimens. Snout length considerably small. Preorbital snout length less than one-fourth disc width, slightly less than twice interorbital distance; preorbital snout length from 20.0 to 22.7% DW (mean 21.2% DW). Prenasal (12.1 to 18.1% DW, mean 15.2% DW) and preoral (16.2 to 22.2% DW, mean 18.7% DW) snout lengths also relatively short, shorter than preorbital snout length. Eyes very small, slightly rounded, protruding very little from top of head and disc in live specimens (see Figure 27); eyes smaller than spiracles in diameter. Spiracles closely adjacent to eyes, oval-rounded, not very rhomboidal, and relatively small. Spiracles without elevated spiracular rims or central knob posteriorly. Interspiracular distance slightly greater than interorbital distance.
Mouth very small and narrow, with opening somewhat straight across. Mouth width less about one-third distance between first gill slits, and more or less equal to internarial distance. Two small labial folds present at outer jaw corners posterior to tubular narial fold, extending anterolaterally away from jaw corners. Nostrils anteroposteriorly elongated, slit-like, close in length to internarial distance. Nasal curtain very narrow, straight and not widening posteriorly, with highly fringed and medially notched posterior margin (Figure 8). Rounded, tubular narial fold present lateral to posterior corners of nasal curtain. Teeth set in quincunx, not visible externally with mouth closed in holotype. Tooth rows 20/19 in holotype, 21/19 in male paratype, and 13/16 in much smaller female paratype; teeth small, rhomboidal, with small cusps in holotype (an adult male). Five buccal papillae present inside mouth. Branchial basket about twice as wide as long, its length about one-tenth DW. Distance between first gill slits slightly greater than distance between fifth gill slits. Gill openings slightly obliquely positioned, very small; fifth gill slit smallest.
Pelvic fins with broadly rounded outer margins, much wider than long (48.6 to 57.6% DW, mean 54.0% DW), and with undulating posterior margins. Pelvic fins protruding significantly from posterior disc region, and triangular in dorsoventral view, broadest at more or less posterior apices. Anterior margins of pelvic fins with marked angle at more or less midlength of anterior surface, more prominent in larger specimens; outer portions of pelvic fins fleshy. Clasper relatively slender, projecting well beyond posterior margin of pelvic fin (Figure 9). Clasper dorsoventrally flattened, with rounded posterior tip. Clasper groove deflects inward toward midline at clasper glans region; dorsal pseudosiphon relatively small, positioned at a slight angle. Hypopyle at beginning of clasper glans, extending posteriorly in a very straight line. Ventral pseudosiphon situated on external margin of clasper tip, about as elongated as hypopyle.
Tail at base about as wide as interorbital distance (tail width 11.1 to 15.3% DW, mean 12.9% DW). Tail strongly tapering from base, terminating far posteriorly as an elongated, filiform whip. In holotype (adult male), disc length and width only about one-fourth of total length; in female paratype, total length almost six times as long as disc length or width; in male paratype distal tail slightly shorter). Tail whip decreasing in diameter posteriorly, terminating as a very small point. Tail dorsoventrally flattened in cross-section throughout, with ventral, medially positioned groove present from tail base, extending very posteriorly beyond caudal stings. Relatively broad ventral tail fold originating within groove at more or less level of caudal sting origin, tallest at more or less midlength of caudal stings, and extending posteriorly for more than twice length of caudal stings. Tail without lateral or dorsal tail folds. Cloaca to distal tail length great (311.2 to 540.3% DW, mean 402.8% DW). Caudal stings positioned far posteriorly on dorsal tail (distance from tail base to their origin greater than one-half of disc width). Caudal stings clearly greater than interorbital distance or tail width at base. Caudal stings very slender, their width in male paratype about one-twentieth their length, but in female paratype caudal sting width about one-tenth of length. Caudal stings with acute distal apex and with posteriorly directed and sharp lateral serrations.
Dorsal color of disc somewhat variable in material studied (Figures 1-7, 10). In live specimens, colors more intense but not significantly different. Dorsal disc with light to dark brown or blackish-brown background color, slightly lighter close to disc margin in preserved material. Background color forming a dark reticulate pattern surrounding tan to yellowish irregular patterns of rosettes, spots and/or incomplete ocelli of alternating sizes. In holotype (largest male specimen), rosettes very conspicuous, formed from very slender and convoluted individual lines and small spots of lighter color. Largest rosettes about equal to interorbital distance in diameter, smaller rosettes about half this size. Larger rosettes present on midlateral disc, reducing in size toward outer disc. Outer disc with interrupted yellow lines, and outermost disc region with numerous very small tan to yellowish spots. Rosettes closely adjacent, and may mesh together forming elaborate patterns over mid and outer disc regions. Rosettes over central disc obscured by intense covering of dermal denticles. Dorsal aspect of pelvic fins with more individual spots, these relatively more isolated, occasionally forming incomplete ocelli. Claspers with creamy white background and irregular, diffuse brown and grayish blotches dorsally, more concentrated on region of hypopyle, clasper groove and dorsal clasper glans; claspers creamy white ventrally. Color of base of tail also obscured by denticles, but with a speckled darker and lighter color, with fewer lighter spots than outer disc. Lateral aspect of tail, anterior to caudal stings, with alternating creamy white and grayish or brown stripes; grayish stripes with diffuse whitish areas within; white stripes with slightly darker areas interspersed. Dorsal aspect of tail also with alternating pattern but much more concealed by denticles, not as sharp. Ventral surface of holotype a uniform creamy white, with slight patches of dusky gray at posterior disc and posterior pelvic fin margins. Ventral tail creamy white until about midlength of ventral tail fold, with darker bands alternating with lighter bands present until about just greater than one-half of tail length. Posterior more or less two-thirds of tail uniformly dark purplish brown; distal tail extremity evenly dark on all sides.
Preadult male paratype with slightly distinct color pattern, with more background color present, and with spots and ocellated markings relatively smaller than in holotype. Spots and ocelli more scattered, much smaller than interorbital distance, and irregularly shaped. Clearly defined rosettes, as in holotype, absent. Spots and dorsal markings tan to yellow, reducing in diameter closer to disc outer margin. Laterally elongated and irregularly shaped spots present on disc. Pelvic fins with similar dorsal pattern as disc, but with slightly more regularly spaced creamy white spots. Claspers also with dorsal pigmentation most concentrated on dorsal clasper glans region, similar to holotype. Base of tail more marked with creamy white bands on lateral aspect, and with more whitish markings compared to holotype. Alternating bands of darker gray and creamy white present distally on tail posterior to caudal stings. Distal filiform tail creamy white ventrally, not dark as in holotype and female paratype. On ventral disc and base of tail, male paratype creamy white throughout.
Female paratype with color pattern more or less similar to male paratype, composed of numerous creamy white to yellowish irregularly shaped spots and ocelli (Figures 5-7). Spots very reduced in size, especially at outer disc. Spots and irregular ocelli greater at middisc region. Incomplete ocelli present mostly over central disc area. Laterally elongated and irregularly shaped spots present on disc. On anterior disc region, fine vermiculate pattern present. Base of tail with darker midline region and dorsolaterally positioned lighter ocelli. Lateral tail with alternating bands of lighter and darker from base of tail, where bands are less defined, to about one-half of tail length; tail uniformly very dark purplish-brown at posterior half to tail extremity. Solimões specimen with similar color pattern, but with more reticulate background, and larger, more rectangular lighter markings on central dorsal disc region; spots diminish in size closer to outer disc margin (Figure 10). Caudal whip also dark purplish-brown in this specimen.
Dermal covering in holotype and preadult male paratype with low and relatively wide, intensely packed dermal denticles on anterior disc and snout, middisc and tail-base regions, with slightly larger, more acute spines on posterolateral disc, and sharp, taller spines on dorsal and lateral aspects of tail from tail base posteriorly to caudal stings (Figures 11, 13, 14C). Larger dorsal disc denticles with stellate bases, usually with five to ten basal ridges radiating from denticle base. Denticles thoroughly covering disc, even on outer disc margins. Denticles larger, with wider bases, and more closely packed on middisc especially over disc midline. Outer disc with slightly enlarged spines, more developed on anterior and posterior outer disc regions; these larger, outer disc denticles evenly spaced apart, relatively straight, not organized in rows, and surrounded by smaller denticles. These smaller denticles are also very erect, without radiating basal ridges. Base of tail region with numerous enlarged spines not forming regular, discrete rows. Enlarged spines much smaller than in P. iwamae, Paratrygon and species of Potamotrygon. Spines sharp, with enlarged, wide bases and short, radiating basal ridges; central crown well developed with acute, posteriorly directed, tall central spine. Spines greatest on anterior dorsal tail base region. Smaller spines present in between larger spines, evenly spaced apart, on base of tail, dorsal tail extending posteriorly to caudal stings, and lateral tail regions. Denticles on lateral tail region also well developed, extending posteriorly well beyond level of caudal stings. Distal tail whip with very minute denticles ("prickles') until more or less midtail area in both male specimens. Dorsal aspect of pelvic fins also with evenly scattered denticles, but smaller and less packed than on disc. Small female paratype with dorsal disc denticles less developed, not as sharp, but denticles numerous, and without developed dorsolateral disc spines or enlarged spines on dorsal and lateral tail regions. Solimões specimen with slightly more denticles and with small spines on dorsal disc and tail base (from photograph).
Ventral lateral-line canals
The principal canals of the ventral lateral-line system resemble those present in Plesiotrygon iwamae (Figure 12) and in species of Potamotrygon, but are very distinct from Paratrygon or Heliotrygon (e.g. Carvalho & Lovejoy, 2011). In general, ventral lateral-line system narrow, occupying small portion of ventral disc between outer disc and branchial slits, but anterolateral disc and snout canals proportionally less narrow. Prenasal canal extends vertically from the nasal curtain to the anterior snout tip, running more or less parallel to its homologous component on the other side. Prenasal is crossed anteriorly by infraorbital canal and is continuous posteriorly with supraorbital canal, where it forms a small, markedly triangular figure on nasal curtain. Infraorbital and supraorbital canals relatively straight, not undulated. Infraorbital canal with very straight, vertical external margin from supraorbital loop to infraorbital loop; supraorbital and infraorbital loops small, relatively narrow and uniform in width, without wavy contours. Supraorbital loop of anterior infraorbital canal very short, not extending posteriorly to level of mouth. Jugular canal somewhat narrow and also not undulated. Jugular and infraorbital canals not very elongate anteroposteriorly, not extending posteriorly to close to first pair of gill slits. Mandibular canal not observed. Hyomandibular canals forming very narrow loop posteriorly. External hyomandibular canal very straight, not curved or inflected, running very close to external infraorbital canal anteriorly and branchial slits at midlength. Internal hyomandibular canal slightly curved medially away from gill slits. Hyomandibular canal widest at more or less its posterior one-fifth where it bulges laterally, anterior to subpleural loop. Subpleural loop very narrow, somewhat acute posteriorly. Anterior and posterior subpleural tubules of hyomandibular canal absent.
Neurocranium elongate, longer than twice greatest width, and widest at postorbital processes and nasal capsules. Neurocanium very slender at orbital region and central cranial floor, strongly tapering posteriorly from nasal capsules to postorbital processes. Nasal capsules relatively large, oval, broadly rounded anteriorly, and slightly inclined toward midline; internasal septum very slender (Figures 13-15). Orbital region very concave. Preorbital processes broadly triangular and posterolaterally oriented. Postorbital processes very elongate, anterolaterally directed, reaching level of angular cartilages anteriorly. Precerebral and frontoparietal fontenellae long, but not as long as usually present in species of Potamotrygon, and about two-thirds of neurocranial length. Supraorbital process relatively wide and broadly triangular, situated just anterior to postorbital process. Neurocranium widens significantly at its posterior third where it articulates with hyomandibulae. Neurocranium very elongate posterior to postorbital processes; its length posterior to postorbital processe about 40% of neurocranial total length. Antorbital cartilage slightly laterally compressed, triangular, very slender and elongate, widest anteriorly, and extending posteriorly to level of palatoquadrates, anterior to angular cartilage.
Meckel's cartilage very stout, with strong anteromedial deflection toward midline. Dorsally projecting lateral process of Meckel's cartilage low and broadly triangular, not slender and elongate. Palatoquadrates very slender, shorter than Meckel's cartilage, and also somewhat inclined toward midline (Figures 14A, B). Hyomandibulae relatively short, slender and more or less straight, widest at midlength; hyomandibula only slightly curved anteriorly toward midline, and faintly concave distally to accommodate hyomandibular-Meckelian tendon. Angular cartilage well developed, very stout, at least as stout as hyomandibula, and relatively short, about one-third length of hyomandibula (Figures 14B, C, 15B). Angular cartilage more or less straight, without concave anterior or posterior margins, slightly thicker closer to Meckel'cartilage. Hypobranchials slender, inclined toward midline, reaching anteriorly to level of postorbital processes of neurocranium. Basihyal element(s) not calcified, not apparent in radiographs. Basibranchial anteriorly triangular, extending forward to level of hyomandibular facet of neurocranium. Pseudohyoid arch more slender than subsequent branchial arches; all branchial arches short, not elongated laterally. Gill rays extending to propterygium.
Cervicothoracic synarcual elongate, its greatest width just under greatest width of neurocranium. Thoracolumbar synarcual very slender, not strongly calcified. Individual vertebral centra occurring posterior to level of caudal sting origin, last discernible centrum at more or less caudal sting extremity. Distal to caudal stings, an uncalcified notochordal extension (cartilaginous rod) present, continuing caudally toward whip extremity. Transition from mono- to diplospondyly occurs at fourth to fifth centra posterior to pelvic girdle.
Propterygium widest posteriorly, more stout than meso- and metapterygium (Figures 13, 14A, 15A). Propterygium anteriorly also relatively wide, much more so than in species of Potamotrygon, extending anteriorly to level of posterior nasal capsules; anterior segment of propterygium smaller than width of nasal aperture, extending forward to almost nasal capsule anterior margin. Metapterygium more slender and slightly more broadly arched than propterygium, with two smaller, more slender posterior segments. Mesopterygium elongate and more slender anteriorly, slightly convex externally, and highly concave internally where it articulates with lateral aspect of scapulocoracoid. Articular surface with scapulocoracoid extensive, more so than in species of Potamotrygon. Mesopterygium extends posteriorly only slightly. Pectoral radial elements sometimes fused at base between mesopterygium and anterior metapterygium. Pectoral radials slender close to pectoral basals, slightly wider and shorter at middisc, and slender again distally; some 16 total lateral pectoral radial segments present (from pectoral basals to outer disc); pectoral basals bifurcating at distal segments 9 and 10. Scapulocoracoid, in ventral view, very elongate anteroposteriorly from where it articulates anteriorly with propterygium to articular area with metapterygium, proportionally much longer than in any Potamotrygonid. Coracoid bar with straight posterior margin but highly concave and relatively narrow anterior border. Articular surface for fifth ceratobranchial on anterior surface of scapulocaoracoid markedly protruding.
Pelvic girdle with concave anterior margins lateral to prepelvic process, more concave than in P. iwamae. Lateral prepelvic processes rather low, not very acute. Iliac processes extending caudally beyond triangular ischial processes; both structures triangular and relatively slender. Puboischiadic bar anteroposteriorly elongate at sides, more so than in P. iwamae and species of Potamotrygon. Posterior margin of puboischiadic bar very concave, highly oval, extending posteriorly to a significant degree (Figure 13). Three to four obturator foramina present. Basipterygium relatively wide, tapering posteriorly, about equal in length to one-half of puboischiadic bar width. First enlarged pelvic radial element articulating with lateral projection of iliac region, and about twice thickness of following radial segments. Pelvic radials subdivided laterally into three or four segments; segment contacting basipterygium much longer than others. Pelvic fin widest at sixth pelvic radial. Posteriormost radials articulating with basipterygium splayed. Clasper not dissected for skeleton, but in radiographs two basal segments discernible.
The variation in dorsal color pattern observed among the three type-specimens of Plesiotrygon nana, although seemingly significant, is not enough to consider them separate species. Nor is the distinction in color pattern between the Rio Solimões specimen (Figure 10) and those from Peru very great. All specimens represent different size-classes and stages of sexual maturity (and all come from different localities, although the paratypes were collected not too far from each other). The paratypes are more similar in color, with more isolated irregular spots and incomplete ocelli present on dorsal disc. In the preadult male, the light yellow dorsal markings are relatively more spaced-apart, which, in relation to the smaller female, represents a pattern that can be achieved with growth. The holotype, though, is somewhat distinct in dorsal color. The irregular rosettes formed by very slender lines and small spots on disc are not present in the other specimens. The preadult male (174 mm DL, 170 mm DW) is significantly smaller than the holotype (243 mm DL, 247 mm DW), but it is difficult to envision its color changing to resemble the holotype if it were to have kept growing, although this cannot be discarded based on our small sample size. Of the four specimens depicted in one aquarium source (Ross & Schäfer, 2000: 140, 144), one has similarities in color with the male paratype (p. 144, figure on lower right), and another resembles the holotype to some degree (p. 140, figure on top). Clearly, P. nana should be expected to be somewhat variable in dorsal color.
The creamy white distal tail extremity in the preadult male paratype (MUSM 40243; Figure 3) is, however, more difficult to account for. The caudal filiform whip in this specimen resembles that of the holotype from tail base to more or less level of caudal stings. The mid region of the whip in the holotype, however, has a predominantly dark grayish color, even where it is alternately banded with lighter stripes anterior to its midlength. But in MUSM 40243 the mid caudal whip region beyond the caudal stings is predominantly light colored ventrally, and remains so to caudal extremity. In contrast, in the holotype, the smaller female paratype and the Solimões specimen, the caudal whip becomes a uniform dark purplish-brown anterior to its midlength and remains this color posteriorly to caudal extremity. All aquarium specimens depicted in the literature (e.g. Ross & Schäfer, 2000) also have a very dark caudal whip for about two-thirds of tail length (hence one of their popular names, "black-tailed" antenna ray). Plesiotrygon iwamae, on the other hand, has a much lighter distal tail from level of caudal stings posteriorly (for about two-thirds tail length), and even when darker, the tail of P. iwamae is much lighter than the tail of P. nana. At present we can only conclude that this feature may be variable, even if minimally so (note that the holotype of P. iwamae has a grayish white distal tail whip, slightly darker than other specimens from Rio Solimões and from Peru). The preadult male paratype (MUSM 40243) clearly shares many diagnostic features with the holotype and female paratype of P. nana, and cannot be identified with P. iwamae.
There is also variation in disc shape among the four specimens of Plesiotrygon nana. The small female paratype has a markedly oval disc, but the Solimões specimen has a rounder disc, whereas both larger males have clearly rounded discs (especially the holotype). These variations are ontogenetic, and not sexually dimorphic, as larger females depicted in the aquarium literature also have more circular discs compared to the small female paratype (Ross & Schäfer, 2000).
Plesiotrygon nana is one of the smallest Potamotrygonids known; it appears to be even smaller than Potamotrygon magdalenae and a new species of Potamotrygon from the Rio Negro basin. The holotype is a fully mature male (243 mm DL, 247 mm DW) that is probably close to the largest size reached by males of this species; females probably reach slightly larger sizes as is common in the family (in some species females are significantly larger; Araújo, 1998; Rosa et al., 2010). Males become sexually mature probably around 180 mm DL or DW but perhaps even smaller, as judged from the preadult male paratype that has well developed claspers, but which are still not fully rigid (it is probable that sexual maturity for this specimen would not have depended on additional growth). Remarkably, the smaller female, at 81 mm DL and 72 mm DW, was a free-living individual (there is no sign of an umbilical scar, just a small, dark-pigmented area), and at this size represents the smallest free living Potamotrygonid recorded (the Rio Solimões specimen depicted in Figure 10, which is slightly larger than the female paratype at 124 mm DL and 118 mm DW, was also a free-living specimen collected alone). Plesiotrygon is the only batoid genus (and probably chondrichthyan genus) in which one species reaches great sizes while its sister-group is so remarkably small in comparison; P. nana is almost three times smaller than P. iwamae, an interesting phenomenon in a family notorious for the great sizes (over 120 cm DW in some cases), attained by many of its members [e.g. Paratrygon aiereba (Müller & Henle, 1841), Heliotrygon spp., Potamotrygon brachyura].
As far as is known, Plesiotrygon nana occurs in the upper Río Amazonas basin of Peru, both in smaller tributaries and in the main Río Amazonas channel, and in the lower course of Rio Solimões in Brazil, just down-river from the mouth of Rio Purus (MZUSP 57642). The small female paratype is from Río Itaya, a small tributary of Río Nanay (near Iquitos). The male paratype was collected in Río Amazonas slightly farther south, near Tamshiyacu, and the holotype was found in Río Pachitea, a triburay of Río Ucayali near Puerto Inca (Figure 16). This species may be expected to occur in Río Napo and other regions of the upper Río Amazonas inhabited by P. iwamae. Plesiotrygon nana is not restricted to the main Amazonas channel as previously thought (accounts from the aquarium fish trade conflict).
The specific epithet nana is in reference to its dwarf size (from the Latin nanus). Gender feminine.
Dwarf antenna ray (as in aquarium literature; also known as "black-tailed" antenna ray).
Elipesurus strogylopterus: Miranda Ribeiro, 1959: 6 (non Schomburgk, 1843).
Potamotrygon scobina: Taniuchi, 1982: 27 (non Garman, 1913).
Plesiotrygon iwamae Rosa, Castello & Thorson, 1987: 447-458, Figures 1-10 (original description); Taniuchi & Ishihara, 1990: 14, 15 (claspers); Zorzi, 1995: 18 (historical account); Compagno & Cook, 1995: 67, 72, 73, 80 (brief account, compiled from Rosa et al., 1987); Carvalho, 1996: 1048 (cited); Lovejoy, 1996: 212, 216, 223-229, 233, 234 (morphology, relationships); Lovejoy et al., 1998: 1 (molecular phylogeny); Compagno, 1999: 495 (listed as valid); Carvalho et al., 2003: 23 (taxonomic account); Carvalho et al., 2004: 10, 61, 81, 88, 90 (morphology, relationships); Compagno, 2005: 540 (listed as valid); Schaefer & Summers, 2005: 303, 312 (listed, material examined); Lonardoni et al., 2006: 196 (cited); Rosa & Carvalho, 2007: 17 (listed as valid, Brazil); Luchetti et al., 2008: 131-133, 135, 140 (parasites); Toffoli et al., 2008: 325, 327, 332 (molecular phylogeny); Shibuya et al., 2009: 467, 471 (stomach contents); Domingues & Marques, 2010: 829, 832 (parasites); Ortega et al., 2010: 34 (listed, Peru); Rosa et al., 2010: 246, 247, 257, 260, 264 (general account on family).
MZUSP 10153, Rio Solimões, 03º22'S, 64º43'W, near Tefé, Amazonas, Brazil, 3 October 1981, coll. H. Britski.
FMNH 94500, Río Napo, at Anangu, Napo district, Ecuador, 00º31'36"S, 76º23'12"W, 10 October 1981, coll. D. Stewart, M. Ibarra & R. Barriga (Figures 17, 24); MNRJ 573, Rio Amazonas, Amazonas, Brazil, from Instituto Oswaldo Cruz (no further data); MZUSP 14789, Rio Solimões, 03º25'S, 60º17'W, Amazonas, Brazil, January 1977, R/V Alpha Helix; USNM 258298, Rio Solimões, Tabatinga, Amazonas, Brazil, 13 June 1969, coll. T.B. Thorson et al.; ZMH 10343, Rio Solimões, Amazonas, Brazil, 6 November 1909, coll. Scholz.
Non type material
MZUSP 42848, Baía de Marajó, Tupinambá, Pará, Brazil, coll. M. Goulding; MZUSP 59896, Rio Solimões, 03º18'S, 67º92'W, Amazonas, Brazil, 22 November 1993, coll. J.P. Friel et al.; MZUSP 59897 (same data as MZUSP 59896); MZUSP 59898, Rio Jutaí, 02º87'S, 66º93'W, Amazonas, Brazil, 16 November 1993, coll. J.G. Lundberg et al. (Figure 18); MZUSP 59899, Rio Amazonas, 03º28'S, 58º57'W, Amazonas, Brazil, 20 October 1994, coll. M. Westneat et al.; MZUSP 108706, data as in MZUSP 108767 (below) (PA 07-21); MZUSP 108707, data as in MZUSP 108767 (below) (PA 07-31); MZUSP 108767, Baía de Marajó, 00º55'34"S, 48º17'25"W, Colares, Pará, Brazil, 20 August 2007, coll. F.P.L. Marques, M. Cardoso Jr. & V.M. Bueno (PA 07-23); MZUSP 108768, data as in MZUSP 108767 (PA 07-24); MZUSP 108769, data as in MZUSP 108767 (PA 07-27); MZUSP 108770, data as in MZUSP 108767 (PA 07-28); MZUSP 108771, data as in MZUSP 108767 (PA 07-29); MZUSP 108793, data as in MZUSP 108767 (PA 07-30); MZUSP 108772, data as in MZUSP 108767, 21 August 2007 (PA 07-38); MZUSP 108773, data as in MZUSP 108767, 22 August 2007 (PA 07-39); MZUSP 108774, data as in MZUSP 108767, 22 August 2007 (PA 07-40); MZUSP 108775, data as in MZUSP 108767, 22 August 2007 (PA 07-47); MZUSP 108776, data as in MZUSP 108767, 22 August 2007 (PA 07-48); MZUSP 108790, Baía de Marajó, 00º55'34"S, 48º17'25"W, Colares, Pará, Brazil, 16 August 2007, coll. F.P.L. Marques, M. Cardoso Jr. & V.M. Bueno (PA 07-01); MZUSP 108791, Baía de Marajó, 00º55'34"S, 48º17'25"W, Colares, Pará, Brazil, 16 August 2007, coll. F.P.L. Marques, M. Cardoso Jr. & V.M. Bueno (PA 07-08); MZUSP 108792, Baía de Marajó, 00º55'34"S, 48º17'25"W, Colares, Pará, Brazil, 20 August 2007, coll. F.P.L. Marques, M. Cardoso Jr. & V.M. Bueno (PA 07-22). Plesiotrygon cf. iwamae (2 specimens): MUSM 39977, Río Amazonas, Loreto Department, Maynas Province, Sargento Lores District, town of Aucayo Caserio, near Tamshiyacu, Peru, 03º59'13.21"S, 73º10'02.80"W, altitude 89 m, 15 November 2010, coll. Homero Sanchez.
A species of Plesiotrygon, distinct from P. nana, by presenting the following characters: dorsal disc coloration with a light gray, brown or light reddish-brown background color, with numerous white or creamy-white larger blotches (close to size of spiracles) and irregular ocelli formed by smaller spots on central and posterolateral disc, with smaller, irregular spots sometimes surrounding ocelli; disc and snout markedly oval; spiracle strongly rhomboidal, with mean spiracle length 6.8% DW; snout proportionally elongate (preorbital and preoral lengths greater or equal to one-fourth DW, respectively); nasal curtain relatively wide (mouth width and internarial distance close to 10.0% DW); dorsal tail region usually with a single irregular row of enlarged spines extending to caudal stings; adult specimens with numerous tooth rows, ranging from about 40-60/42-64; distal coloration of tail, as of caudal stings, creamy white ventrally and light gray dorsally, with creamy white distal whip; relatively low number of pectoral radials, from 77-84 with a modal count of 78 (Table 4); relatively high caudal vertebrae ranging from 93-98, with a modal count of 94 (Table 4); great size, upwards of 650 mm DL or DW, reaching sexual maturity only at or over 420 mm DL or DW; external hyomandibular canal of the ventral lateral-line system with undulations at midlength, where canal bulges towards outer disc; hyomandibular canal abruptly inflected toward midline at its posterior third; and internal hyomandibular canal strongly directed toward midline posterior to gill arches. For comparisons, see also diagnosis above for Plesiotrygon nana.
Plesiotrygon iwamae has an oval disc, consistently longer than wide (DL ranging from 98.6 to 104.5% DW, mean 102.3% DW; Table 3) (for description below, refer to Figures 17-21). Anterior disc also markedly oval, with knob-like anterior protrusion always present. Anterior disc region relatively elongate, with preorbital length varying from 25.7 to 28.9% DW (mean 27.4% DW), prenasal lengths from 18.0 to 23.2% DW (mean 19.5% DW), and preoral length ranging from 22.6 to 27.9% DW (mean 25.0% DW). Eyes very small, not protruding from head, but spiracles relatively wide, rhomboidal, much greater than reduced eyes (in measured specimens, mean length of spiracles 6.8% DW, mean eye-diameter 1.8% DW; in holotype, spiracle length 8% DW, and eye-length 1.8% DW). Nasal curtain relatively wide, wider than long, with medially notched, fringed posterior margin. Junction of prenasal and supraorbital canals forming a concealed, triangular mark on nasal curtain. Rounded, tubular narial fold present. Internarial distance from 7.6 to 10.0% DW (mean 8.7% DW). Mouth also relatively wide, from 8.1 to 13.9% DW (mean 10.9% DW; 12.9% DW in holotype). Teeth set in quincunx, very small and numerous (30-60/31-64 rows, holotype with 60/64 teeth), with greater cusps in larger males. Usually five buccal papillae present inside mouth.
Pelvic fins protruding significantly from posterior disc region, somewhat triangular in dorsoventral view, and broadest posteriorly. Pelvic fin width ranging from 45.5 to 56.4% DW (mean 50.5% DW, in holotype 53.6% DW). Clasper relatively stout and short, with rounded posterior tip. Clasper groove broadly arched from apopyle to hypopyle; dorsal pseudosiphon relatively small, positioned at a slight angle (Figure 21). Ventral pseudosiphon situated on external margin of clasper tip, slightly concealed in dorsal view. Tail width at base about as wide as interspiracular distance, greatly variable in preserved material (ranging from 9.6 to 19.6% DW, mean 14.8% DW, in holotype 12.5% DW). Tail very long, greater than twice DL or DW, terminating far posteriorly as a filiform whip. Ventral median groove present, extending from tail base to beyond caudal stings posteriorly. Relatively tall ventral tail-fold originating within groove near level of caudal sting origin, tallest at more or less midlength of caudal stings (Figure 19F), and extending posteriorly for more than twice length of caudal stings. Lateral and dorsal tail folds absent. Caudal stings positioned relatively far posteriorly on dorsal tail (distance from tail base to their origin greater than one-half of disc width).
Dorsal color variable, with light gray, brown to reddish-brown background (Figure 20). Numerous large (near size of spiracles), faint, creamy white or white spots or irregular ocelli present over middisc or posterolateral disc. These spots formed by smaller speckles of white, with slightly lighter interior color. Larger, irregular spots surrounded by smaller, very faint spots. Other light irregular vermicular markings sometimes present. Some specimens with more regular, circular ocelli (e.g. MZUSP 59896; Figure 19A). Intensity of dorsal spots and ocelli vary greatly among specimens. Holotype with more uniform dark grayish-brown color, with scattered darker spots about size of eye-diameter, and smaller, white specks on outer disc. Tail base region and area of caudal stings with lateral alternating stripes of gray and creamy white. Filiform whip creamy white in color ventrally, sometimes grayish dorsally. Ventral color white to creamy white, some specimens, such as holotype, with grayish markings on posterior disc margins, pelvic fins and base of tail area. Holotype with dark filiform caudal whip.
Plesiotrygon iwamae was already known to have a relatively broad distribution when described; the type-series includes specimens from Ecuador (Río Napo) and Brazil (Rio Solimões, as far east as near Manaus). Specimens are now known from Peru in the upper Río Amazonas (e.g. near Iquitos; Ross & Schäfer, 2000; Ortega et al., 2010), as well as from Baía de Marajó, Rio Pará, their easternmost occurrence (see material examined) " a span of more than 5000 km. Based on examination of material from its entire range, however, we can advance that although there is variation, especially in dorsal coloration (Figure 20) and dorsal tail spines, consistent characters that subdivide P. iwamae into more than one species are lacking (more detailed morphological data and our results on variation in P. iwamae will be presented elsewhere). Dorsal color patterns, for example, vary even in a single locality, as demonstrated in Figure 20 (specimens depicted in B-E come from the same locality in Rio Pará). Enlarged dorsal tail spines, with very wide bases and high, acute crowns arranged in a single irregular row, are present in the vast majority of specimens of P. iwamae, but some specimens (MZUSP 59897, 108706, 108707, 108767, 108773) lack them altogether or present smaller, more scattered spines with few larger spines interspersed (Figures 19E, F, 23, 24C). Denticle morphology is similar to P. nana (Figure 22).
Two specimens of Plesiotrygon recently collected in November 2010 (MUSM 39977) from the main channel of the Río Amazonas near the town of Tamshiyacu (Peru), about 45 km upriver from Iquitos, are intriguing as they clearly cannot be identified with Plesiotrygon nana, but also differ slightly in coloration from "typical" specimens of P. iwamae (see Figures 23, 25-27). Both specimens are comparable to P. nana in size (221 mm DL and 211 mm DW, 206 mm DL and 195 mm DW; Table 5), but are juvenile males, with claspers that are just beginning to protrude beyond pelvic fin posterior web (Figure 26E). They also differ significantly in having almost 10 more tooth rows, more caudal vertebrae, much fewer total pectoral radials (see Tables 2, 6), and many proportions of disc, snout, spiracles and eyes. In contrast, these specimens share with P. iwamae similar snout and disc proportions, disc shape, nasal curtain and spiracular proportions (Table 5), tooth row counts, similar relatively low counts of pectoral radials, similar elevated counts of caudal vertebrae, creamy white color of filiform whip, conspicuous ventral lateral-line canal patterns (e.g. greatly inflected external hyomandibular canal), and a relatively stout hyomandibula (see Figures 23, 24), but differ in dorsal coloration from smaller specimens of P. iwamae (such as MZUSP 14789). However, little is known concerning the dorsal color pattern of small specimens of P. iwamae. The dorsal color pattern of the Tamshiyacu material is somewhat similar to the paratypes of P. nana, and they were collected together with the preadult male paratype of this species. Given these uncertainties, we refer to these specimens as Plesiotrygon cf. iwamae until further material from the upper Amazon can be examined; we can ascertain, though, that they are much closer morphologically to P. iwamae than to the highly distinctive P. nana. These specimens from near Tamshiyacu are illustrated in detail here to serve as a basis for future comparison because small specimens of P. iwamae are lacking in collections. The smallest captured specimen of P. iwamae we have examined is the smaller male paratype from the Rio Solimões (230 mm DL, 215 mm DW). This lack of smaller material (not counting late-term embryos aborted by captured gravid females) may be an indication that their early life-cycle after birth takes place in deeper channels.
Comparisons of Plesiotrygon nana with Plesiotrygon iwamae
Plesiotrygon nana shares with P. iwamae many generic-level characters (see also generic diagnosis above), but morphological differences between both species are considerable. In addition to the characters already mentioned as distinct in their diagnoses, some external characters deserve further notice. Plesiotrygon nana has a more intense covering of dermal denticles over disc and tail (e.g. Figure 27); dorsal disc denticles are very numerous in both male specimens, and even in the smaller female paratype (also in Solimões specimen, as from photograph). More apparent, however, is the difference in shape and size of the spiracles. In P. iwamae, the spiracles are similar to the general condition in other Potamotrygonids, being rhomboidal, and much larger than the eyes (this is true even in Potamotrygon, which has proportionally much larger eyes than in Plesiotrygon, Paratrygon and Heliotrygon). But in P. nana, the spiracles are more evenly proportioned, even oval-rounded to some degree, and much smaller proportionally (Figure 27). Plesiotrygon nana has the smallest spiracles proportionally of any Potamotrygonid. The spiracles do not even grow significantly in this species. The female paratype (81 mm DL, 72 mm DW) has spiracles measuring 5 mm in length (6.9% DW), whereas the largest specimen (holotype, 243 mm DL, 247 mm DW) has spiracles just barely greater at 7 mm in length (2.8% DW), and the preadult male paratype (174 mm DL, 170 mm DW) has spiracles almost of the same size (6 mm in length, 3.5% DW). The specimen of P. nana depicted in Figure 27A has the greatest difference between eye and spiracle length of the three specimens reported (eye-spiracle ratio, in mm, is 3/6 in this specimen, but in the holotype it is 5/7, and 4/5 in the smallest specimen). In contrast, the mean spiracular length is four times the mean eye length in specimens of P. iwamae (Table 3).
There are also slight differences in clasper shape, which in P. nana is slender, elongate and relatively flattened, but in P. iwamae is stouter, shorter and more cylindrical (Figures 9, 21). Clasper proportions, however, are not too compelling (mean external clasper length 7.7% DW, and mean internal clasper length 15.9% DW in P. nana, compared to mean external clasper length 6.6% DW, and mean internal clasper length 13.7% DW in P. iwamae), but few specimens have been examined for both species in this regard. The claspers of P. iwamae are also darker in color than in P. nana. Clasper skeleton seems to be similar in both species, judging from radiographs (we did not dissect the claspers in P. nana).
Greater distinctions between P. nana and P. iwamae are present in their ventral lateral-line canals (Figure 12). Both anterior and posterior subpleural tubules of hyomandibular canal are absent in P. nana. In P. iwamae, the anterior subpleural tubules are restricted to its dorsalmost portion right at anterior disc margin, and are few in number (some 10-15 tubules are typically present); the posterior subpleural tubule in P. iwamae extends caudally from the subpleural loop, and is very slender, easily missed in dissections. The hyomandibular canal is very narrow in P. nana, narrower than in any other Potamotrygonid. In contrast, the hyomandibular canal is relatively wide in P. iwamae, even compared to species of Potamotrygon which have a similar ventral lateral-line arrangement. Both external and internal components of the hyomandibular canal in P. iwamae are highly recurved at midlength, whereas in P. nana this canal is very straight. The slightly undulated infraorbital and supraorbital canals of P. iwamae are also distinct from the condition in P. nana, in which they are straight; species of Potamotrygon, on the other hand, have very undulated infraorbital and supraorbital canals (hypothesized to be a derived character for them). The supraorbital and infraorbital loops in P. iwamae resemble those of species of Potamotrygon in being elongated. The supraorbital loop is very long, as long as three-fourths preoral distance, and extends posteriorly past level of mouth in P. iwamae and in most species of Potamotrygon, but in P. nana it is very short, not reaching mouth posteriorly. The infraorbital loop extends posteriorly to close to the gill openings in P. iwamae and Potamotrygon spp., but in P. nana it does not come close to the gill slits (this character may be related to the shortened snout region in P. nana); the same pattern is present for the jugular canal. These characters of the ventral lateral-line have been observed in many specimens of P. iwamae through dissections as well as from external observations (and in Potamotrygon spp. as well), and in all four specimens of P. nana.
Size comparisons are a further reliable way to corroborate the separation of P. nana from P. iwamae. The smaller male paratypes of P. iwamae (MZUSP 14789, 226 mm DL, 210 mm DW; ZMH 10343, 285 mm DW; both specimens from Rio Solimões) are comparable in length to the holotype of P. nana (243 mm DL, 247 mm DW), but represent juvenile males with claspers barely noticeable, not projecting beyond pelvic fin posterior margin in the MZUSP paratype, and just extending beyond pelvic fin in the ZMH paratype (this could be related to its dried condition). The claspers in the holotype of P. nana are fully calcified and developed, and extend well beyond pelvic posterior margin. Specimens MZUSP 108772 (from Colares, Rio Pará, 299 mm DL, 295 mm DW) and MZUSP 108768 (also from Colares, Rio Pará, 344 mm DL, 349 mm DW) are much larger than the holotype of P. nana and have claspers that are very slender and barely projecting beyond pelvic fins; specimen MZUSP 108775 (again from Colares, Rio Pará, 442 mm DL, 436 mm DW) is fully sexually mature with well developed, stout claspers. Sexual maturity for P. iwamae, therefore, initiates at a much larger size than in P. nana, probably around 400 to 420 mm DL or DW, which is close to twice the size of the onset of sexual maturity for male specimens of P. nana.
Regarding skeletal morphology, the hyomandibula of P. iwamae is stouter than in P. nana, especially at its proximal one-third (Figures 14, 23, 24). Variation observed in this feature in the many specimens of P. iwamae examined is not great, but more material of P. nana is necessary. However, the pectoral and pelvic girdles of P. nana differ slightly from those of P. iwamae. Both girdles are proportionally more elongate anteroposteriorly in P. nana, as measured at their greatest length in dorsoventral view (Figures 13, 14, 23, 24). The coracoid bar in P. nana has a more pronounced concavity on anterior ventral margin (somewhat less concave and oval in P. iwamae; Figures 14, 24). Even though there is a difference of degree in scapulocoracoid length and in the anterior ventral concavity of the coracoid bar in both species of Plesiotrygon, these features may be more similar between P. nana and P. iwamae when compared to other Potamotrygonid genera. In species of Paratrygon and Heliotrygon the scapulocoracoid is anteroposteriorly shortened (Carvalho & Lovejoy, 2011), and most species of Potamotrygon have a more subtle anterior coracoid concavity; also, the scapulocoracoid is slightly less elongated (but still more similar to Plesiotrygon). But there is some variation in the anterior coracoid concavity and shoulder girdle length in species of Potamotrygon, as well as among specimens of P. iwamae examined. Nonetheless, these features reinforce the separation of P. nana and P. iwamae.
The greatest anteroposterior length of the puboischiadic bar in P. nana, measured laterally in a straight line between lateral prepelvic process and posteriormost point of iliac process, is about two-thirds the greatest width of pelvic girdle. Similar proportions occur in P. iwamae, according to our material. But in Potamotrygon, Paratrygon and Heliotrygon, the greatest anteroposterior length of the pelvic girdle is only about one-half its greatest width. In both species of Plesiotrygon, the space delimited by the posterior margin of the puboischiadic bar is more sharply oval than in the other Potamotrygonid genera as well. These features of the pelvic girdle require less additional corroboration than the greater anterior concavity of the coracoid discussed above, and represent further derived characters for Plesiotrygon.
Quo vadis Plesiotrygon?
Recent molecular phylogenetic studies have placed Plesiotrygon iwamae nested with species of Potamotrygon in approaches using neighbor joining, likelihood, and Bayesian methods (Toffoli et al., 2008). The results are highly variable, as Plesiotrygon changes position frequently among the analyses presented (Toffoli et al., 2008). What is more or less consistent, however, is that when Plesiotrygon is nested among species of Potamotrygon, it usually does so with P. schroederi from the Negro and Orinoco basins. This is similar to conclusions achieved through a more thorough molecular parsimony analysis using POY (Marques et al., in prep; F. Marques, pers. comm.), and not incompatible with the earlier molecular phylogeny of Lovejoy et al. (1998), in which Plesiotrygon iwamae is grouped with Potamotrygon orbignyi, but again from the Río Orinoco. These conclusions, if corroborated, have implications for the acceptance of Plesiotrygon as a valid Potamotrygonid genus.
In the present paper, further characters supporting the monophyly of Plesiotrygon, found in both species, were uncovered. These include their anteroposteriorly elongated puboischiadic bar, greatly concave posterior aspect of puboischiadic bar, single and uniquely stout and short angular cartilage, and greater posterior position of caudal stings on dorsal tail (this character still needs scrutiny, as some specimens of Potamotrygon scobina and P. falkneri may rival Plesiotrygon in this regard). Additionally, we can cite the posterior placement of the pelvic fins, the extremely long caudal filament, and the well developed ventral tail fold as unique among Potamotrygonids. But substantiating the monophyly of P. nana and P. iwamae together as a clade (of which there can be little doubt) will not affect conclusions as to the validity of the nominal genus Plesiotrygon (i.e. both species can be sister groups and nested within Potamotrygon, corroborating the molecular phylogenetic scenario). For Plesiotrygon to be considered valid, both species must either be basal to all Potamotrygon species, which themselves need to be monophyletic without the inclusion of P. nana and P. iwamae, or Potamotrygon would require subdivision into multiple genera to allow for separate monophyletic subgroups (of which only those species nested with the type-species, Potamotrygon histrix, would remain in Potamotrygon).
The real question, therefore, concerns not the monophyly of Plesiotrygon per se, but the monophyly of Potamotrygon without Plesiotrygon. Along these lines, we have alluded to one character above, regarding the greater undulation of the infraorbital and supraorbital lateral-line canals in species of Potamotrygon. There are other features of the lateralis system that also figure in support of Potamotrygon monophyly, but these will not be advanced here. Skeletal features of the gill arches also potentially corroborate Potamotrygon as monophyletic, along with other morphological characters. More obvious features unique for Potamotrygon within the family include its much thicker, taller head and disc (Plesiotrygon, Paratrygon and Heliotrygon have a very low head and very thin, flat disc), and bulging, much larger eyes proportionally, but these characters may be primitive when all myliobatiform genera are taken into account (e.g. Carvalho et al., 2004). In any case, this issue can only be resolved with a species-level phylogeny of all Potamotrygonid taxa; in the meantime, we see merit in recognizing Plesiotrygon as valid.
We are very grateful to Hernan Ortega and his students at the MUSM in Lima for their generosity and enormous help offered to the junior author during her visits to Lima, and for bringing material to São Paulo. Homero Sanchez is thanked for collecting the specimens from near Tamshiyacu. For discussions and technical help related to this paper, we sincerely thank João Pedro Fontenelle, João Paulo da Silva, and Diego B. Vaz from the Laboratório de Ictiologia da Universidade de São Paulo, and Murilo Carvalho from the Departamento de Biologia, FFCLRP-USP. For numerous discussions on elasmobranch systematics, the senior author thanks Ulisses L. Gomes (Universidade do Estado do Rio de Janeiro). José Lima de Figueiredo, Osvaldo T. Oyakawa and Michel Gianeti of the MZUSP are thanked for their support for registering specimens and other matters during visits to MZUSP. For assistance in collections during visits or for sending helpful information, we are grateful to Andréa Paixão and Sarah V. de Figueiredo (USP), Mary-Anne Rogers, Kevin Swagel and Mark Westneat (FMNH), Susan Jewett, Sandra Raredon, Lynne Parenti and Richard Vari (USNM), Ralf Thiel, Irina Eidus, Matthias Stehmann and Güdrun Schultze (ZMH), and Marcelo Britto (MNRJ). John G. Lundberg (Academy of Natural Sciences of Philadelphia) provided locality data, and Nathan Lovejoy (University of Toronto, Scarborough) sent photos and measurements of the Solimões specimen of P. nana. Silvana Maria Unruh, Hugo Idalgo and Reginaldo Barboza Silva (Faculdade de Medicina Veterinária e Zootecnia, USP, São Paulo). Wilson S. Júnior (USP) rendered the illustrations. Fernando P.L. Marques is sincerely thanked for obtaining the female paratype in Iquitos (field work supported by Fapesp). This project was funded by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) through grants to MRC (02/06459-0, 10/51193-5) and MPR (09/52662-1), along with financial support to MRC from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 303061/2008-1).
Araújo, M.L.G. 1998. Biologia reprodutiva e pesca de Potamotrygon sp. (Chondrichthyes, Potamotrygonidae) no médio Rio Negro, Amazonas. (Ph.D. Dissertation). Instituto Nacional de Pesquisas da Amazônia, Universidade do Amazonas, Amazonas. [ Links ]
Bigelow, H.B. & Schroeder, W.C. 1953. The fishes of the western North Atlantic, part II: sawfishes, skates, rays and chimaeroids. Memoirs Sears Foundation for Marine Research, 2:1-588. [ Links ]
Carvalho, M.R. de & Lovejoy, N.R. 2011. Morphology and phylogenetic relationships of a remarkable new genus and two new species of Neotropical freshwater stingrays from the Amazon basin (Chondrichthyes: Potamotrygonidae). Zootaxa, 2776:13-48. [ Links ]
Carvalho, M.R. de. 1996. The Biology of Freshwater Elasmobranchs: A Symposium to Honor Thomas B. Thorson. Copeia, (4):1047-1050. [ Links ]
Carvalho, M.R. de; Lovejoy, N.R. & Rosa, R.S. 2003. Family Potamotrygonidae. In: Reis, R.E; Ferraris Jr, C.J. & Kullander, S.O. (Eds.), Checklist of the Freshwater Fishes of South and Central America. EDIPUCRS, Porto Alegre, p. 22-29. [ Links ]
Carvalho, M.R. de; Maisey, J.G. & Grande, L. 2004. Freshwater stingrays of the Green River Formation of Wyoming (Early Eocene), with the description of a new genus and species and an analysis of its phylogenetic relationships (Chondrichthyes: Myliobatiformes). Bulletin of the American Museum of Natural History, 284:1-136. [ Links ]
Carvalho, M.R. de; Sabaj Perez, M.H. & Lovejoy, N.R. In press. Potamotrygon tigrina, a new species of freshwater stingray from the upper Amazon basin, closely related to Potamotrygon schroederi Fernandez-Yépez, 1958 (Chondrichthyes: Potamotrygonidae). Zootaxa. [ Links ]
Chu, Y.T. & Wen, C.M. 1979. A study of the lateral-line canals system and that of Lorenzini ampullae and tubules of Elasmobranchiate fishes of China. Shanghai Science and Technology Press, Shanghai. (Monograph of Fishes of China,2). [ Links ]
Compagno, L.J.V. & Cook, S.F.D. 1995. The exploitation and conservation of freshwater elasmobranchs: status of taxa and prospects for the future. The Biology of Freshwater Elasmobranchs. Journal of Aquariculture & Aquatic Sciences, Missouri, 7:62-90. [ Links ]
Compagno, L.J.V. & Roberts, T. 1982. Freshwater stingrays (Dasyatidae) of southeast Asia and New Guinea, with description of a new species of Himantura and reports of unidentified species. Environmental Biology of Fishes, 7(4):321-339. [ Links ]
Compagno, L.J.V. 1999. Checklist of living elasmobranches. In: Hamlett, W.C. (Ed.), Sharks, Skates and Rays, the Biology of Elasmobranch Fishes. The John Hopkins University Press, Baltimore, p. 471-498. [ Links ]
Compagno, L.J.V. 2005. Checklist of living chondrichthyes. In: Hamlett, W.C. (Ed.), Reproductive Biology and Phylogeny of Chondrichthyes: Sharks, Batoids and Chimaeras. Science Publishers, Inc. Enfield (NH), p. 503-548. [ Links ]
Dingerkus, G. & Uhler, L.D. 1977. Enzyme clearing of alcian blue stained whole smalle vertebrates for demonstration of cartilage. Stain Technology, 52(4):229-232. [ Links ]
Domingues, M.V. & Marques, T.M. 2010. Ergasilus trygonophilus sp. nov. (Copepoda: Ergasilidae) a branchial parasite of freshwater stingrays (Potamotrygonidae) from state of Pará, Brazil. Zoologia, 27(5):829-833. [ Links ]
Ewart, J.C. & Mitchell, H.C. 1892. On the lateral sense organs of elasmobranchs. II. The sensory canals of the common skate (Raja batis). Transactions of the Royal Society of Edinburgh, 37:87-105. [ Links ]
Fernandes, C.C.; Podos, J. & Lundberg, J.G. 2004. Amazonian ecology: tributaries enhance the diversity of electric fishes. Science, 305:1960-1962. [ Links ]
Garman, S. 1888. On the lateral canal system of the Selachia and Holocephala. Bulletin of the Museum of Comparative Zoology, 17:57-119. [ Links ]
Garman, S. 1913. The Plagiostomia (sharks, skates and rays). Memoirs of the Museum of Comparative Zoology, 36:1-515, 77 pls. [ Links ]
Hubbs, C.L. & Ishiyama, R. 1968. Methods for the taxonomic study and description of skates (Rajidae). Copeia, 1968(3):483-491. [ Links ]
Loboda, T.S. 2010. Revisão taxonômica e morfológica do Potamotrygon motoro (Müller & Henle, 1841) na bacia Amazônica (Chondrichthyes: Myliobatiformes: Potamotrygonidae). (Master's Dissertation). Universidade de São Paulo, São Paulo. [ Links ]
Lonardoni, A.P.; Goulart, E.; Oliveira, E.F. & Abelha, M.C.F. 2006. Hábitos alimentares e sobreposição trófica das raias Potamotrygon falkneri e Potamotrygon motoro (Chondrichthyes, Potamotrygonidae) na planície alagável do alto rio Paraná, Brasil. Acta Scientiarum. Biological Sciences, 28(3):195-202. [ Links ]
Lovejoy, N.R. 1996. Systematics of myliobatoid elasmobranchs: with enphasis on the phylogeny and historical biogeography of neotropical freshwater stingrays (Potamotrygonidae: Rajiformes). Zoological Journal of the Linnaean Society, 117:207-257. [ Links ]
Lovejoy, N.R.; Birminghan, E. & Martin, A.P. 1998. South American rays came in with the sea. Nature, 396:421-422. [ Links ]
Luchetti, N.M.; Marques, F.P.L. & Charvet-Almeida, P. 2008. A new species of Potamotrygonocestus Brooks & Thorson, 1976 (Eucestoda: Tetraphyllidea) from Plesiotrygon iwamae Rosa, Castello & Thorson (Myliobatoidea: Potamotrygonidae) and a redescription of Potamotrygonocestus chaoi Marques, Brooks & Araujo, 2003. Systematic Parasitology, 70:131-145. [ Links ]
Miranda Ribeiro, P. 1959. Catálogo dos peixes do Museu Nacional, parte 4. Museu Nacional, Rio de Janeiro, Brasil. [ Links ]
Nishida, K. 1990. Phylogeny of the suborder Myliobatidoidei. Memoirs of the Faculty of Fisheries Hokkaido University, 37(1/2):1-108. [ Links ]
Ortega, H.; Hidalgo, M.; Correa, E.; Espino, J.; Chocano, L.; Trevejo, G.; Meza, V.; Cortijo, A.M. & Quispe, R. 2010. Lista anotada de los peces de aguas continentales del Perú: estado actual del conocimiento, distribución, usos y aspectos de conservación. Ministerio del Ambiente, Dirección General de Diversidad Biológica, Museo de Historia Natural, UNMSM, Lima. [ Links ]
Rosa, R.S. & Carvalho, M.R. de. 2007. Família Potamotrygonidae. In: Buckup, P.A.; Menezes, N.A. & Ghazzi M.S. (Eds.), Catálogo de peixes de água doce do Brasil. Museu Nacional, Rio de Janeiro, p. 1-9. [ Links ]
Rosa, R.S. 1985. A systematic revision of the South American freshwater stingrays (Chondricthyes: Potamotrygonidae). (Ph.D. Dissertation). College of William and Mary, Williamsburg. [ Links ]
Rosa, R.S.; Castello, H. & Thorson, T.B. 1987. Plesiotrygon iwamae, a new genus and species of neotropical freshwater stingray. Copeia, 1987(2):447-458. [ Links ]
Rosa, R.S.; Charvet-Almeida, P. & Quijada, C.C.D. 2010. Biology of the South American Potamotrygonid stingrays. In: Carrier, J.C.; Musick, J.A. & Heithaus, M.R. (Eds.), Sharks and Their Relativies II: Biodiversity, Adaptative Physiology, and Conservation. CRC Press, Florida, p. 241-281. [ Links ]
Ross, R.A. & Schäfer, F. 2000. Aqualog Süsswasser Rochen: Freshwater Rays. Verlag ACS, Mörfelden-Walldorf. [ Links ]
Schaefer, J.T. & Summers, A.P. 2005. Batoid wing skeletal structure: Novel Morphologies, mechanical implications, and phylogenetic patterns. Journal of Morphology, 264:298-313. [ Links ]
Schomburgk, R.H. 1843. Fishes of British Guiana, Part II. In: Jardine, W. Naturalist's Library. Edinburgh, London, 40:129-214, 30 pls. [ Links ]
Shibuya, A.; Araújo, M.L.G. & Zuanon, J.A.S. 2009. Analysis of stomach contents of freshwater stingrays (Elasmobranchii, Potamotrygonidae) from the middle Negro River, Amazonas, Brazil. Pan-American Journal of Aquatic Sciences, 4(4):466-475. [ Links ]
Silva, J.P.C.B. 2010. Revisão taxonômica e morfológica do complexo Potamotrygon orbignyi (Castelnau, 1855) (Chondrichthyes: Myliobatiformes: Potamotrygonidae). (Master's Dissertation). Universidade de São Paulo, São Paulo. [ Links ]
Silva, J.P.C.B. da & Carvalho, M.R. de. 2011. A taxonomic and morphological redescription of Potamotrygon falkneri Castex & Maciel, 1963 (Chondrichthyes: Myliobatiformes: Potamotrygonidae). Neotropical Ichthyology, 9(1):209-232. [ Links ]
Stehmann, M.F.W.; McEachran, J.D. & Vergara, R. 1978. Batoids. In: Fischer, W. (Ed.), FAO Species Identification Sheets for Fishery Purposes. Western Central Atlantic (Fishing Area 31). Food and Agriculture Organization of the United Nations (FAO), Rome, v.1. (pag. var. [ Links ]).
Taniuchi, T. & Ishihara, H. 1990. Anatomical Comparison of Claspers of Freswater Stingrays (Dasyatidae and Potamotrygonidae). Japanese Journal of Ichthyology, 37(1):10-16. [ Links ]
Taniuchi, T. 1982. Investigational report of freshwater stingrays in South America. In: Mizue K. et al. (Eds.), Studies on the Adaptability and Phylogenetic Evolution of Freshwater Elasmobranchs. Scientific Research Team on Freshwater Elasmobranchs. University of Tokyo, Tokyo, p. 21-58. [ Links ]
Toffoli, D.; Hrbek, T.; Araújo, M.L.G. de; Almeida, M.P. de; Charvet-Almeida, P. & Farias, I.P. 2008. A test of the utility of DNA barcoding in the radiation of the freshwater stingray genus Potamotrygon (Potamotrygonidae, Myliobatiformes). Genetics and Molecular Biology, 31(1)(suppl):324-336. [ Links ]
Zorzi, G.D. 1995. The biology of freshwater elasmobranchs: an historical perspective. The Biology of Freshwater Elasmobranchs. Journal of Aquariculture & Aquatic Sciences, 7:10-37. [ Links ]
Recebido em: 27.02.2011
Aceito em: 04.04.2011
Impresso em: 20.04.2011