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Revista Ceres

Print version ISSN 0034-737X

Rev. Ceres vol.57 no.1 Viçosa Jan./Feb. 2010

http://dx.doi.org/10.1590/S0034-737X2010000100006 

FITOSSANIDADE/PLANT PROTECTION

 

Sensibilidade de estirpes de Bradyrhizobium ao glyphosate

 

Sensitivity of Bradyrhizobium strains to glyphosate

 

 

Rodrigo Josemar Seminoti JacquesI; Sérgio de Oliveira ProcópioII; José Barbosa dos SantosIII; Maria Catarina Megumi KasuyaIV; Antônio Alberto da SilvaV

IEngenheiro-Agrônomo, Doutor. Departamento de Solos, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 971005-900, Santa Maria, Rio Grande do Sul, Brasil. rodrigosolos@yahoo.com.br
IIEngenheiro-Agrônomo, Doutor. Embrapa Tabuleiros Costeiros, Avenida Beira Mar, 3250, 49025-040, Aracaju, Sergipe, Brasil. procopio@cpatc.embrapa.br
IIIEngenheiro-Agrônomo, Doutor. Departamento de Agronomia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rodovia MGT 367, Km 583, 39100-000, Diamantina, Minas Gerais, Brasil. jbarbosa@ufvjm.edu.br
IVEngenheira-Agrônoma, Doutora. Departamento de Microbiologia, Universidade Federal de Viçosa, Avenida Peter H. Rolfs, s/n, 36570-000, Viçosa, Minas Gerais. mkasuya@ufv.br
VEngenheiro-Agrônomo, Doutor. Departamento de Fitotecnia, Universidade Federal de Viçosa, Avenida Peter H. Rolfs, s/n, 36570-000, Viçosa, Minas Gerais. aasilva@ufv.br

 

 


RESUMO

A aplicação do glyphosate sobre a soja resistente a este herbicida pode causar prejuízos à simbiose com o rizóbio. O objetivo deste trabalho foi avaliar a sensibilidade ao herbicida glyphosate de três estirpes de Bradyrhizobium recomendadas para a produção de inoculantes de sementes de soja no Brasil. Avaliou-se o efeito das concentrações de 0,0; 5,4; 10,8; 21,6 e 43,2 µg L-1 do ingrediente ativo do glyphosate [N-(fosfonometil) glicina] no meio YM líquido sobre o crescimento de B. japonicum (estirpe SEMIA 5079) e de B. elkanii (estirpe SEMIA 5019 e estirpe SEMIA 587), por meio de leituras das densidades óticas e geração de curvas de crescimento. As reduções de crescimento na presença da menor concentração do glyphosate foram de 18% para SEMIA 5079, 29% para SEMIA 5019 e de 35% para SEMIA 587, sendo, de modo geral, quanto maior a concentração do herbicida no meio de cultura maior a inibição do crescimen­to. As estirpes apresentaram sensibilidade diferencial somente às concentrações mais baixas do glyphosate; nesse caso, foi possível determinar a seguinte ordem de sensibilidade: SEMIA 587 > SEMIA 5019 > SEMIA 5079. Essa sensibilidade diferencial é dependente da concentração do herbicida, pois na presença de 43,2 µg L-1 todas as estirpes tiveram seu crescimento severamente reduzido, não havendo diferença entre elas.

Palavras-chave: Herbicida, soja, inoculante, rizóbio, EPSPs.


ABSTRACT

Glyphosate applications to glyphosate-resistant soybean may impair symbiosis with Bradyrhizobium, a symbiotic bacterium. This study evaluated the sensitivity to glyphosate of three Bradyrhizobium strains used commercially in soybean crop in Brazil. The concentrations (0.0; 5.4; 10.8; 21.6 e 43.2 µg L-1) of the active ingredient glyphosate [N­(phosphonomethyl)glycine] were added to YM liquid medium for the growth of one strain of B. japonicum (SEMIA 5079) and two strains of B. elkanni (SEMIA 5019 and SEMIA 587) inoculated as pure culture. The herbicide effect on the growth of the tested strains was assessed by optical density and growth curves were established for each strain. Reduction in growth of strains in the lowest glyphosate concentration were 18% for SEMIA 5079, 29% for SEMIA 5019 and 35% for SEMIA 587. In general, the higher the concentration of the herbicide in the culture medium, the greater the growth inhibition. The strains showed differential sensitivity only at the lowest concentrations of glyphosate, allowing the determination of the following order of sensitivity: SEMIA 587>SEMIA 5019>SEMIA 5079. This differential sensitivity is dependent on the herbicide concentration, since in the presence of 43.2 µg L-1 all strains severely reduced growth, not showing difference among them.

Key words: Herbicide, soybean, inoculant, rhizobium, EPSPs.


 

 

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Full text available only in PDF format.

 

 

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Recebido para publicação em novembro de 2008 e aprovado em setembro de 2009

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