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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.26 no.6 São Paulo Nov./Dec. 1984

https://doi.org/10.1590/S0036-46651984000600002 

Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence

 

Detecção de E. coli enteropatogênica clássica e invasora e Shigella em fezes por imunofluorescência indireta

 

 

Waldely de Oliveira Dias; Octávio Augusto de Carvalho Pereira

Escola Paulista de Medicina. Disciplina de Imunologia. Departamento de Microbiologia, Imunologia e Parasitologia Rua Botuoatu, 862 — 4.° andar. Caixa Postal 7.144 São Paulo, SP — Brasil

 

 


SUMMARY

IIF in the detection of invasive and classic enteropathogenic E. coli and Shigella serotypes was compared with traditional coproculture methods. IIP results agreed with the coproculture findings in 128 out of 140 cases tested for enteropathogenic E. coli (91%) and in 108 out of 112 for Shigella (96%). All cases with positive reactions by coproculture were confirmed by IIP. In the control group it were obtained by IIF 12 cases with positive reactions for enteropathogenic E. coli and 4 cases for Shigella, including two cases of mixed infection by E. coli 026/Sh. dysenteriae and E. coli 0124/Sh. dysenteriae. It was discussed the high sensitivity and specificity of the IIF when compared with the traditional methods, being suggested that IIF is a valuable tool in epidemiological studies involving these organisms and an important aid in the stablishment of an early presumptive diagnosis of the acute infantile diarrhea.


RESUMO

A imunofluorescência indireta (IFI) de sorotipos enteropatogênicos clássicos e invasores de E. coli e de Shigella foi comparada com os métodos tradicionais de coprocultura e soroaglutinação. Os resultados da IFI concordaram com os da coprocultura em 128 dos 140 casos testados para E. coli enteropatogênica (91%) e em 108 dos 112 testados para Shigella (96%). Todos os casos com reações positivas por coprocultura foram confirmados por IFI. No grupo controle, onde não haviam sido isolados tais patógenos por coprocultura, foram evidenciados por IFI, 12 casos com reações positivas para E. coli enteropatogênica e 4 para Shigella, incluindo-se 2 com infecção mista: E. coli 026/Sh. dysenteriae e E. coli 0124/Sh. dysenteriae. Foi discutida a alta sensibilidade e especificidade da IFI quando comparada aos métodos tradicionais, sendo sugerido o valor desta técnica em estudos epidemiológicos envolvendo os microrganismos em questão e sua importância no estabelecimento de diagnóstico precoce na diarréia infantil aguda.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

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Recebido para publicação em 10/2/1984.

 

 

Este trabalho recebeu auxílio financeiro da FAPESP e CNPq

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