Gas exchanges and mineral content of corn crops irrigated with saline water¹

ABSTRACT

Inferior quality water can be used by agricultural producers in arid and semi-arid regions due to the lower availability of good-quality water. Therefore, the objective was to identify the influence of irrigation with saline water on gas exchange and leaf contents in corn (Zea mays L.) crop. The work was conducted in the field, in the experimental area of ​​the Experimental Farm of the University of International Integration of Afro-Brazilian Lusofonia (UNILAB), Redenção-CE. The experimental design used in the research was randomized blocks, with five treatments: 1.0; 2.0; 3.0; 4.0 and 5.0 dS m^-1 and four repetitions. At 45 days after sowing (DAS) the gas exchange variables were evaluated: transpiration, stomatal conductance, photosynthesis, internal CO₂ concentration, water use efficiency and leaf temperature. And at 110 DAS the contents of N, P, K, Ca and Mg in plant leaves. The saline stress due to saltwater irrigation leads to a reduction in photosynthesis, stomatal conductance, internal CO₂ concentration and transpiration in field maize plants. Salt stress reduces the levels of N, P, K, Ca and Mg mineral elements in maize leaves.

Keywords:
saline stress; gas exchange; mineral content; Zea mays L

INTRODUCTION

The corn crop is used as a food source and for energy production. It is an important source of food and income for many semi-arid producers. It is a C4 plant with high photosynthetic rates (Taiz et al. 2017), however, the photosynthetic process acts through light energy present in the leaves, acting in the formation of carbohydrates, which are allocated to the vegetative and reproductive organs (Gomes et al. 2011).

Irrigation is a technique that has contributed most to the increase in food production, however, inefficient management, often practiced by farmers, associated with climatic conditions can increase the amount of salts deposited in the soil, affecting the germination of seeds, initial plant growth and finally crop yield (Freire et al. 2018, Sousa et al. 2017; Cruz et al., 2021).

Noteworthy, salinity can causes decrease in the osmotic potential and mineral nutrition of plants (Dias et al., 2018; Sousa et al., 2021), in physiological indices (Pereira Filho et al., 2019), stomatal closure, low assimilation of CO₂, and reduces photosynthesis in thylakoid membranes of chloroplasts (Taiz et al., 2017).

Corn is considered moderately sensitive to salinity, that is, this crop has a water threshold salinity of 1.1 dS m^-1, and soil of 1.7 dS m^-1, being more sensitive to salt stress in the vegetative period and showing greater tolerance during the flowering season (Ayers & Westcot, 1999).

Assessing the initial growth of corn, Sousa et al. (2016) found that salt stress negatively affected physiological variables such as photosynthesis and sweating. In addition, Omoto et al. (2012) in corn plants, concluded that there was a reduction in gas exchange, inducing a decrease in photosynthetic metabolites due salt stress.

Saline stress will affect gas exchange and the levels of mineral elements in corn grown under field conditions. Therefore, the aim of this study was to evaluate the effect of irrigation water in different salt concentrations on gas exchange and leaf contents in maize plants.

MATERIAL AND METHODS

The experiment was carried out in the experimental area of the University of International Integration of Afro-Brazilian Lusophony (UNILAB), Redenção, Ceará. According to Alvares et al. (2013) the region's climate is of the BSh’ type, very hot temperature, with rains prevailing in the summer and autumn seasons. The region where the experiment was conducted has an average air temperature of 26 °C, an average relative humidity of 71.3% and an average annual precipitation of 1,086 mm. Rainfall during the experimental phase was 11 mm, relative humidity of 70.41% and temperature of 27.3 °C.

To evaluate the chemical analysis of the soil in the experimental area, samples were collected at a depth of 20 cm, (Table 1) following the methodology described by Texeira et al. (2017). The soil was characterized as sandy loam with density of 1.3 kg dm^-3, electrical conductivity of the saturation extract (ECse) of 0.23 dS m^-1, and hydrogenation potential (pH) = 6.

Table 1:
Chemical characterization of the soil used before maize sowing and application of treatments

Chemical attributes
OM	N	P	K+	Ca2+	Mg2+	Na+	H+ + Al3+	Al	SB	T	V
(g kg-1)		(mg kg-1)	(cmolc kg-1)								(%)
16.96	0.92	8	0.3	2.7	2.1	0.03	1.82	0.05	5.1	7	73

• OM, Organic matter; SB, sum of bases; T, cation exchange capacity; V, base saturation.

The work was developed in a randomized block experimental design, with the following treatments: five levels of salinity of the irrigation water (1.0; 2.0; 3.0; 4.0 and 5.0 dS m^-1) and four repetitions.

The spacing used during sowing (four seeds per hole) was 1.0 m between planting lines and 0.3 m between plants.

The thinning was carried out eight days after sowing (DAS), leaving one plant per hole in a total of 20 plants for each 6 meter plot, that is, presenting a planting density of 33,333 plants h^-1. In the same period, irrigations with water of different saline levels were started. During the experiment phase (10 DAS) the different saline levels were applied and calculated based on the reference evapotranspiration (ET0) and the crop coefficient (Kc) (Doorenbos & Kassam 1994), estimated by the class "A" tank method, with a two day watering shift.

The saline waters were prepared using the methodology described in Rhoades et al. (2000), that is, using the salts of NaCl, CaCl₂.2H₂O and MgCl₂.6H₂O, in 7:2:1 proportion, in non saline water (0.25 dS m^-1), obeying the relationship between the electrical conductivity of water (ECw) and its concentration (mmol_c L^-1 = CE x 10), according to Rhoades et al. (2000). Drip irrigators with a flow rate of 8 L h^-1, spaced at 0.60 m, i.e. a dripper for 2 plants, and the coefficient of uniformity of distribution (CUD) was evaluated and detected 90%.

The irrigation time was estimated under Equation 1:

$It=\frac{ETc\times Ds}{Ea\times q}\times 60$

In which: It = Irrigation time (min); ET_c = crop evapotranspiration (mm); Ds = drip spacing; Ea = Efficiency of application (0.9) and q = flow (L h^-1). A leaching fraction of 0.15 was added to the slide to be applied (Ayers & Westcot, 1999).

At 45 days after sowing (DAS), corresponding to the beginning of the reproductive phase, the following variables were evaluated: photosynthesis (A), transpiration (E), stomatal conductance (gs), CO₂ internal concentration (Ci), foliar temperature (T_f), and water use efficiency (WUE) (under the quotient (A/E)).

The measurements of the values of the physiological variables were performed using an infrared gas analyzer (LCi System, ADC, Hoddesdon, UK), between 10 am and 11 am, using an artificial radiation source (about 1,200 μmol m^-2 s ^-1).

At the end of the maize crop cycle (110 DAS), dry samples (leaf limbs) were collected and placed in paper bags, and identified as the treatments adopted. Then they were ground in a Wiley-type mill and nitrogen (N) contents determined by extracts prepared by sulfur digestion by the micro-Kjeldahl method (Tedesco et al., 1995). In order to determine the potassium (K), calcium (Ca) and magnesiumin (Mg) and phosphorus (P) content were conducted flame photometry and photocolorimetry, respectively (Malavolta et al., 1997).

Data were submitted to analysis of variance and regression, and means were compared by Tukey test with p <0.05, using the Assistat 7.7 Beta program (Silva & Azevedo 2009).

RESULTS AND DISCUSSION

According to mean square values, there was a significant influence (p≤0.01) and (p≤0.05) by the F test for all studied variables (Table 2).

Table 2:
Summary of variance analysis for the variables stomatal conductance (gs), photosynthesis (A), transpiration (E), foliar temperature (Tf), internal CO₂ concentration (Ci) and water use efficiency (WUE) in maize plants irrigated with salt water

SV	DF	Mean square
		gs	A	E	Tf	Ci	WUE
Blocks	3	0.001 ns	2.19 ns	0.031 ns	1.41 ns	60.28 ns	0.01 ns
Treatment	4	0.008*	59.37**	1.46 **	17.75**	1407.63**	4.14*
Residue	12	0.001	2.85	0.8	0.24	19.7	0.09
Total	19
GM		0.27	32.22	5.61	31.85	156.69	5.74
CV (%)		16.07	5.24	5.05	1.55	2.83	540

• SV, source of variation; DF, degree of freedom; GM, general mean; CV, coefficients of variation; ^ns, not significant; ** and * Significant at 0.01 and 0.05 by the F test, respectively.

The salt stress inhibited photosynthesis (Figure 1A), with the quadratic polynomial model being the best adjusted to the data, with maximum values of 35.6 mol m^-2 g^-1 for an electrical conductivity of the water of 2.08 dS m^-1. These effects are associated to the osmotic, toxic, and nutritional processes of saline stress, which affect the liquid assimilation of CO₂, inhibiting leaf expansion and decrease the area destined for the photosynthetic process (Taiz et al., 2017, Dias et al., 2018).

Figure 1:
Photosynthesis, transpiration, Stomatal condutctance, leaf temperature, internal CO₂ concentration and water use efficiency in maize plants irrigated with saline waters at 45 DAS.

In the same culture, Sousa et al. (2016) observed reductions in photosynthesis with increasing salt concentration in irrigation water. Similarly, Feijão et al. (2011) found that saline stress reduced photosynthesis in plants of sorghum under greenhouse conditions.

The plants showed lower values of transpiration rates with the addition of salts in the irrigation water (Figure 2B), where there were linear decreases of 23.08% of the water from high to low salinity. Corroborating information from Lima et al. (2010), where they mention that the stomatal behavior regulates the transpiration demand that the leaves are potentially subject to, controlling their loss of water to the environment, in the form of water vapor.

Figure 2:
Nitrogen, phosphorus, potassium, calcium and magnesium contents in maize leaves irrigated with saline water at 110 DAS.

Gomes et al. (2015) evaluating physiological responses in sunflower plants (Helianthus annuus L.) found that the transpiration reduced linearly as the irrigation water salinity increased. Similarly, Sousa et al. (2016) noted a reduction in the transpiration values of corn plants cultivated in a protected environment, with waters of increasing salinity.

The stomatal conductance (gs) decreased linearly with the increase in irrigation water salinity (Figure 1C), from 0.32 to 0.21 mol m^-2 s^-1, exhibiting a reduction of 0.11 mol m^-2 s^-1 between the lowest (1.0 dS m^-1) and the highest saline water treatment (5.0 dS m^-1).

To avoid excessive water loss, plants under salt stress tend to close their stomata, as there is a greater difficulty of the roots to absorb water, due to the increase of the osmotic potential (Gomes et al., 2015). Stomatal closure causes a reduction in the assimilation of CO₂ and consequently lower photosynthetic rates (Taiz et al., 2017).

Similar results were found by Sousa et al. (2016) when irrigating corn plants with potted salt water. Likewise, Gomes et al. (2011) and Omoto et al. (2012) also showed a negative effect of saline stress on stomatal conductance in corn plants.

Due to the reduction in transpiration values, as expected, the leaf temperature (T_f) increased as the concentration of the salts increased in the irrigation water, adjusting to an increasing linear model (Figure 1D). Transpiration contributes to reduce leaf temperature (cooling), which is crucial during the day when leaf is absorbing large amounts of energy from the sun (Machado et al., 2010).

The leaf temperature ranged from 30.88 °C to 32.92 °C in the low salinity water to the high salinity water respectively, i.e. there was an increase of 6.64%. The values of leaf temperature up to ECw of 3 dS m^-1 were lower than the values found by Lima et al. (2021) in peanut plants (Arachis Ypogeae, L.) irrigated with saline water. Similar results to the present study were found by Coelho et al. (2018) in 10 genotypes of sorghum irrigated with saline water.

The linear decreasing model was the best fit for the internal CO₂ concentration variable, in which the increase of the salt concentration in the water affected negatively the maize plants. The internal CO₂ concentration had a reduction of 178.52 μmol m^-2 s^-1 when low salinity water was used, and 134.85 μmol m^-2 s^-1 when water was used with salinity of 5.0 dS m^-1 (Figure 1E). Therefore, there was a reduction of 24.46%.

The partial closure of stomata causes a decrease in internal CO₂ concentration, especially when photosynthesis is maintained, even at low levels (Machado et al., 2010). Omoto et al. (2012) concluded that the NaCl solution reduced the internal CO₂ concentration in corn plants. Similar result of the present study was found by Souza et al. (2011) in cowpea (Vigna unguiculata) irrigated with saline water.

The water use efficiency responded in a polynomial quadratic manner as a function of the increase in salinity in the irrigation water (Figure 1F), where the highest value occurred in water of 3.13 dS m^-1. It can be stated that the plant saved water use in both normal and saline conditions to produce dry matter.

The answer may be related to the C4 metabolism characteristic of corn, where high rates of photosynthesis occur under conditions of low availability of CO2 caused by stomata closure (Taiz et al., 2017).

Melo et al. (2017) reported that water use efficiency increased in pepper plants after 15 days of irrigation with water of high electrical conductivity. Similarly, Coelho et al. (2018) noted that water use efficiency in 10 sorghum genotypes increased with salinity, demonstrating the crop ability to adapt to salt environment and maintain a positive photosynthesis rate, even under conditions that limit water absorption.

Table 3 shows the values of the mean squares and the significance of nutrient contents (N, P, K, Ca and Mg) found in maize leaves, according to five levels of salinity (1.0; 2.0; 3.0; 4.0 and 5.0 dS m^-1) of the irrigation water.

Table 3:
Summary of the variance analysis for the nitrogen (N), phosphorus (P) and potassium (K) variables in maize plants irrigated with salt water

	Mean square
SV	DF	N	P	K	Ca	Mg
Blocks	3	4.38ns	0.05ns	3.90ns	1.62	1.2
Treatment	4	4.35*	0.14*	37.36**	8.82**	7.32**
Residue	12	1.3	0.03	2.8	0.75	0,54
Total	19	-	-	-
MG	-	13.04	2.04	12.518	4.87	3.87
CV (%)	-	8.74	8.08	13.36	17.78	19.21

• SV, source of variation; ** and * Significant at 0.01 and 0.05 by the F test, respectively; DF, degree freedom; ns = not significant; MG, general mean; CV, coefficient of variation.

Increased salinity of irrigation water affected negatively nitrogen levels in maize leaves, with a 16.48% reduction in water from 1.0 to 5.0 dS m^-1 (Figure 2A), the linear model being the one that best fit. This response may be due to the presence of chlorine in the salts of the irrigation water causing an antagonistic effect with the nitrogen and deficiency of this element in maize plants.

Similarly, Ferreira et al. (2007) found that saline stress significantly affected nitrogen levels in leaves of corn plants at 90 and 120 DAS. In bean plants, Neves et al. (2009) also observed decreases in the nitrogen content of the leaves during the crop cycle.

In figure 2B, the phosphorus contents varied according to the quadratic polynomial model, obtaining a maximum value of 2.24 g kg^-1 for the electrical conductivity of the irrigation water of 2 dS m^-1. The ECw of 5 dS m^-1 was the most affected by the salts present in the irrigation water, leading to the deficiency of this element in the maize crop, that is, causing damages in the phosphorus functions.

Contrary to this study, Sousa et al. (2010) showed that saline stress did not influence P levels in leaf limy of corn crop. Likewise, Ferreira et al. (2007) also found a reduction in phosphorus levels in corn leaves.

Although it is a necessary element, when in excess can express characteristics detrimental to the production, such as the presence of dark-red spots on old leaves and the deficiency of cationic micronutrients such as Zn (zinc), Cu (copper), Fe (iron) and Mn (manganese), occurring by the depression of CO₂ fixation and the synthesis of starch.

In figure 2C, the linear model was the one that best fit the data, revealing that the increase of the irrigation water salinity affected negatively the potassium contents in the maize leaves, with a 44.11% reduction in water from 1.0 to 5.0 dS m^-1 thus affecting the potassium functions for the crop development.

Similarly, Sousa et al. (2010) concluded that the potassium content in corn leaves, corn grains and corn sabots was reduced with increasing saline irrigation water levels. Garcia et al. (2007) shows that soil salinity also significantly affects the potassium contents in the leaves of corn at 120 DAS, linearly decreasing its values with the increase of soil salinity levels.

As seen in figure 2D, the Ca content was negatively affected by the irrigation water. When irrigated with water of 5 dS m^-1, the Ca content decreased by 54.81% in relation to the treatment with water of 1 dS m^-1. Although calcium chloride has been used in water, its proportion in relation to sodium chloride is much lower, however, the presence of sodium may have affected the bioavailability of calcium.

Similarly, Song et al. (2017) working with perennial ryegrass accessions under salinity stress, found a reduction in the concentration of Ca⁺ in the treatment with high salinity. On the other hand, Coelho et al. (2017), when studying the accumulation of nutrients in forage sorghum genotypes under salinity, found an increase in the Ca⁺ leaf content.

The Mg values were adjusted to the quadratic polynomial model (Figure 2E), with a maximum value of 2.21 g kg^-1, for an ECw of 2.56 dS m^-1. Possibly, the decrease in the leaf content of Mg is related to an inhibition of the biological functions of the roots, caused by the high concentration of salts in the irrigation water (Ahmadi & Souri, 2018).

In the cowpea culture, Sá et al. (2021) also observed a quadratic behavior in the concentration of Mg in the area, noting an increase up to the electrical conductivity of the water of 1.34 and 1.10 dS m^-1, with subsequent reductions up to the value of 4.5 dS m^-1.

CONCLUSIONS

The saline stress due to saltwater irrigation leads to a reduction in photosynthesis, stomatal conductance, internal CO₂ concentration and transpiration in field maize plants.

Foliar temperature and water use efficiency increased as a result of increased salinity levels in irrigation water, but in the case of water use efficiency this increase occurred until the electrical conductivity of the water of 4 ds m^-1.

Salt stress reduces the levels of N, P, K, Ca and Mg mineral elements in maize leaves.

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1

• 1
  This work is part of the first author's master's dissertation, and it was funded by Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP).

Publication Dates

• Publication in this collection
  08 Nov 2021
• Date of issue
  Sep-Oct 2021

History

• Received
  11 Sept 2019
• Accepted
  15 Apr 2021