Depressão humoral específica em pacientes crônicos infectados pelo Trypanosoma cruzi

Brénière, Simone Frédérique; Poch, Olivier; Selaes, Hugo; Tibayrenc, Michel; Lemesre, Jean-loup; Antezana, Gerardo; Desjeux, Philippe

doi:10.1590/S0036-46651984000500005

Resumos

Realizamos um estudo comparativo entre o xenodiagnóstico e os testes sorológicos para a doença de Chagas. Cento e cincoenta pacientes de algumas áreas endêmicas foram estudados. Quatro deles pareceram revelar um estado particular com um xenodiagnóstico positivo e uma sorologia negativa, esta realizada com quatro diferentes técnicas clássicas (teste de immunofluorescência, ELISA: Enzyme Linked Immunosorbent Assay, teste de fixação do complemento e teste de immuno-eletroforese). O soro de um dos pacientes que apresentou depressão humoral específica mostra elevada quantidade de antígenos circulantes comprovada pela técnica da immuno-eletroforese. Os Autores sugerem o uso de um teste sorológico para detectar a presença de antígenos circulantes de T. cruzi, além da utilização de testes sorológicos clássicos. Isto permitiria o diagnóstico da doença de Chagas em pacientes com uma baixa (ou mesmo inexistente) produção de anticorpos específicos.

We performed a comparative study between xenodiagnosis and serological tests for Chagas'disease. 150 Patients from several endemic areas were studied. Pour of them appeared to have a peculiar status with positive xenodiagnosis and negative serology carried out with four classical techniques (Immunofluorescence test, ELISA: Enzyme Linked Immunosorbent Assay, Complement fixation test and Immunoelectrophoresis). One serum out of the four patients presenting humoral depression showed a high quantity of circulating antigen proved by Immunoelectrophoresis. The Authors suggest the use of one serological test for detecting circulating antigens of Trypanosoma cruzi in addition to the classical serology. It would allow the diagnosis of Chagas' disease in patients with low production of specific antibodies.

Specific humoral depression in chronic patients infected by Trypanosoma cruzi

Depressão humoral específica em pacientes crônicos infectados pelo Trypanosoma cruzi

Simone Frédérique Brénière^I; Olivier Poch^II; Hugo Selaes^III; Michel Tibayrenc^I; Jean-loup Lemesre^IV; Gerardo Antezana^V; Philippe Desjeux^VI

^IORSTOM, IBBA, C/o Embajada de Francia, Casilla 824, La Paz, Bolivia

^IIUniversité Louis Pasteur de Strasbourg, IBBA, C/o Embajada de Francia, Casilla 824, La Paz, Bolivia

^IIIINLASA, Pasaje Zubieta, Miraflores, La Paz, Bolivia

^IVCIBP, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille Cédex, France

^VIBBA, C/o Embajada d'e Francia, Casilla 824, La Paz, Bolivia

^VIInstitut Pasteur de Paris, IBBA, C/o Embajada de Francia, Casilla 824, La Paz, Bolivia

SUMMARY

We performed a comparative study between xenodiagnosis and serological tests for Chagas'disease. 150 Patients from several endemic areas were studied. Pour of them appeared to have a peculiar status with positive xenodiagnosis and negative serology carried out with four classical techniques (Immunofluorescence test, ELISA: Enzyme Linked Immunosorbent Assay, Complement fixation test and Immunoelectrophoresis). One serum out of the four patients presenting humoral depression showed a high quantity of circulating antigen proved by Immunoelectrophoresis. The Authors suggest the use of one serological test for detecting circulating antigens of Trypanosoma cruzi in addition to the classical serology. It would allow the diagnosis of Chagas' disease in patients with low production of specific antibodies.

RESUMO

Realizamos um estudo comparativo entre o xenodiagnóstico e os testes sorológicos para a doença de Chagas. Cento e cincoenta pacientes de algumas áreas endêmicas foram estudados. Quatro deles pareceram revelar um estado particular com um xenodiagnóstico positivo e uma sorologia negativa, esta realizada com quatro diferentes técnicas clássicas (teste de immunofluorescência, ELISA: Enzyme Linked Immunosorbent Assay, teste de fixação do complemento e teste de immuno-eletroforese).

O soro de um dos pacientes que apresentou depressão humoral específica mostra elevada quantidade de antígenos circulantes comprovada pela técnica da immuno-eletroforese. Os Autores sugerem o uso de um teste sorológico para detectar a presença de antígenos circulantes de T. cruzi, além da utilização de testes sorológicos clássicos. Isto permitiria o diagnóstico da doença de Chagas em pacientes com uma baixa (ou mesmo inexistente) produção de anticorpos específicos.

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ACKNOWLEDGEMENTS

This work was supported by a grant of the French Cooperation Ministry and the French Industry and Research Ministry (PVD/81/L-1423).

Recebido para publicação em 15/4/1983.

1 AFCHAIN, D.; CAPRON, A. & PRATA, A. Les anti-corps précipitants dans la Trypanosomiase humaine. Gaz. Med. Bahia 5: 141-147, 1970.
2. ARAÚJO, F. G.; CHIARI, E. & DIAS, J. C. P. Demonstration of Trypanosoma cruzi antigen in serum from patients with Chagas'disease. Lancet 8214: 246249, 1981.
3. BARCLAY, C. A.; CERISOLA, J. A.; LUGONES, H.; LEDESMA, 0.; LOPEZ SILVA, J.; MOUZO, G. & SIERRA, J. P. Resultados de la actividad anti-T. cruzi del Benznidazole en el hombre. VI Congreso Latino-americano de Farmacologia, Buenos Aires 2: 9-12, 1976.
4. BOUT, D.; DUGIMONT, J. C; FARAG, H. & CAPRON, A. Immunodiagnosis of human parasitic diseases by the Enzyme Linked Immunosorbent Assay. First International Symposium on Immunoenzymatic techniques, INSERM Symposium, Ed. Felman et al. Amsterdam, North-Holland Publishing Company, 1975, 175182.
5. CAMARGO, M. E. & TAKEDA, G. K. F. Diagnóstico de laboratorio. In: BRENER, Z. & ANDRADE, Z., ed. Trypanosoma cruzi e Doença de Chagas. 1st (ed.). Rio de Janeiro, Guanabara, 1979, 175-198.
6. CAPRON, A.; CAMUS, D.; DESSAINT, J. P. & BOU-BENNEC-FISHER, F. Alterations de la réponse immune au cours des infections parasitaires. Ann. d'Immunologie (Institut Pasteur) 128C: 541-556, 1977.
7. COURA, J. R.; BRINDERIO, P. J. & FERREIRA, I. Benznidazole in the treatment of Chagas'disease. Current chemotherapy. Proceeding of the 10th Intern. Congress of Chemotherapy. Zurich, Switzerland, 18-23 Sept. 1977, 1: 161-162, Am. Soc. Microbiol., Washington D.C., 1978.
8. COX, F. E. G. Enhanced Trypanosoma musculi infections in mice with concomitant malaria. Nature (London) 258: 148-149, 1975.
9. GUERREIRO, C. & MACHADO, A. Da reação de Bordet e Gengou na molestia de Carlos Chagas como elemento de diagnóstico. Brazil Méd. 27: 223-226, 1913.
10. KENT, J. F. ,& FIFE, E .H. Precise estandardiza-tion of reagent for complement fixation. Am. J. Trop. Med. Hyg. 12: 103-116, 1963.
11. KIERSZENBAUM, F. On evasion of Trypanosoma cruzi from the host immune response. Lymphoproli-ferative responses to trypanosomal antigens during acute and chronic experimental Chagas'disease. Immunology 44: 641-648, 1981.
12. LELCHUK, R.; DALMASSO, A. P.; INGLESINI, C. L.: ALVAREZ, M. & CERISOLA, J. A. Immunoglobulin studies in serum of patients with American Trypanosomiasis (Chagas'disease). Clin. Exp. Immunol. 6: 548-555, 1970.
13. LEMESRE, J. L. Specific antigen 5 of Trypanosoma cruzi: partial purification and diagnosis application. Molecular and Biochemical Parasitology. OCIPA V, Toronto 7-14 August, (Suppl.) p. 668, 1982.
14. SALAMAN, M. H. Immunodepression by viruses. Antibiotics & Chemother. 15: 393-406, 1969.
15. SCHWAB, J. H. Suppression of the immune response by micro-organisms. Bact. Rev. 39: 121-143, 1975.
16. TEIXEIRA, A. R. L.; TEIXEIRA, G.; MACEDO, V. & PRATA, A. Acquired cell mediated immunodepression in acute Chagas'disease. J. Clin. Invest. 62: 11321141, 1978.
17. TERRY, R. J. Immunodepression in parasite infections. INSERM 72: 161-178, 1977.
18. TIBAYRENC, M.; ECHALAR, L. & DESJEUX, P. Une méthode simple pour obtenir directement des isolates de Trypanosoma cruzi à partir du tube digestif du triatome vecteur. Can. ORSTOM Sér. Ent. méd. et Parasitol. XX: 187-188, 1982.
19. TIBAYRENC, M.; ECHALAR, L.; LE PONT, F. & DESJEUX, P. Presence en Bolivie de sept nouveaux variants isoenzymatiques de Trypanosoma cruzi Considerations taxonomiques et émidémiologiques. Discussion sur la valeur antigénique potentielle de certames isoenzymes. Cah. ORSTOM, Sér. Ent. méd. et Parasitol. (sous presse).
20. TSCHUDI, E. I.; ANZIANO, D. F. & DALMASSO, A. P. Lymphocyte transformation in Chagas'disease. Infect. Immunity 6: 905-908, 1972.
21. VOLLER, A.; DRAPER, C. ; BIDWELL, D. E. & BAR-TLETT, A. Microplate Enzyme Linked! Immunosorbent Assay for Chagas disease. Lancet 1: 426-428, 1975.
22. WELLER, T. H. & COONS, A. H. Fluorescent antibodies studies with agents of Varicella and Herpes Zoster propagated in vitro. Proc. Soc. Exp. Biol. (N.Y.) 86: 789-794, 1954.

Datas de Publicação

Publicação nesta coleção
08 Abr 2013
Data do Fascículo
Out 1984

Histórico

Recebido
15 Abr 1983

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1 AFCHAIN, D.; CAPRON, A. & PRATA, A. Les anti-corps précipitants dans la Trypanosomiase humaine. Gaz. Med. Bahia 5: 141-147, 1970.

[2] 2. ARAÚJO, F. G.; CHIARI, E. & DIAS, J. C. P. Demonstration of Trypanosoma cruzi antigen in serum from patients with Chagas'disease. Lancet 8214: 246249, 1981.

[3] 3. BARCLAY, C. A.; CERISOLA, J. A.; LUGONES, H.; LEDESMA, 0.; LOPEZ SILVA, J.; MOUZO, G. & SIERRA, J. P. Resultados de la actividad anti-T. cruzi del Benznidazole en el hombre. VI Congreso Latino-americano de Farmacologia, Buenos Aires 2: 9-12, 1976.

[4] 4. BOUT, D.; DUGIMONT, J. C; FARAG, H. & CAPRON, A. Immunodiagnosis of human parasitic diseases by the Enzyme Linked Immunosorbent Assay. First International Symposium on Immunoenzymatic techniques, INSERM Symposium, Ed. Felman et al. Amsterdam, North-Holland Publishing Company, 1975, 175182.

[5] 5. CAMARGO, M. E. & TAKEDA, G. K. F. Diagnóstico de laboratorio. In: BRENER, Z. & ANDRADE, Z., ed. Trypanosoma cruzi e Doença de Chagas. 1st (ed.). Rio de Janeiro, Guanabara, 1979, 175-198.

[6] 6. CAPRON, A.; CAMUS, D.; DESSAINT, J. P. & BOU-BENNEC-FISHER, F. Alterations de la réponse immune au cours des infections parasitaires. Ann. d'Immunologie (Institut Pasteur) 128C: 541-556, 1977.

[7] 7. COURA, J. R.; BRINDERIO, P. J. & FERREIRA, I. Benznidazole in the treatment of Chagas'disease. Current chemotherapy. Proceeding of the 10th Intern. Congress of Chemotherapy. Zurich, Switzerland, 18-23 Sept. 1977, 1: 161-162, Am. Soc. Microbiol., Washington D.C., 1978.

[8] 8. COX, F. E. G. Enhanced Trypanosoma musculi infections in mice with concomitant malaria. Nature (London) 258: 148-149, 1975.

[9] 9. GUERREIRO, C. & MACHADO, A. Da reação de Bordet e Gengou na molestia de Carlos Chagas como elemento de diagnóstico. Brazil Méd. 27: 223-226, 1913.

[10] 10. KENT, J. F. ,& FIFE, E .H. Precise estandardiza-tion of reagent for complement fixation. Am. J. Trop. Med. Hyg. 12: 103-116, 1963.

[11] 11. KIERSZENBAUM, F. On evasion of Trypanosoma cruzi from the host immune response. Lymphoproli-ferative responses to trypanosomal antigens during acute and chronic experimental Chagas'disease. Immunology 44: 641-648, 1981.

[12] 12. LELCHUK, R.; DALMASSO, A. P.; INGLESINI, C. L.: ALVAREZ, M. & CERISOLA, J. A. Immunoglobulin studies in serum of patients with American Trypanosomiasis (Chagas'disease). Clin. Exp. Immunol. 6: 548-555, 1970.

[13] 13. LEMESRE, J. L. Specific antigen 5 of Trypanosoma cruzi: partial purification and diagnosis application. Molecular and Biochemical Parasitology. OCIPA V, Toronto 7-14 August, (Suppl.) p. 668, 1982.

[14] 14. SALAMAN, M. H. Immunodepression by viruses. Antibiotics & Chemother. 15: 393-406, 1969.

[15] 15. SCHWAB, J. H. Suppression of the immune response by micro-organisms. Bact. Rev. 39: 121-143, 1975.

[16] 16. TEIXEIRA, A. R. L.; TEIXEIRA, G.; MACEDO, V. & PRATA, A. Acquired cell mediated immunodepression in acute Chagas'disease. J. Clin. Invest. 62: 11321141, 1978.

[17] 17. TERRY, R. J. Immunodepression in parasite infections. INSERM 72: 161-178, 1977.

[18] 18. TIBAYRENC, M.; ECHALAR, L. & DESJEUX, P. Une méthode simple pour obtenir directement des isolates de Trypanosoma cruzi à partir du tube digestif du triatome vecteur. Can. ORSTOM Sér. Ent. méd. et Parasitol. XX: 187-188, 1982.

[19] 19. TIBAYRENC, M.; ECHALAR, L.; LE PONT, F. & DESJEUX, P. Presence en Bolivie de sept nouveaux variants isoenzymatiques de Trypanosoma cruzi Considerations taxonomiques et émidémiologiques. Discussion sur la valeur antigénique potentielle de certames isoenzymes. Cah. ORSTOM, Sér. Ent. méd. et Parasitol. (sous presse).

[20] 20. TSCHUDI, E. I.; ANZIANO, D. F. & DALMASSO, A. P. Lymphocyte transformation in Chagas'disease. Infect. Immunity 6: 905-908, 1972.

[21] 21. VOLLER, A.; DRAPER, C. ; BIDWELL, D. E. & BAR-TLETT, A. Microplate Enzyme Linked! Immunosorbent Assay for Chagas disease. Lancet 1: 426-428, 1975.

[22] 22. WELLER, T. H. & COONS, A. H. Fluorescent antibodies studies with agents of Varicella and Herpes Zoster propagated in vitro. Proc. Soc. Exp. Biol. (N.Y.) 86: 789-794, 1954.

Brasil

Brasil

Depressão humoral específica em pacientes crônicos infectados pelo Trypanosoma cruzi

Specific humoral depression in chronic patients infected by Trypanosoma cruzi

Resumos

Datas de Publicação

Histórico