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Adventitious rooting in cuttings of croton and hibiscus in response to indolbutyric acid and humic acid

Enraizamento adventício de estacas de cróton e hibisco em resposta à aplicação de ácido indolbutírico e ácido húmico

Abstracts

Adventitious rooting of ornamental plants can be accelerated by the application of growth regulators, such as auxin. Humic acids, organic matter in soil and organic compounds also have a biostimulant effect. This work evaluated the rooting in cuttings of croton (Codianeum variegatum L. Rumph) and hibiscus (Hibiscus rosa-sinensis L) in response to the application of different concentrations of indolbutyric acid (IBA) and humic acid (HA). The experiment was carried out in a greenhouse. Apical stem cuttings were treated with solutions at concentrations of: 0, 250, 500, 1000, 2000 mg L-1 IBA and 0, 10, 20, 30, 40 mmol L-1 HA carbon isolated from vermicomposting. Forty-five days after the applications, the cuttings were removed from the pots containing carbonized rice hull and the following variables were measured: rooting number, length and width of leaves, fresh and dry matter of root and aerial part and root area. The results were subjected to analysis of variance and the qualitative and quantitative effects of the treatments were compared by contrast and regression, respectively. Regression equations were used to determine the maximum efficiency level of root dry matter according to IBA and HA. Higher accumulation of root dry matter was recorded for the treatments with the doses 579 mg L-1 IBA and 14 mmol L-1 HA and 970 mg L-1 IBA and 50 mmol L-1 HA for root cuttings of croton and hibiscus, respectively. It was found that the application of eiher IBA or HA at the indicated doses accelerates rooting in cuttings of croton and hibiscus and contributes to the formation of vigorous plants.

Codianeum variegatum L. Rumph; Hibiscus rosa-sinensis L.; flowers; plant propagation; soil organic matter


O enraizamento adventício de plantas ornamentais pode ser acelerado por meio da aplicação de fitorreguladores de crescimento, a exemplo das auxinas. Os ácidos húmicos, a fração da matéria orgânica dos solos e os compostos orgânicos também apresentam efeito bioestimulante. O objetivo deste trabalho foi estudar o enraizamento adventício de estacas de cróton (Codianeum variegatum L. Rumph) e de hibisco (Hibiscus rosa-sinensis L.) em resposta aplicação de diferentes concentrações de ácido indolbutírico (AIB) e de ácido húmico (AH). O experimento foi realizado em casa de vegetação, sendo as estacas caulinares apicais tratadas com soluções de concentrações 0, 250, 500, 1000, 2000 mg L-1 de AIB e 0, 10, 20, 30, 40 mmol L-1 de carbono de AH isolado de vermicomposto. Aos 45 dias após a aplicação dos tratamentos, as estacas foram retiradas dos vasos contendo casca de arroz carbonizada, para medições das variáveis: pegamento; número, comprimento e largura das folhas; peso de matéria fresca e seca da raiz e da parte aérea e área radicular. Os resultados foram submetidos análise de variância e os efeitos qualitativos e quantitativos dos tratamentos foram comparados por contraste e regressão, respectivamente. As equações de regressão foram utilizadas para determinar a dose de máxima eficiência de produção de matéria seca de raiz em função das doses de AIB e AH. As doses de 579 mg L-1 de AIB e 14 mmol L-1 de C na forma de AH foram as que promoveram maior acúmulo de matéria seca no sistema radicular de estacas de cróton. Em hibiscos, as doses de 970 mg L-1 de AIB e de 50 mmol L-1 de C na forma de AH foram as que resultaram em maior acúmulo de matéria seca da raiz. Conclui-se que a aplicação de AIB ou AH nas doses indicadas acelera o processo de enraizamento adventício das estacas de cróton e hibisco, contribuindo para formação de mudas vigorosas.

Codianeum variegatum L. Rumph; Hibiscus rosa-sinensis L.; floricultura; propagação de plantas; matéria orgânica do solo


PLANT PHYSIOLOGY AND MORPHOLOGY

Adventitious rooting in cuttings of croton and hibiscus in response to indolbutyric acid and humic acid

Enraizamento adventício de estacas de cróton e hibisco em resposta à aplicação de ácido indolbutírico e ácido húmico

Lílian Estrela Borges BaldottoI; Marihus Altoé BaldottoI; Raíssa Rezende SoaresII; Herminia Emilia Prieto MartinezIII; Vitor Hugo Alvarez VenegasIV

IAgronomist engineer, DSc. Universidade Federal de Viçosa, Campus Florestal, Rodovia LMG 818, Km 06, 35690-000, Florestal, Minas Gerais, Brazil. lilian.estrela@ufv.br; marihus@ufv.br (corresponding author)

IIUndergraduate in Agronomy. Universidade Federal de Viçosa, Campus Florestal, Rodovia LMG 818, Km 06, 35690-000, Florestal, Minas Gerais, Brazil. raissa.agro@yahoo.com.br

IIIAgronomist engineer, DSc. Departamento de Fitotecnia, Universidade Federal de Viçosa, Campus Viçosa, Avenida Peter Henry Rolfs, s/n, 36570-000, Viçosa, Minas Gerais, Brazil. herminia@ufv.br

IVAgronomist engineer, DSc. Departamento de Solos, Universidade Federal de Viçosa, Campus Viçosa, Avenida Peter Henry Rolfs, s/n, 36570-000, Viçosa, Minas Gerais, Brazil. vhav@ufv.br

ABSTRACT

Adventitious rooting of ornamental plants can be accelerated by the application of growth regulators, such as auxin. Humic acids, organic matter in soil and organic compounds also have a biostimulant effect. This work evaluated the rooting in cuttings of croton (Codianeum variegatum L. Rumph) and hibiscus (Hibiscus rosa-sinensis L) in response to the application of different concentrations of indolbutyric acid (IBA) and humic acid (HA). The experiment was carried out in a greenhouse. Apical stem cuttings were treated with solutions at concentrations of: 0, 250, 500, 1000, 2000 mg L-1 IBA and 0, 10, 20, 30, 40 mmol L-1 HA carbon isolated from vermicomposting. Forty-five days after the applications, the cuttings were removed from the pots containing carbonized rice hull and the following variables were measured: rooting number, length and width of leaves, fresh and dry matter of root and aerial part and root area. The results were subjected to analysis of variance and the qualitative and quantitative effects of the treatments were compared by contrast and regression, respectively. Regression equations were used to determine the maximum efficiency level of root dry matter according to IBA and HA. Higher accumulation of root dry matter was recorded for the treatments with the doses 579 mg L-1 IBA and 14 mmol L-1 HA and 970 mg L-1 IBA and 50 mmol L-1 HA for root cuttings of croton and hibiscus, respectively. It was found that the application of eiher IBA or HA at the indicated doses accelerates rooting in cuttings of croton and hibiscus and contributes to the formation of vigorous plants.

Key words:Codianeum variegatum L. Rumph, Hibiscus rosa-sinensis L., flowers, plant propagation, soil organic matter.

RESUMO

O enraizamento adventício de plantas ornamentais pode ser acelerado por meio da aplicação de fitorreguladores de crescimento, a exemplo das auxinas. Os ácidos húmicos, a fração da matéria orgânica dos solos e os compostos orgânicos também apresentam efeito bioestimulante. O objetivo deste trabalho foi estudar o enraizamento adventício de estacas de cróton (Codianeum variegatum L. Rumph) e de hibisco (Hibiscus rosa-sinensis L.) em resposta aplicação de diferentes concentrações de ácido indolbutírico (AIB) e de ácido húmico (AH). O experimento foi realizado em casa de vegetação, sendo as estacas caulinares apicais tratadas com soluções de concentrações 0, 250, 500, 1000, 2000 mg L-1 de AIB e 0, 10, 20, 30, 40 mmol L-1 de carbono de AH isolado de vermicomposto. Aos 45 dias após a aplicação dos tratamentos, as estacas foram retiradas dos vasos contendo casca de arroz carbonizada, para medições das variáveis: pegamento; número, comprimento e largura das folhas; peso de matéria fresca e seca da raiz e da parte aérea e área radicular. Os resultados foram submetidos análise de variância e os efeitos qualitativos e quantitativos dos tratamentos foram comparados por contraste e regressão, respectivamente. As equações de regressão foram utilizadas para determinar a dose de máxima eficiência de produção de matéria seca de raiz em função das doses de AIB e AH. As doses de 579 mg L-1 de AIB e 14 mmol L-1 de C na forma de AH foram as que promoveram maior acúmulo de matéria seca no sistema radicular de estacas de cróton. Em hibiscos, as doses de 970 mg L-1 de AIB e de 50 mmol L-1 de C na forma de AH foram as que resultaram em maior acúmulo de matéria seca da raiz. Conclui-se que a aplicação de AIB ou AH nas doses indicadas acelera o processo de enraizamento adventício das estacas de cróton e hibisco, contribuindo para formação de mudas vigorosas.

Palavras-chave:Codianeum variegatum L. Rumph, Hibiscus rosa-sinensis L., floricultura, propagação de plantas, matéria orgânica do solo.

INTRODUCTION

The commercial production of flowers and ornamental plants has grown worldwide due to their high potential for income and employment generation and to the environmental benefits and life quality improvements resulting from its products (Faria, 2005; Landgraf & Paiva, 2005). The Brazilian market for flowers and ornamental plants has grown 15% in 2010 and generated 194.000 direct jobs (Ibraflor, 2011).

Among the ornamental tropical plants there are species that stand out for their lush foliage, such as croton, and for their flower production, such as hibiscus. Croton (Codianeum variegatum L. Rumph, Euphorbiaceae) is a semi-woodyshrub, 2 to 3 m in height, native to India, Malaysia and the Pacific Islands. Its showy leaves are latex producing, leathery and can have varied colors, shapes and sizes (McDonald, 2006; Lorenzi & Souza, 2008). The hibiscus (Hibiscus rosa-sinensis L., Malvaceae) is a woody shrub, originally from tropical Asia 3 to 5 m in height, with solitary flowers, usually red, pink or white-colored and blooming throughout the year (McDonald, 2006; Lorenzi & Souza, 2008).

Both species are cultivated in tropical climates and do not tolerate frost or freezing temperatures (Lorenzi & Souza, 2008). Because they are highly appreciated as ornamental plants, the propagation methods need to be fast, of low cost and must ensure the formation of vigorous, healthy plants with the desired genetic characteristics. Vegetative propagation by cuttings allows the propagated plant to retain all the genetic characteristics of the parent plant and the production of large quantities of precocious and uniform seedlings (Hartmann et al., 2011).

The adventitious rooting of cuttings involves the action of auxins, plant hormones transported to the base of the cutting that act in the formation of meristematic centers or activate pre-existing meristems that induce root formation (Hartmann et al., 2011). The indolbutyric acid (IBA) is a synthetic auxin used to promote rooting of ornamental plants on a commercial scale (Lima et al., 2008, Hartmann et al., 2011).

As an alternative, humic acids (HA), a bioactive fraction of humified organic matter (Piccolo, 2001), can be used in cuttings of ornamental plants also promoting rooting.

Humic acids comprise a set of heterogeneous organic molecules, assembled in organic aggregates, stabilized by hydrogen bonding and hydrophobic interactions (Piccolo, 2001). Humic acids can promote plant growth and development in several plants of agronomic interest (Rodda et al., 2006; Zandonadi et al., 2007; Baldotto et al., 2009). These effects reflect in the increase of root growth rate, increases in plant biomass and root architecture changes (Canellas et al., 2006; Marques Júnior et al., 2008; Baldotto et al., 2009). Stimulating effects on shoot area should also be highlighted since it increases the accumulation of foliar nutrients and chlorophyll biosynthesis (Baldotto et al., 2009).

Current studies on cellular and molecular performance of HA show the stimulation of the activity and synthesis of the enzyme H+- ATPases in the plasma membrane, which is similar to the effect produced by the auxins (Façanha et al., 2002; Canellas et al., 2006; Zandonadi et al., 2007).

This work is based on the hypothesis that the bioactive fractions of humidified organic matter in the form of HA can promote adventitious rooting in cuttings of ornamental plants and may be an alternative to commercial synthetic growth regulators, such as IBA. The objective was to evaluate rooting, sprouting and growth characteristics of stem cuttings of croton and hibiscus in response to different concentrations of IBA and HA.

MATERIALS AND METHODS

Plant material

Branches from parent plants of croton cv. 'Brasileirinho' (Codianeumvariegatum L. Rumph) and red hibiscus (Hibiscus rosa-sinensis L.) were used to obtain cuttings. The plants were removed from the campus of the Federal University of Viçosa in Florestal, MG. Softwood stem cuttings from the apical portion with 15 cm in length and four apical leaves were collected in September.

Treatments

The experimental matrix (5 x 5) consisted of the following treatments for each plant species (croton and hibiscus): five doses of indolbutyric acid (IBA) (0, 250, 500, 1000, 2000 mg L-1) and five doses of humic acids isolated from vermicompost (HA) (0, 10, 20, 30, 40 mmol L-1 carbon). Humic acids were previously isolated and characterized by Busato (2008). Cutting bases were immersed in solutions of HA for 24 hours (Baldotto et al., 2009) and in solutions of IBA for 10 seconds (Lima et al., 2008). For this procedure the cuttings were placed in plastic cups containing 50 mL of solution corresponding to the different treatments. The cuttings were transferred to pots (2.0 dm3) containing carbonized rice hulls, and kept in a greenhouse. The experimental unit consisted of pots containing five cuttings each. A randomized block design with five replications was used, totalizing 100 experimental units.

Growth analysis

Forty-five days after planting, the cuttings were collected and measured for the following variables: stem rooting (SR) regarding the number of rooted cuttings; number, length and width of leaves (NL, LL and WL), root fresh matter (RFM) and shoot fresh matter (SFM), root dry matter (RDM) and shoot dry matter (SDM), in a forced air ventilation oven at 60°C to constant mass, and root area (RA) measured after root scanning and image analysis using Delta-T SCAN ImageAnalyzer.

Statistical analysis

The results were subjected to analysis of variance, and the effects of the treatments were unfolded in mean contrasts for qualitative variables, according to Alvarez V & Alvarez (2006). For quantitative factors, regression equations were adjusted between the means of the variables and doses of IBA and HA. The F test was applied to the unfolded factors at 1, 5 and 10% probability. In the regression analysis, the slopes were assessed for determination coefficients above 0.60. The regression equations were used to establish the dose of maximum efficiency level for root dry matter production as a function of IBA and HA. To estimate this condition, the maximum efficiency doses were replaced in the regression equations of each variable.

RESULTS

Results of analysis of growth in croton and hibiscus in response to increasing concentrations of IBA and HA showed differences in root and shoot growth (Tables 1 and 2). Differences were observed among the variables, considering the mean dose of growth regulators, showing an increase when compared to the controls (Tables 1 and 2).

In cuttings of croton, the application of IBA resulted in increased SR, NL, WL, RDM, SFM, SDM, while the application of HA promoted increases in LL, RFM and RA, as shown by the means with relative increase in Table 1. However, only the variables NL, SFM and SDM significantly differed by the F test and were influenced by growth regulators, with percentage increases of 20, 15 and 25% for IBA and HA, respectively.

In cuttings of hibiscus, the application of IBA resulted in increases in all measured characteristics when compared with HA. However, only the variable SR was 13% higher (Table 2).

In general, it was possible to observe curvilinear increases in the growth characteristics of cuttings of hisbiscus and croton in response to the concentrations of IBA and AH (Figures 1 and 2, Tables 3 and 4). The response curves for increasing concentrations of growth regulators showed variation of the types quadratic, root mean square, cubic and cube root. The variable RDM was chosen to identify the dose of maximum efficiency (Figures 1 and 2). The concentrations which resulted in higher root dry matter accumulation were determined for IBA and HA, being, for croton, of 579.00 mg L-1 IBA and 14.41 mmol L-1 C in the form of AH. For this specie these doses resulted in 45.9 and 41.4 mg of RDM. For hibiscus, the higher values of RDM were obtained with 969.69 mg L-1 IBA and 49.91 mmol L-1 C in the form of HA, resulting in 194 and 49.9 mg/plant, respectively. The remaining variables were estimated using the concentration values of indolbutyric acid and humic acid determined as necessary to achieve the maximal root dry matter production of croton or hibiscus. These values were, then, used to substitute the "X" variable in regression equations showed in Tables 3 and 4 (Tables 5 and 6). In the three last columns the values found for control in each variable were compared with the values obtained for the most efficient dose of each plant regulator. Percentage differences were observed and for most of the assessed characteristics, the percentage differences were greater in hibiscus than in crotons, considering both regulators. The relative growth increases for variable RDM with the application of IBA was of 24% for croton, and 181% for hibiscus. The application of HA resulted in a relative increase of 4% for croton and 15% for hibiscus.



DISCUSSION

Vegetative propagation by cuttings allowed the production of rooted cuttings of hibiscus and croton (Tables 1 and 2). IBA was applied to accelerate seedling formation, with significant positive effects on rooting of stem cuttings of different plant species (Lima et al., 2008; Pizzatto et al., 2011, Santos et al. 2011). When applied exogenously, IBA is mainly used to act in the formation of meristematic centers, or to activate pre-existing meristems promoting the formation of root primordia and subsequent development of adventitious roots (Hartmann et al., 2011).

Several factors such as concentration, time of the year, cuttings and species affect plant response to the application of IBA (Fachinello et al., 2005; Pizzatto et al., 2011, Santos et al., 2011.) An increased concentration of exogenous IBA promotes stimulatory effect on rooting until reaching a maximum value. From this value on, growth regulators have an inhibitory effect (Fachinello et al., 2005), which was verified in the adjustment of the regression equations, which were mostly curvilinear (Figures 1 and 2, Tables 3 and 4).

Among the studied species, the hibiscus cuttings were more responsive to the application of IBA. The concentration of 970 mg L-1 promoted greatest accumulation of root dry matter (Tables 4 and 6). Pizzatto et al. (2011) also observed increases in the rooting of hibiscus cuttings, using IBA at concentration of 1600 mg L-1. The solution was applied by dipping the cuttings for only 5 seconds. In general, fast treatments tend to be more effective when combined with the highest doses (Cuquel et al., 1992).

For croton, the effects promoted by the application of growth regulators IBA or HA were not so noticeable (Table 5). Considering that rooting occurred in the absence of IBA and HA, it is possible to affirm that the endogenous levels of auxins in crotons are sufficient to stimulate the formation of adventitious roots. Therefore, this species is easily propagated by vegetative cuttings. The low effect of growth regulators may indicate a high concentration of endogenous auxin and low sensitivity of the plant tissue to the presence of the growth promoter (Trewavas & Cleland, 1983).

In a study with lettuce roots in response to application of HA isolated from vermicompost, Rodda et al. (2006) verified that HA stimulates root formation and plant growth, in the same way as IBA. Similarly, in our study, we observed that HA can also be used in propagation via stem cuttings of ornamental plants to stimulate adventitious root formation and subsequent growth of roots and shoots in cuttings of hibiscus and croton (Tables 1 and 2).

In correspondence with IBA, the effect promoted by HA depends on plant genotype and concentration of the regulator (Rodda et al., 2006; Baldotto et al., 2009). Thus, it is necessary to estimate the maximum rooting efficiency dose for each plant species (Figures 1 and 2). Other factors such as growing environment and HA source may also affect plant response to the application of growth regulators (Baldotto et al., 2011a, b). Façanha et al. (2002) found that HA isolated from vermicompost and sewage sludge promoted root growth in seedlings of corn and coffee using the energization of cell membranes by H+-pumping ATPases. Proton pumps form electrochemical gradients that maintain the secondary system of ions translocation, which is essential for nutrient absorption (Sondergaard et al., 2004). These mechanisms lead to apoplast acidification, which increases cell wall plasticity and promotes cell elongation (Rayle & Cleland, 1992). Therefore, due to the stimulation of root primordium formation and subsequent growth of adventitious roots, HA may act as an alternative to synthetic auxins in the vegetative propagation by cutting

The results indicate that IBA or HA may be used to accelerate rooting in cuttings of croton and hibiscus. This may benefit ornamental plant production and commercialization with a reduction in the time of production of propagules more adapted to field planting.

CONCLUSIONS

Humic acids are able to stimulate rooting in cuttings of croton and hibiscus and represents a technological alternative in the production of ornamentals.

The propagation of hibiscus cutting was higher with the application of 579 mg L-1 IBA or 14 mmol L-1 C HA. For the rooting of croton cuttings, we recommend the use of 970 mg L-1 IBA or 50 mmol L-1 C HA.

For both species, IBA showed a higher biostimulating effect than HA and, therefore, higher than the control.

The effect of the exogenous application of growth regulators for adventitious rooting was higher in stem cuttings of hibiscus than in cuttings of croton.

AKNOWLEDGEMENTS

The authors would like to thank FAPEMIG (APQ-02395-10) for the financial support.

Received for publication on November 23th, 2011 and approved on July 5th, 2012.

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Publication Dates

  • Publication in this collection
    27 Sept 2012
  • Date of issue
    Aug 2012

History

  • Received
    23 Nov 2011
  • Accepted
    05 July 2012
Universidade Federal de Viçosa Av. Peter Henry Rolfs, s/n, 36570-000 Viçosa, Minas Gerais Brasil, Tel./Fax: (55 31) 3612-2078 - Viçosa - MG - Brazil
E-mail: ceres@ufv.br