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Print version ISSN 0034-7299
Rev. Bras. Otorrinolaringol. vol.75 no.1 São Paulo Jan./Feb. 2009
Alvimar Lima de CastroI; Evanice Menezes Marçal VieiraII; Lívia Trevelin ArêdeIII; Elerson Gaetti Jardim JuniorIV
IAdjunct Professor of Stomatology - Dentistry School of Araçatuba/UNESP, Head of the Pathology and Clinical Propedeutics Department
IIPhD Student - Graduate Studies in Dentistry - Stomatology - Dentistry School of Unesp/Araçatuba, Professor - Dentistry School - Universidade de Cuiabá-UNIC/MT
IIIStudent - Faculdade de Odontologia de Araçatuba/UNESP
IVAdjunct Professor of Microbiology and Immunology - Faculdade de Odontologia de Araçatuba/UNESP, Coordinator of the MCoordenador da disciplina de microbiologia da Faculdade de Odontologia de Araçatuba/UNESP. Faculdade de Odontologia de Araçatuba - UNESP
Keywords: bone cysts, focal infection, pseudomonas, drug resistance.
The oral cavity may represent a reservoir of Pseudomonas aeruginosa, especially in patients with periodontitis; this makes treatment more difficult and, in the case of opportunistic infections, may worsen the conditions of debilitated patients, such as the elderly and immunossupressed1,2. The present investigation aims at using a clinical case to discuss the possibility of a secondary infection of a keratocystic odontogenic tumor by P. aeruginosa multiresistant to antimicrobial agents.
A 24 year-old female patient came with a panoramic radiography that showed an unerupted upper third molar associated with a radiolucent lesion (Fig. 1a), later on diagnosed as keratocystic odontogenic tumor (Fig. 1b).
Two weeks after lesion enucleation, a fistula appeared (Fig. 1c), with a buccosinusal communication and oozing of a yellowish fluid that was aspirated and sent for culture - carried out in blood agar in aerobiosis and anaerobiosis, at 37ºC, for 48 hours and 15 days, respectively. The isolated micro-organisms were identified by means of biochemical tests, showing only the presence of Pseudomonas aeruginosa.
The microorganism was submitted to antimicrobial susceptibility tests: amikacin, amoxicillin, amoxicillin/clavulanic acid, azithromycin, chloramphenicol, ciprofloxacin, clindamycin, doxicycline, erythromycin, spiramycin, streptomycin, imipenem, lincomycin, norfloxacin, G penicillin, rifampin, tetracycline, tobramycin, vancomycin, and it was resistant to all of them, with the resistance varying from 64mg/ml to 512 mg/ml, much above what could be clinically reached. The bacteria produced b-lactamase (s) capable of degrading all the b-lactamic drugs.
In the 72 hour period between clinical specimen collection and the antibiogram results, the patient was medicated with amoxicillin without satisfactory results. We then decided to terminate the use of antibiotics and we performed the surgery. The fistula remained.
In search of the factors associated with keeping the infection, by means of a radiographic analysis we identified pulpar involvement in the second upper left molar, which received endodontic treatment. Through the fistula, we removed organic remains from there using water/hydrogen peroxide (equal volumes) with satisfactory results (Fig. 1d).
Pseudomonas in the oral cavity is not a rare occurrence; however, its multiple resistance and the involvement of root canals is not common, since endodontic infections are usually associated to a mixed microbiota with a predominance of anaerobic bacteria, and Pseudomonas aeruginosa is aerobic3.
Nonetheless, in asymptomatic endodontic infections the microbiota proved to be mainly made up of facultative anaerobic and aerobic bacteria4, in such a way that the origin of this secondary infection in the keratocystic tumor is possibly associated with the dental disease. However, since we did not see any communication between the root canal system and the external environment or the presence of periodontitis, we can not safely state how the microorganism reached pulpar and periapical tissue. Nonetheless, the surgical trauma, despite all care taken with asepsis, allowed for its spread towards the tissues of the oral cavity and those of the maxillary sinus.
We must take exceptional care with the systemic spread of these multiresistant rods, and we submit that the major factor responsible for the successful treatment was the favorable health condition of the patient and the proper endodontic treatment that was undertaken. Bacteria of the Pseudomonas genus frequently have multiple resistance mechanisms, and in the case of b-lactamic, it happened because of the production of b-lactamases and, it is likely also associated with the development of water tight barriers5,6.
Pseudomonas infections of the oral cavity is not common, and the microbiologic test must be always carried out when one suspects of such infection, since because of its multiresistance, there is no drug treatment widely acknowledged as efficient. By observing the traits present in this report, we must stress that the clinician must be attentive to the possibilities of infection with uncommon characteristics in the oral cavity and the spread of such infections caused by surgical procedures.
1. Santos SSF, Loberto JCS, Martins AP, Jorge AOC. Prevalência e sensibilidade in vitro de enterobacteriaceae e Pseudomonas isoladas na cavidade bucal e bolsa periodontal de pacientes com periodontite crônica. PGRO-Pós Grad Rev Odontol 2002;5:74-84. [ Links ]
2. Bact MB, Arce R, Botero J, Mag AJ, Cruz C, Contreras A. Microorganismos inusuales em surcos y bolsas periodontales. Colomb Med 2006:6-14. [ Links ]
3. Barnes L, Eveson J, Reichart P, Sidransky D. WHO. Histological classification of odontoge-nic tumours. WHO Classification of tumours. Pathology & genetics: head and neck tumor. Lyon, France: IARCPress; 2005. [ Links ]
4. Gomes BP, Lilley JD, Drucker DB. Association of endodontic symptoms and signs with particular combinations of specific bacteria. Int Endod J 1996;29:69-75. [ Links ]
5. Guzmán-Blanco M, Casellas JM, Sader HS. Bacterial resistance to antimicrobial agents in Latin America. The giant is awakening. [ Links ]
6. Guzmán-Blanco M, Casellas JM, Sader HS. Bacterial resistance to antimicrobial agents in Latin America. The giant is awakening. Infect Dis Clin North Am 2000;14:67-81. [ Links ]
7. García PC. Resistencia bacteriana en Chile. Rev Chil Infect 2003;20:11-23. [ Links ]
Send correspondence to: This paper was submitted to the RBORL-SGP (Publishing Manager System) on 4 March 2007. code 3722
Elerson Gaetti Jardim Júnior
R. José Bonifácio, 1193
16015-050 - Caixa postal 261 - Araçatuba SP
Faculdade de Odontologia de Araçatuba - UNESP
Departamento de Patologia e Propedêutica Clínica
The article was accepted on 28 March 2007
Send correspondence to:
This paper was submitted to the RBORL-SGP (Publishing Manager System) on 4 March 2007. code 3722