Strongyloses ratti and S. stercoralis: effects of cambendazole, thiabendazole and mebendazole in vitro

Grove, David I.; Northern, Carolyn

doi:10.1590/S0036-46651986000200006

Abstracts

The effects of in vitro incubation of three henzimidazole anthelmintics, thiabendazole, mebendazole and cambendazole on Strongyloides were compared. No drug affected hatching of S. ratti eggs or the viability of infective larvae or parasitic adult worms, but all three inhibited moulting of S. ratti larvae. In addition, cambendazole, but not thiabendazole or mebendazole, impaired the viability of S. ratti first- and second-stage larvae. The three drugs had no effect on isolated S. stercorais free-living adult worms, but they all prevented development of S. stercoralis rhabditiform larvae. Thiabendazole and mebendazole had no effect on the infectivity of either S. ratti or S. stercoralis infective larvae, but infection with these worms was abrogated by prior incubation with cambendazole. These results indicate that cambendazole acts in a different manner to the other two drugs. Since it is active against larvae migrating through the tissues, it is potentially of much greater value than thiabendazole or mebendazole in the therapy of strongyloidiasis.

Strongyloides ratti; Strongyloides stercoralis; Action in vitro drugs; Cambendazole; Thiabendazole; Mebendazole

Os efeitos da incubação de três antihelmínticos, tiabendazol, mebendazol e cambendazol sobre Strongyloides foram comparados. Nenhuma droga afetou a eclosão dos ovos de S. ratti ou a viabilidade de larvas infectantes ou vermes adultos parasitários, mas todas as três inibiram a formação de larvas de S. ratti. Além disso, cambendazol, mas não tiabendazol ou mebendazol, diminuiu a viabilidade de larvas de primeiro e segundo estágio de S. ratti. As três drogas não tiveram efeito sobre vermes adultos de vida livre isolados, de S. stercoralis, mas todas evitaram o desenvolvimento de larvas rabditiformes de S. stercoralis. Tiabenda zol e mebendazol não tiveram efeito sobre a infectividade de larvas infectantes de S. ratti ou de S. stercoralis, mas a infecção com esses vermes foi anulada por incubação prévia com cambendazol. Esses resultados indicam que cambendazol age de modo diferente das outras duas drogas. Uma vez que ele é ativo contra lar vas migrando através dos tecidos, é potencial mente de muito maior valor que o tia bendazol ou mebendazol na terapêutica da esbrongiloidíase.

THERAPEUTIC ASSAYS

Strongyloses ratti and S. stercoralis: effects of cambendazole, thiabendazole and mebendazole in vitro

Strongyloses ratti e S. stercoralis: efeitos de cambendazol, tiabendazol e mebendazol in vitro

David I. Grove; Carolyn Northern

Department of Medicine, University of Western Australia, Queen Elizabeth It Medical Centre, Neulands, Western Australia. 6009

^{Correspondence} Correspondence: Dr. D. I. Grove Department of Medicine University of Western Australia Queen Elizabeth II Medical Centre Nedlands, Western Australia, 6009

SUMMARY

The effects of in vitro incubation of three henzimidazole anthelmintics, thiabendazole, mebendazole and cambendazole on Strongyloides were compared. No drug affected hatching of S. ratti eggs or the viability of infective larvae or parasitic adult worms, but all three inhibited moulting of S. ratti larvae. In addition, cambendazole, but not thiabendazole or mebendazole, impaired the viability of S. ratti first- and second-stage larvae. The three drugs had no effect on isolated S. stercorais free-living adult worms, but they all prevented development of S. stercoralis rhabditiform larvae. Thiabendazole and mebendazole had no effect on the infectivity of either S. ratti or S. stercoralis infective larvae, but infection with these worms was abrogated by prior incubation with cambendazole. These results indicate that cambendazole acts in a different manner to the other two drugs. Since it is active against larvae migrating through the tissues, it is potentially of much greater value than thiabendazole or mebendazole in the therapy of strongyloidiasis.

Key words:Strongyloides ratti Strongyloides stercoralis Action in vitro drugs Cambendazole Thiabendazole Mebendazole.

RESUMO

Os efeitos da incubação de três antihelmínticos, tiabendazol, mebendazol e cambendazol sobre Strongyloides foram comparados. Nenhuma droga afetou a eclosão dos ovos de S. ratti ou a viabilidade de larvas infectantes ou vermes adultos parasitários, mas todas as três inibiram a formação de larvas de S. ratti. Além disso, cambendazol, mas não tiabendazol ou mebendazol, diminuiu a viabilidade de larvas de primeiro e segundo estágio de S. ratti. As três drogas não tiveram efeito sobre vermes adultos de vida livre isolados, de S. stercoralis, mas todas evitaram o desenvolvimento de larvas rabditiformes de S. stercoralis. Tiabenda zol e mebendazol não tiveram efeito sobre a infectividade de larvas infectantes de S. ratti ou de S. stercoralis, mas a infecção com esses vermes foi anulada por incubação prévia com cambendazol. Esses resultados indicam que cambendazol age de modo diferente das outras duas drogas. Uma vez que ele é ativo contra lar vas migrando através dos tecidos, é potencial mente de muito maior valor que o tia bendazol ou mebendazol na terapêutica da esbrongiloidíase.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

ACKNOWLEDGEMENT

This study was supported by a grant from the National Health and Medical Research Council of Australia.

4. CHONGSUPHAJAISIDDHI, T.; SABCAROEN, A.; ATTANATH, P.; PANASOPONKUL, C. & RADOMYOS, P. Treatment of soil-transmitted nematode infections in children with mebendazole. Ann. trop. Med. Parasit., 7.3: 59-63, 1978.

Recebido para publicação em 22/2/1985.

1. AMATO NETO, V.; SINTO, T.; PEDRO, R. J.; LEVI, G. C. ; TSUKUMO, M. K.; MORAES, U. M. & CORREA, L. L. Nossas observações iniciais sobre a eficácia do cambendazole no tratamento da estrongiloidíase. Rev. Inst. Med. trop. S. Paulo, 20: 161-163, 1978.
2. BARANSKI, M. C; SILVA, A. P.; KOTAKA, P. I.; GOMES, N. R. ; GIOVANNI, M. & TELLES, J. E. Tratamento da, estrongiloidíase humana com nova droga anti-heimíntica, o cambendazole. Estudo duplo cego, Rev. Inst. Med. trop, S. Paulo, 20: 213-218, 1978.
3. BICALHO, S. A.; LEÃO, O. J. & PENA Jr., Q. Cambendazole in the treatment of human Strongyloidiasis. Amer. J. trop. Med. Hyg., 32: 1181-1183, 1983.
5. DAWKINS, H. J.; GEOVE, D. I.; DUNSMORE, J. E. & MITCHELL, G. F. Strongyloides ratti: susceptibility to infection and resistance to reinfection as assessed by excretion of larvae. Int. J. Parasit., 10: 125-129, 1380.
6. DESCHIENS, R. & BENEX, J. Essais expérimentaux ét cliniques de traitement de l'anguilullose intestinale par les sous-nitrate et par le carbonate de bismuth. Bull. Soc. Path. exot., 50: 70-74, 1957.
7. GROVE, D. I. Strongyloidiasis in Allied ex-prisoners of war in southeast Asia. Brit. med. J., 280: 598-601, 1980.
8. GROVE, D. I. Treatment of strongyloidiasis: an analysis of toxicity and. effectiveness. Trans. roy. Soc. trop. Med. Hyg., 76: 114-116, 1982.
9. GROVE, D. I. Strongyloides rattf and S. stereorails: the effects of thiabendazole, mebendazole and cambendazole in infected mice. Amer. J. trop. Med. Hyg., 31: 469-476, 1982.
10. GROVE, D. I. The effects of 22, 23-dihydroavermectin B₁ on Strongyloides ratti and S. stercoralis infections in mice. Ann. trop. Med. Parasit., 77: 405-410, 1983.
11. GROVE. D. I. & NORTHERN, C. Infection and immunity in dogs infected with a human strain of Strongyloides stercoralis. Trans. roy. Soc. trop. Med. Hyg., 76: 833-838, 1982.
12. HOFF, D. R.; FISHER, M. H.; BOCHIS, R. J.; LUSI, R. J.; WAKSMUNSKI, A.; EGERTON, R. J.; YASTIS, J. J.; CUCKLER, A. C. & CAMPBELL, W. C. A new broad spectrum anthelmintic: 2-(4-thiazolyl)-iso- propoxycarbonyl aminobenzimidazole. Experientia, 26: 550-552, 1970.
13. HUGGINS, D. Cambendazole-nova substância para o tratamento da estrongiloidíase. GEN, 33: 301-305, 1979.
14. IGRA-SIEGMAN, Y.; KAPILAR, R; SEN, P.; KAMINISKI, Z. C. & LOURIA, D. B. Syndrome of hyperinfection with Strongyloides stercoralis. Rev. infect. Dis., 3: 397-407, 1981.
15. KAMMERER, W. S. & MILLER, K. L. Echinococcus granulosus: permeability of hydatid cysts to mebendazole in mice. Int. J. Parasit., 11: 183-185, 1981.
16. KESSLER, W. & STOYE, M. Die wirjung neurerer Anthelminthinka auf migrierende Larven von Ancylostoma caninum in der Maus. Z. Tropenmed. Parasit., 22: 49-56, 1971.
17. LOUZADO, G. Z. Avaliação clínica da associação tinidazole + cambendazole + mebendazole no tratamento das helmintíases e das protozooses humanas mais freqüentes. Rev. bras. Med., 37: 645-649, 1980.
18. MALKIN, M. F. & CAMACHO, R. M. The effect of thiabendazole on fumarate reductase from thiabendazole-sensitive and resistant strains of Haemonchus contortus. J. Parasit., 58: 845-846, 1972.
IB. MARTIRANI, I. & RODRIGUES, L. D. Ensaio clínico com o cambendazole, uma nova droga na terapêutica anti-helmíntica (Nota previa). Rev. Inst. Med. trop. S. Paulo, 18: 71-75, 1976.
20. MUSGRAVE, I. A.; HAWES, R. B.; JAMESON, J. L.; SLOANE, R. A. & QUAYLE, P. A. Mebendazole. Evaluation of a new anthelmintic for trichuriasis, hookworm and strongyloidiasis. Med. J. Aust., 1: 403-405, 1979.
21. PANYATHANYA, R.; SRIUMPAI, S. & TANTRANOND, R. Fatal strongyloidiasis in Siriraj Hospital, Bangkok, Thailand. Southeast Asian. J. trop. Med. Puhl. Hith., 14: 294-297, 1983.
22. PRICHARD, R. K. The fumarate reductase reaction of Haemonchus contortus and the mode of action of some anthelmintics. Int. J. Parasit., 3: 409-417, 1973.
23. RODRIGUES, L. D.; MARTIRANI, I.; CABEÇA, M.; SOARES, W. & BRANDÃO, J. A. Cambendazole: Novo anti-helmíntico na terapêutica da estrongiloidíase. Experimentação clínica. Rev. Inst. Med. trop. S. Paulo, 19: 57-67, 1977.
24. SCOGGIN, C. H. & CALL, N. B. Acute respiratory failure due to disseminated strongyloidiasis in a renal transplant recipient. Ann. intern. Med., 87: 456-458, 1977.
25. SCOWDEN, E. B.; SCHAFFNER, W. & STONE, W J Overwhelming strongyloidiasis: an unappreciated opportunistic infection. Medicine (Baltimore), 57: 527 544, 1978.

Correspondence:

Dr. D. I. Grove

Department of Medicine

University of Western Australia

Queen Elizabeth II Medical Centre

Nedlands, Western Australia, 6009

Publication Dates

Publication in this collection
16 Oct 2012
Date of issue
Apr 1986

History

Received
22 Feb 1985

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AMATO NETO, V.; SINTO, T.; PEDRO, R. J.; LEVI, G. C. ; TSUKUMO, M. K.; MORAES, U. M. & CORREA, L. L. Nossas observações iniciais sobre a eficácia do cambendazole no tratamento da estrongiloidíase. Rev. Inst. Med. trop. S. Paulo, 20: 161-163, 1978.

[2] 2. BARANSKI, M. C; SILVA, A. P.; KOTAKA, P. I.; GOMES, N. R. ; GIOVANNI, M. & TELLES, J. E. Tratamento da, estrongiloidíase humana com nova droga anti-heimíntica, o cambendazole. Estudo duplo cego, Rev. Inst. Med. trop, S. Paulo, 20: 213-218, 1978.

[3] 3. BICALHO, S. A.; LEÃO, O. J. & PENA Jr., Q. Cambendazole in the treatment of human Strongyloidiasis. Amer. J. trop. Med. Hyg., 32: 1181-1183, 1983.

[4] 5. DAWKINS, H. J.; GEOVE, D. I.; DUNSMORE, J. E. & MITCHELL, G. F. Strongyloides ratti: susceptibility to infection and resistance to reinfection as assessed by excretion of larvae. Int. J. Parasit., 10: 125-129, 1380.

[5] 6. DESCHIENS, R. & BENEX, J. Essais expérimentaux ét cliniques de traitement de l'anguilullose intestinale par les sous-nitrate et par le carbonate de bismuth. Bull. Soc. Path. exot., 50: 70-74, 1957.

[6] 7. GROVE, D. I. Strongyloidiasis in Allied ex-prisoners of war in southeast Asia. Brit. med. J., 280: 598-601, 1980.

[7] 8. GROVE, D. I. Treatment of strongyloidiasis: an analysis of toxicity and. effectiveness. Trans. roy. Soc. trop. Med. Hyg., 76: 114-116, 1982.

[8] 9. GROVE, D. I. Strongyloides rattf and S. stereorails: the effects of thiabendazole, mebendazole and cambendazole in infected mice. Amer. J. trop. Med. Hyg., 31: 469-476, 1982.

[9] 10. GROVE, D. I. The effects of 22, 23-dihydroavermectin B₁ on Strongyloides ratti and S. stercoralis infections in mice. Ann. trop. Med. Parasit., 77: 405-410, 1983.

[10] 11. GROVE. D. I. & NORTHERN, C. Infection and immunity in dogs infected with a human strain of Strongyloides stercoralis. Trans. roy. Soc. trop. Med. Hyg., 76: 833-838, 1982.

[11] 12. HOFF, D. R.; FISHER, M. H.; BOCHIS, R. J.; LUSI, R. J.; WAKSMUNSKI, A.; EGERTON, R. J.; YASTIS, J. J.; CUCKLER, A. C. & CAMPBELL, W. C. A new broad spectrum anthelmintic: 2-(4-thiazolyl)-iso- propoxycarbonyl aminobenzimidazole. Experientia, 26: 550-552, 1970.

[12] 13. HUGGINS, D. Cambendazole-nova substância para o tratamento da estrongiloidíase. GEN, 33: 301-305, 1979.

[13] 14. IGRA-SIEGMAN, Y.; KAPILAR, R; SEN, P.; KAMINISKI, Z. C. & LOURIA, D. B. Syndrome of hyperinfection with Strongyloides stercoralis. Rev. infect. Dis., 3: 397-407, 1981.

[14] 15. KAMMERER, W. S. & MILLER, K. L. Echinococcus granulosus: permeability of hydatid cysts to mebendazole in mice. Int. J. Parasit., 11: 183-185, 1981.

[15] 16. KESSLER, W. & STOYE, M. Die wirjung neurerer Anthelminthinka auf migrierende Larven von Ancylostoma caninum in der Maus. Z. Tropenmed. Parasit., 22: 49-56, 1971.

[16] 17. LOUZADO, G. Z. Avaliação clínica da associação tinidazole + cambendazole + mebendazole no tratamento das helmintíases e das protozooses humanas mais freqüentes. Rev. bras. Med., 37: 645-649, 1980.

[17] 18. MALKIN, M. F. & CAMACHO, R. M. The effect of thiabendazole on fumarate reductase from thiabendazole-sensitive and resistant strains of Haemonchus contortus. J. Parasit., 58: 845-846, 1972.

[18] IB. MARTIRANI, I. & RODRIGUES, L. D. Ensaio clínico com o cambendazole, uma nova droga na terapêutica anti-helmíntica (Nota previa). Rev. Inst. Med. trop. S. Paulo, 18: 71-75, 1976.

[19] 20. MUSGRAVE, I. A.; HAWES, R. B.; JAMESON, J. L.; SLOANE, R. A. & QUAYLE, P. A. Mebendazole. Evaluation of a new anthelmintic for trichuriasis, hookworm and strongyloidiasis. Med. J. Aust., 1: 403-405, 1979.

[20] 21. PANYATHANYA, R.; SRIUMPAI, S. & TANTRANOND, R. Fatal strongyloidiasis in Siriraj Hospital, Bangkok, Thailand. Southeast Asian. J. trop. Med. Puhl. Hith., 14: 294-297, 1983.

[21] 22. PRICHARD, R. K. The fumarate reductase reaction of Haemonchus contortus and the mode of action of some anthelmintics. Int. J. Parasit., 3: 409-417, 1973.

[22] 23. RODRIGUES, L. D.; MARTIRANI, I.; CABEÇA, M.; SOARES, W. & BRANDÃO, J. A. Cambendazole: Novo anti-helmíntico na terapêutica da estrongiloidíase. Experimentação clínica. Rev. Inst. Med. trop. S. Paulo, 19: 57-67, 1977.

[23] 24. SCOGGIN, C. H. & CALL, N. B. Acute respiratory failure due to disseminated strongyloidiasis in a renal transplant recipient. Ann. intern. Med., 87: 456-458, 1977.

[24] 25. SCOWDEN, E. B.; SCHAFFNER, W. & STONE, W J Overwhelming strongyloidiasis: an unappreciated opportunistic infection. Medicine (Baltimore), 57: 527 544, 1978.

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Strongyloses ratti and S. stercoralis: effects of cambendazole, thiabendazole and mebendazole in vitro

Strongyloides ratti e S. stercoralis: efeitos de cambendazol, tiabendazol e mebendazol in vitro

Abstracts

Publication Dates

History